Diagnostic value of Cyto-pick compared to cotton swab sampling for uterine cervical neoplasm screening in pregnant women.

Abstract Background Chorioamnionitis may cause serious perinatal and neonatal adverse outcomes, and group B streptococcus (GBS) is one of the most common bacteria isolated from human chorioamnionitis. The present study analyzed the impact of GBS infection and histological chorioamnionitis (HCA) on pregnancy outcomes and the diagnostic value of various biomarkers. Methods Pregnant women were grouped according to GBS infection and HCA detection. Perinatal and neonatal adverse outcomes were recorded with a follow-up period of 6 weeks. The white blood cell count (WBC), neutrophil ratio, and C-reactive protein (CRP) level from peripheral blood and soluble intercellular adhesion molecule-1 (sICAM-1), interleukin 8 (IL-8), and tumor necrosis factor α (TNF-α) levels from cord blood were assessed. Results A total of 371 pregnant women were included. Pregnant women with GBS infection or HCA had a higher risk of pathological jaundice and premature rupture of membranes and higher levels of sICAM-1, IL-8, and TNF-α in umbilical cord blood. Univariate and multivariate regression analysis revealed that sICMA-1, IL-8, TNF-α, WBC, and CRP were significantly related to an increased HCA risk. For all included pregnant women, TNF-α had the largest receiver operating characteristic (ROC) area (area: 0.841; 95% CI: 0.778–0.904) of the biomarkers analyzed. TNF-α still had the largest area under the ROC curve (area: 0.898; 95% CI: 0.814–0.982) for non-GBS-infected pregnant women, who also exhibited a higher neutrophil ratio (area: 0.815; 95% CI: 0.645–0.985) and WBC (area: 0.849; 95% CI: 0.72–0.978), but all biomarkers had lower value in the diagnosis of HCA in GBS-infected pregnant women. Conclusion GBS infection and HCA correlated with several perinatal and neonatal adverse outcomes. TNF-α in cord blood and WBCs in peripheral blood had diagnostic value for HCA in non-GBS-infected pregnant women but not GBS-infected pregnant women.

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Evaluation of free and total L-thyroxine in serum by a commercial procedure.

Clinical Chemistry ◽

10.1093/clinchem/27.1.149 ◽

1981 ◽

Vol 27 (1) ◽

pp. 149-152 ◽

Cited By ~ 3

Author(s):

M J Obregon ◽

A Kurtz ◽

R Ekins ◽

G Morreale de Escobar

Keyword(s):

Pregnant Women ◽

Plasma Proteins ◽

Diagnostic Value ◽

Free Thyroxine ◽

Total Thyroxine ◽

Dialysis Method ◽

Hypothyroid Patients ◽

Hyperthyroid Patients ◽

Normal Controls

Abstract We assessed a commercial kit (Corning Medical) for "free" and total thyroxine determination, results being compared to those obtained by the Ekins and Ellis dialysis method (free thyroxine) and the method of Weeke and Orskov (total thyroxine). The kit procedure permits determination of both free and total thyroxine within 4 to 5 h, and the combined results may disclose changes in binding to plasma proteins that would be missed if only free thyroxine were determined. With both free-thyroxine methods, the values distinguished hyperthyroid patients from normal controls and pregnant women with 100% accuracy, but there was some overlap between hypothyroid patients and controls. Absolute values with the kit procedure often exceed those obtained by dialysis, especially for hypothyroid patients and pregnant women. We conclude that the kit may be of as much diagnostic value as the dialysis method if the limitations regarding absolute values are kept in mind and the test is not used as a substitute for thyrotropin determinations in cases of suspected hypothyroidism.

