Camellia sinensis extract inhibits in vitro pancreatic lipase and has preventive effect on obesity in female rat fed a high-fat diet

Abstract Background Obesity, reached epidemic proportions globally is often associated with life threatening comorbidities. The unavailability of safe and effective long term medications for obesity in modern pharmacotherapy forces the scientific community to explore the potential of Ayurvedic traditional healers as they are considered safe and effective. Objective To explore the anti-obesity potential of aqueous extract of aerial parts of Achyranthes aspera L. (AEAA), a traditional healer in high fat diet (HFD) induced obese rats. Methods AEAA was prepared and subjected to in-vitro pancreatic lipase inhibition assay and in-vivo anti-obesity studies. For in-vivo studies, HFD fed obese prone Wistar albino rats were divided into five experimental groups (Group II to VI): animals fed with standard pellet chow served as normal control (Group I) while, animals continued with HFD alone served as obese control (Group II); Group III, IV and V were administered AEAA at a dose of 100, 200 and 300 mg/kg b.w. respectively along with HFD; and animals administered orlistat (30 mg/kg bw) along with HFD served as standard control (Group VI). All the drugs were administered orally once a day for a period of 60 days. At the end of the experimental period various physical, biochemical and histopathological observations were made. Results In-vitro studies showed AEAA partially but not significantly inhibited the activity of pancreatic lipase. Data of in-vivo studies revealed, significant reduction in body weights, fat pad weights and organ weights upon AEAA treatment. Elevated levels of glucose, insulin, leptin, lipid profiles and antioxidant status were also brought back to normal. Conclusion The obtained results clearly suggested that AEAA possess pronounced anti-obesity potential.

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Walnut Polyphenols Inhibit Pancreatic Lipase Activity in Vitro and Have Hypolipidemic Effect on High-Fat Diet-Induced Obese Mice

Journal of Food and Nutrition Research ◽

10.12691/jfnr-2-10-16 ◽

2014 ◽

Vol 2 (10) ◽

pp. 757-763 ◽

Cited By ~ 7

Author(s):

Dandan Shi ◽

Chaoyin Chen ◽

Shenglan Zhao ◽

Feng Ge ◽

Diqiu Liu ◽

...

Keyword(s):

Pancreatic Lipase ◽

Lipase Activity ◽

High Fat Diet ◽

Hypolipidemic Effect ◽

Obese Mice ◽

High Fat

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Anti-obesity effects of instant fermented teas in vitro and in mice with high-fat-diet-induced obesity

Food & Function ◽

10.1039/c9fo00162j ◽

2019 ◽

Vol 10 (6) ◽

pp. 3502-3513 ◽

Cited By ~ 4

Author(s):

Yue Sun ◽

Yuwan Wang ◽

Pengpeng Song ◽

Haisong Wang ◽

Na Xu ◽

...

Keyword(s):

Pancreatic Lipase ◽

High Fat Diet ◽

Inhibitory Effect ◽

High Fat ◽

Related Factors ◽

Diet Induced Obesity

IDT exerted better anti-obesity effects than PET in HFD-fed mice, as evidenced by reduced BW and regulated obesity-related factors. IDT also showed inhibitory effect on pancreatic lipase in vitro.

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Ethylacetate fraction of Anthocleista vogelii Planch demonstrates antiobesity activities in preclinical models

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i3.15 ◽

2021 ◽

Vol 18 (3) ◽

pp. 547-554

Author(s):

Gabriel O. Anyanwu ◽

Hafiz Misbah ud Din Qamar ◽

Jamshed Iqbal ◽

Syeda A. Ejaz ◽

Eusebius C. Onyeneke ◽

...

