scholarly journals Antibacterial and antibiofilm activity of selected polyphenolic compounds: An in vitro study on Staphylococcus aureus

2020 ◽  
pp. 57-61
Author(s):  
Marina Kostić ◽  
Marija Ivanov ◽  
Dejan Stojković ◽  
Ana Ćirić ◽  
Marina Soković
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Study ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Antibiofilm Activity ◽  
Microdilution Method ◽  
Starting Point ◽  
Serious Infections ◽  
Common Infections

Staphylococcus aureus is a human pathogen that causes serious infections with high mortality rates. The antimicrobial activities of eight phenolic compounds (caffeic acid, chlorogenic acid, ferulic acid, morin, quercetin, isoquercitrin, rutin and hesperidin) were evaluated against planktonic and biofilm forming Staphylococcus aureus cells. Their ability to prevent biofilm formation via interference with bacterial cell adhesion and to reduce biomass of 24 h old biofilms has been addressed in this study. The antibacterial activities (MIC/MBC) were demonstrated using the microdilution method and the investigated compounds showed good activity against S. aureus isolates, with MICs in the range 0.05-0.4 mg/mL. Also, they exhibited promising antibiofilm potential in dose depended manner. Among all tested compounds, morin and quercetin showed the best antibiofilm activity at MIC values. The observed antimicrobial potential of the studied natural products can serve as a starting point towards development of novel plant-based therapeutics for the treatment of common infections such as the ones caused by S. aureus.

scholarly journals In Vitro Antibacterial and Antibiofilm Activity of Lippia alba Essential Oil, Citral, and Carvone against Staphylococcus aureus

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Emanuela Mesquita Porfírio ◽  
Hider Machado Melo ◽  
Antônio Matheus Gomes Pereira ◽  
Theodora Thays Arruda Cavalcante ◽  
Geovany Amorim Gomes ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Microtiter Plate ◽  
Antibiofilm Activity ◽  
Bacterial Cells ◽  
Lippia Alba ◽  
Microdilution Method ◽  
Aerial Parts ◽  
Clinical Interest

In vitro antimicrobial and antibiofilm activities of the Lippia alba essential oil and its major components (citral and carvone) against Staphylococcus aureus were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three L. alba specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL−1 when LA1EO and citral were used. Potential inhibition (100%) of S. aureus biofilm formation at the concentration of 0.5 mg mL−1 of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL−1, 2 mg mL−1, 2 mg mL−1, and 0.5 mg mL−1 for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of L. alba EOs against S. aureus, a species of recognized clinical interest.

scholarly journals Antimicrobial activities of widely consumed herbal tea’s alone or in combination with antibiotics: An in vitro study

2016 ◽  
Author(s):  
Mayram Tuysuz ◽  
Sibel Dosler ◽  
Ayse Seher Birteksoz Tan ◽  
Gulten Otuk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
In Vitro Study ◽  
Antimicrobial Activities ◽  
Combination Studies ◽  
Kill Curve ◽  
Time Kill ◽  
Microbroth Dilution ◽  
Antagonistic Interactions

Background: Because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives, which are gaining even more importance. We examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against the standard and clinical isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, methicillin susceptible/resistant Staphylococcus aureus and Candida albicans. Methods: The antimicrobial activities of the teas were determined by using the disk diffusion and microbroth dilution methods, and the combination studies were examined by using the microbroth checkerboard and time killing curve methods. Results: Rosehip, rosehip bag, pomegranate blossom, thyme, wormwood, mint, echinacea bag, cinnamon, black, and green teas were active against most of the studied microorganisms. In the combination studies, we characterized all the expected effects (synergistic, additive, and antagonistic) between the teas and the antimicrobials. While synergy was observed more frequently between ampicillin, ampicillin-sulbactam, or nystatine, and the various tea combinations, most of the effects between the ciprofloxacin, erythromycin, cefuroxime, or amikacin and various tea combinations, particularly rosehip, rosehip bag, and pomegranate blossom teas, were antagonistic. The results of the time kill curve analyses showed that none of the herbal teas were bactericidal in their usage concentrations; however, in combination they were. Discussion: Some herbal teas, particularly rosehip and pomegranate blossom should be avoided because of antagonistic interactions during the course of antibiotic treatment or should be consumed alone.

scholarly journals Comparative study of antimicrobial effect of brihati [solanum indicum linn ] root and fruit- an in vitro study.

