Effect of High Intensity Interval Training with Flaxseed on Interleukin-1 Beta and Lipocalin-2 Gene Expressions in the Heart Tissue of Rats

Abstract Objectives: We investigated the effects of 12 weeks of high-intensity interval training (HIIT) on selected circulating adipokines and other cardiovascular diseases (CVD) risks factors in males with obesity. Methods: Thirty males with obesity were randomly assigned to HIIT and control groups. The HIIT group participated in a prescribed exercise program for 12 weeks, three times per week. Blood lipids, insulin resistance, and select serum adipokines were assessed before and after 12 weeks of the intervention period. Results: HIIT improved body composition and lipid profiles (p<0.05) as well as decreased fasting insulin levels (p=0.001) and HOMA-IR (p=0.002) levels. Furthermore, HIIT increased levels of lipocalin-2 (lcn2) (p=0.002) while decreasing omentin-1 levels (p=0.001) in males with obesity. Changes in lcn2 and omentin-1 concentrations correlated with the changes in risk factors in the HIIT group (p<0.05). Conclusions: The results indicate that 12 weeks of supervised HIIT exercise significantly improves both circulating concentrations of lcn2 and omentin-1, two recently described adipokines, and markers of CVD risk in males with obesity. Further research is necessary to understand the molecular mechanisms involved with these changes.

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High-Intensity Interval Training Improves Lipocalin-2 and Omentin-1 Levels in Men with Obesity

International Journal of Sports Medicine ◽

10.1055/a-1560-5401 ◽

2021 ◽

Author(s):

Sirvan Atashak ◽

Stephen R. Stannard ◽

Ali Daraei ◽

Mohammad Soltani ◽

Ayoub Saeidi ◽

...

Keyword(s):

Cardiovascular Diseases ◽

High Intensity ◽

Molecular Mechanisms ◽

Interval Training ◽

Control Group ◽

Lipocalin 2 ◽

High Intensity Interval Training ◽

Before And After ◽

High Intensity Interval ◽

And Control

We investigated the effects of 12 weeks of high-intensity interval training (HIIT) on selected circulating adipokines and other cardiovascular diseases risks factors in men with obesity. Thirty men with obesity (age: 24.96 ± 3.11 year, BMI: 30.92 ± 1.04 kg/m2) were randomly assigned to HIIT and control groups. The HIIT group participated in a 12-week HIIT program (5×2 min interval bout at an intensity of 85-95% HRmax interspersed by 1 min passive recovery, three times per week), while the control group maintained their usual lifestyles. Blood lipids, insulin resistance, and select serum adipokines were assessed before and after 12 weeks of the intervention period. HIIT improved body composition and lipid profiles (p<0.05) and also decreased fasting insulin levels (p=0.001) and HOMA-IR (p=0.002) levels. Furthermore, HIIT increased levels of lipocalin-2 (p=0.002) while decreasing omentin-1 levels (p=0.001) in men with obesity. Changes in lcn2 and omentin-1 concentrations correlated with the changes in risk factors in the HIIT group (p<0.05). The results indicate that 12 weeks of supervised HIIT significantly improves both circulating concentrations of lcn2 and omentin-1, two recently described adipokines, and risk markers of cardiovascular diseases in men with obesity. Further research is necessary to understand the molecular mechanisms involved with these changes.

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The Effect of High Intensity Interval Training and Herb Supplement on Heart IGF-1 Concentration in Male Rat

International journal of basic science in medicine ◽

10.15171/ijbsm.2018.03 ◽

2018 ◽

Vol 3 (1) ◽

pp. 13-17

Author(s):

Mohamad Ali Pirani ◽

Maghsoud Peeri ◽

Mohamad Ali Azarbayjani

Keyword(s):

High Intensity ◽

Training Session ◽

Interval Training ◽

Heart Tissue ◽

Flaxseed Oil ◽

Male Rats ◽

Male Rat ◽

High Intensity Interval Training ◽

Training Groups ◽

High Intensity Interval

Abstract Objectives: The objective of the present study was to determine the effect of 10 weeks of high intensity interval training and flaxseed oil supplement on heart IGF-1 concentration in male rats. Materials and methods: 20 adult male Wistar rats were randomly divided into saline, saline–training, supplementation, and supplementation–training groups. The training groups performed training (10 weeks, five sessions per week, 90–95% VO2 max) on a rodent treadmill. The supplementation groups also received flaxseed oil supplement (30 mg/kg). The rats were sacrificed five days after the last training session. The heart tissue was collected, and sent to the laboratory for evaluation. Results: Training increased the concentration of the heart IGF-1 (P=0.01). The concentration of heart IGF-1 was higher in the flaxseed oil-supplemented groups than the saline-treated groups. (P=0.003). The interaction between training and supplementation also led to a increase in heart IGF-1 concentration (P=0.001). Conclusion: The increase of heart IGF-1 after training and consumption suggest that training and flaxseed oil can help to improve cardiac function.

