The Validation of the Dai Nippon Medi·Ca SA Method for AOAC Research Institute Performance Tested MethodsSM Certification

2018 ◽  
Vol 101 (5) ◽  
pp. 1522-1533
Author(s):  
Mai Shimizu ◽  
Kentaro Takenaka ◽  
Takeo Suzuki ◽  
Aya Miyasaka ◽  
Taiki Matsuda ◽  
...  
Keyword(s):  
Reference Method ◽  
Sample Volume ◽  
Contamination Level ◽  
Stability Studies ◽  
Cooked Ham ◽  
Aoac Official ◽  
The Mean ◽  
Food Matrices ◽  
Mean Differences ◽  
Medium Method

Abstract A ready-made dry medium method for Staphylococcus aureus count, the Medi·Ca SA method incubated at 35 or 37°C, was compared with the Baird-Parker method (AOAC Official MethodSM975.55) for 11 food matrices: raw beef, raw ground beef, raw lamb, cooked ham, raw salmon, frozen prawn, fresh chilled pasta, pasteurized milk, natural cheese, cream puff, and potato salad. The mean difference between the two methods at each contamination level for each matrix was <0.5 log10, and the 95% confidence intervals on the mean differences fell within the range of −0.50 to 0.50. Standard deviation of repeatability and RSDr values of the Medi·Ca SA method were generally the same level as those of the Baird-Parker method, and r2 ranged from 0.98 to 1.00. Product consistency and stability studies showed little variability between productions lots and a shelf-life of 16 months. Incubation time within the range of 22–26 h and variations to the sample volume did not adversely affect the results. These results showed that the Medi·Ca SA method is a reasonable alternative to the reference method for selected food matrices and makes it possible to simultaneously detect and enumerate S. aureus in only 24�h.

Validation of Medi·Ca EC Method for AOAC Research Institute Performance Tested MethodsSM Certification

2017 ◽  
Vol 100 (2) ◽  
pp. 474-484
Author(s):  
Mai Shimizu ◽  
Fumihiko Saito ◽  
Kentaro Takenaka ◽  
Narumi Kimura ◽  
Takeo Suzuki ◽  
...  
Keyword(s):  
Reference Method ◽  
Sample Volume ◽  
Most Probable Number ◽  
Contamination Level ◽  
Probable Number ◽  
E Coli ◽  
Cooked Ham ◽  
The Mean ◽  
Mean Differences ◽  
Medium Method

Abstract A ready-made dry medium method for Escherichia coli and coliform count, the Medi·CaEC method, was compared with the most probable number (MPN) method using Brilliant Green Lactose Bile broth and E. coli broth (AOAC INTERNATIONAL Official MethodSM966.24) for seven food matrixes: raw beef, raw pork, raw frozen pork, raw lamb, raw salmon, frankfurter sausage, and cooked ham. The mean difference between the two methods at each contamination level for each matrix was <0.5 log10, and the 95% confidence intervals for the mean differences fell within the range of –0.5 to 0.5, with the exception of a few cases in the independent laboratory study. sr andRSDr values of the Medi·Ca EC method were generally lower than those of the MPN method, and r2 ranged from 0.91 to 0.99. Product consistency and stability studies showed little variability between production lots and the shelf-life of 20 months. An incubation time within the range of 22–26 hdid not adversely affect the results; however, variations in sample volume did affect final counts. These results showed that the Medi·Ca EC method is a reasonable alternative to the reference method forthe selected food matrixes and makes it possible to simultaneously detect and enumerate E. coli and coliform in only 24 h.

