scholarly journals Production of Polyvinylpyrrolidone Nanowebs Containing Zinc Cyclohexane Mono Carboxylate via Electrospinning and Investigatıon of Antibacterial Effıciency

2019 ◽  
Vol 27 (6(138)) ◽  
pp. 91-96
Author(s):  
Aylin Yildiz ◽  
Riza Atav ◽  
Mine Aydin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Structure ◽  
Antibacterial Properties ◽  
The Other ◽  
Antibacterial Efficiency ◽  
E Coli ◽  
Other Hand ◽  
Edx Analyses

In this study, after the synthesis of zinc cyclohexane mono carboxylate, its chemical structure was analysed with FTIR and TGA. Then electrospun polyvinylpyrrolidone nanofibres containing zinc cyclohexane mono carboxylate were produced and antibacterial properties of the nanowebs obtained were investigated for their use in the textile field When the FTIR results of the nanofibres containing different concentrations of zinc cyclohexane monocarbocylate are examined, an –OH peak similar to that of PVP fibres is noticed. These results clearly indicate that zinc cyclohexane monocarboxylate is included in PVP. When the TGA spectra of CHMCZn-doped nanofibres at different ratios are examined, it is seen that they give more similar results than polyvinylprolidone nanofibres alone. According to the SEM-EDX analyses, it was observed that the fibre diameters obtained were in the range of 145 - 947 nm. On the other hand, antimicrobial activity against B. subtilis, S. aureus and E. coli strains was detected, found regardless of the CHMCZn concentration.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

PTCDA Films Deposited by Ionized Beam Method

1993 ◽  
Vol 316 ◽  
Author(s):  
Hiroaki Usui ◽  
Kiyoshi Kashihara ◽  
Kuniaki Tanaka ◽  
Seizo Miyata
Keyword(s):  
Thin Films ◽  
Molecular Orientation ◽  
Chemical Structure ◽  
Chemical Change ◽  
The Other ◽  
Ion Acceleration ◽  
Ionization Condition ◽  
Other Hand ◽  
Beam Method

ABSTRACTThin films of 3,4,9,10-perylenetetracarboxylic-dianhydride (PTCDA) were deposited by an ionized beam method. The molecular orientation and chemical structure of the films were studied in connection with ionization and ion acceleration conditions. At a low ionization condition, the molecules are oriented in parallel with the substrate, and the crystallinity was improved by an appropriate ion acceleration. At higher ionization conditions, on the other hand, ion acceleration resulted in a loss of crystallinity. Deposited molecules undergo chemical change in such a condition, leading to dissociation of dianhydride groups.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

scholarly journals Antibacterial and Antifungal Activity of the Human Endometrial Fluid during the Natural Cycle

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Marta Bregón-Villahoz ◽  
Maria-Dolores Moragues ◽  
Inés Arrieta-Aguirre ◽  
Mikel Azkargorta ◽  
Lucía Lainz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Activity Patterns ◽  
Antimicrobial Effect ◽  
The Other ◽  
Natural Cycle ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Related Pattern ◽  
Embryo Transfer Catheter

Purpose. Some microbiota patterns have been associated with favorable IVF prognosis and others with pathological conditions. The endometrial fluid aspirate (EFA) contains antibacterial proteins that are enriched in implantative IVF cycles, but the antimicrobial effect of EFA has not been addressed. We aimed to evaluate the antimicrobial activity of the human endometrial fluid during the natural cycle. Methods. EFA was obtained through an embryo transfer catheter in 38 women, aged 18-40 years, with regular cycles attending to a fertility clinic. The antimicrobial activity of EFAs was tested against two strains of Staphylococcus aureus; one strain each of Streptococcus agalactiae, Enterococcus faecalis, Escherichia coli, and Klebsiella pneumoniae; and three yeasts (Candida albicans, Candida glabrata, and Candida krusei). Results. All samples exhibited antibacterial activity against S. aureus. In addition, 32.4% of EFAs were active against one of the other microorganisms assayed, 16.2% against two, and 5.4% against four of them. In contrast, none exhibited antibacterial activity against E. coli or K. pneumoniae. The antimicrobial activity differs considerably between EFA samples, and we failed to observe a cycle-related pattern. Conclusions. EFA presented two antimicrobial activity patterns: (a) one common to all the samples, exhibiting activity against S. aureus and lack of activity against E. coli and K. pneumoniae, and (b) an individualized pattern, showing activity against some of the other microorganisms tested. The intensity of antibacterial activity differs between EFA samples. Our data suggest that the uterine microbiota is controlled by means of endometrial fluid components.

