scholarly journals Chemical investigation, toxic potential and acetylcholinesterase inhibitory effect of Parkia platycephala leaf and seed extracts

2021 ◽  
Vol 15 (9) ◽  
pp. 401-412
Author(s):  
de Moura Nunes Fernandes Rachel ◽  
Angelica Melo Rodrigues Maria ◽  
Farinelli Panontin Juliane ◽  
Ribeiro Alves Daniela ◽  
Maia Morais Selene ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Chemical Investigation ◽  
Toxic Potential ◽  
Seed Extracts

scholarly journals Anti-Aging Potential of Extracts from Washingtonia filifera Seeds

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 151
Author(s):  
Benedetta Era ◽  
Sonia Floris ◽  
Valeria Sogos ◽  
Clara Porcedda ◽  
Alessandra Piras ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Pharmaceutical Preparation ◽  
Human Keratinocytes ◽  
Inhibitory Effect ◽  
Skin Aging ◽  
Stressed Cells ◽  
Collagenase Inhibition ◽  
Washingtonia Filifera ◽  
Seed Extracts

The aim of this study was to test the inhibitory effect of fruit extracts from Washingtonia filifera on skin aging-related enzymes. The pulp extracts did not exert a significant enzyme inhibition while seed extracts from W. filifera exhibit anti-elastase, anti-collagenase, and anti-tyrosinase activities. Tyrosinase was mildly inhibited while a stronger effect was observed with respect to elastase and collagenase inhibition. Alcoholic extracts provided better results than aqueous extracts. Among them, methanol extracts showed the prominent enzyme inhibitory activities being IC50 value for elastase and collagenase comparable and even better than the reference compound. The inhibition mode of the most active extracts was investigated by Lineweaver-Burk plot analysis. Seed extracts from W. filifera were also investigated for their photo-protective effect by Mansur equation and the antioxidant activity of W. filifera extract was evaluated in oxidative-stressed cells. To evaluate the safety of the extract, the effect on cell viability of human keratinocytes cells was analyzed. Methanol extract presented the best photo-protective effect and exerted an antioxidant activity in a cellular system with no cytotoxic effect. The overall results demonstrate that W. filifera extracts are promising sources of bioactive compounds that could be used in cosmetic and pharmaceutical preparation.

scholarly journals Enhancing the enzymatic browning inhibition capacity of Moringa oleifera seed extract via the Maillard reaction

2021 ◽  
Vol 21 (103) ◽  
pp. 18518-18532
Author(s):  
Norah Vhangani Lusani ◽  
◽  
L Mogashoa ◽  
J Van Wyk
Keyword(s):  
Maillard Reaction ◽  
Inhibitory Effect ◽  
Model Systems ◽  
Enzymatic Browning ◽  
Brown Pigment ◽  
Pigment Formation ◽  
Browning Inhibition ◽  
Seed Extracts

The antioxidant and anti-browning activity of heated plant extracts have been attributed to the formation of Maillard reaction products (MRPs) via the Maillard reaction (MR). The inhibitory effect of heated Moringa oleifera (MO) seed extract on banana polyphenol oxidase (PPO) was investigated. The Plain MO seed extracts and those with added glucose and glycine (1.5 mM each) were heated at 100°C for 15, 30, 60 and 120 min. The pH and brown colour development decreased and increased significantly (P <0.05) with increased reaction time, respectively, with heated moringa glucose-glycine HMGGL for 120 min exhibiting the highest pH reduction (2.58) and darkest extracts at an L* value of 8.11. This phenomenon is associated with progression of the MR. With reference to enzymatic browning, heated MO seed extracts exhibited stronger inhibitory effect against banana PPO activity in vivo and in vitro than the unheated counterpart. Evident to this are the higher inhibition percentages and lower ΔE values. Among model systems, the highest in vitro browning inhibition was exhibited mostly by longer heating times of 60 and 120 min. Model system HMGGL 120 min proved to be superior at 96% inhibition, which was comparable to known synthetic commercial antioxidants such as ascorbic acid (AA) at 99%, as well as ethylenediaminetetraacetic acid (EDTA) and citric acid (CA), both at 100% inhibition. In vivo enzymatic browning inhibition followed a similar trend, where the brown pigment (melanin) intensified as shown by an increase in ΔE as the storage time increased from 0.5 to 24 h. The model system UMGGL exhibited highest inhibition of brown melanin (p <0.05). Although it was the best amongst other model systems, it was surpassed by synthetic antioxidants AA, EDTA and CA, which were ranked amongst the top three in inhibiting brown pigment formation in vivo. To further illustrate the effect of MR augmented MO seed extracts on enzyme activity inhibition, UMGGL 60 and 120 at 5 and 24 h storage surpassed the inhibitory effect of AA. At the said storage times, AA lost its inhibitory potential against pigment formation. This was due to oxidation of AA to form dehydroascorbic acid, which lacks inhibitory potential. This study proved that heating MO plant extracts increases their enzymatic browning inhibition potential, furthermore, the inhibitory capacity was heightened when reacted via the MR.

