Statistical Correlation of the Soil Incubation and the Accelerated Laboratory Extraction Methods to Estimate Nitrogen Release Rates of Slow- and Controlled-Release Fertilizers

2014 ◽  
Vol 97 (3) ◽  
pp. 677-686 ◽  
Author(s):  
L Carolina Medina ◽  
Jerry B Sartain ◽  
Thomas A Obreza ◽  
Emily Leary ◽  
William L Hall ◽  
...  
Keyword(s):  
Controlled Release ◽  
Extraction Method ◽  
Nutrient Release ◽  
Extraction Methods ◽  
Statistical Correlation ◽  
Official Method ◽  
Soil Incubation ◽  
N Release ◽  
Production And Distribution ◽  
Release Curve

Abstract Several technologies have been proposed to characterize the nutrient release patterns of enhanced-efficiency fertilizers (EEFs) during the last few decades. These technologies have been developed mainly by manufacturers and are product- specific based on the regulation and analysis of each EEF product. Despite previous efforts to characterize nutrient release of slow-release fertilizer (SRF) and controlled-release fertilizer (CRF) materials, no official method exists to assess their nutrient release patterns. However, the increased production and distribution of EEFs in specialty and nonspecialty markets requires an appropriate method to verify nutrient claims and material performance. Nonlinear regression was used to establish a correlation between the data generated from a 180-day soil incubation-column leaching procedure and 74 h accelerated lab extraction method, and to develop a model that can predict the 180-day nitrogen (N) release curve for a specific SRF and CRF product based on the data from the accelerated laboratory extraction method. Based on the R2 > 0.90 obtained for most materials, results indicated that the data generated from the 74 h accelerated lab extraction method could be used to predict N release from the selected materials during 180 days, including those fertilizers that require biological activity for N release.

Evaluation of a Soil Incubation Method to Characterize Nitrogen Release Patterns of Slow- and Controlled-Release Fertilizers

2014 ◽  
Vol 97 (3) ◽  
pp. 643-660 ◽  
Author(s):  
L Carolina Medina ◽  
Jerry B Sartain ◽  
Thomas A Obreza ◽  
William L Hall ◽  
Nancy J Thiex
Keyword(s):  
Controlled Release ◽  
Release Rate ◽  
Nutrient Release ◽  
Soil Incubation ◽  
Release Rates ◽  
N Release ◽  
Production And Distribution ◽  
Slow Release Fertilizers ◽  
Incubation Temperatures ◽  
Controlled Release Fertilizers

Abstract Several technologies have been proposed to characterize the nutrient release patterns of slow- release fertilizers (SRF) and controlled-release fertilizers (CRF) during the last few decades. These technologies have been developed mainly by manufacturers, and are product-specific, based on the regulation and analysis of each SRF and CRF product. Despite previous efforts to characterize SRF and CRF materials, no standardized, validated method exists to assess their nutrient release patterns. However, the increased production and distribution of these materials in specialty and nonspecialty markets requires an appropriate method to verify product claims and material performance. A soil incubation column leaching procedure was evaluated to determine its suitability as a standard method to estimate nitrogen (N) release patterns of SRFs and CRFs during 180 days. The influence of three soil/sand ratios, three incubation temperatures, and four soils on method behavior was assessed using five SRFs and three CRFs. In general, the highest soil/sand ratio increased the N release rate of all materials, but this effect was more marked for the SRFs. Temperature had the greatest influence on N release rates. For CRFs, the initial N release rates and the percentage N released/day increased as temperature increased. For SRFs, raising the temperature from 25 to 35°C increased initial N release rate and the total cumulative N released, and almost doubled the percentage released/day. The percentage N released/day from all products generally increased as the texture of the soil changed from sandy to loamy (Iowa>California>Pennsylvania>Florida). The soil incubation technique was demonstrated to be robust and reliable for characterizing N release patterns from SRFs and CRFs. The method was reproducible, and variations in soil/sand ratio, temperature, and soil had little effect on the results.