scholarly journals Synthesis and Antimicrobial Activity of 7-Hydroxy-3',4'-Methylenedioxy- and 7-Benzyloxy-3',4'-Methylenedioxy Flavanones

2017 ◽  
Vol 9 (3) ◽  
pp. 297-306 ◽  
Author(s):  
R. Ali ◽  
A. Rahim ◽  
A. Islam
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Elemental Analysis ◽  
The Other ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Antibacterial And Antifungal Activities ◽  
Other Hand ◽  
Antibacterial And Antifungal

7-Hydroxy-3',4'-methylenedioxy- and 7-benzyloxy-3',4'-methylenedioxy flavanones have been synthesized starting from 2,4-dihydroxyacetophenone. Subsequently biocidal activities of the flavanones have been investigated along with their corresponding chalcones against some bacterial and fungal strains. 2'-Hydroxy-4'-benzyloxy-3,4-methylenedioxy chalcone (5) and its corresponding flavanone (7) showed good antibacterial and antifungal activities against some selected bacterial and fungal strains. On the other hand, 2',4'-dihydroxy-3,4-methylenedioxy chalcone (4) showed no antibacterial and antifungal activities while its corresponding flavanone (6) showed a little antibacterial activity only at higher concentration but did not show antifungal activity. The synthesized chalcones and flavanones have been characterized using UV-Vis, IR and 1H NMRspectral data together with elemental analysis.

scholarly journals In vitro Synergistic Antimicrobial Activity of Romanian Propolis and Antibiotics against Escherichia coli Isolated from Bovine Mastitis

2015 ◽  
Vol 43 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Mihaela NICULAE ◽  
Laura STAN ◽  
Emoke PALL ◽  
Anamaria Ioana PAȘTIU ◽  
Iulia Maria BALACI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Bovine Mastitis ◽  
Antibacterial Properties ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts

The study was aimed to characterize the chemical composition and the antimicrobial activity of Romanian propolis ethanolic extracts (EEP) against antibiotic-sensitive and antibiotic-resistant E. coli strains isolated from bovine mastitis. The preliminary antimicrobial screening was performed by a disk diffusion method, followed by determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) based on broth microdilution assay; further, the synergistic action of propolis with antimicrobial drugs was assessed by a disk diffusion method on agar containing subinhibitory concentrations of propolis. For the chemical characterisation of EEP, the flavonoids (flavones/flavonols, flavanones/dihydroflavonols) and total phenolics were evaluated by spectrophotometric methods. The phenolic compounds of these extracts were also determined using HPLC. The results indicated for Romanian propolis ethanolic extracts the typical poplar composition profile with flavonoids and phenolic acids as main biological active compounds, with chromatographic analysis data confirmed also spectrophotometrically. In addition, positively correlated with the chemical composition, a strong antimicrobial efficacy was exhibited towards E. coli strains, along with interesting synergistic interaction with antibiotics that can be further investigated to obtain propolis-based formulation with antibacterial properties. Subsequent in vitro and in vivo studies evaluating the safety and efficacy are intended to consider propolis in veterinary therapeutic protocols.

Bacteria and Virus Removal from Secondary Effluent in Sand and Red Mud Columns

1991 ◽  
Vol 23 (1-3) ◽  
pp. 261-270 ◽  
Author(s):  
Goen E. Ho ◽  
Robyn A. Gibbs ◽  
Kuruvilla Mathew
Keyword(s):  
Escherichia Coli ◽  
Red Mud ◽  
Breakthrough Curves ◽  
The Other ◽  
Column Experiments ◽  
Secondary Effluent ◽  
Soil Columns ◽  
Virus Removal ◽  
E Coli ◽  
Other Hand

Column experiments were conducted to determine the improvement in the removal of Escherichia coli, Salmonella adelaide and poliovirus-1 through sands by amendment with bauxite refining residue. The residue (red mud) was neutralized using 5 % gypsum and incorporated to form 30% of the amended sands. In 65 cm long soil columns the removal of the three organisms in the amended sand columns was excellent with over seven orders of magnitude reduction in concentration. Removal in unamended sands was poor. From breakthrough curves in unamended sand columns filtration, die-off and adsorption all appear to play a role in organism removal. The results also show that E. coli can be used as an indicator for contamination, though S.adelaide was less efficiently removed than E. coli. Poliovirus was on the other hand better removed than E. coli.