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Diagnostic Value of An Abnormal Sr in Non-Pregnant Women: Evaluation of Positive Smear from the Surface of the Portio Obtained by the Cottonswab Method or by the Dry Wooden Ayre Spatula and the Relevance of Positive Smear from the Endocervix and/or from t

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.3109/00016349009006161 ◽

1990 ◽

Vol 69 (2) ◽

pp. 147-151

Author(s):

Lene Lundvall ◽

Lise Lotte Hajgaard ◽

Knud Hajgaard ◽

Finn Lundvall

Keyword(s):

Pregnant Women ◽

Diagnostic Value ◽

Positive Smear

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Uterine Cervical Neoplasm Diagnosed by Flexible Magnifying Endoscopy with Narrow Band Imaging

Diagnostics ◽

10.3390/diagnostics10110903 ◽

2020 ◽

Vol 10 (11) ◽

pp. 903

Author(s):

Hideki Kobara ◽

Kunihisa Uchita ◽

Noriya Uedo ◽

Noriko Matsuura ◽

Noriko Nishiyama ◽

...

Keyword(s):

Cervical Cancer ◽

Pap Smear ◽

Narrow Band ◽

Narrow Band Imaging ◽

Early Stage ◽

Magnifying Endoscopy ◽

Diagnostic Tools ◽

Gastrointestinal Neoplasms ◽

Cervical Neoplasm ◽

Uterine Cervical Neoplasm

When detected early, uterine cervical cancer is one of the most successfully treatable forms of cancer. The diagnostic accuracy of the standard method, the Pap smear test followed by colposcopy, remains unsatisfactory. To improve detection of early-stage cervical cancer, new diagnostic tools for uterine cervical intraepithelial neoplasm (CIN) need to be developed. Magnifying endoscopy with narrow- band imaging (ME-NBI), which allows the visualization of the micro-structure as well as micro-vascularity of the mucosal surface, has excellent diagnostic ability for early gastrointestinal neoplasms. In our previous investigation, ME-NBI was efficacious for diagnosis of CIN. We herein report two notable cases of CIN3 that were diagnosed by ME-NBI that were not detected by colposcopy. These cases illustrate the usefulness of ME-NBI for diagnosis of early-stage uterine cervical neoplasms.

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Diagnostic accuracy and value of chromosomal microarray analysis for chromosomal abnormalities in prenatal detection: a prospective clinical study

10.21203/rs.3.rs-59569/v1 ◽

2020 ◽

Author(s):

Hailong Huang ◽

Yan Wang ◽

Min Zhang ◽

Na Lin ◽

Gang An ◽

...

Keyword(s):

Pregnant Women ◽

Microarray Analysis ◽

Operating Characteristic ◽

Chromosomal Abnormalities ◽

Area Under The Curve ◽

Diagnostic Value ◽

Chromosomal Microarray ◽

Prospective Clinical Study ◽

Chromosomal Microarray Analysis ◽

Success Rates

Abstract Background Chromosomal microarray analysis (CMA) has emerged as a primary diagnostic tool for the evaluation of developmental delay and structural malformations in children. The aim of this study was to compare the accuracy and diagnostic value of CMA and karyotyping on chromosomal abnormalities in Fujian province of South China. Methods In the study, 410 samples were obtained from pregnant women between March 2015 and December 2016, including 3 villus (0.73%, 3/410), 296 amniotic fluid (72.20%, 296/410), and 111 umbilical cord blood (27.07%, 111/410). Each sample was screening for chromosomal abnormalities by both using CMA and karyotyping. Results The success rates of CMA and karyotyping were 100% (410/410) and 99.27% (407/410), respectively. 61 (14.88%, 61/410) samples were presented with chromosomal abnormalities using CMA, whereas 47 (11.46%, 47/410) samples were shown with chromosomal abnormalities using karyotyping. 31 (7.56%, 31/410) samples with normal karyotypes were found to have chromosomal abnormalities using CMA. Receiver operating characteristic (ROC) analysis showed that the area under the curve (AUC) of CMA on the diagnosis of chromosomal abnormalities was 0.93, with 90.68% sensitivity and 94.40% specificity. The AUC of karyotyping on the diagnosis of chromosomal abnormalities was 0.90, with 87.56% sensitivity and 91.22% specificity. Conclusions Our data demonstrated that CMA has a better diagnostic value for screening chromosomal abnormalities, especially for pregnant women with normal karyotypes.

