scholarly journals Evaluation of eggplant and gilo genotypes and interspecific hybrids as to root-knot nematode resistance

2021 ◽  
Vol 17 (2) ◽  
pp. 30-38
Author(s):  
Jadir Borges Pinheiro ◽  
Giovani Olegario da Silva ◽  
Jhenef Gomes de Jesus ◽  
Danielle Biscaia ◽  
Raphael Augusto de Castro e Melo
Keyword(s):  
Interspecific Hybrids ◽  
Nematode Resistance ◽  
Small Scale ◽  
Production Volume ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Completely Randomized Design ◽  
Significant Production

In Brazil, eggplant and gilo are important for the economy of small-scale farms located mainly in the southeast states and other regions, with a significant production volume year-round in the wholesale local markets. However, these species are very susceptible to root-knot nematodes, and there are few or almost none known sources of resistance. The objective of this studywas to prospect sources of resistance to root-knot nematodesin eggplant, scarlet eggplant (gilo), as well in interspecific hybrids between these species and with wildSolanumspecies, to be used as rootstocks. In the first experiment, in 2013,10 eggplant accessions, a hybrid between eggplant andgilo, and a Solanum stramonifoliumxeggplanthybrid, were evalu41atedfor theirreaction to Meloidogyne enterolobii. In the second, in 2016, 20 accessions of gilowere evaluated for their reaction to M. incognita, M. javanica,and M. enterolobii.. And in the third experiment,in 2017,one access and two experimental eggplant hybrids, and one Solanum scuticumx eggplant hybrid, were evaluated for their reaction to M. incognita, and M. enterolobii. All the trials were stablished inagreenhouse, and characters related to root infection were evaluated in a completely randomized design with six replications of one plant per pot, usinga 1.5 L pots filled with a mixedsubstrate inoculated with each nematode species.Itwas found thatall eggplant accessions were susceptible to M. incognitaand M. enterolobii, however, BER 3150 presented lower susceptibility to M. incognita. The gilogenotypes CNPH 056, CNPH 070, CNPH 220,and CNPH 363 shownbetter response to M. incognitaand M. javanicathan the susceptibility pattern, the tomato 'Rutgers'. Other giloaccessions CNPH 070, CNPH 219,and CNPH 387 showed better or equivalent response thanthe resistant tomato 'Nemadoro' for M. enterolobii.4-the BER EG203 x S. scuticuminterspecific hybrid can be recommended as a rootstock for eggplant susceptible to M. incognita, as well the wild S. stramonifoliumvar. inerme species for M. enterolobii.

scholarly journals Resistance sources to root-knot nematode Meloidogyne enterolobii in Solanum species

2020 ◽  
pp. 303
Author(s):  
Jadir Borges Pinheiro ◽  
Giovani Olegario da Silva ◽  
Jhenef Gomes de Jesus ◽  
Danielle Biscaia ◽  
Raphael Augusto de Castro e Melo
Keyword(s):  
Solanum Species ◽  
Experimental Unit ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Second Stage ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Plant Grown ◽  
Reproduction Factor

The objective of this work was to prospect sources of resistance to root-knot nematode Meloidogyne enterolobii in Solanum species with potential to be used as rootstocks for cultivated Solanaceae. Nine accessions of Solanum sessiliflorum, 27 accessions of S. lycocarpum, 21 accessions of S. acanthodes, 22 accessions of S. scinericum and 26 accessions of S. scuticum for resistance to M. enterolobii. Rutgers and Nemadoro tomatoes were used as susceptible and resistant controls, respectively. The experiment was conducted in a greenhouse at Embrapa Vegetables, Brasília-DF, Brazil, in a completely randomized design with six replications. The experimental unit was a represented by a single plant grown in a plastic pot containing 3 L of substrate. 4000 eggs and eventual juveniles of second stage M. enterolobii were inoculated per pot. At 119 days after inoculation, gall index (Gi), egg mass index (EMI), number of eggs per root gram (NE) and reproduction factor (Fr) were evaluated. Data were subjected to analysis of variance and grouping of treatments by Scott-Knott. It was verified that S. acanthodes and S. Lycocarpum are species with high resistance to M. enterolobii, with accessions being classified identified as immune. S. scuticum also has great potential, as several resistant accessions were identified, although some accessions were quite susceptible; whereas for S. subinerme only 4 resistant accessions were identified, although all others presented a reproduction factor much lower than tomato cv. Nemadoro as control; and all evaluated S. sessiliflorum accessions were susceptible.