Optimization and Validation of an Accelerated Laboratory Extraction Method to Estimate Nitrogen Release Patterns of Slow- and Controlled-Release Fertilizers

2014 ◽  
Vol 97 (3) ◽  
pp. 661-676 ◽  
Author(s):  
L Carolina Medina ◽  
Jerry B Sartain ◽  
Thomas A Obreza ◽  
William L Hall ◽  
Nancy J Thiex
Keyword(s):  
Controlled Release ◽  
Extraction Method ◽  
Nutrient Release ◽  
Portion Size ◽  
Effect Of Temperature ◽  
Extraction Temperature ◽  
Test Portion ◽  
N Release ◽  
Slow Release Fertilizers ◽  
Controlled Release Fertilizers

Abstract Several technologies have been proposed to characterize the nutrient release and availability patterns of enhanced-efficiency fertilizers (EEFs), especially slow-release fertilizers (SRFs) and controlled-release fertilizers (CRFs) during the last few decades. These technologies have been developed mainly by manufacturers and are product-specific based on the regulation and analysis of each EEF product. Despite previous efforts to characterize EEF materials, no validated method exists to assess their nutrient release patterns. However, the increased use of EEFs in specialty and nonspecialty markets requires an appropriate method to verify nutrient claims and material performance. A series of experiments were conducted to evaluate the effect of temperature, fertilizer test portion size, and extraction time on the performance of a 74 h accelerated laboratory extraction method to measure SRF and CRF nutrient release profiles. Temperature was the only factor that influenced nutrient release rate, with a highly marked effect for phosphorus and to a lesser extent for nitrogen (N) and potassium. Based on the results, the optimal extraction temperature set was: Extraction No. 1—2:00 h at 25°C; Extraction No. 2—2:00 h at 50°C; Extraction No. 3—20:00 h at 55°C; and Extraction No. 4—50:00 h at 60°C. Ruggedness of the method was tested by evaluating the effect of small changes in seven selected factors on method behavior using a fractional multifactorial design. Overall, the method showed ruggedness for measuring N release rates of coated CRFs.

scholarly journals Comparison of Babcock and Ether Extraction Methods for Determination of Fat Content of Cream: Collaborative Study

1996 ◽  
Vol 79 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Joanna M Lynch ◽  
David M Barbano ◽  
J Richard Fleming
Keyword(s):  
Extraction Method ◽  
Collaborative Study ◽  
Extraction Methods ◽  
Fat Content ◽  
Official Method ◽  
Ether Extraction ◽  
Aoac Official ◽  
The Difference ◽  
Aoac Official Method

Abstract A modified Mojonnier ether extraction method for determination of the fat content of cream was developed based on the method for milk (AOAC Official Method 989.05). The cream Babcock method (AOAC Official Method 920.111 B-C) was modified to harmonize with the milk Babcock method (AOAC Official Method 989.04) and to clarify procedural details. Using the AOAC collaborative study format, 10 laboratories tested 9 pairs of blind duplicate heat-treated cream samples with a fat range of 30-45% using both methods. The statistical performance (invalid and outlier data removed) was as follows: mean % fat = 37.932, sr = 0.125, sR = 0.151, RSDr = 0.330, RSDR = 0.398, r = 0.354, and R = 0.427 for the ether extraction method. For the Babcock method, mean % fat = 38.209, sr = 0.209, SR = 0.272, RSDr = 0.548, RSDR = 0.712, r = 0.592, and R = 0.769. Average test results for fat from the Babcock method were 0.277% (absolute fat) greater than for the Mojonnier ether extraction method. The difference between methods, as a percentage of the average fat content of the samples, was 0.73%. This agrees with differences observed between the 2 methods for milk when 10 to 17 laboratories tested 7 milk samples in blind duplicate at bimonthly intervals over a 4-year period (average difference 0.029% fat, 0.78% as a percentage of average fat content). The Mojonnier ether extraction and Babcock methods for fat in cream have been adopted by AOAC INTERNATIONAL. The new Babcock method replaced the AOAC Official Method 920.111 B-C.

scholarly journals Nitrogen Release Properties of Controlled-release Fertilizers during Tomato Production

HortScience ◽  
2014 ◽  
Vol 49 (12) ◽  
pp. 1568-1574 ◽  
Author(s):  
Luther C. Carson ◽  
Monica Ozores-Hampton ◽  
Kelly T. Morgan ◽  
Jerry B. Sartain
Keyword(s):  
Controlled Release ◽  
Nonlinear Regression ◽  
Nutrient Release ◽  
Nitrogen Release ◽  
Data Logger ◽  
Tomato Crop ◽  
Tomato Production ◽  
N Release ◽  
Soil Temperatures ◽  
Controlled Release Fertilizers