In vitro O2′-methylation of sugars in E. coli RNA. III. Methylation of E. coli transfer RNA by heterologous methylases in particulate-free extracts of wheat embryo

1969 ◽  
Vol 47 (9) ◽  
pp. 863-869
Author(s):  
J. L. Nichols ◽  
B. G. Lane
Keyword(s):  
Transfer Rna ◽  
The Other ◽  
Wheat Embryo ◽  
E Coli ◽  
Pyrimidine Nucleosides ◽  
Other Hand

(1) Transfer ribonucleates (tRNA) from a relaxed strain of E. coli have been methylated by homologous enzymes from E. coli and by heterologous enzymes from wheat embryo, using particulate-free extracts supplemented with [methyl-14C]-S-adenosylmethionine.(2) For both homologous and heterologous systems, the methylation of bases greatly exceeded the methylation of sugars, although the patterns of base and sugar methylation were radically different for homologous and heterologous methylations. Thus, the primary targets for homologous methylation were pyrimidine nucleosides: ribothymidine, the primary product of base methylation, and O2′-methylcytidine in the sequence CmpU, the primary product of sugar methylation. On the other hand, the primary targets for heterologous methylation were purine nucleosides: N2,N2-dimethylguanosine, the primary product of base methylation, and O2′-methylguanosine in the sequence GmpG, the primary product of sugar methylation.(3) The results of an allied study of the methylation of wheat embryo tRNA by homologous wheat embryo methylases and heterologous E. coli methylases have also been reported.

scholarly journals Genetic Plurality of OXA/NDM-Encoding Features Characterized From Enterobacterales Recovered From Czech Hospitals

2021 ◽  
Vol 12 ◽  
Author(s):  
Katerina Chudejova ◽  
Lucie Kraftova ◽  
Vittoria Mattioni Marchetti ◽  
Jaroslav Hrabak ◽  
Costas C. Papagiannitsis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Risk ◽  
Klebsiella Pneumoniae ◽  
The Other ◽  
Travel History ◽  
Genetic Environment ◽  
E Coli ◽  
Resistance Determinants ◽  
Other Hand ◽  
Previous Hospitalization

The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged to “high risk” clones ST147, ST11, and ST15, while the Escherichia coli isolate was assigned to ST167. All isolates expressed resistance against most β-lactams, including carbapenems, while retaining susceptibility to colistin. Furthermore, analysis of WGS data showed that all four isolates co-produced OXA-48- and NDM-type carbapenemases, in different combinations (Kpn47733: blaNDM–5 + blaOXA–181; Kpn50595: blaNDM–1 + blaOXA–181; Kpn51015: blaNDM–1 + blaOXA–244; Eco52418: blaNDM–5 + blaOXA–244). In Kpn51015, the blaOXA–244 was found on plasmid p51015_OXA-244, while the respective gene was localized in the chromosomal contig of E. coli Eco52418. On the other hand, blaOXA–181 was identified on a ColKP3 plasmid in isolate Kpn47733, while a blaOXA–181-carrying plasmid being an IncX3-ColKP3 fusion was identified in Kpn50595. The blaNDM–1 gene was found on two different plasmids, p51015_NDM-1 belonging to a novel IncH plasmid group and p51015_NDM-1 being an IncFK1-FIB replicon. Furthermore, the blaNDM–5 was found in two IncFII plasmids exhibiting limited nucleotide similarity to each other. In both plasmids, the genetic environment of blaNDM–5 was identical. Finally, in all four carbapenemase-producing isolates, a diverse number of additional replicons, some of these associated with important resistance determinants, like blaCTX–M–15, arr-2 and ermB, were identified. In conclusion, this study reports the first description of OXA-244-producing Enterobacterales isolated from Czech hospitals. Additionally, our findings indicated the genetic plurality involved in the acquisition and dissemination of determinants encoding OXA/NDM carbapenemases.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF HONEY AGAINST AEROBIC AND ANAEROBIC BACTERIA

2021 ◽  
pp. 41-43
Author(s):  
S. DHANARAJ ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Clostridium Perfringens ◽  
Anaerobic Bacteria ◽  
The Other ◽  
E Coli ◽  
Diffusion Technique ◽  
Aerobic And Anaerobic

Objective: To compare the antibacterial activity of honey against aerobic and anaerobic bacteria. Methods: Honey is extracted from the honey comb by trained persons. Antimicrobial activity of honey is performed by Agar Cup Diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and Clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to honey whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to honey. Conclusion: Due to its vast antibacterial activity of honey, it can be used along with other antibiotics to increase its efficiency.

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