Detection of biofilm formation ability of Streptococcus agalactiae isolated from bovine mastitis cases

2019 ◽  
Vol 9 (1) ◽  
pp. 107
Author(s):  
M Nasim Sohail ◽  
D Rathnamma ◽  
S Isloor ◽  
BM Veeregowda ◽  
R Sharada
Keyword(s):  
Streptococcus Agalactiae ◽  
Biofilm Formation ◽  
Bovine Mastitis

scholarly journals Anti-Biofilm Effect of Tea Saponin on a Streptococcus agalactiae Strain Isolated from Bovine Mastitis

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1713
Author(s):  
Fei Shang ◽  
Hui Wang ◽  
Ting Xue
Keyword(s):  
Streptococcus Agalactiae ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Antibacterial Activities ◽  
Economic Losses ◽  
Neonatal Meningitis ◽  
Tea Saponin ◽  
Polymerase Chain ◽  
Biofilm Structure

Streptococcus agalactiae (GBS) is a highly contagious pathogen which not only can cause neonatal meningitis, pneumonia, and septicemia but is also considered to be a major cause of bovine mastitis (BM), leading to large economic losses to the dairy industry worldwide. Like many other pathogenic bacteria, GBS also has the capacity to form a biofilm structure in the host to cause persistent infection. Tea saponin (TS), is one of the main active agents extracted from tea ash powder, and it has good antioxidant and antibacterial activities. In this study, we confirmed that TS has a slight antibacterial activity against a Streptococcus agalactiae strain isolated from dairy cow with mastitis and inhibits its biofilm formation. By performing scanning electron microscopy (SEM) experiments, we observed that with addition of TS, the biofilm formed by this GBS strain exhibited looser structure and lower density. In addition, the results of real-time reverse transcription polymerase chain reaction (RT-PCR) experiments showed that TS inhibited biofilm formation by down-regulating the transcription of the biofilm-associated genes including srtA, fbsC, neuA, and cpsE.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

scholarly journals Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)

2014 ◽  
Vol 17 (2) ◽  
pp. 321-329 ◽  
Author(s):  
K. Wolska ◽  
P. Szweda ◽  
K. Lada ◽  
E. Rytel ◽  
K. Gucwa ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bovine Mastitis ◽  
Twitching Motility ◽  
Phenotypic Properties ◽  
Biofilm Production ◽  
Abiotic Surfaces ◽  
Hospital Patients ◽  
Relationship Of ◽  
Initial Biofilm ◽  
Different Sources

AbstractThe molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomic relationship of 28 P. aeruginosa strains isolated from faeces of healthy bovine, bovine mastitis and from faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealed large molecular differentiation within this group of isolates. Twenty two out of 28 strains tested generated unique patterns of DNA bands and only three genotypes consisted of two isolates each were identified. We also tested the P. aeruginosa isolates for their ability to form a biofilm on abiotic surfaces including polyvinylchloride and polystyrene. Different biofilm-forming abilities were demonstrated among strains; however, most of them (64.3%) showed moderate-biofilm forming ability. The strains with increased swimming and twitching motility displayed elevated biofilm formation. However, a negative correlation was found between slime and initial biofilm production. On the basis of the results obtained, we suggest that there are no major differences in phenotypic properties between P. aeruginosa strains isolated from different sources

scholarly journals A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2771
Author(s):  
Tobias Wieland ◽  
Julia Assmann ◽  
Astrid Bethe ◽  
Christian Fidelak ◽  
Helena Gmoser ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Real Time ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Inhibitory Effect ◽  
Thermal Sensor ◽  
Bacterial Cells ◽  
Static Conditions

The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated Escherichia (E.) coli and Staphylococcus (S.) aureus. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs.

scholarly journals Draft Genome Sequence of Streptococcus agalactiae Serotype Ia Strain M19, a Multidrug-Resistant Isolate from a Cow with Bovine Mastitis

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Feng Yang ◽  
Hongsheng Li ◽  
Shidong Zhang ◽  
Xurong Wang
Keyword(s):  
Genome Sequence ◽  
Streptococcus Agalactiae ◽  
Draft Genome ◽  
Bovine Mastitis ◽  
Multidrug Resistant ◽  
Draft Genome Sequence ◽  
Resistant Isolate ◽  
Draft Sequence ◽  
Autonomous Region ◽  
Ningxia Hui Autonomous Region