Determination of nutrient release duration from controlled-release fertilizers (CRFs) or soluble fertilizers encapsulated in polymer, resin, or sulfur covered fertilizer coated with a polymer differs among manufacturers, but may be determined as 75% to 80% nitrogen (N) release at a constant temperature (e.g., 20 to 25 °C). Increases or decreases in temperature compared with the manufacturer release determination temperature increase or decrease CRF N release; thus, coated fertilizer may release more rapidly than stated during the fall season when soil temperatures in seepage-irrigated tomato (Solanum lycopersicum) production can reach 40.1 °C. The objectives of this study were to evaluate N release duration of CRFs by measuring N release from CRFs incubated in pouches under polyethylene mulch-covered raised beds and to determine the CRF duration suitable for incorporation into a fall tomato fertility program. In 2011 and 2013, 12 and 14 CRFs from Agrium Advanced Technologies, Everris, Florikan, and Chisso-Asahi Fertilizer were sealed in fiberglass mesh pouches (12.7 × 14 cm) that were buried 10 cm below the bed surface in a tomato crop grown using commercial production practices. A data logger collected soil temperature 10 cm below the bed surface. Pouches were collected and N content was measured eight times through two fall seasons. A nonlinear regression model was fit to the data to determine N release rate. During the 2011 and 2013 seasons, minimum, average, and maximum soil temperatures were 21.2 and 19.2, 25.7 and 23.5, and 32.2 and 27.7 °C, respectively. Seasonal total CRF N release was between 77.6% and 93.8% during 2011 and 58.3% and 94.3% in 2013. In 2011, PCU90 and in 2013, PCU90 and PCNPK120 had the highest seasonal total percentage N release (PNR) and FL180 had the lowest in both years. A nonlinear regression fit N release from CRF with R2 = 0.85 to 0.99 during 2011 and 0.49 to 0.99 during 2013. Nitrogen release from all CRFs was faster than the manufacturer’s stated release, probably as a result of high fall bed temperatures. A CRF or CRF mixture containing CRFs of 120- to 180-day release duration may be recommended, but the CRFs must release greater than 75% N during the season.

Determination of Nitrogen, Phosphorus, and Potassium Release Rates of Slow- and Controlled-Release Fertilizers: Single-Laboratory Validation, First Action 2015.15

2016 ◽  
Vol 99 (2) ◽  
pp. 353-359 ◽  
Author(s):  
Nancy Thiex
Keyword(s):  
Controlled Release ◽  
Nutrient Release ◽  
Official Method ◽  
Release Rates ◽  
Expert Review ◽  
Expert Review Panel ◽  
Phosphorus And Potassium ◽  
Single Laboratory ◽  
Controlled Release Fertilizers

Abstract A previously validated method for the determination of nitrogen release patterns of slow- and controlled-release fertilizers (SRFs and CRFs, respectively) was submitted to the Expert Review Panel (ERP) for Fertilizers for consideration of First Action Official MethodSM status. The ERP evaluated the single-laboratory validation results and recommended the method for First Action Official Method status and provided recommendations for achieving Final Action. The 180 day soil incubation-column leaching technique was demonstrated to be a robust and reliable method for characterizing N release patterns from SRFs and CRFs. The method was reproducible, and the results were only slightly affected by variations in environmental factors such as microbial activity, soil moisture, temperature, and texture. The release of P and K were also studied, but at fewer replications than for N. Optimization experiments on the accelerated 74 h extraction method indicated that temperature was the only factor found to substantially influence nutrient-release rates from the materials studied, and an optimized extraction profile was established as follows: 2 h at 25°C, 2 h at 50°C, 20 h at 55°C, and 50 h at 60°C.

scholarly journals Initial Substrate Moisture Content Affects Chemical Properties of Bagged Substrates Containing Controlled Release Fertilizer at Two Different Temperatures

HortScience ◽  
2017 ◽  
Vol 52 (10) ◽  
pp. 1429-1434
Author(s):  
Ka Yeon Jeong ◽  
James E. Altland
Keyword(s):  
Controlled Release ◽  
Moisture Content ◽  
Storage Temperature ◽  
Chemical Properties ◽  
Nutrient Release ◽  
Initial Substrate ◽  
N Release ◽  
Different Temperatures ◽  
Substrate Moisture ◽  
Base Substrate

