Development and Genetic Characterization of Peanut Advanced Backcross Lines That Incorporate Root-Knot Nematode Resistance From Arachis stenosperma

Crop wild species are increasingly important for crop improvement. Peanut (Arachis hypogaea L.) wild relatives comprise a diverse genetic pool that is being used to broaden its narrow genetic base. Peanut is an allotetraploid species extremely susceptible to peanut root-knot nematode (PRKN) Meloidogyne arenaria. Current resistant cultivars rely on a single introgression for PRKN resistance incorporated from the wild relative Arachis cardenasii, which could be overcome as a result of the emergence of virulent nematode populations. Therefore, new sources of resistance may be needed. Near-immunity has been found in the peanut wild relative Arachis stenosperma. The two loci controlling the resistance, present on chromosomes A02 and A09, have been validated in tetraploid lines and have been shown to reduce nematode reproduction by up to 98%. To incorporate these new resistance QTL into cultivated peanut, we used a marker-assisted backcrossing approach, using PRKN A. stenosperma-derived resistant lines as donor parents. Four cycles of backcrossing were completed, and SNP assays linked to the QTL were used for foreground selection. In each backcross generation seed weight, length, and width were measured, and based on a statistical analysis we observed that only one generation of backcrossing was required to recover the elite peanut’s seed size. A populating of 271 BC3F1 lines was genome-wide genotyped to characterize the introgressions across the genome. Phenotypic information for leaf spot incidence and domestication traits (seed size, fertility, plant architecture, and flower color) were recorded. Correlations between the wild introgressions in different chromosomes and the phenotypic data allowed us to identify candidate regions controlling these domestication traits. Finally, PRKN resistance was validated in BC3F3 lines. We observed that the QTL in A02 and/or large introgression in A09 are needed for resistance. This present work represents an important step toward the development of new high-yielding and nematode-resistant peanut cultivars.

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RESISTANCE OF WATERMELON (CITRULLUS LANATUS VAR. CITROIDES) GERMPLASM FOR RESISTANCE TO ROOT-KNOT NEMATODES

HortScience ◽

10.21273/hortsci.41.3.520a ◽

2006 ◽

Vol 41 (3) ◽

pp. 520A-520

Author(s):

J. A. Thies ◽

A. Levi

Keyword(s):

Citrullus Lanatus ◽

Nematode Resistance ◽

Root Knot Nematode ◽

Sources Of Resistance ◽

Root Knot Nematodes ◽

Nematode Reproduction ◽

Plant Introductions ◽

Race 1 ◽

Race 2 ◽

Moderate Resistance

Root-knot nematodes (Meloidogyne incognita, M. arenaria, and M. javanica) cause severe damage to watermelon and resistance has not been identified in any watermelon cultivar. In greenhouse tests, we evaluated 265 U.S. plant introductions (PIs) for nematode resistance (based on root galling and nematode reproduction), and identified 22 PIs of Citrullus lanatus var. citroides as moderately resistant to M. arenaria race 1. In subsequent tests, these 22 PIs exhibited low to moderate resistance to M. incognita race 3 and M. arenaria race 2. Three watermelon (C. lanatus var. lanatus) cultivars (Charleston Gray, Crimson Sweet, and Dixie Lee), three C. colocynthis PIs, and four C. lanatus var. citroides PIs, all previously shown to be susceptible to M. arenaria race 1, were susceptible to M. incognita race 3 and M. arenaria race 2. The C. lanatus var. citroides PIs that are most resistant to both M. incognita and M. arenaria should be useful sources of resistance for developing root-knot nematode resistant watermelon cultivars.

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Evaluation of eggplant and gilo genotypes and interspecific hybrids as to root-knot nematode resistance

Colloquium Agrariae ◽

10.5747/ca.2021.v17.n2.a427 ◽

2021 ◽

Vol 17 (2) ◽

pp. 30-38

Author(s):

Jadir Borges Pinheiro ◽

Giovani Olegario da Silva ◽

Jhenef Gomes de Jesus ◽

Danielle Biscaia ◽

Raphael Augusto de Castro e Melo

Keyword(s):