scholarly journals Do the stage of plant and initial population of root knot nematode influence on sweet pepper development?

O Biológico ◽  
2020 ◽  
Vol 82 (1) ◽  
pp. 1-10
Author(s):  
Samara A. de Oliveira ◽  
Juliana M. O Rosa ◽  
Juliana Eulálio ◽  
Claudio Marcelo G. de Oliveira
Keyword(s):  
Sweet Pepper ◽  
Initial Population ◽  
Root Knot Nematode ◽  
One Pot ◽  
Leaf Pair ◽  
Final Population ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Shoot Weight ◽  
Reproduction Factor

The aim of this study was to investigate the response of three different stages (one, three and five leaf pairs) of sweet pepper (Capsicum annuum cv. Orazio) seedlings to five inoculation levels of Meloidogyne enterolobii (zero (control), 300, 1000, 3000 and 10000) under greenhouse conditions. Each plant was cultivated in one pot filled with 3.8 L of substrate. The test was a completely randomized design with four replications. The plants were assessed 60 days after inoculation, plant shoot weight, final population of nematodes, and reproduction factor were measured. The results were fitted to Seinhorst model: Y = m + (1-m). ZPi –T. The results showed a tolerance limit (T) of 2,500 nematodes for plants with one and three leaf pair, and 8,500 nematodes for the five-leaf pair plant.

Influence of various feeding regimes on the performance of Archachatina marginata snail

2020 ◽  
Vol 45 (2) ◽  
pp. 209-214
Author(s):  
C. N. Okocha ◽  
D. N. Onunkwo
Keyword(s):  
Manihot Esculenta ◽  
Carica Papaya ◽  
Growth Parameters ◽  
Colocasia Esculenta ◽  
Small Scale ◽  
Randomized Design ◽  
Xanthosoma Sagittifolium ◽  
Feeding Regimes ◽  
Completely Randomized Design ◽  
Small Scale Farmers

This study was to evaluate the growth response of Archachatina marginata as influenced by four natural diets: Pawpaw (Carica papaya) cocoyam (Colocasia esculenta), cocoyam (Xanthosoma sagittifolium), and cassava (Manihot esculenta) leaves and growers mash (a compounded ration). One hundred and twenty (120) grower snails (Archachatina marginata) were purchased from a farm in Ibadan, Oyo State. Data were collected on the growth parameters (weight, length and circumference of shell of the snails arranged in a Completely Randomized Design and replicated three (3) times. The results showed that pawpaw (Carica papaya) recorded the highest mean in all the parameters measured and throughout the duration of the experiment. Cocoyam (Colocasia esculenta) leaf had a better mean than the Cassava (Manihot esculenta) leaves in terms of weight gained whereas the reverse is the case in terms of length and circumference of shell. Pawpaw (Carica papaya) is therefore, recommended to both local and small scale farmers in Abia State and in Nigeria for feeding their snails and cocoyam (Colocasia esculenta) leaves and growers mash as an alternate feed.

scholarly journals Evaluation of Antagonistic Plant Materials to Control Southern Root Knot Nematode in Tomato

2013 ◽  
Vol 14 ◽  
pp. 78-86
Author(s):  
Arun Kafle
Keyword(s):  
Avena Sativa ◽  
Nematode Population ◽  
Tagetes Patula ◽  
Root Knot Nematode ◽  
Plant Materials ◽  
Randomized Design ◽  
Final Count ◽  
Juvenile Population ◽  
Completely Randomized Design ◽  
Southern Root Knot Nematode