Bagged potting mixes can be stored for weeks or months before being used by consumers. Some bagged potting mixes are amended with controlled release fertilizers (CRFs). The objective of this research was to determine how initial substrate moisture content and storage temperature affect the chemical properties of bagged potting mix with CRF incorporated and stored for up to 180 days. The base substrate composed of 60 sphagnum peat: 30 bark : 10 perlite (by vol.) amended with 5.5 g·L−1 dolomitic limestone and 0.5 g·L−1 granular wetting agent. This base substrate was either not amended with additional fertilizer (control) or amended with 0.59 kg·m−3 N of a CRF (Osmocote 18N–1.3P–5K) that was either ground (CRF-G) or whole prills (CRF-P). Substrates had initial moisture contents (IMCs) of 25%, 45%, or 65% and were stored at temperatures of either 20 or 40 °C. IMC and fertilizer type affected pH, electrical conductivity (EC), and nutrient release. Substrate pH increased with increasing IMC due to greater lime reactivity. About 25% of N from CRF-G treatments was immobilized between 2 and 14 days of storage. Low moisture content of bags, due to low IMC or storage at 40 °C, reduced the rate of N release from CRF-P treatments. Substrate P was rapidly immobilized by microbial communities.

scholarly journals Solubility and Application Rate of Controlled-release Fertilizer Affect Growth and Nutrient Uptake in Containerized Woody Landscape Plants

1992 ◽  
Vol 117 (4) ◽  
pp. 578-583 ◽  
Author(s):  
Peter R. Hicklenton ◽  
Kenneth G. Cairns
Keyword(s):  
Controlled Release ◽  
Nutrient Release ◽  
Dry Weight ◽  
Application Rate ◽  
Laboratory Studies ◽  
Seasonal Growth ◽  
Landscape Plants ◽  
Rapid Release ◽  
Application Rates ◽  
N Release

Nutrient release from Nutricote Type 100 (100-day N release; 16N-4.4P-8.1K), and from a 1:3 mixture of Nutricote Type 40 (40-day N release; 16N-4.4P-8.1K) and Type 100 was affected by time and temperature. The Type 40/100 mixture released nutrients more rapidly over a 5 to 35C range in laboratory studies. Seasonal growth of containerized cotoneaster (Cotoneaster dammeri C.K. Schneid `Coral Beauty') and juniper (Juniperus horizontalis Moench. `Plumosa Compacta') increased with increasing application rates of either Nutricote Type 100 or a 1:3 mixture of Type 40/100 over the range 2-10 kg·m-3. Between 25 June and 27 July, cotoneaster grew more rapidly in media with Type 40/100 Nutricote, but by the end of the season (27 Sept.), fertilizer type showed no effect on plant dry weight. Shoot N was higher in cotoneaster plants grown with Type 40/100 Nutricote than with the Type 100 formulation during the first 2 months of growth, reflecting the more rapid release and uptake of N from the mixture. During the last month the situation was reversed, as nutrients from the Type 40/100 mixture were depleted. Potassium and P shoot concentrations were not affected by fertilizer type. Juniper growth and shoot concentrations of N, K, and P were not affected by fertilizer type at any time during the season. The results provided no evidence that seasonal growth could be enhanced in either cotoneaster (grows rapidly) or juniper (slower growing) by mixing rapid and more slowly releasing types of Nutricote.

scholarly journals Evaluation of DNA Extraction Methods Developed for Forensic and Ancient DNA Applications Using Bone Samples of Different Age

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 146
Author(s):  
Catarina Xavier ◽  
Mayra Eduardoff ◽  
Barbara Bertoglio ◽  
Christina Amory ◽  
Cordula Berger ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Ancient Dna ◽  
Extraction Method ◽  
Fragment Size ◽  
Molecular Genetic ◽  
Extraction Methods ◽  
Degraded Dna ◽  
Size Analysis ◽  
Dna Fragments ◽  
Dna Amount