Keyword(s):

Mass Spectrometry ◽

Body Weight ◽

Pancreatic Lipase ◽

High Fat Diet ◽

Herbal Remedy ◽

Ionization Mass ◽

Sprague Dawley ◽

High Fat ◽

Leptin Sensitivity

Purpose: To assess the anti-obesity effect of liquid chromatography mass spectrometry (LCMS) profiled ethylacetate fraction (EF) of Anthocleista vogelii Planch on pancreatic lipase activity in vitro, and on obesity-related hormones in high-fat diet (HFD)-induced obese rats. Methods: Chromatographic analysis of EF to identify bioactive compounds was performed using LCMS electrospray ionization mass spectrometry (ESI-MS) positive mode. Thirty Sprague–Dawley rats were divided into 5 groups (n = 6). Group 1 was fed normal pellet diet, while groups 2 - 5 were fed high-fat diet (HFD) for 14 weeks. The rats were treated for 4 weeks from week 10 with 125 mg/kg of EF (group 3), 250 mg/kg of EF (group 4) or 100 mg/kg of orlistat (group 5). Results: Seven alkaloids were identified in EF, namely, 10-hydroxycamtothecin, moschamindole, camptothecin, moschamine, N6-cis-p-coumaroylserotonin, sinomenine and desacetylcolchicine. The EF of A. vogelii exhibited inhibitory activity against pancreatic lipase with half-maximal inhibitory concentration (IC50) of 8.76 ± 0.110 µg/mL. Rats treated with EF (125 and 250 mg/kg) of A. vogelii showed significantly (p < 0.05) decreased feed intake, body weight, leptin and insulin, when compared to HFD controls. Cortisol, serotonin and noradrenaline were significantly (p < 0.05) increased, but changes in thyroid hormones levels in EF-treated rats were not significant (p > 0.05) when compared to HFD controls. Conclusion: The EF of A. vogelii demonstrate anti-obesity activities by inhibiting pancreatic lipase, elevating serotonin and noradrenaline, and increasing leptin sensitivity, leading consequently to decreased body weight of rats. However, the clinical use of EF of A. vogelii as an antiobesity herbal remedy requires further studies on its mechanisms of action.

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Faculty Opinions recommendation of Chronic high-fat diet in fathers programs β-cell dysfunction in female rat offspring.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5716956.8455054 ◽

2011 ◽

Author(s):

Sandra Davidge ◽

Stephane Bourque

Keyword(s):

High Fat Diet ◽

Female Rat ◽

High Fat ◽

Β Cell ◽

Cell Dysfunction ◽

Rat Offspring

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Faculty Opinions recommendation of Chronic high-fat diet in fathers programs β-cell dysfunction in female rat offspring.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5716956.5697054 ◽

2010 ◽

Author(s):

Mehboob Hussain

Keyword(s):

High Fat Diet ◽

Female Rat ◽

High Fat ◽

Β Cell ◽

Cell Dysfunction ◽

Rat Offspring

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High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer’s Disease Signatures

International Journal of Molecular Sciences ◽

10.3390/ijms22073746 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3746

Author(s):

Ilaria Zuliani ◽

Chiara Lanzillotta ◽

Antonella Tramutola ◽

Eugenio Barone ◽

Marzia Perluigi ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Insulin Resistance ◽

Alzheimer's Disease ◽

High Fat Diet ◽

Mitochondrial Activity ◽

High Fat ◽

Hexosamine Biosynthetic Pathway ◽

Neurodegenerative Process ◽

The Brain

The disturbance of protein O-GlcNAcylation is emerging as a possible link between altered brain metabolism and the progression of neurodegeneration. As observed in brains with Alzheimer’s disease (AD), flaws of the cerebral glucose uptake translate into reduced protein O-GlcNAcylation, which promote the formation of pathological hallmarks. A high-fat diet (HFD) is known to foster metabolic dysregulation and insulin resistance in the brain and such effects have been associated with the reduction of cognitive performances. Remarkably, a significant role in HFD-related cognitive decline might be played by aberrant protein O-GlcNAcylation by triggering the development of AD signature and mitochondrial impairment. Our data support the impairment of total protein O-GlcNAcylation profile both in the brain of mice subjected to a 6-week high-fat-diet (HFD) and in our in vitro transposition on SH-SY5Y cells. The reduction of protein O-GlcNAcylation was associated with the development of insulin resistance, induced by overfeeding (i.e., defective insulin signaling and reduced mitochondrial activity), which promoted the dysregulation of the hexosamine biosynthetic pathway (HBP) flux, through the AMPK-driven reduction of GFAT1 activation. Further, we observed that a HFD induced the selective impairment of O-GlcNAcylated-tau and of O-GlcNAcylated-Complex I subunit NDUFB8, thus resulting in tau toxicity and reduced respiratory chain functionality respectively, highlighting the involvement of this posttranslational modification in the neurodegenerative process.