Dai Nippon Printing Co., Ltd Medi·Ca CC for Enumeration of Coliform Bacteria

2015 ◽  
Vol 98 (1) ◽  
pp. 62-70
Author(s):  
Fumihiko Saito ◽  
Mai Shimizu ◽  
Takeo Suzuki ◽  
Chie Hamada ◽  
Tatsuhiko Iwase ◽  
...  
Keyword(s):  
Reference Method ◽  
Coliform Bacteria ◽  
Contamination Level ◽  
Salmon Fillet ◽  
Fresh Cut ◽  
Raw Foods ◽  
Contamination Levels ◽  
Raw Poultry ◽  
Raw Shrimp ◽  
Medium Method

Abstract A ready-made dry medium method for coliform count, the Medi·Ca CC method, was compared to the Violet Red Bile Agar method (BacteriologicalAnalytical Manual, Chapter 4, Enumeration of Escherichia coli and the ColiformBacteria, Section G) for nine raw foods from four food categories: raw ground pork, raw lamb, raw groundchicken, raw tuna fillet, raw salmon fillet, raw shrimp, fresh peeled banana, fresh cut pineapple, and fresh cut apple. The 95% confidence interval for the mean difference between the two methods at each contamination level for seven matrixes from all four categories fell within the range of –0.50 to 0.50, and no statistical difference was observed at allthree contamination levels for four matrixes from three categories. These results demonstrated that the Medi·Ca CC method is a reasonable alternativeto the reference method for raw meat, raw poultry, raw fish, and fresh fruits.

scholarly journals Performance and Specificity of Monoclonal Immunoassays for Cyclosporine Monitoring: How Specific Is Specific?

1999 ◽  
Vol 45 (3) ◽  
pp. 371-381 ◽  
Author(s):  
Werner Steimer
Keyword(s):  
Comprehensive Evaluation ◽  
Reference Method ◽  
Hepatic Function ◽  
Cross Reactivity ◽  
Specific Reference ◽  
Clinical Specimens ◽  
New Methods ◽  
The Mean ◽  
Factors Of Influence ◽  
Mean Differences

Abstract Background: Immunoassays designed for the selective measurement of cyclosporin A (CsA) inadvertently show cross-reactivity for CsA metabolites. The extent and clinical significance of the resulting overestimation is controversial. A comprehensive assessment of old and new methods in clinical specimens is needed. Methods: In a comprehensive evaluation, CsA was analyzed in 145 samples with the new CEDIA® assay and compared with the Emit® assay with the old and new pretreatments, the TDx® monoclonal and polyclonal assays, the AxSYM®, and HPLC. All samples were from patients with liver and/or kidney transplants. Results: The CEDIA offered the easiest handling, followed by the AxSYM, which showed the longest calibration stability. The TDx monoclonal assay provided the lowest detection limit and the lowest CVs. The mean differences compared with HPLC were as follows: Emit, 9–12%; CEDIA, 18%; AxSYM, 29%; and TDx monoclonal, 57%. The CycloTrac® RIA paralleled the Emit results. In contrast to the mean differences, substantial (>200%) and variable overestimations of the CsA concentration were observed in individual patient samples. Metabolic ratios, estimates of the overall concentrations of several cross-reacting metabolites (nonspecific TDx polyclonal/specific reference method), correlated with the apparent biases of the various monoclonal assays. Metabolic ratios varied up to 10-fold, which translated into biases for individual samples between −7% and +174%. The higher the cross-reactivity of an assay was, the higher was the range of biases observed. The interindividual differences markedly exceeded other factors of influence (organ transplanted, hepatic function). Conclusion: Because assay bias cannot be predicted in individual samples, substantially erratic CsA dosing can result. The specificity of CsA assays for parent CsA remains a major concern.

scholarly journals Validation Study of Immunoaffinity Column Chromatography Coupled with Solution Fluorometry or HPLC for the Detection of Aflatoxin in Peanuts and Corn

2011 ◽  
Vol 94 (2) ◽  
pp. 572-588 ◽  
Author(s):  
Anthony Lupo ◽  
Anna Quain ◽  
Alan Fitzsimmons ◽  
Andrew Allan ◽  
Bert Popping ◽  
...  
Keyword(s):  
Validation Study ◽  
Reference Method ◽  
Reference Value ◽  
Immunoaffinity Column ◽  
Total Aflatoxin ◽  
Rapid Methods ◽  
Relative Recovery ◽  
Aoac Official ◽  
The Mean ◽  
Research Institute