Interspecific Hybrids ◽

Nematode Resistance ◽

Small Scale ◽

Production Volume ◽

Root Knot Nematode ◽

Sources Of Resistance ◽

Randomized Design ◽

Meloidogyne Enterolobii ◽

Completely Randomized Design ◽

Significant Production

In Brazil, eggplant and gilo are important for the economy of small-scale farms located mainly in the southeast states and other regions, with a significant production volume year-round in the wholesale local markets. However, these species are very susceptible to root-knot nematodes, and there are few or almost none known sources of resistance. The objective of this studywas to prospect sources of resistance to root-knot nematodesin eggplant, scarlet eggplant (gilo), as well in interspecific hybrids between these species and with wildSolanumspecies, to be used as rootstocks. In the first experiment, in 2013,10 eggplant accessions, a hybrid between eggplant andgilo, and a Solanum stramonifoliumxeggplanthybrid, were evalu41atedfor theirreaction to Meloidogyne enterolobii. In the second, in 2016, 20 accessions of gilowere evaluated for their reaction to M. incognita, M. javanica,and M. enterolobii.. And in the third experiment,in 2017,one access and two experimental eggplant hybrids, and one Solanum scuticumx eggplant hybrid, were evaluated for their reaction to M. incognita, and M. enterolobii. All the trials were stablished inagreenhouse, and characters related to root infection were evaluated in a completely randomized design with six replications of one plant per pot, usinga 1.5 L pots filled with a mixedsubstrate inoculated with each nematode species.Itwas found thatall eggplant accessions were susceptible to M. incognitaand M. enterolobii, however, BER 3150 presented lower susceptibility to M. incognita. The gilogenotypes CNPH 056, CNPH 070, CNPH 220,and CNPH 363 shownbetter response to M. incognitaand M. javanicathan the susceptibility pattern, the tomato 'Rutgers'. Other giloaccessions CNPH 070, CNPH 219,and CNPH 387 showed better or equivalent response thanthe resistant tomato 'Nemadoro' for M. enterolobii.4-the BER EG203 x S. scuticuminterspecific hybrid can be recommended as a rootstock for eggplant susceptible to M. incognita, as well the wild S. stramonifoliumvar. inerme species for M. enterolobii.

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Root-Knot Nematode Resistance in Pearl Millet From West and East Africa

Plant Disease ◽

10.1094/pd-90-0339 ◽

2006 ◽

Vol 90 (3) ◽

pp. 339-344 ◽

Cited By ~ 2

Author(s):

P. Timper ◽

J. P. Wilson

Keyword(s):

East Africa ◽

Pearl Millet ◽

Egg Production ◽

Nematode Resistance ◽

Realized Heritability ◽

Root Knot Nematode ◽

Nematode Reproduction ◽

Diverse Origin ◽

Pearl Millet Cultivars ◽

Dominant Genes

Resistance to Meloidogyne incognita is important to provide stability to pearl millet production and to reduce nematode populations that can damage crops grown in rotation with pearl millet. The objectives of this study were to determine whether resistance to M. incognita exists in pearl millet from West and East Africa, and to determine if heterogeneity for resistance exists within selected cultivars. Resistance was assessed as nematode egg production per gram of root in greenhouse trials. Seventeen pearl millet cultivars of diverse origin were evaluated as bulk (S0) populations. All African cultivars expressed some level of resistance. P3Kollo was among the least resistant of the African cultivars, Zongo and Gwagwa were intermediate, and SoSat-C88 was among the most resistant. Thirty selfed (S1) progeny selections from SoSat-C88, Gwagwa, Zongo, and P3Kollo were evaluated for heterogeneity of resistance within cultivar. Reactions were verified in 13 S2 progeny of each of the four cultivars. In S1 evaluations, each of these cultivars was heterogeneous for resistance. Progeny reaction varied from highly resistant to highly susceptible. Patterns of apparent segregation of resistance varied among the four cultivars. Discreet resistant and susceptible phenotypes were identified in Zongo progeny, and it was estimated that two dominant genes for resistance segregated in this cultivar. Averaged across progenies, egg production on the four cultivars was less (P ≤ 0.001) than on the susceptible hybrid HGM-100, but was not different from resistant hybrid TifGrain 102. Reproduction of M. incognita on the S2 progeny tended to confirm the results from inoculations of S1 progeny. Heritability of nematode reproduction (standardized as the ratio of the value to HGM-100) determined by parent-offspring regression was 0.54. Realized heritability determined by divergent selection was 0.87.