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A role of comprehensive cervix assessment in the first trimester of pregnancy for predicting preterm delivery

Obstetrics Gynecology and Reproduction ◽

10.17749/2313-7347/ob.gyn.rep.2020.094 ◽

2020 ◽

Vol 14 (2) ◽

pp. 174-181

Author(s):

V. G. Volkov ◽

O. V. Chursina

Keyword(s):

Pregnant Women ◽

First Trimester ◽

Diagnostic Value ◽

Singleton Pregnancy ◽

Group 4 ◽

First Trimester Of Pregnancy ◽

Group 3 ◽

Group 2 ◽

Delivery Term ◽

Group 1

Aim: to improve efficacy of predicting preterm labor in the first trimester of pregnancy by combining diverse parameters of cervical ultrasound examination.Materials and methods. A prospective cohort study of 1517 women with uncomplicated pregnancy was performed. Inclusion criteria were: females underwent cervicometry at 11–14 weeks of gestation, singleton pregnancy, revealing no complaints at the onset of examination. All women were subdivided into four groups: Group 1 – 27 pregnant women with shortened cervix (less than 30 mm); Group 2 – 24 pregnant women without cervical gland area (СGA); Group 3 – 30 pregnant women with two risk factors (shortened cervix less than 30 mm and lacked СGA); Group 4 (control) consisted of 1436 pregnant women with cervix length exceeding 30 mm and presence of СGA.Results. Average delivery term in Group 1 was 35.7 weeks (95 % CI = 34.7–36.8), in Group 2 – 34.7 weeks (95 % CI = 33.59–35.0), in Group 3 – 33.23 weeks (95 % CI = 31.6–34.8), in Group 4 (control) – 38.11 weeks (95 % CI = 38.06–38.17). A significant moderate correlation (Rxy = 0.534) between shortened cervix, absence of СGA and delivery term was found (p < 0.001). A regression model consisting of cervical length and presence of СGA was simulated based upon 50.8 % factors underlying probability of preterm birth, revealing 42.6 % sensitivity, 99.1 % specificity, and 96.6 % overall diagnostic value. The area under the ROC curve was 0.902 ± 0.022 (95 % CI = 0.860–0.945). The data obtained reflect diverse biochemical changes such as collagen decomposition, altered glucosamine level and fluid amount within cervical tissues. Such processes result in shortened, softened and expanded (matured) cervix. Uncovering markers for preterm cervical maturation underlies a logical strategy to predict miscarriage.Conclusion. Untrasound cervical measurement in the first trimester of pregnancy allows for revealing valuable miscarriage predictors (shortened cervix and absence of GI). Risk assessment by combining diverse ultrasound cervix parameters would allow to improve overall predictive efficacy.

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The diagnostic Value of Vascular Endothelial Growth Factor in Women with Growth-Restricted Fetuses

Doctor Ru ◽

10.31550/1727-2378-2020-19-8-44-48 ◽

2020 ◽

Vol 19 (8) ◽

pp. 44-48

Author(s):

I.F. Fatkullin ◽

◽

N.R. Akhmadeev ◽

E.V. Ulyanina ◽

L.Kh. Islamova ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Pregnant Women ◽

Fetal Growth ◽

Serum Levels ◽

Diagnostic Value ◽

Vascular Endothelial ◽

Group I ◽

Low Weight ◽

Endothelial Growth Factor

Study Objective: To assess delivery outcomes in women with slow fetal growth (ICD-10 code P05) and study serum levels of vascular endothelial growth factor (VEGF) and its diagnostic value in patients with growth-restricted fetuses that have constitutional low weight, in order to choose an approach to pregnancy management. Study Design: This was a prospective and retrospective comparative study. Materials and Methods: The medical charts of 782 women who had given birth to low-weight babies in 2013 and 2014 were reviewed retrospectively. The prospective part of the study involved measuring serum levels of VEGF in 150 pregnant women: 50 women with growthrestricted fetuses (Group I), 50 women with low-weight fetuses without hemodynamic disruptions or placental abnormalities (Group II), and 50 women with healthy pregnancies (Group III). Based on the babies’ gestational term at birth, the women in each group were divided into two subgroups: between 22 weeks and 0 days and 31 weeks and 6 days (subgroup 1) and ≥32 weeks and 0 days (subgroup 2). The data obtained were processed using descriptive statistics and compared with data obtained through other fetal assessments. Study Results: The pregnant women with growth-restricted fetuses had higher VEGF levels by a statistically significant margin (p<0.00001) than the women in groups II and III: median levels were 310 (Q1–Q3: 270–508), 33 (Q1–Q3: 31–38), and 36 (Q1–Q3: 32–40) pg/mL, respectively, in subgroups 1 and 174 (Q1–Q3: 100–214), 78 (Q1–Q3: 73–86), and 82 (Q1–Q3: 78–88) pg/mL, respectively, in subgroups 2. VEGF levels ≥100 pg/mL were associated with fetal growth restriction (FGR) (p = 0.0001) and levels ≥200 pg/mL with a high risk of antenatal fetal death (p = 0.026) or early neonatal death (p = 0.03). Conclusion: For women with growth-restricted fetuses, VEGF serum levels ≥200 pg/mL are an additional risk factor for unfavorable perinatal outcomes, which helps to optimize obstetrical management for these patients. Keywords: low-weight fetus, fetal growth retardation, vascular endothelial growth factor.

