scholarly journals Effect of cytokinins and amino acids on multiplication of Pelargonium cultivars

2014 ◽  
Vol 70 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Agnieszka Wojtania ◽  
Eleonora Gabryszewska

The effect of two cytokinins (BAP, m-Topolin) tested singly or in combination with a mixture of amino acids (glycine, glutamine, cysteine) on multiplication rate and quality of <em>Pelargonium</em> x <em>hederaefolium</em> and <em>Pelargonium</em> x <em>hortorum</em> shoots were investigated. For both <em>Pelargonium</em> cultivars, the application of cytokinins greatly increased the production of axillary and adventitious shoots compared with the shoots produced on the medium without growth regulators. In the case of <em>P</em>. x <em>hederaefolium</em> no significant differences it were found in the number of shoot formed for BAP and m-Topolin treatments. For <em>P</em>. x <em>hortorum</em> the most effective multiplication of shoots was obtained on the medium supplemented with m-Topolin (1.0 mg l<sup>-1</sup>). Moreover, in the presence of m-Topolin an improvement of shoot quality of both genotypes has been observed (well developed shoots with soft green leaves). It was particularly apparent for <em>P</em>. x <em>hortorum</em> shoots. The addition of amino acids to a cytokinin medium increased slightly the number of formed shoots of <em>P</em>. x <em>hederaefolium</em> and influenced the shoot quality of both <em>Pelargonium cultivars</em> (well developed leaf blades, uniform growth of shoots in clumps, slower process of shoot senescence).

2011 ◽  
Vol 79 (2) ◽  
pp. 101-106 ◽  
Author(s):  
Agnieszka Wojtania

July and September. The most efficient regeneration and axillary multiplication were achieved on the medium supplemented with meta-topolin. The application of BAP caused a lower regeneration potency of explants and resulted in a decrease of shoot quality with every subculture. Four of the six cultivars showed growth inhibition after three months of growth on BAP-medium. The highest multiplication rate (2.7-4.7 depending on genotype) and the high quality of shoots were noted on the medium supplemented with mT (0.5-1.0 mg l<sup>-1</sup>). It is also very important to note that mT had stimulating effect on organogenesis in <em>P.</em> × <em>hederaefolium</em> and <em>P.</em> × <em>hortorum</em> cultivars over the long term. Moreover, meta-topolin had no after-effect on the growth and inhibition of rooting. Only one cultivar ("Sofie Cascade") rooted better on control medium without auxin. In case of the other cultivars, IBA added in concentrations of 0.01-0.1 mg l<sup>-1</sup> had a stimulating effect on root production. The higher level of auxin inhibited root formation, stimulated senescence of shoots and had a negative after-effect on acclimatization in greenhouse conditions.


1978 ◽  
Vol 43 (6) ◽  
pp. 1695-1697 ◽  
Author(s):  
S. R. DRAKE ◽  
E. L. PROEBSTING ◽  
J. W. NELSON
Keyword(s):  

Author(s):  
C.S. Marchi ◽  
A.J.P. Albrecht ◽  
L.P. Albrecht ◽  
F.P. Novakoski ◽  
A.F.M. Silva ◽  
...  

Author(s):  
Rodica MARGAOAN ◽  
Liviu Alexandru MARGHITAS ◽  
Daniel Severus DEZMIREAN ◽  
Otilia BOBIS ◽  
Victorita BONTA ◽  
...  

Given their beneficial effects in terms of health, the natural products, especially beehive products, have drawn the attention of consumers since long time ago. In order to guarantee the quality of these products on the market, their chemical composition needs to be analyzed. Thus, this current research had as objective the establishment of quality parameters for beehive brood food derived products: apilarnil and queen bee larvae triturate. These two products were compared with royal jelly which is the basis of brood food in the first 3 days of larval stage. The carbohydrates were determined by HPLC-IR and allowed the identification of seven carbohydrate compounds, predominantly glucose, fructose and sucrose. The lipid profile was analyzed by the Soxhlet method. The total protein content was determined by the Kjeldahl method. Free amino acids were analyzed by LC-MS. A total of 31 amino acids were identified of which nine are essential amino acids for humans. 