scholarly journals α-Glucosidase Inhibitory Activity of Phenolic Rich Extracts Obtained from the Seeds of Melastoma saigonense (Kuntze) Merr.

2019 ◽  
Vol 31 (12) ◽  
pp. 2964-2968 ◽  
Author(s):  
Nutthamon Prajudtasri ◽  
Mongkol Nontakitticharoen ◽  
Sujint Anguravirutt
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Antidiabetic Agent ◽  
Seed Extracts

The aim of this study was to perform a phytochemical analysis of Melastoma saigonense seed extracts and to determine their α-glucosidase inhibitory activity. The extracts from seeds of M. saigonense indicated that the total phenolic content was in the range between 233.46 and 967.22 mg GAE/g DE, whereas the flavonoids content was in the range between 359.96 and 850.84 mg QE/g DE. The present study of antidiabetic inhibitory activity by in vitro α-glucosidase revealed that the crude extracts using ethyl acetate (EA), butanol (BU) and final aqueous residue extracts (AQ) exhibited a strong α-glucosidase inhibitory effect (IC50 4.42-11.95 μg/mL). The ethyl acetate and butanol extracts of seeds of Melastoma saigonense (Kuntze) Merr. were further fractionated by silica gel column chromatography into four fractions (EAF1−EAF4) and five fractions (BUF1−BUF5), respectively and their bioactivities were investigated. The nine fractions exhibited significant α-glucosidase inhibitory activity (p < 0.05) with an IC50 between 3.42-34.77 μg/mL which is less than the IC50 for standard acarbose (IC50 = 507.26 μg/mL). Among all the fractions, BUF1 and EAF1 exhibited high inhibitory activity against α-glucosidase with BUF1 showing the highest inhibitory activity (IC50 = 3.42 μg/mL). The dominant phenolic acids were sinapic, gallic, ferrulic, syringic, gallic and caffeic acids and the prominent flavonoids were myricetin and quercetin. These findings suggest that the seeds of M. saigonense have potential as a source of antidiabetic agent (s).

Ricinodols A–G: new tetracyclic triterpenoids as 11β-HSD1 inhibitors from Ricinodendron heudelotii

RSC Advances ◽  
2015 ◽  
Vol 5 (34) ◽  
pp. 26777-26784 ◽  
Author(s):  
Jin-Hai Yu ◽  
Yu Shen ◽  
Yan Wu ◽  
Ying Leng ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Chemical Investigation ◽  
Significant Inhibitory Effect ◽  
Ricinodendron Heudelotii

Chemical investigation of Ricinodendron heudelotii has returned seven new tetracyclic triterpenoids with moderate to significant inhibitory effect against 11β-HSD1 enzyme.

scholarly journals Inhibitory Potentials of Hunteria umbellata Seed Extracts on Some Key Enzymes of Diabetes Mellitus with Profiling of its Phenolic Constituents

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Habeeb A. Bankole
Keyword(s):  
Diabetes Mellitus ◽  
Phenolic Compounds ◽  
Kinetic Studies ◽  
Inhibitory Effect ◽  
Sorbitol Dehydrogenase ◽  
Coumaric Acid ◽  
Phenolic Profile ◽  
Ic50 Value ◽  
Phenolic Constituents ◽  
Seed Extracts