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Diagnostic Value of Rapid Biophysical Profile in Comparison to Biophysical Profile in Pregnant Women with Insulin-dependent Diabetes

Journal of Family & Reproductive Health ◽

10.18502/jfrh.v13i4.2648 ◽

2020 ◽

Author(s):

Nasrin Soufizadeh ◽

Fariba Farhadifar ◽

Saghar Tamri ◽

Sara Behafarid ◽

Karim Sharifi ◽

...

Keyword(s):

Pregnant Women ◽

Gestational Age ◽

Roc Curve ◽

Diagnostic Value ◽

Insulin Dependent Diabetes ◽

Care Process ◽

Predictive Values ◽

Biophysical Profile ◽

Insulin Dependent ◽

Sensitivity Specificity

Objective: Having a rapid and low cost diagnostic approach in assessment of fetal wellbeing is an important goal for prenatal care process. The aim of this study was to determine the diagnostic value of rapid biophysical profile (rBPP) in comparison to biophysical profile (BPP). Materials and methods: In this study 142 pregnant women with insulin-dependent diabetes referred to Besat Hospital (Sanandaj, Iran) were evaluated in terms of fetal health. Age, gestational age and non-stress test (NST) data of patients were collected. The fetuses were evaluated using the standard BPP and selected rBPP methods. Sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) were calculated. The receiver operating characteristic (ROC) curve was plotted. The data were analyzed in Stata 14 software, using appropriate statistical analyses. Results: The mean ± standard deviation (SD) of maternal age and gestational age of the studied subjects were 30.6 ± 6.3 and 35.6 ± 1.5 weeks, respectively. The frequency of normal cases were 126 (88.7%) in the BPP method and 121 (85.2%) in the rBPP method. The results showed that sensitivity, specificity, PPV and NPV of rBPP in this study were 56.2%, 90.5%, 42.8% and 94.2%, respectively. The area under the ROC curve was 73.3%. Pearson Test showed a significant correlation between scores obtained through BPP and rBPP methods (p < 0.001). Conclusion: Considering the high profile of the sensitivity and PPV of the RBPP method compared to BPP, rBPP method has a better capacity to discriminate non-distressed fetuses from distress-exposed fetuses. It can also be used as a quick and easy method in crowded centers with limited evaluation tests, where not much skill is needed.

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Diagnostic significance of the Wassermann reaction in obstetrics

Kazan medical journal ◽

10.17816/kazmj76540 ◽

2021 ◽

Vol 20 (4) ◽

pp. 431-432

Author(s):

V. Gruzdev

Keyword(s):

Positive Result ◽

Pregnant Women ◽

Diagnostic Value ◽

The Other ◽

Diagnostic Significance ◽

Early Stages ◽

The One ◽

Wassermann Reaction

According to G. Richter's observations (report in Zentr. F. Gyn., 1923, No. 35), in assessing this value, a strict distinction must be made between pregnant women in the early stages of pregnancy, on the one hand, and pregnant women in the later stages, and also by women in labor and parturient women shortly after childbirth, on the other: while in the former a positive RW indicates the presence of lues, in the latter it cannot be given the same diagnostic value to a positive result.

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