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Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice

Scientific Reports ◽

10.1038/s41598-020-80940-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Maki Murakoshi ◽

Tomohito Gohda ◽

Eri Adachi ◽

Saki Ichikawa ◽

Shinji Hagiwara ◽

...

Keyword(s):

Adipose Tissue ◽

High Fat Diet ◽

Kidney Injury ◽

Proximal Tubules ◽

Tubular Damage ◽

Standard Diet ◽

Mrna Levels ◽

High Fat ◽

Organ Specific

AbstractProgranulin (PGRN) has been reported to bind tumor necrosis factor (TNF) receptor and to inhibit TNFα signaling. We evaluated the effect of augmentation of TNFα signaling by PGRN deficiency on the progression of kidney injury. Eight-week-old PGRN knockout (KO) and wild-type (WT) mice were fed a standard diet or high-fat diet (HFD) for 12 weeks. Albuminuria, markers of tubular damage, and renal mRNA levels of inflammatory cytokines were higher in HFD-fed KO (KO-HFD) mice than in HFD-fed WT (WT-HFD) mice. Body weight, vacuolization in proximal tubules, and systemic and adipose tissue inflammatory markers were lower in the KO-HFD mice than in the WT-HFD mice. The renal megalin expression was lower in the KO mice than in the WT mice regardless of the diet type. The megalin expression was also reduced in mouse proximal tubule epithelial cells stimulated with TNFα and in those with PGRN knockdown by small interfering RNA in vitro. PGRN deficiency was associated with both exacerbated renal inflammation and decreased systemic inflammation, including that in the adipose tissue of mice with HFD-induced obesity. Improved tubular vacuolization in the KO-HFD mice might partially be explained by the decreased expression of megalin in proximal tubules.

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Perinatal High-Fat Diet Influences Ozone-Induced Responses on Pulmonary Oxidant Status and the Molecular Control of Mito-Phagy in Female Rat Offspring

International Journal of Molecular Sciences ◽

10.3390/ijms22147551 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7551

Author(s):

Sven H. Rouschop ◽

Samantha J. Snow ◽

Urmila P. Kodavanti ◽

Marie-José Drittij ◽

Lou M. Maas ◽

...

Keyword(s):

Oxidative Phosphorylation ◽

High Fat Diet ◽

Control Diet ◽

Ozone Exposure ◽

Female Rat ◽

Induced Responses ◽

High Fat ◽

Molecular Control ◽

Rat Offspring ◽

Oxidant Status

Previous research has shown that a perinatal obesogenic, high-fat diet (HFD) is able to exacerbate ozone-induced adverse effects on lung function, injury, and inflammation in offspring, and it has been suggested that mitochondrial dysfunction is implicated herein. The aim of this study was to investigate whether a perinatal obesogenic HFD affects ozone-induced changes in offspring pulmonary oxidant status and the molecular control of mitochondrial function. For this purpose, female Long-Evans rats were fed a control diet or HFD before and during gestation, and during lactation, after which the offspring were acutely exposed to filtered air or ozone at a young-adult age (forty days). Directly following this exposure, the offspring lungs were examined for markers related to oxidative stress; oxidative phosphorylation; and mitochondrial fusion, fission, biogenesis, and mitophagy. Acute ozone exposure significantly increased pulmonary oxidant status and upregulated the molecular machinery that controls receptor-mediated mitophagy. In female offspring, a perinatal HFD exacerbated these responses, whereas in male offspring, responses were similar for both diet groups. The expression of the genes and proteins involved in oxidative phosphorylation and mitochondrial biogenesis, fusion, and fission was not affected by ozone exposure or perinatal HFD. These findings suggest that a perinatal HFD influences ozone-induced responses on pulmonary oxidant status and the molecular control of mitophagy in female rat offspring.

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