Abstract Neogen Corp. has developed the Neocolumn for Aflatoxin DR for the detection of total aflatoxin by HPLC or solution fluorometry. The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested MethodsSM (PTM) program. There are several AOAC Official MethodsSM for detection of total aflatoxin in corn; they consist of rapid and analytical-based methods and two rapid methods (PTMs 030701 and 050901) that have been performance tested by the AOAC Research Institute. A widely used reference method, however, is AOAC Official MethodSM 991.31, which uses immumoaffinity cleanup followed by HPLC or solution fluorometry and is referred to as the reference method in this document. In internal studies, the Neocolumn method coupled with solution fluorometry demonstrated a relative recovery from peanuts of 101.6 of the reference value, with a CV of 3.9 across all levels analyzed; when coupled with HPLC, the Neocolumn method demonstrated a relative recovery from peanuts of 103.0 of the reference value with a CV of 3.5 across all levels analyzed. The Neocolumn method coupled with solution fluorometry demonstrated a relative recovery from corn of 116.9 of the reference value with a CV of 6.1 across all levels analyzed; when coupled with HPLC, the Neocolumn method demonstrated a relative recovery from corn of 91.2 of the reference value, with a CV of 5.4 across all levels analyzed. Calculations were made by comparison with the mean result obtained by the HPLC reference method, which showed respective CV values of 3.9 and 2.0 for recoveries from peanuts and corn, respectively.

Dai Nippon Printing Co., Ltd, Medi·Ca AC for Enumeration of Aerobic Bacteria

2014 ◽  
Vol 97 (3) ◽  
pp. 837-842
Author(s):  
Norihiko Okochi ◽  
Mamoru Yamazaki ◽  
Shoichi Kiso ◽  
Mai Kinoshita ◽  
Yurie Okita ◽  
...  
Keyword(s):  
Meat Products ◽  
Contamination Level ◽  
Processed Meat ◽  
Official Method ◽  
Pork Sausage ◽  
Microbiological Methods ◽  
Cooked Ham ◽  
Contamination Levels ◽  
Cooked Meat ◽  
Medium Method

Abstract A ready-made dry medium method for aerobic count, the Medi·Ca AC method, was compared to the AOAC Official Method 966.23, Microbiological Methods, for seven different heat-processed meat matrixes: cooked roast beef, Chinese barbecued pork (barbecued pork seasoned with honey-based sauce), bacon, cooked ham, frankfurter (made from beef and pork), and boiled and cooked pork sausage. The 95% confidence interval for the mean difference between the two methods at each contamination level for each matrix fell within the range of −0.50 to 0.50, and no statistical difference was observed at all three contamination levels for five matrixes. These results demonstrate that the Medi·Ca AC method is a reasonable alternative to the AOAC 966.23 method for cooked meat products.

scholarly journals Effectiveness of Home-Based Occupational Therapy on COPM Performance and Satisfaction Scores in Patients with COPD

2020 ◽  
pp. 000841742097112
Author(s):  
Eleonore H. Koolen ◽  
Martijn A. Spruit ◽  
Marianne de Man ◽  
Jeanine C. Antons ◽  
Elieke Nijhuis ◽  
...  
Keyword(s):  
Occupational Therapy ◽  
Integrated Management ◽  
Performance Measure ◽  
Occupational Performance ◽  
Post Intervention ◽  
Canadian Occupational Performance Measure ◽  
Home Based ◽  
Copd Patients ◽  
The Mean ◽  
Mean Differences