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Identification and Evaluation of Additional Sources of Resistance to Peanut Root-knot Nematode in Arachis hypogaea L.1

Peanut Science ◽

10.3146/i0095-3679-23-2-5 ◽

1996 ◽

Vol 23 (2) ◽

pp. 91-94 ◽

Cited By ~ 7

Author(s):

C. Corley Holbrook ◽

James P. Noe ◽

Michael G. Stephenson ◽

William F. Anderson

Keyword(s):

Arachis Hypogaea ◽

Field Experiments ◽

Germplasm Collection ◽

Economic Losses ◽

Egg Mass ◽

Root Knot Nematode ◽

Sources Of Resistance ◽

Nematode Reproduction ◽

Plant Introductions ◽

Production Areas

Abstract The root-knot nematode (Meloidogyne arenaria race 1) causes significant economic losses throughout the peanut (Arachis hypogaea) production areas of the southern U.S. Chemicals for control of this pest are becoming increasingly limited, and there are no peanut cultivars with resistance. Seven moderately resistant plant introductions have been identified; however, less than 25% of the germplasm collection has been examined for resistance based on nematode reproduction. The objectives of this work were to examine an additional 1000 plant introductions for resistance to the peanut root-knot nematode and to compare the most resistant introductions to previously reported sources of resistance. Preliminary greenhouse screening trials were conducted to rate severity of root galling and amount of egg mass production. Seventeen accessions were selected based on a mean egg mass rating of less than or equal to three. These selections were reevaluated in additional greenhouse and field experiments to quantify levels of resistance and to directly compare these sources of resistance to those previously reported. Eight accessions had a significantly higher level of resistance (lower egg mass rating) than Florunner; however, none had a significantly higher level of resistance than those previously reported. Results of this study identified additional sources of resistance which may provide unique genes for resistance. In addition, two of these new sources of resistance (PI 298848 and PI 311265) exhibited significantly higher yield than those previously identified when grown in soil heavily infested with M. arenaria.

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Evaluation of Prunus Rootstocks for Root-lesion Nematode Resistance

HortScience ◽

10.21273/hortsci.31.6.1013 ◽

1996 ◽

Vol 31 (6) ◽

pp. 1013-1016 ◽

Cited By ~ 5

Author(s):

Eduard Alcañiz ◽

Jorge Pinochet ◽

Carolina Fernández ◽

Daniel Esmenjaud ◽

Antonio Felipe

Keyword(s):

Nematode Resistance ◽

Nematode Population ◽

Root Lesion Nematode ◽

Root Knot Nematode ◽

Nematode Reproduction ◽

Greenhouse Experiments ◽

Pratylenchus Vulnus ◽

Advanced Stages ◽

Lesion Nematode ◽

Moderately Resistant

Fourteen Prunus rootstocks were evaluated against mixtures of several isolates of the root-lesion nematode Pratylenchus vulnus Allen and Jensen in three greenhouse experiments. Most of the tested rootstocks are new releases or materials in advanced stages of selection that also have incorporated root-knot nematode resistance. The plums Torinel (Prunusdomestica L.) and Redglow (P. salicina Lindl. P. munsoniana Wight and Hedrick cv. Jewel) showed a moderately resistant response; their final nematode population levels were lower or slightly higher than inoculation levels. Low nematode reproduction also was found in the peach–almond hybrid G N No 22 [P. persica (L.) Batsch P. dulcis (Mill.) D.A. Webb] and the plum Bruce (P. salicina P. angustifolia Marsh.), and although these rootstocks did not perform as well as Torinel and Redglow, they also appear to be poor hosts for P. vulnus.

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Characterization of New Sources of Resistance in Cowpea to the Southern Root-knot Nematode

HortScience ◽

10.21273/hortsci.29.6.678 ◽

1994 ◽

Vol 29 (6) ◽

pp. 678-679 ◽

Cited By ~ 8

Author(s):

Richard L. Fery ◽

Philip D. Dukes ◽

Judy A. Thies

Keyword(s):