Background: The seeds of Hunteria umbellatais a common folkloric plant used in the management of Diabetes mellitus in Nigeria. Though there are many reports on its antidiabetic potential, there is a scarcity of information on its mechanisms of antidiabetic action. This study was designed to investigate the mechanism of antidiabetic action of H. umbellata seed by evaluating the inhibitory effect of its extracts on enzymes that are targeted in the management of diabetes mellitus. Methods: Dried seeds of H. umbellata were powdered and extracted separately in water and ethanol. Alpha-amylase, α-glucosidase, and sorbitol dehydrogenase inhibitory activities of the concentrated extracts were determined spectrophotometrically. Furthermore, the phenolic profile of the seed was determined using HPLC-UV analysis. Results: The inhibitory effect of both ethanol and aqueous extracts H. umbellataon the selected enzymes were similar with IC50 value of 40.36 ± 2.92 and 40.25 ± 4.53 mg/mL for α-amylase, 45.42 ±2.44 and 39.32 ± 3.46 mg/mL for α-glucosidase, and 36.25 ± 2.31 and 32.35 ± 1.53 mg/mL for sorbitol dehydrogenase, respectively. Kinetic studies showed that α-amylase was inhibited competitively, while both α-glucosidase and sorbitol dehydrogenase were inhibited in a non-competitive manner. The phenolic compounds identified in the H. umbellata seed were gallic acid, catechin, p-coumaric acid, rutin, and ferulic acid. Conclusion: The H. umbellata seed extract’s antidiabetic action may be by inhibiting the activities of α-amylase, α-glucosidase, and sorbitol dehydrogenase. This inhibition could be due to the presence of phytochemicals such as phenolic compounds in the plant.

scholarly journals Biological properties of Oncocalyxone A: a review

2021 ◽  
Vol 10 (4) ◽  
pp. e57810414343
Author(s):  
Raí Emanuel da Silva ◽  
Fábio de Oliveira Silva Ribeiro ◽  
Gisele Santos de Araújo ◽  
Bruno Iles ◽  
Otília Deusdênia Loiola Pessoa ◽  
...  
Keyword(s):  
Biological Activity ◽  
Biological Activities ◽  
Normal Skin ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Antibiofilm Activity ◽  
Isolation And Characterization ◽  
Antitumor Potential ◽  
Toxic Potential

Quinones are a classic example of molecules isolated from natural products. These compounds are secondary metabolites produced by plants, fungi, bacteria, and insects and correspond to an interesting chemical class ubiquitously found in nature. The [rel-8α-Hydroxy-5-hydroxymethyl-2-methoxy-8α, β-methyl-7, 8, 8a, 9-tetahydro-1, 4-anthracenedione] [oncocalyxone A, onco-A] is a benzoquinone of dark red color isolated from the ethanolic extract of the species Cordia oncocalyx (Auxemma oncocalyx Allem). In this review, we aimed to investigate the main biological activities described for oncocalyxone A, as well as its isolation and characterization processes. For this review, the following descriptors were employed in multiple databases: medicinal plants, quinones, Auxemma oncocalyx, Cordia oncocalyx, biological activity. Besides, experimental papers published in international journals and with full text available were used as inclusion criteria. The biological activity profiles found for onco-A according to the literature were: antiproliferative/cytotoxic effects against SW1573 (alveolar cell carcinoma) and CCD922 (normal skin fibroblasts) cell lines; anti-inflammatory and analgesic potential; inhibitory effect on cell growth and DNA reactivity; antiplatelet activity; antitumor potential; anti-glycation agent; beneficial and/or toxic potential in goats and neuroinhibitory effect; antibacterial and antibiofilm activity. The substance has a broad spectrum of pharmacological applications, most of which are associated with its cytotoxic activity. The work also intends to compile the activities described in the literature, to understand how the molecule acts on the various targets, but, in some cases, more in-depth research is needed to elucidate the mechanisms of action.

Inhibitory Effect of Garcinia kola Heckel (Clusiacea) Seed Extracts on Some Key Enzymes Linked to Diabetes Mellitus

2015 ◽  
Vol 19 ◽  
pp. 139-144
Author(s):  
AM Zakariya ◽  
UH Danmalam ◽  
AB Sallau ◽  
G Ibrahim ◽  
SM Hassan
Keyword(s):  
Diabetes Mellitus ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Inhibitory Effect ◽  
Bioactive Constituents ◽  
Porcine Pancreas ◽  
Garcinia Kola ◽  
Soxhlet Apparatus ◽  
Seed Extracts