2018 ◽  
Vol 30 (2) ◽  
pp. 259-267 ◽  
Author(s):  
Agnieszka Wojtania ◽  
Bożena Matysiak

Abstract The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown Rosa ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on in vitro initiation, multiplication and rooting of shoots were studied. The time of collecting explants from donor plants significantly affected the initiation of shoot culture of Rosa ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of Rosa ‘Konstancin’ plantlets, which were successfully acclimatized ex vitro, with a more than 90% survival rate.


2019 ◽  
pp. 155982761987404 ◽  
Author(s):  
Hamed Jafari-Vayghan ◽  
Jalal Moludi ◽  
Sevda Saleh-Ghadimi ◽  
Elgar Enamzadeh ◽  
Mir Hossein Seyed-Mohammadzad ◽  
...  

Background: Cardiac cachexia (CC) adversely affects the lifestyle of heart failure (HF) patients. The current study examined the impact of melatonin cosupplementation and branched-chain amino acids (BCAAs) on quality of life (QoL), fatigue, and nutritional status in cachectic HF patients. Methods: In this trial, 84 CC patients were randomized to melatonin, BCAAs, or coadministration (both) as intervention groups and a control group over 8 weeks. At baseline and postintervention, QoL, fatigue, and nutritional status were assessed. Results: After intervention, improvement in the overall and physical dimensions of QoL and appetite score were found to be statistically significant in the BCAAs (P < .001) and the melatonin+BCAAs (P < .001) groups compared with the placebo group. The emotional dimension score was significantly lower in the BCAAs group compared with the placebo group (P = .001). There was a statistically significant improvement in fatigue severity in all 3 intervention groups compared with the placebo group. The nutrition risk index (NRI) score increased significantly only in the melatonin group (P = .015), and there was no significant difference between the other groups (P = .804). Conclusions: Cosupplementation with BCAAs and melatonin improved QoL, fatigue status, and appetite in cachectic HF patients but did not affect NRI.


Author(s):  
Takehiro Okabayashi ◽  
Akihito Kozuki ◽  
Tatsuaki Sumiyoshi ◽  
Yasuo Shima

2016 ◽  
Vol 4 (4) ◽  
pp. 28
Author(s):  
Sabri Braha ◽  
Petrit Rama

The purpose of this research is to determine the impact of the turf-only substrate and turf–perlite in the ratio 2:1 and of growth regulators in the quality of adventive roots ( the number and length) of well lignified one-year old branches without fruit buds in the Bluecrop cultivar (Vaccinium corymbosum L.) taken at the end of the latent period before budding at the February 15 th during the -2015 growing season. In order to support the increase of the number of roots and their length the hardwood cuttings are treated with different IBA and NAA concentrations (1500, 3000, 4500 ppm), while a part of cuttings were untreated control. The number and the length of roots have increased in relation to the increase of concentration from 1500 to 3000 ppm followed by a decline of these values in concentrations over 3000 ppm. Respectively, the number of roots (8) and the higher values of root length (4.6 cm) are achieved in the turf–perlite substrate, IBA 3000 ppm (compared to the turf-only substrate). The presence of perlite helps the aeration of the substrate and supports biochemical and physiological processes which lead to the inducing of adventive roots. Regarding the number and length of roots an important variation for (p<0.05) was observed between different concentrations of IBA and NAA. In general the effect of IBA was a lot better than the effect of NAA.


Russian vine ◽  
2020 ◽  
Vol 14 ◽  
pp. 37-43
Author(s):  
I.A. Avdeenkо ◽  

Over the past few years in the Russian Federa-tion, especially in the Krasnodar Territory and Crimea, there has been an increase in the area of vine plantations. In this regard, carrying out additional operations that increase the rooting rate and quality of grape seedlings and cut-tings is a promising direction in nursery. The use of growth substances for rooting grape cuttings has proven to be the simplest and most economical way to achieve these goals. The paper analyzes the results of studies by various authors, proving the effectiveness of the use of growth substances on a grape plant.


Author(s):  
O. I. Ulyanych ◽  
◽  
I. A. Didenko ◽  
V. V. Yatsenko ◽  
◽  
...  

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