scholarly journals Pathogenicity of Fusarium avenaceum isolates to tulip leaves assessed on leaf disks

2013 ◽  
Vol 55 (1) ◽  
pp. 265-269
Author(s):  
Agnieszka Piwoni
Keyword(s):  
Gibberellic Acid ◽  
Leaf Senescence ◽  
Filter Paper ◽  
Fusarium Avenaceum ◽  
Abaxial Surface ◽  
Lesion Development ◽  
High Pathogenicity ◽  
Mean Size ◽  
Petri Dishes ◽  
Leaf Disks

The purpose of this study was to investigate the pathogenicity of 14 Fusarium avenaceum (Giberella avenacea) isolates, obtained from tulip plantations located in Puławy region, to leaves of tulip cv. Apeldoorn. Mycelial plugs 3 mm in diameter from the margins of the colonies growing on PDA and SNA were placed on 20 mm disks cut from tulip leaves. The disks were placed abaxial surface up, in Petri dishes on top of filter paper soaked with 100 mg/l gibberellic acid to retard leaf senescence. There were 5 replications for each isolate. Leaves in each Petń dish were inoculated with one isolate. Plates were incubated at 20°C and lesion diameters were measured after 4 days. The pathogenicity of investigated isolates was compared by measuring lesion development on tulip leaves. All of isolates colonized leaves disks succesfull - causing after 4 days necrotic lesions and sporulating after 14 days what indicated that an infection had occurred. There were significant differences in lesions size among isolates. Isolates: 27, 1, 2, 48 and 3 caused largest lesions at mean size from 150 mm<sup>2</sup> to 163 mm<sup>2</sup> what indicates their high pathogenicity. Less pathogenic were isolates: 28, 42, 7 and 72 caused lesions at mean size from 46 mm<sup>2</sup> to 97 mm~<sup>2</sup>. On average, mycelial plugs taken from PDA medium colonized leaf disks more efficient in comparison to them taken from SNA, that caused smaller lesions.

scholarly journals SEED PRODUCTIVITY AND DEVELOPMENT OF PLANTLETS POPULUS × JRTYSCHENSIS CH. Y. YANG

2019 ◽  
pp. 51-57
Author(s):  
B. V. Proshkin ◽  
A. V. Klimov
Keyword(s):  
Filter Paper ◽  
Fruit Set ◽  
Control Group ◽  
Natural Hybrid ◽  
Seed Productivity ◽  
Model Trees ◽  
Fruit Number ◽  
Black Poplar ◽  
Petri Dishes ◽  
Number Of Seeds

The research explores the seed productivity and plantlets growth in the free pollination of the natural hybrid taxon P. × jrtyschensis. Fruits of P. × jrtyschensis were selected from four plants that grow in the collection ofResearchCenter“EducationalBotanical Garden” ofKemerovoStateUniversity. Four P. nigra model trees, randomly selected from theTomRiverfloodplain population, were applied as a control group. The authors used 30 fruit-bearing amentumsfrom each model. The researchers measured set of fruit (capsule); number of ovules per fruit; number of seeds per fruit; set of seeds.. Laboratory germination was determined by sowing Petri dishes on wet filter paper. The authors found out sowing germination by sowing 100 seeds in a box with soil and drainage. The energy of germination was determined on the second day while germination - on the fifth day. P. × jrtyschensis is characterized by a lower level of seed productivity (15-30%) compared to P. nigra. In terms of laboratory germination of seeds, the descendants of hybrids surpassed many P. nigra models, but their soil germination was 20-30% lower than that of black poplar. The observed variability in reproductive indices of both P. × jrtyschensis and P. nigra is mainly caused by specific features of their genotypes. Plantlets being developed, the authors observed no significant differences among the descendants of P. nigra and hybrids. The researchers highlighted plantlets that can stop growing and even more abnormal plants with one, three or four seeds in P. × jrtyschensis. This may be caused by underdevelopment of hypocotyl or germ root. The authors observed breaches in development of P. nigra just once. They outline high plantlets destruction when sowing hybrids on the first day after germination The share of destructed plants within a month (from the beginning of the experiment) reaches 66,0 %, and in P. nigra it does not exceed 40,0 %.