The efficient extraction of DNA from challenging samples, such as bones, is critical for the success of downstream genotyping analysis in molecular genetic disciplines. Even though the ancient DNA community has developed several protocols targeting small DNA fragments that are typically present in decomposed or old specimens, only recently forensic geneticists have started to adopt those protocols. Here, we compare an ancient DNA extraction protocol (Dabney) with a bone extraction method (Loreille) typically used in forensics. Real-time quantitative PCR and forensically representative typing methods including fragment size analysis and sequencing were used to assess protocol performance. We used four bone samples of different age in replicates to study the effects of both extraction methods. Our results confirm Loreille’s overall increased gain of DNA when enough tissue is available and Dabney’s improved efficiency for retrieving shorter DNA fragments that is beneficial when highly degraded DNA is present. The results suggest that the choice of extraction method needs to be based on available sample, degradation state, and targeted genotyping method. We modified the Dabney protocol by pooling parallel lysates prior to purification to study gain and performance in single tube typing assays and found that up to six parallel lysates lead to an almost linear gain of extracted DNA. These data are promising for further forensic investigations as the adapted Dabney protocol combines increased sensitivity for degraded DNA with necessary total DNA amount for forensic applications.

scholarly journals Impact of Extraction Method on the Detection of Quality Biomarkers in Normal vs. DFD Meat

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1097
Author(s):  
Laura González-Blanco ◽  
Yolanda Diñeiro ◽  
Andrea Díaz-Luis ◽  
Ana Coto-Montes ◽  
Mamen Oliván ◽  
...  
Keyword(s):  
Extraction Method ◽  
Protein Pattern ◽  
Stress Protein ◽  
Electrophoretic Pattern ◽  
Extraction Methods ◽  
Sds Page ◽  
Weight Protein ◽  
Triton X ◽  
Protein Extractability ◽  
Selection Of

The objective of this work was to demonstrate how the extraction method affects the reliability of biomarker detection and how this detection depends on the biomarker location within the cell compartment. Different extraction methods were used to study the sarcoplasmic and myofibrillar fractions of the Longissimus thoracis et lumborum muscle of young bulls of the Asturiana de los Valles breed in two quality grades, standard (Control) or dark, firm, and dry (DFD) meat. Protein extractability and the expression of some of the main meat quality biomarkers—oxidative status (lipoperoxidation (LPO) and catalase activity (CAT)), proteome (SDS-PAGE electrophoretic pattern), and cell stress protein (Hsp70)—were analyzed. In the sarcoplasmic fraction, buffers containing Triton X-100 showed significantly higher protein extractability, LPO, and higher intensity of high-molecular-weight protein bands, whereas the TES buffer was more sensitive to distinguishing differences in the protein pattern between the Control and DFD meat. In the myofibrillar fraction, samples extracted with the lysis buffer showed significantly higher protein extractability, whereas samples extracted with the non-denaturing buffer showed higher results for LPO, CAT, and Hsp70, and higher-intensity bands in the electrophoretic pattern. These findings highlight the need for the careful selection of the extraction method used to analyze the different biomarkers considering their cellular location to adapt the extractive process.

scholarly journals Preparation and Sustained-Release Performance of PLGA Microcapsule Carrier System

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1758
Author(s):  
Shuaikai Ren ◽  
Chunxin Wang ◽  
Liang Guo ◽  
Congcong Xu ◽  
Yan Wang ◽  
...  
Keyword(s):  
Controlled Release ◽  
Biomedical Applications ◽  
Encapsulation Efficiency ◽  
Drug Carriers ◽  
Optimum Conditions ◽  
Carrier System ◽  
Preparation Conditions ◽  
Encapsulation Rate ◽  
Release Drug ◽  
Release Curve

Microcapsules have been widely studied owing to their biocompatibility and potential for application in various areas, particularly drug delivery. However, the size of microcapsules is difficult to control, and the size distribution is very broad via various encapsulation techniques. Therefore, it is necessary to obtain microcapsules with uniform and tailored size for the construction of controlled-release drug carriers. In this study, emulsification and solvent evaporation methods were used to prepare a variety of ovalbumin-loaded poly (lactic-co-glycolic acid) (PLGA) microcapsules to determine the optimal preparation conditions. The particle size of the PLGA microcapsules prepared using the optimum conditions was approximately 200 nm, which showed good dispersibility with an ovalbumin encapsulation rate of more than 60%. In addition, porous microcapsules with different pore sizes were prepared by adding a varying amount of porogen bovine serum albumin (BSA) to the internal water phase. The release curve showed that the rate of protein release from the microcapsules could be controlled by adjusting the pore size. These findings demonstrated that we could tailor the morphology and structure of microcapsules by regulating the preparation conditions, thus controlling the encapsulation efficiency and the release performance of the microcapsule carrier system. We envision that this controlled-release novel microcapsule carrier system shows great potential for biomedical applications.

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