Background. Occupational therapy (OT) may be an important intervention in patients with COPD, but studies show conflicting results. Purpose. To evaluate the effectiveness of home-based monodisciplinary OT in COPD patients Method. We conducted an observational clinical study. Main outcomes were the mean differences in the Canadian Occupational Performance Measure (COPM) performance and satisfaction scores, pre and post intervention. Findings. Pre- and postintervention data were obtained from 41 patients. Statistically significant increases were observed in COPM performance (5.0 ± 1.1 versus 6.9 ± 0.9; P<0.001) and satisfaction (4.6 ± 1.3 versus 6.9 ± 1.0; P<0.001). The most frequently reported occupational performance problems were found in the domains of productivity (47%) and mobility (40%), fewer in self-care (10%) and the least in leisure (3%). Implications. Home-based monodisciplinary OT can contribute significantly to the improvement of daily functioning of patients with COPD. OT should therefore be considered more often as part of the integrated management of these patients.

scholarly journals The reproducibility of subjective appetite scores

1995 ◽  
Vol 73 (4) ◽  
pp. 517-530 ◽  
Author(s):  
Anne Raben ◽  
ANNA TAGLIABUE ◽  
Arne Astrup
Keyword(s):  
Visual Analogue Scale ◽  
Food Consumption ◽  
Analogue Scale ◽  
Prior Experience ◽  
Random Noise ◽  
Normal Weight ◽  
Vas Scores ◽  
The Mean ◽  
The Difference ◽  
Mean Differences

Although subjective appetite scores are widely used, studies on the reproducibility of this method are scarce. In the present study nine healthy, normal weight, young men recorded their subjective appetite sensations before and during 5 h after two different test meals A and B. The subjects tested each meal twice and in randomized order. Visual analogue scale (VAS) scores, 10 cm in length, were used to assess hunger, satiety, fullness, prospective food consumption and palatability of the meals. Plasma glucose and lactate concentrations were determined concomitantly. The repeatability was investigated for fasting values, Δ-mean 5 h and mean 5 h values, Δ-peak/nadir and peak/nadir values. Although the profiles of the postprandial responses were similar, the coefficients of repeatability (CR = 2SD) on the mean differences were large, ranging from 2·86 to 5.24 cm for fasting scores, 1·36 to 1·88 cm for mean scores, 2·98 to 5·42 cm for Δ-mean scores, and 3·16 to 6·44 cm for peak and Δ-peak scores. For palatability ratings the CK values varied more, ranging from 2·38 (taste) to 8·70 cm (aftertaste). Part of the difference in satiety ratings could be explained by the differences in palatability ratings. However, the low reproducibility may also be caused by a conditioned satiation or hunger due to the subjects' prior experience of the meals and therefore not just be a reflection of random noise. It is likely, however, that the variation in appetite ratings is due both to methodological day-to-day variation and to biological day-to-day variation in subjective appetite sensations.

scholarly journals Effectiveness of orthodontic temporary anchorage devices in canine retraction and anchorage preservation during the two-step technique: a systematic review and meta-analysis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Haonan Tian ◽  
Congman Xie ◽  
Min Lin ◽  
Hongmei Yang ◽  
Aishu Ren
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Risk Of Bias ◽  
Controlled Trials ◽  
Cochrane Central Register ◽  
Canine Retraction ◽  
Temporary Anchorage Devices ◽  
The Mean ◽  
Mean Differences ◽  
Implant Anchorage