2019 ◽  
Vol 7 (05) ◽  
Author(s):  
Sampada Dhongde ◽  
Shashidhar Naik
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Comparative Study ◽  
Wound Infection ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Literature Mining ◽  
Whole Plant

Brihati is a well known drug used in various diseases like dyspnoea, odema, wound infection etc1 .Both root and fruit are useful part of plant according to brihatrayi2-11.The study is undertaken for antimicrobial activity of root and fruit against Staphylococcus aureus and Staphylococcus Caprae. If  antimicrobial  effect of root and fruit is similar than it is our duty to save the plant. No  need to pluck the whole  plant or root and use only fruit extracts for antimicrobial activity.However the literature mining reveled that brihati have not been studied for antimicrobial activities, hence the present study is first systematic attempt to analyze the antimicrobial potential of Brihati on selected strains.

scholarly journals Antimicrobial activities of widely consumed herbal tea’s alone or in combination with antibiotics: An in vitro study

2016 ◽  
Author(s):  
Mayram Tuysuz ◽  
Sibel Dosler ◽  
Ayse Seher Birteksoz Tan ◽  
Gulten Otuk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
In Vitro Study ◽  
Antimicrobial Activities ◽  
Combination Studies ◽  
Kill Curve ◽  
Time Kill ◽  
Microbroth Dilution ◽  
Antagonistic Interactions

Background: Because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives, which are gaining even more importance. We examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against the standard and clinical isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, methicillin susceptible/resistant Staphylococcus aureus and Candida albicans. Methods: The antimicrobial activities of the teas were determined by using the disk diffusion and microbroth dilution methods, and the combination studies were examined by using the microbroth checkerboard and time killing curve methods. Results: Rosehip, rosehip bag, pomegranate blossom, thyme, wormwood, mint, echinacea bag, cinnamon, black, and green teas were active against most of the studied microorganisms. In the combination studies, we characterized all the expected effects (synergistic, additive, and antagonistic) between the teas and the antimicrobials. While synergy was observed more frequently between ampicillin, ampicillin-sulbactam, or nystatine, and the various tea combinations, most of the effects between the ciprofloxacin, erythromycin, cefuroxime, or amikacin and various tea combinations, particularly rosehip, rosehip bag, and pomegranate blossom teas, were antagonistic. The results of the time kill curve analyses showed that none of the herbal teas were bactericidal in their usage concentrations; however, in combination they were. Discussion: Some herbal teas, particularly rosehip and pomegranate blossom should be avoided because of antagonistic interactions during the course of antibiotic treatment or should be consumed alone.

scholarly journals An In Vitro Study: Antioxidant, Phytochemical Analysis and Antimicrobial Activities of Achranthes aspera

2021 ◽  
pp. 96-105
Author(s):  
Dipeshkumar Patel ◽  
Falaknaaz Shaikh
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Antibacterial And Antifungal Activities

Medicaments, plants and plant-based are the basis of many of the modern pharmaceuticals we use today for our various purposes. The aim of the present study was to evaluate the antioxidant, phytochemical and antibacterial and antifungal activities of the Achyranthes aspera plant extract in different organic solvents. The radical scavenging activity of the different extracts of root, stem, leaf, and seed were evaluated by DPPH assay and the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, and antifungal activity against Fusarium sp. and Aspergillus nigerwas studied by Agar well cut diffusion method. All of the extracts exhibited different antioxidant and antibacterial activities and the activities varied from solvent to solvent, and the activities are concentrated. The antioxidant and antimicrobial activities were compared with the positive control Ascorbic acid and Cefuroxime. A qualitative phytochemical analysis was carried out and found to possess bioactive compounds like alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins.