Marigold ((Tagetes patula), crotalaria (Crotalaria juncia), rapeseed plant (Brassica rapa) and oat (Avena sativa) as antagonistic plant and tomato (Lycopersicon esculentum), CL1104 variety as control, were evaluated to determine the effect on southern root knot nematode population in pot experiment at Tsukuba, Japan in year 2010. Completely Randomized Design (CRD) with three replications was used. Initial juvenile population was 30.0±2.8 per 20g soil. The juvenile nematode population was counted 63 days after transplanting and biomass of plants was incorporated in soil. Population was again counted 11 days and 13 day after biomass incorporation. At final count of Juvenile nematode population, it was found highest in tomato (66.6 /20g soil). Highest control was achieved in marigold (2.3 juvenile/20g soil) followed by crotalaria (3.0 juvenile/20g soil. Marigold and crotalaria followed by oat in rotation with tomato were observed as best antagonist plants to control Southern root knot nematode.

scholarly journals Grey Oyster Mushroom Biofarm for Small-Scale Entrepreneurship

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Desta Berhe Sbhatu ◽  
Haftom Baraki Abraha ◽  
Hiluf Tekle Fisseha
Keyword(s):  
Wheat Bran ◽  
Oyster Mushroom ◽  
Small Scale ◽  
Optimum Conditions ◽  
Home Based ◽  
Randomized Design ◽  
Polyethylene Bags ◽  
Completely Randomized Design ◽  
Aseptic Conditions ◽  
Growing Mass

This paper reports the productivity of a small-scale pilot biofarm of grey oyster mushroom (Pleurotus sajor-caju (Fr.) Sing.). The pilot was tested in Mekelle city (Ethiopia) in a brick-walled dark room. Growing structures were constructed by erecting three wood poles and fixing them with three wooden side bars at multiple locations to make a prism-shaped rack with multiple triangular open shelves, each capable of carrying one bag of spawned substrate. Mushroom substrates were prepared from maize stalk and wheat bran supplement. Pasteurized chopped maize stalk and wheat bran were mixed at the ratio of 10:0, 9:1, 8:2, and 7:3—yielding four treatments. Five kilograms of substrate was taken from each treatment and was mixed with one kilogram of gypsum to produce a growing mass. Each mass was spawned with 200 g of inoculum under aseptic conditions and put in polyethylene bags. The treatments were replicated thrice and the bags were put on the growth racks in completely randomized design. The growing room was maintained at optimum conditions. Maize stalk substrates supplemented with 10% and 20% of wheat bran have resulted in statistically comparable productivities but statistically significantly higher than those grown on nonsupplemented and highly supplemented maize stalk substrates (p ≤ 0.05). The ingenuity of the design and the convenience of the construction of the racks, the availability of the substrates, and the simplicity of the management and maintenance of the biofarm rendered the piloted design suitable for home-based and small- and medium-scale mushroom biofarm entrepreneurship.

scholarly journals Quantification of the damage caused by Meloidogyne enterolobii in okra

2019 ◽  
Vol 54 ◽  
Author(s):  
Edgard Henrique Costa Silva ◽  
Renato Silva Soares ◽  
Heloísa Oliveira Borges ◽  
Carolina Andrade Franco ◽  
Leila Trevisan Braz ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Abelmoschus Esculentus ◽  
Second Stage ◽  
Randomized Design ◽  
Meloidogyne Enterolobii ◽  
Completely Randomized Design ◽  
Suitable Measure ◽  
Reproduction Factor ◽  
Selection Of

Abstract: The objective of this work was to estimate the damage caused by Meloidogyne enterolobii in okra (Abelmoschus esculentus) and to verify the reliability of the reproduction factor as a suitable measure for selecting resistant okra genotypes. Increasing populations of M. enterolobii - 0, 500, 1,500, 3,000, 5,000, and 7,000 eggs and second-stage juveniles (J2) per plant, - were evaluated, in a completely randomized design. The pathogen showed a parasitism pattern similar to that of M. incognita, causing a significant decrease in morphological and agronomic traits. The pathogen reproduction factor should be used in the selection of okra genotypes for tolerance to M. enterolobii, in populations above 3,000 eggs or J2.