Enzymes linked with diabetes mellitus and Phytochemical characterization of its bioactive constituents. Crude n-hexane, ethyl acetate and methanol extracts were prepared successively in a soxhlet apparatus at 50ºC. Subsequently, extraction of crude enzymes from porcine pancreas and rat intestine, thin layer chromatographic analysis (TLC), and inhibitory effects of the extracts on b-amylase and b-glucosidase were determined in vitro. The results reveal that G. kola seed extracts inhibited b-amylase (IC50=  4.89 mg/ml, 3.44 mg/ml, 0.78 mg/ml) and b-glucosidase (IC50= 10.29 mg/ml, 1.68 mg/ml, 2.67 mg/ ml) corresponding to n-hexane, ethyl acetate, methanol gradient extracts. n-hexane extract had the highest inhibitory effect on b-amylase, while methanol extract was highest on b-glucosidase. Characterization of the bioactive constituents of the extracts using TLC analysis revealed the presence of steroids/triterpenes and phenolic compounds with phenolic compounds appearing in all extracts irrespective of the solvent polarity used for extraction. Anthraquinone and alkaloids were confirmed absent on TLC. This enzyme inhibition could be one of the possible pathways by which G. kola elicits its antidiabetic properties. Furthermore, the enzyme inhibitory properties of the extracts can be attributed to the presence of steroids/triterpenes, phenolic compounds in the seeds of G. kola.Keywords: Garcinia kola, b-amylase, b-glucosidase, TLC, Inhibitory Effect

scholarly journals Isolation of some active materials and aqueous, alcoholic and oil seed extracts of the plant (Curcuma longa (and study Antibacterial and anticancer activity

2013 ◽  
Vol 10 (3) ◽  
pp. 883-891
Author(s):  
Baghdad Science Journal
Keyword(s):  
Pathogenic Bacteria ◽  
Curcuma Longa ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Alcoholic Extract ◽  
Active Materials ◽  
Antibacterial And Anticancer Activity ◽  
Seed Extracts

This study included isolation of some active materials from Curcuma longa such as tannins, saponins and volatile oils with percentage of 59%, 31%, and 9% respectively. Also the study included the determination of minerals in Curcuma longa such as " Na, Ca and K" using Flame photometer. The concentrations of these minerals were (14 ppm),(10 ppm) and )76 ppm) respectively. The anti-bacterial activity study was performed for the active materials isolated from Curcuma longa against two genus of pathogenic bacteria, Escherichia Coli and Staphylococcus aurous by using agar-well diffusion method. It appeared from this study that all of the extraction have inhibitory effect on bacteria was used. The inhibition zone diameter varies with the type of active compound, its concentration and the types of bacteria. The results obtained were analyzed statistically way (one way ANOVA) she stated results that alcoholic extract warm has had the greatest influence on the ratio of the number of cells developing and the effect was significantly (P

scholarly journals Chemical Investigation of a Co-Culture of Aspergillus fumigatus D and Fusarium oxysporum R1

2020 ◽  
Vol 15 (2) ◽  
pp. 130-135
Author(s):  
Ronglu Yu ◽  
Mingzhu Li ◽  
Yi Wang ◽  
Xuelian Bai ◽  
Jianwei Chen ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Fusarium Oxysporum ◽  
Optical Rotation ◽  
2D Nmr ◽  
Inhibitory Effect ◽  
Chemical Investigation ◽  
Human Pathogens ◽  
Traditional Medicinal Plants ◽  
Staphyloccocus Aureus ◽  
Strong Inhibitory Effect

Chemical investigation of a co-culture of two endophytic fungi Aspergillus fumigatus D and Fusarium oxysporum R1 from two traditional medicinal plants, Edgeworthia chrysantha Lindl. and Rumex madaio Makino, led to isolation of a new amide 1 and six known compounds, including neovasinin (2), neovasifuranone B (3), N-(2-phenylethyl)acetamide (4), α-linolenic acid (5), α-elaeostearic acid (6), palmitoleic acid (7). On the basis of extensive spectroscopic analysis including 1D and 2D NMR, HR-ESI-MS and optical rotation measurement as well as comparison of literature data, chemical structure of 1 was unambiguous elucidated as (S, E)-methyl-2-(2,4-dimethylhex-2-enamido)acetate. Bioassay results indicated that none of these compounds exhibited strong inhibitory effect on three human pathogens Escherichia coli, Staphyloccocus aureus and Candida albicans with MIC values of ≥25 μM.

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