scholarly journals Bioassay, Laboratory Tests of Beauveria Bassiana, Bacillus Thuringiensis, and a Feeding Stimulant on Leafroller Larvae, 1994

1995 ◽  
Vol 20 (1) ◽  
pp. 349-349
Author(s):  
J. F. Brunner ◽  
M. D. Doerr ◽  
L. O. Smith
Keyword(s):  
Beauveria Bassiana ◽  
Petri Dish ◽  
Larval Survival ◽  
Wetting Agent ◽  
Food Storage ◽  
Storage Container ◽  
Feeding Stimulant ◽  
Petri Dishes ◽  
Extension Center ◽  
Leaf Disks

Abstract Beauveria bassiana (Naturalis-L), two B. thuringiensis products (Dipel and Troy Bt), and a feeding stimulant (Konsume) combined with a B. thuringiensis product were evaluated using a leaf-dip bioassay method for their effect on PLR and OBLR larvae. Treatments were prepared by diluting the appropriate amt of product (see table) in 500 ml water in a glass beaker. The Konsume treatments received a constant 1X rate of Dipel and 4X-0.25X rates of Konsume. A small amt (about 2 ml) of wetting agent, X-77®, was added to each treatment. An untreated control was prepared using water plus the wetting agent only. Untreated apple leaves were collected from ‘Red Delicious’ trees at the WSU Tree Fruit Research and Extension Center, Wenatchee. Leaves were dipped, then allowed to dry. Two punches (2.3 cm diameter) were taken from each leaf. Four punches were placed in a petri dish (Falcon 1006, 50 × 9 mm). Petri dishes were chosen randomly, and five 1- to 2-d-old leafroller larvae were placed on the leaf disks. The petri dish lid was put in place and dishes were placed inside a food storage container and kept at 75°F (± 2°F) constant temperature and a photoperiod of 16:8 (L:D) h. Petri dishes were examined after 7 d and larval survival recorded. Ten dishes were used for each treatment (50 larvae per treatment).

scholarly journals Apple, Insecticide DIP Test (Tabm vs. Insecticides), 1993

1994 ◽  
Vol 19 (1) ◽  
pp. 369-369
Author(s):  
R. L. Horsburgh ◽  
J. R. Warren
Keyword(s):  
Filter Paper ◽  
Room Temperature ◽  
Petri Dish ◽  
Spray Application ◽  
Water Control ◽  
Poor Control ◽  
Laboratory Bioassay ◽  
The Third ◽  
Petri Dishes ◽  
Dip Test

Abstract A large commercial apple grower reported failure in attempts to control TABM with airblast applications of Penncap M (2 pt/acre) combined with methomyl (3 pt/acre). At the time of the grower applications larval hatch had begun and most larvae were in the first or second instar. By the time larvae were entering the third instar (10 days after spray application) it was apparent that commercially acceptable control had not been achieved. In 1992 this grower also had poor control when Penncap M was applied to suppress redbanded leafroller and experiments showed that tolerance of Penncap M was present in the resident RBLR population (Horsburgh et al. 1992). This laboratory bioassay was begun on 3 Sep to determine tolerance of the TABM population to Penncap M and other insecticides. Seven treatments (including a water control) were selected and rates calculated on the basis of 300 gal of spray being applied per acre. The appropriate dosage of pesticide for each treatment was mixed with 1 gallon of 77°F water in clean 1 gallon battery jars. Twenty plastic petri dishes containing moistened filter paper were prepared and served as individual cages for twenty 3rd instars per treatment. The larvae, on single leaves, were immersed in the appropriate solutions for five seconds and the leaf placed on the moist filter paper. The petri dish cover was put in place and the cages held at room temperature (80°F) for the duration of the test. All larvae were examined at 24 hour intervals and mortality was recorded. Death of larvae was assumed when no movement was observed when the larvae were gently prodded with a blunt steel probe.

scholarly journals Evaluation II of Insecticides for Control of Tarnished Plant Bugs in Mississippi, 1995