scholarly journals Effect of Cowpea Seed Beetle (Callosobruchus maculatus) Infestation on Selected Landraces of Bambara Groundnut During Storage

2021 ◽  
Vol 9 (2) ◽  
pp. 31-34
Author(s):  
Isma’ila Muhammad ◽  
Nasiru Abdullahi ◽  
Abdulmalik Bala Shu’aibu ◽  
Ibrahim Musa ◽  
Bawa Zakari Gambo ◽  
...  
Keyword(s):  
Storage Time ◽  
Callosobruchus Maculatus ◽  
Bambara Groundnut ◽  
State University ◽  
Sources Of Resistance ◽  
Improvement Program ◽  
Randomized Design ◽  
Percentage Weight ◽  
Cowpea Seed ◽  
Completely Randomized Design

The study was carried out to assess the effect of Callosobruchus maculatus infestation on selected Bambara groundnut (Vigna subterranean) landraces in Gombe during storage to determine the Landrace (s) that are less susceptible to infestation caused by C. maculatus during storage. The trial was done in the Botany laboratory of Gombe State University, Gombe. Seeds were collected from five different Landraces, 40 grams each of healthy and C. maculatus infestation free seeds were infested with two males and three females. Completely Randomized Design (CRD) with three replicates was used to assess the effects of C. maculatus infestation on the selected landraces of Bambara groundnut during storage. The results of the analysis of variance displayed significant differences (p≤ 0.05) among the parameters studied. It indicated that the Mottled and Cream landraces are relatively less susceptible and witness lower damage from the bruchids attack 14.6 (36%) and 17 (46%), whereas Red and Black landraces are more susceptible 24 (61 %) and 22 (57 %) (recorded higher damage from the C. maculatus in terms of percentage weight loss recorded). These results showed the existence of variation among the different Bambara groundnut landraces used due to the infestation of C. maculatus. It can be observed from this study that the use of infestation free landraces at storage time may perhaps be a worthwhile and auspicious factor for integrated pest management especially on bruchids in Bambara groundnuts. additionally, Mottle and Cream landraces might be employed as sources of resistance genes for the subsequent plant improvement program.

scholarly journals Development and Genetic Characterization of Peanut Advanced Backcross Lines That Incorporate Root-Knot Nematode Resistance From Arachis stenosperma

2022 ◽  
Vol 12 ◽  
Author(s):  
Carolina Ballén-Taborda ◽  
Ye Chu ◽  
Peggy Ozias-Akins ◽  
C. Corley Holbrook ◽  
Patricia Timper ◽  
...  
Keyword(s):  
Seed Size ◽  
Crop Improvement ◽  
Flower Color ◽  
Nematode Resistance ◽  
Wild Relative ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Phenotypic Data ◽  
Nematode Reproduction ◽  
Advanced Backcross

Crop wild species are increasingly important for crop improvement. Peanut (Arachis hypogaea L.) wild relatives comprise a diverse genetic pool that is being used to broaden its narrow genetic base. Peanut is an allotetraploid species extremely susceptible to peanut root-knot nematode (PRKN) Meloidogyne arenaria. Current resistant cultivars rely on a single introgression for PRKN resistance incorporated from the wild relative Arachis cardenasii, which could be overcome as a result of the emergence of virulent nematode populations. Therefore, new sources of resistance may be needed. Near-immunity has been found in the peanut wild relative Arachis stenosperma. The two loci controlling the resistance, present on chromosomes A02 and A09, have been validated in tetraploid lines and have been shown to reduce nematode reproduction by up to 98%. To incorporate these new resistance QTL into cultivated peanut, we used a marker-assisted backcrossing approach, using PRKN A. stenosperma-derived resistant lines as donor parents. Four cycles of backcrossing were completed, and SNP assays linked to the QTL were used for foreground selection. In each backcross generation seed weight, length, and width were measured, and based on a statistical analysis we observed that only one generation of backcrossing was required to recover the elite peanut’s seed size. A populating of 271 BC3F1 lines was genome-wide genotyped to characterize the introgressions across the genome. Phenotypic information for leaf spot incidence and domestication traits (seed size, fertility, plant architecture, and flower color) were recorded. Correlations between the wild introgressions in different chromosomes and the phenotypic data allowed us to identify candidate regions controlling these domestication traits. Finally, PRKN resistance was validated in BC3F3 lines. We observed that the QTL in A02 and/or large introgression in A09 are needed for resistance. This present work represents an important step toward the development of new high-yielding and nematode-resistant peanut cultivars.