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The Effect of Six Weeks of High Intensity Interval Training on eNOS and PGC-1α Gene Expression in the Heart Tissue of Male Obese Rats

Jundishapur Journal of Health Sciences ◽

10.5812/jjhs.100280 ◽

2020 ◽

Vol 12 (2) ◽

Author(s):

Behnoush Ghadery ◽

Farshad Ghazalian ◽

Seyed Ali Hosseini ◽

Hossein Abed Natanzy ◽

Alireza Shamsoddini

Keyword(s):

Gene Expression ◽

High Intensity ◽

Interval Training ◽

Heart Tissue ◽

Male Rats ◽

High Intensity Interval Training ◽

Expression Levels ◽

Obese Rats ◽

High Intensity Interval ◽

Gene Expression Levels

Background: Nowadays, obesity can affect heart function and induced atherosclerosis, high blood pressure, and heart arrhythmia, which has become a major problem for global health. Objectives: The present study aimed to review the effect of six weeks of high-intensity interval training (HIIT) on gene expression levels of PGC-1α and eNOS in the heart tissue of obese male rats. Methods: In this experimental study, 14 high-fat diet (HFD)-induced obese rats were divided into two groups of control and training. Also, 7 rats were placed in a non-obese control group to investigate the effects of obesity on research variables. During six weeks, rats in the training group performed HIIT three days per week. After six weeks, all rats were sacrificed, and their heart tissue was removed to measure the PGC-1α and eNOS gene expression. We used one-way ANOVA with Tukey’s post hoc tests (P ≤ 0.05) for statistical analysis. Results: HFD significantly decreased PGC-1α (P = 0.04) and eNOS (P = 0.001) gene expression, but HIIT significantly increased PGC-1α (P = 001) and eNOS (P = 0.001) gene expression. Conclusions: HIIT seems to improve cardiac gene expression levels of PGC-1α and eNOS of male obese rats.

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Treating fructose-induced metabolic changes in mice with high-intensity interval training: insights in the liver, white adipose tissue, and skeletal muscle

Journal of Applied Physiology ◽

10.1152/japplphysiol.00154.2017 ◽

2017 ◽

Vol 123 (4) ◽

pp. 699-709 ◽

Cited By ~ 6

Author(s):

Victor F. Motta ◽

Thereza L. Bargut ◽

Marcia B. Aguila ◽

Carlos A. Mandarim-de-Lacerda

Keyword(s):

Skeletal Muscle ◽

Adipose Tissue ◽

White Adipose Tissue ◽

High Intensity ◽

Interval Training ◽

Peroxisome Proliferator ◽

High Intensity Interval Training ◽

Gene Expressions ◽

Peroxisome Proliferator Activated Receptor ◽

High Intensity Interval

Fructose-rich caloric sweeteners induce adverse changes in the metabolism of humans. The study evaluated the effects of high-intensity interval training (HIIT) on a fructose feeding model, focusing on the liver, white adipose tissue (WAT), skeletal muscle, and their interplay. Male C57BL/6 mice were fed for 18 wk one of the following diets: control (C; 5% of total energy from fructose) or fructose (F; 55% of total energy from fructose). In the 10th week, for an additional 8-wk period, the groups were divided into nontrained (NT) or HIIT groups, totaling four groups: C-NT, C-HIIT, F-NT, and F-HIIT. At the end of the experiment, fructose consumption in the F-NT group led to a high systolic blood pressure, high plasma triglycerides, insulin resistance with glucose intolerance, and lower insulin sensitivity. We also observed liver steatosis, adipocyte hypertrophy, and diminished gene expressions of peroxisome proliferator-activated receptor-γ coactivator 1-α and fibronectin type III domain containing 5 (FNDC5; irisin) in this F-NT group. These results were accompanied by decreased gene expressions of nuclear respiratory factor 1 and mitochondrial transcription factor A (markers of mitochondrial biogenesis), and peroxisome proliferator-activated receptor-α and carnitine palmitoyltransferase 1 (markers of β-oxidation). HIIT improved all of these data in the C-HIIT and F-HIIT groups. In conclusion, in mice fed a fructose diet, HIIT improved body mass, blood pressure, glucose metabolism, and plasma triglycerides. Liver, WAT, and skeletal muscle were positively modulated by HIIT, indicating HIIT as a coadjutant treatment for diseases affecting these tissues. NEW & NOTEWORTHY We investigated the effects of high-intensity interval training (HIIT) in mice fed a fructose-rich diet and the resulting severe negative effect on the liver, white adipose tissue (WAT), and skeletal muscle, which reduced the expression of fibronectin type III domain containing 5 (FNDC5, irisin) and PGC1α and, consequently, affected markers of mitochondrial biogenesis and β-oxidation. Because HIIT may block these adverse effects in all of these three tissues, it might be suggested that it functions as a coadjutant treatment in combatting the alterations caused by high-fructose intake.

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