1996 ◽  
Vol 21 (1) ◽  
pp. 267-268
Author(s):  
Jack T. Reed ◽  
C. S. Jackson
Keyword(s):  
Science Research ◽  
Soil Conditions ◽  
Mortality Data ◽  
Plot Size ◽  
Fine Mesh ◽  
Initial Ph ◽  
Petri Dishes ◽  
Upper Third ◽  
Target Plant ◽  
Leaf Disks

Abstract Insecticides were evaluated at the Plant Science Research Farm, Mississippi State, MS, in a RCB design with 4 replications. Plot size was 1 row wide by 50 ft long with 7 unsprayed buffer rows between plots. Insecticides were applied on 27 Jun with a CO2-powered backpack sprayer equipped with a single Spraying Systems TX4 hollow cone nozzle and calibrated for 5 gpa at a pressure of 38 psi and a speed of 4 mph. Carrier of the insecticides was water, with an initial pH of 8.0. This trial was applied under very wet soil conditions, high humidity and steady wind conditions. The nozzle was held to allow the wind to drift the spray onto the plants with the upper third of the plant as the target. Plant bugs were collected the morning of application by sweeping wild flowers with a 15 inch diam sweep net. Fine mesh cloth cages were then placed on 2 plants per plot and fastened at the mainstem with ponytail holders. The plants with sleeve cages were cut and returned to the laboratory 24 h after infestation and examined for dead and live plant bugs. Because of threatening rain, instead of caging bugs on the plants, 4 leaf disks per plot were collected 48 h after spray application, and 2 leaf disks per plot 72 h after spray, placed in 11 X 50 mm petri dishes on moistened filter paper and infested with 5 plant bugs each. Mortality data from these petri dishes was taken at 96 h after insecticide application. Plant bugs not moving when prodded were considered dead.

Role of other plant organs in gibberellic acid-induced delay of leaf senescence in alstroemeria cut flowers

1993 ◽  
Vol 87 (3) ◽  
pp. 426-432 ◽  
Author(s):  
W. Jordi ◽  
H. M. Dekhuijzen ◽  
G. M. Stoopen ◽  
J. H. M. Overbeek
Keyword(s):  
Gibberellic Acid ◽  
Leaf Senescence ◽  
Cut Flowers ◽  
Plant Organs

scholarly journals pH Affects Seed Germination of Eight Bedding Plant Species

HortScience ◽  
1990 ◽  
Vol 25 (7) ◽  
pp. 762-764 ◽  
Author(s):  
Candice A. Shoemaker ◽  
William H. Carlson
Keyword(s):  
Plant Species ◽  
Filter Paper ◽  
Tagetes Patula ◽  
Species Response ◽  
Buffer Solutions ◽  
Ph Values ◽  
Salvia Splendens ◽  
Bedding Plant ◽  
Petri Dishes ◽  
Lobularia Maritima

Seeds of eight commonly grown bedding plant species [Ageratum houstonianum Mill., Begonia × semperflorens Hort., Impatiens wallerana Hook., Lobularia maritima (L.) Desv., Petunia × hybrida Hort., Pelargonium hortorum L.H. Bailey, Salvia splendens F. Sellow, Tagetes patula] were germinated at pH values from 4.5 to 7.5 at 0.5 increments. Seeds were germinated in petri dishes on filter paper saturated with buffer solutions or in petri dishes containing a 50 sphagnum peat: 50 coarse vermiculite (peatlite) medium moistened with buffer solutions. Germination on filter paper was affected by pH for all species tested. Peatlite medium pH affected germination of all species tested, except Salvia splendens. Species response to similar pH values differed between the two germination procedures. Total percent germination of seeds germinated was less in peatlite medium than on filter paper.

scholarly journals PHYSIOLOGICAL CHANGES ASSOCIATED WITH LEAF SENESCENCE IN EASTER LILIES

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 189a-189
Author(s):  
Rosanne E. Franco ◽  
Susan S. Han
Keyword(s):  
Gibberellic Acid ◽  
Leaf Senescence ◽  
Growth Regulators ◽  
Cold Storage ◽  
Foliar Application ◽  
Lilium Longiflorum ◽  
Continuous Treatment ◽  
Physiological Effects ◽  
Easter Lily ◽  
The Individual