scholarly journals Selection of interspecific Psidium spp. hybrids resistant to Meloidogyne enterolobii

2019 ◽  
Vol 41 ◽  
pp. e42702 ◽  
Author(s):  
Rodrigo Moreira Ribeiro ◽  
Vicente Martins Gomes ◽  
Alexandre Pio Viana ◽  
Ricardo Moreira Souza ◽  
Paulo Ricardo dos Santos
Keyword(s):  
Interspecific Hybrids ◽  
Genetic Parameters ◽  
Breeding Program ◽  
Economic Losses ◽  
Sources Of Resistance ◽  
Production Chain ◽  
Meloidogyne Enterolobii ◽  
Indirect Economic Losses ◽  
Selection Of

Since 2001, the disease known as 'guava decline', resulting from the interaction between the phytonematode Meloidogyne enterolobii and the fungus Fusarium solanie, has caused direct and indirect economic losses to the entire guava production chain. Given the lack of sources of resistance in guava genotypes, interspecific hybrids of Psidium spp. were obtained for resistance to the nematode M. enterolobii. To classify the level of resistance of the interspecific hybrids, we evaluated the plant classification methodologies proposed by Oostenbrink (1966) and Moura and Régis (1987). Estimates of genetic parameters were obtained using the REML/BLUP approach. Interspecific hybrids resistant to M. enterolobii were selected that can be used as rootstocks or in new crosses for the development of the guava breeding program.

scholarly journals Production of annatto seedlings on substrates based on decomposed babassu stem

2020 ◽  
Vol 14 ◽  
Author(s):  
Taciella Fernandes Silva ◽  
Klayton Antonio Do Lago Lopes ◽  
Igor Alves Da Silva ◽  
Janaiane Ferreira Dos Santos ◽  
Hosana Aguiar Freitas de Andrade ◽  
...  
Keyword(s):  
Seedling Growth ◽  
Economic Value ◽  
Small Scale ◽  
Bixa Orellana ◽  
Perennial Species ◽  
Important Species ◽  
Randomized Design ◽  
Entire Plant ◽  
Completely Randomized Design ◽  
Small Scale Farmers

Annatto (Bixa orellana L.) is an important species for several sectors, industrial, food, landscape, among others, which uses parts of the plant or the entire plant. It is an underresearched species, although it has significant economic value for small-scale farmers. The substrate is one of the sources that directly influence the production of a perennial species that need to present good characteristics to ensure a quality seedling. Therefore, the aim was to evaluate the babassu stem decomposed in the formulation of substrates for the production of annatto seedlings. The study was carried out a shading screen with 75% permeability to light. The experiments were conducted in a completely randomized design, with four replications. The treatments consisted of substrates based on babassu stem decomposed (BSD), in the following proportions: S0: 100% soil; S20: 20% BDS + 80% soil; S40: 40% BDS + 60% soil; S60: 60% BDS + 40% soil; S80: 80% BDS + 20% soil; S100: 100% BDS. At 90 days after sowing (DAS), an evaluation of seedling growth variables was performed. A difference was found between the procedures for all the variables analyzed. There was a difference between treatments for all variables analyzed. The substrate constituted of 60% babassu stem decomposed is the most appropriate for the production of taller annatto seedlings.

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