Egg Production ◽

Nematode Resistance ◽

Field Studies ◽

Egg Mass ◽

Root Knot Nematode ◽

Sources Of Resistance ◽

Dominant Type ◽

Plant Introductions ◽

Nematode Resistance Gene ◽

Southern Root Knot Nematode

A series of greenhouse and field studies was conducted over 9 years to characterize three new sources of resistance in cowpea [Vigna unguiculata (L.) Walp.] to the southern root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] and to determine if the resistances are conditioned by genes allelic to the Rk root-knot nematode resistance gene in `Mississippi Silver'. Three plant introductions (PI), PI 441917, PI 441920, and PI 468104, were evaluated for reaction to M. incognita in four greenhouse tests, and in every test each PI exhibited less galling, egg mass formation, or egg production than `Mississippi Silver'. F2 populations of the crosses between `Mississippi Silver' and each of the three resistant PIs were also evaluated for root-knot nematode resistance in a greenhouse test. None of the F2 populations segregated for resistance, indicating that PI 441917, PI 441920, and PI 468104 each has a gene conditioning resistance that is allelic to the Rk gene in `Mississippi Silver'. Our observations on the superior levels of resistances exhibited by PI 441917, PI 441920, and PI 468104 suggest that the allele at the Rk locus in these lines may not be the Rk allele, but one or more alleles that condition a superior, dominant-type resistance. The availability of additional dominant alleles would broaden the genetic base for root-knot nematode resistance in cowpea.

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Resistance in Peanut Cultivars and Breeding Lines to Three Root-Knot Nematode Species

Plant Disease ◽

10.1094/pdis-92-4-0631 ◽

2008 ◽

Vol 92 (4) ◽

pp. 631-638 ◽

Cited By ~ 4

Author(s):

W. B. Dong ◽

C. C. Holbrook ◽

P. Timper ◽

T. B. Brenneman ◽

Y. Chu ◽

...

Keyword(s):

Durable Resistance ◽

Nematode Species ◽

Nematode Resistance ◽

Root Knot Nematode ◽

Sources Of Resistance ◽

Breeding Lines ◽

Sequence Characterized Amplified Region ◽

Race 1 ◽

Meloidogyne Spp ◽

Peanut Cultivars

Three major species of root-knot nematode infect peanut: Meloidogyne arenaria race 1, M. hapla, and M. javanica race 3. Sources of resistance to all three nematodes are needed for developing novel peanut cultivars with broad resistance to Meloidogyne spp. Cultivars and breeding lines of peanut were evaluated for resistance to M. arenaria, M. hapla, and M. javanica in the greenhouse and in the laboratory. Twenty-six genotypes with some resistance to M. arenaria, M. javanica, or M. hapla were identified from 60 accessions based on average eggs per gram of root and gall index relative to a susceptible control. Among these, 14 genotypes were moderately to highly resistant to all three species, 5 genotypes were resistant to M. arenaria and M. javanica, 2 genotypes were resistant to M. javanica and M. hapla, 1 genotype was resistant M. arenaria alone, and 4 genotypes were resistant to M. hapla alone. Reproduction of M. arenaria on lines NR 0817, C724-19-11, and D108 was highly variable, indicating that these genotypes likely were heterogeneous for resistance. COAN, NemaTAM, C724-25-8, and the M. arenaria-resistant plants of C724-19-11 contained the dominant sequence-characterized amplified region marker (197/909) for nematode resistance. Results with the molecular markers indicate that the high resistance to M. arenaria in GP-NC WS 6 may be different from the resistance in COAN, NemaTAM, and C724-25-8. Resistance to M. arenaria was correlated with resistance to M. javanica in peanut, whereas resistance to M. hapla was not correlated with the resistance to either M. arenaria or M. javanica. The resistant selections should be valuable sources for pyramiding resistance genes to develop new cultivars with broad and durable resistance to Meloidogyne spp.

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034 Resistance to Root-knot Nematode in Vitis champinii

HortScience ◽

10.21273/hortsci.35.3.394a ◽

2000 ◽

Vol 35 (3) ◽

pp. 394A-394

Author(s):

Peter Cousins ◽

M. Andrew Walker

Keyword(s):

Root System ◽

Resistance Genes ◽

Meloidogyne Incognita ◽

Recessive Gene ◽

Nematode Resistance ◽

Progeny Testing ◽

Root Knot Nematode ◽

Nematode Reproduction ◽

Commercial Use

The grape Vitis champinii Planchon is one source of nematode resistance in grape rootstocks. Several selections valued for their resistance to the root-knot nematode (Meloidogyne incognita), a serious pest of grape production, are used as rootstocks and in rootstock variety development. However, V. champinii-based rootstock varieties are faulted for their excess vigor and susceptibility to other root pests. Root-knot nematode populations with the ability to damage important V. champinii-based rootstocks have been identified and may become more common. Other V. champinii accessions might be sources of nematode resistance genes with different specificities or might have more suitable horticultural characteristics than V. champinii varieties in commercial use. Nine V. champinii accessions from the National Clonal Germplasm Repository, Davis, Calif., and a V. champinii rootstock variety were screened for resistance to M. incognita. Resistance was assessed by counting eggs produced per root system. Eight of ten V. champinii accessions did not support nematode reproduction. Susceptible accessions supported lower nematode reproduction than susceptible V. vinifera control varieties. Progeny testing from crosses of resistant and susceptible accessions suggests that a dominant and a recessive gene may condition root-knot nematode resistance.