Senescence of lower leaves of Easter lilies (Lilium longiflorum Thunb.) was previously shown to be delayed with application of the growth regulators, gibberellic acid (GA3) and benzyladenine (BA). This study was done to determine the physiological effects of GA3 and BA in relation to the delay of leaf senescence. Foliar application with 500 ppm BA or GA3 delayed chlorosis and lowered respiration rate in Easter lily leaves. A combination of 500 ppm BA and 500 ppm GA3 was more effective than the individual application of each. Gibberellic acid, BA, or their combination before cold storage resulted in delayed chlorosis and lowered respiration following removal from cold storage. Treatment with growth regulators after cold storage was less effective. Senescence of leaves was not associated with ethylene since ethylene production by leaves was undetectable by gas chromatograph. In addition, pulsing or continuous treatment with silver thiosulphate (STS), an inhibitor of ethylene synthesis, did not delay foliar chlorosis. Analysis of carbohydrate levels in Easter lily leaves treated with GA3, BA, or their combination may contribute to the understanding of the physiological effects of these two growth regulators.

scholarly journals A System for Controlling Water Potential in Seed Germination Research

HortScience ◽  
1992 ◽  
Vol 27 (4) ◽  
pp. 364-366 ◽  
Author(s):  
Phil S. Allen ◽  
Donald B. White ◽  
Karl Russer ◽  
Dave Olson
Keyword(s):  
Water Vapor ◽  
Seed Germination ◽  
Water Potential ◽  
Constant Temperature ◽  
Filter Paper ◽  
Water Reservoir ◽  
Vapor Pressures ◽  
Salt Solutions ◽  
Petri Dishes ◽  
Germinating Seeds

An inexpensive system for maintaining desired water potentials throughout seed germination was developed. During hydration, a water reservoir at the base of inclined petri dishes allowed continual saturation of filter paper on which seeds were placed. During dehydration, seeds were exposed to equilibrium vapor pressures above saturated salt solutions. Constant temperature, necessary to prevent condensation of water vapor, was achieved via a small (0.2 A) fan that furnished and circulated heat throughout an insulated chamber in which salt solutions were placed. By operating the chamber above ambient laboratory temperature, interior cooling was not required. The system allowed manipulation of the rate, degree, and frequency of dehydration episodes to which germinating seeds were exposed.

scholarly journals Anti-senescence Activity of Chemicals Applied to Kentucky Bluegrass

1990 ◽  
Vol 115 (4) ◽  
pp. 654-656 ◽  
Author(s):  
J.M. Goatley ◽  
R.E. Schmidt
Keyword(s):  
Exchange Rates ◽  
Leaf Senescence ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Growth Chamber ◽  
Distilled Water ◽  
Petri Dishes

This study was conducted to determine the potential anti-senescence activity of certain chemicals by monitoring changes in gross. CO2 exchange with senescence of excised leaves of Kentucky bluegrass (Poa pratensis L.). One day following foliar applications of benzyladenine (BA), triadimefon, and propiconazole, with and without chelated Fe (8% Fe phosphate citrate), Kentucky bluegrass leaves were excised, floated on distilled water in petri dishes, and placed in a darkened growth chamber. Gross CO2 exchange rates (CER) were recorded 1, 4, 7, and 10 days after excision (DAE). Foliar applications of Fe, BA, triadimefon, or propiconazole applied alone induced an anti-senescence response. Combinations of Fe with the chemicals delayed excision-induced leaf senescence, but no significant increase in anti-senescence activity was obtained from the Fe and chemical combinations as compared to the materials applied alone. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (benzyladenine, BA); 1-(4-Chlorophenoxy)-3,3-dimethyl-1(1H-1,2,4-triazol-1yl)-2-butanone (triadimefon);1-[[2-(2,4-dichlorophenyl)-4-propyl-1,3-dioxolan-2-yl]methyl]-1H-1,2,4-triazole (propiconazole).

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