Abstract Background Temporary anchorage devices have been used for decades in orthodontic practice for many applications. The aim of this systematic review was to assess the effectiveness of orthodontic temporary anchorage devices in canine retraction during the two-step technique. Methods A search was systematically performed for articles published prior to June 30, 2019 in five electronic databases (PubMed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, Scopus). The risk of bias was assessed using the Cochrane risk of bias tool for randomized controlled trials (RCTs) and the risk of bias in nonrandomized studies of interventions (ROBINS-I) tool for controlled clinical trials (CCTs). The Grading of Recommendation, Assessment, Development and Evaluation (GRADE) approach was used for the quality assessment. Data concerning the mean difference in mesial molar movement and extent of canine retraction were extracted for statistical analysis. The mean differences and 95% confidence intervals were analyzed for continuous data. A meta-analysis with a random-effects model for comparable outcomes was carried out. Results Three RCTs and five CCTs were finally included. Meta-analysis showed a significant increase not only in anchorage preservation in the implant anchorage group in both the maxilla (1.56 mm, 95% CI: 1.14 to 1.98, P < 0.00001) and the mandible (1.62 mm, 95% CI: 1.24 to 2.01, P < 0.00001) but also in canine retraction in the implant anchorage group in both the maxilla (0.43 mm, 95% CI: 0.16 to 0.69, P = 0.001) and the mandible (0.26 mm, 95% CI: 0.02 to 0.49, P = 0.03). Conclusions There is very low-quality evidence showing that implant anchorage is more efficient than conventional anchorage during canine retraction. Additional high-quality studies are needed.

scholarly journals Personality of ‘Only Children’ and ‘Children with a Sibling’

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Jaya Eknath Badi
Keyword(s):  
College Students ◽  
Family Structure ◽  
Ocean Model ◽  
T Test ◽  
Joint Family ◽  
Nuclear Families ◽  
Only Children ◽  
Five Factors ◽  
The Mean ◽  
Mean Differences

Nowadays in India, a social and family structure is changing rapidly. Society is shifting from its traditional joint family structure to nuclear families. In last few years, it is observed that couples prefer to keep their family smaller and many of them even prefer to have only one child. The current study tries to explore the effect of being single or having a sibling on the personality of children. Following the OCEAN model of personality, NEO-FFI was administered on 200 college students. 50 girls and 50 boys without a sibling and 50 girls and 50 boys with one sibling were selected from senior colleges in Pune city. By using ‘t’ test the mean differences were statistically computed. Results showed that there are no significant differences between only children and children with a sibling on all five factors of personality (Openness, Conscientiousness, Extraversion, Agreeableness, and Neuroticism).

scholarly journals Strengths among Contemporary Teenagers: A Comparative Study

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Ritu Rani ◽  
Punam Midha ◽  
Ankita Budhiraja
Keyword(s):  
Harmonious Society ◽  
Descriptive Statistics ◽  
Female Adolescents ◽  
Male And Female ◽  
Signature Strengths ◽  
Fast Speed ◽  
The Mean ◽  
Mean Differences ◽  
Age Range ◽  
Purposive Sample

Male and female are two indispensible wheels of a chariot, thus both complement each other and this is a fact that without any one of them, it is just impossible to imagine the smooth continuation of the journey of life. India is a country where women were worshiped as Goddess. Though the society in which we live has been shaped historically by males. But recently the existing scenario appears to be changing with the realization of strengths among females. A harmonious society appears to be emerging with the fast speed of decline in gender-specificity. In order to empirically confirm these notions, the present researchers decided to explore and compare the profile of strengths of male-female adolescents. A test of Hindi adaptation of Value In Action (VIA) Inventory of Strengths for Youth by Shree and Singh (2013) was used. A purposive sample of 150 adolescents in which 75 were males and 75 were females with the age range of 13-17 years was drawn from Rohtak city, Haryana. The descriptive statistics was used to find out the mean differences in strengths of male and female adolescents. They differed to some extent in their profile of signature strengths. Further the profile of top five strengths also called “signature strengths”, the male and female differed in two out of five signature strengths. The similar signature strengths found in both male and female adolescents were such as judgment/critical thinking, appreciation of beauty and love, however they differed on two strengths such as zest and perspective were found in male while kindness and modesty were found in female. The inference drawn from the current study is that females are more humane than the male counterpart whereas males are found to be more courageous than females. The need to foster or enhance certain strengths in specific gender are discussed in the paper.

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