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Pearl Millet as a Rotation Crop for Peanut

Plant Health Progress ◽

10.1094/php-2007-0202-02-rs ◽

2007 ◽

Vol 8 (1) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

Patricia Timper ◽

Timothy B. Brenneman ◽

Jeffrey P. Wilson

Keyword(s):

Pearl Millet ◽

Pennisetum Glaucum ◽

Block Design ◽

Crop Improvement ◽

Nematode Resistance ◽

Population Increase ◽

Root Knot Nematode ◽

Improvement Programs ◽

Economic Analyses ◽

Rotation Crop

In the southeastern United States, there are limited options for crops that can be grown in rotation with peanut (Arachis hypogaea). Pearl millet (Pennisetum glaucum) has potential as a grain crop, and some hybrids have shown resistance to the peanut root-knot nematode (Meloidogyne arenaria), the primary nematode pest of peanut in this region. The objective of this study was to determine whether pearl millet reduces M. arenaria when planted in rotation with peanut. The experiment was arranged as a randomized, complete-block design with six replications. The rotations were peanut following either 2 years of corn, HGM-100 pearl millet, or TifGrain 102 pearl millet. There were two staggered sequences of each rotation so that a cycle was completed in 2004 and in 2005. Pearl millet did not increase either stem rot or Rhizoctonia limb rot in peanut. In both years, root galling from M. arenaria was lower on peanut following TifGrain 102 (4.6 on a scale of 0 to 10) and corn (4.9) than following HGM-100 (7.5). Peanut yields in 2004 were low and unaffected by the preceding rotation crop; however, in 2005, yields were greater in peanut following 2 years of TifGrain 102 (2320 kg/ha) and corn (2504 kg/ha) than following HGM-100 (1821 kg/ha). The lower yields following HGM-100 were likely due to greater populations of M. arenaria that had developed on the susceptible pearl millet hybrid. Economic analyses showed greater returns above variable costs from the grain crops than from the peanut crops. We conclude that the resistant pearl millet hybrid TifGrain 102 is as effective as corn in limiting population increase of M. arenaria and in enhancing peanut yield compared to the susceptible pearl millet hybrid, HGM-100. Additional research is needed to improve the profitability of pearl millet, and nematode resistance should be an important component of crop improvement programs. Accepted for publication 20 October 2006. Published 2 February 2007.

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Genetic Diversity and Population Structure of Serbian Barley (Hordeum vulgare L.) Collection during a 40-Year Long Breeding Period

Agronomy ◽

10.3390/agronomy11010118 ◽

2021 ◽

Vol 11 (1) ◽

pp. 118

Author(s):

Ljiljana Brbaklić ◽

Dragana Trkulja ◽

Sanja Mikić ◽

Milan Mirosavljević ◽

Vojislava Momčilović ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Hordeum Vulgare ◽

Crop Improvement ◽

Genetic Erosion ◽

Breeding Period ◽

Phenotypic Variance ◽

Hordeum Vulgare L ◽

Phenotypic Data ◽

Breeding Material

Determination of genetic diversity and population structure of breeding material is an important prerequisite for discovering novel and valuable alleles aimed at crop improvement. This study’s main objective was to characterize genetic diversity and population structure of a collection representing a 40-year long historical period of barley (Hordeum vulgare L.) breeding, using microsatellites, pedigree, and phenotypic data. The set of 90 barley genotypes was phenotyped during three growing seasons and genotyped with 338 polymorphic alleles. The indicators of genetic diversity showed differentiation changes throughout the breeding periods. The population structure discriminated the breeding material into three distinctive groups. The principal coordinate analysis grouped the genotypes according to their growth habit and row type. An analysis of phenotypic variance (ANOVA) showed that almost all investigated traits varied significantly between row types, seasons, and breeding periods. A positive effect on yield progress during the 40-year long breeding period could be partly attributed to breeding for shorter plants, which reduced lodging and thus provided higher yield stability. The breeding material revealed a considerable diversity level based on microsatellite and phenotypic data without a tendency of genetic erosion throughout the breeding history and implied dynamic changes in genetic backgrounds, providing a great gene pool suitable for further barley improvement.

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