scholarly journals In Vitro Antibacterial Effect of the Methanolic Extract of the Korean Soybean Fermented Product Doenjang against Staphylococcus aureus

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2319
Author(s):  
Klara Lalouckova ◽  
Lucie Mala ◽  
Petr Marsik ◽  
Eva Skrivanova
Keyword(s):  
Staphylococcus Aureus ◽  
High Performance ◽  
Methanolic Extract ◽  
Bioactive Substances ◽  
Microdilution Method ◽  
Antibacterial Compound ◽  
Fermented Product ◽  
Broth Microdilution Method

Ultra-high performance liquid chromatography/mass spectrometry showed soyasaponin I and the isoflavones daidzein, genistein, and glycitein to be the main components of the methanolic extract of the Korean soybean fermented product doenjang, which is known to be a rich source of naturally occurring bioactive substances, at average contents of 515.40, 236.30, 131.23, and 29.00 ng/mg, respectively. The antimicrobial activity of the methanolic extract of doenjang against nine Staphylococcusaureus strains was determined in vitro by the broth microdilution method to investigate its potential to serve as an alternative antibacterial compound. The results suggest that the extract is an effective antistaphylococcal agent at concentrations of 2048–4096 µg/mL. Moreover, the tested extract also showed the ability to inhibit the growth of both methicillin-sensitive and methicillin-resistant animal and clinical S. aureus isolates. The growth kinetics of the chosen strains of S. aureus at the minimum inhibitory concentration of the methanolic extract of doenjang support the idea that the tested extract acts as an antibacterial compound. To the best of our knowledge, this is the first report on the antistaphylococcal action of the methanolic extract of doenjang thus, additional studies including in vivo testing are necessary to confirm this hypothesis.

scholarly journals In VitroActivities of Daptomycin-, Vancomycin-, and Teicoplanin-Loaded Polymethylmethacrylate against Methicillin-Susceptible, Methicillin-Resistant, and Vancomycin-Intermediate Strains of Staphylococcus aureus

2011 ◽  
Vol 55 (12) ◽  
pp. 5480-5484 ◽  
Author(s):  
Yuhan Chang ◽  
Wen-Chien Chen ◽  
Pang-Hsin Hsieh ◽  
Dave W. Chen ◽  
Mel S. Lee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
High Performance ◽  
Low Dose ◽  
Antibacterial Activities ◽  
High Dose ◽  
Bone Cements ◽  
Antibacterial Effects ◽  
Methicillin Resistant ◽  
Content Type

ABSTRACTThe objective of this study was to evaluate the antibacterial effects of polymethylmethacrylate (PMMA) bone cements loaded with daptomycin, vancomycin, and teicoplanin against methicillin-susceptibleStaphylococcus aureus(MSSA), methicillin-resistantStaphylococcus aureus(MRSA), and vancomycin-intermediateStaphylococcus aureus(VISA) strains. Standardized cement specimens made from 40 g PMMA loaded with 1 g (low-dose), 4 g (middle-dose) or 8 g (high-dose) antibiotics were tested for elution characteristics and antibacterial activities. The patterns of release of antibiotics from the cement specimens were evaluated usingin vitrobroth elution assay with high-performance liquid chromatography. The activities of broth elution fluid against differentStaphylococcus aureusstrains (MSSA, MRSA, and VISA) were then determined. The antibacterial activities of all the tested antibiotics were maintained after being mixed with PMMA. The cements loaded with higher dosages of antibiotics showed longer elution periods. Regardless of the antibiotic loading dose, the teicoplanin-loaded cements showed better elution efficacy and provided longer inhibitory periods against MSSA, MRSA, and VISA than cements loaded with the same dose of vancomycin or daptomycin. Regarding the choice of antibiotics for cement loading in the treatment ofStaphylococcus aureusinfection, teicoplanin was superior in terms of antibacterial effects.

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