Streptococcus agalactiae is a major contagious pathogen causing bovine mastitis worldwide. We report here the draft sequence of S. agalactiae Ia strain M19, a multidrug-resistant isolate from a bovine mastitis case in Ningxia Hui autonomous region, China.

scholarly journals Evaluation of Anti-biofilm Formation of Methicillin Resistant Staphylococcus Species in Clinical Milk Samples of Bovine Mastitis using Selective Medicinal Plants

2022 ◽  
pp. 632-641
Author(s):  
Jayaprabha Chockalingam ◽  
Akshaya Balasubramanian ◽  
Jai Santhosh K. Krishnasamy ◽  
Karthika Balasundaram ◽  
Kavin Kumar Sivasamy ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Biofilm Formation ◽  
Bovine Mastitis ◽  
Methicillin Resistant ◽  
Milk Samples

scholarly journals Expression of icaA and icaD genes in biofilm formation in Staphylococcus aureus isolates from bovine subclinical mastitis

2021 ◽  
Vol 41 ◽  
Author(s):  
Viviane F. Marques ◽  
Huarrisson A. Santos ◽  
Thomas H. Santos ◽  
Dayanne A. Melo ◽  
Shana M.O. Coelho ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Phenotypic Expression ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Transcriptional Analysis ◽  
Important Species ◽  
High Prevalence ◽  
Staphylococcus Spp

ABSTRACT: Staphylococcus spp. plays a significant role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most important species due to the high prevalence and the difficulty of in vivo treatment that is related to the expression of virulence factors and biofilm formation. This study aimed to detect the phenotypic expression of the biofilm formation in 20 S. aureus isolated from bovine mastitis and to evaluate the expression and regulation of genes involved in its production. MALDI-TOF and phenogenotypic identification assays were performed to characterize the isolates. The phenotypic biofilm production and the presence of icaA and icaD and bap genes were evaluated. The Agr system was typified (agr I, agr II, agr III and agr IV) and its regulator (agr RNAIII) was detected. Furtherly, Real-time PCR (qPCR) was performed at chosen times to quantify the expression of icaA, icaD and hld genes in three selected isolates. All 20 strains were biofilm producers and most presented icaA and icaD genes. Only one isolate presented the bap gene. The agr gene type II showed a prevalence of 70%. Transcriptional analysis revealed increased expression of ica genes at eight hours of growth. These results confirm that polysaccharides production mediated by the icaADBC operon genes is an essential mechanism to the biofilm formation and contributes to the early stages of bacterial growth.

scholarly journals Phenotypic and Genotypic Characteristics and Resistance Profile of Staphylococcus spp. from Bovine Mastitis

2020 ◽  
Vol 48 ◽  
Author(s):  
Érica Chaves Lucio ◽  
Gisele Veneroni Gouveia ◽  
Mateus Matiuzzi Da Costa ◽  
Mário Baltazar De Oliveira ◽  
Rinaldo Aparecido Mota ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Milk Production ◽  
Biofilm Formation ◽  
Negative Impact ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Resistant Bacteria ◽  
Production Chain ◽  
Biofilm Production ◽  
Staphylococcus Spp

Background: Bovine mastitis remains one of the health problems that cause the most damage to milk producers. The negative impact of mastitis is due to reduced milk production, early slaughter of females, reduced commercial value of the affected animals, losses in the genetic potential of the herd, expenses with medicines and veterinary medical assistance. Staphylococcus spp. stands out as the cause of this disease and has been able to remain in the mammary gland, becoming resistant to several antimicrobials. The aims of the present study were to characterize the phenotypes, genotypes and resistance profiles of Staphylococcus spp. isolates from bovine mastitis cases in the state of Pernambuco, Brazil.Materials, Methods & Results: These isolates were classified according to biochemical tests and the presence of the nuc gene.  The polymerase chain reaction (PCR) for amplification of the mecA and blaZ genes was used to analyze the genetic potentials of antimicrobial resistance. Isolates were also phenotypically tested for resistance to nine antimicrobials (ampicillin, doxicillin, erythromycin, gentamicin, rifampicin, cephalothin, amoxicillin, nalidixic acid and oxacillin). The genetic potentials for biofilm production were evaluated by the amplifications of the icaD, icaA and bap genes. The phenotypic test of gentian violet was used for biofilm formation analyzes. Ninety-three (93.0%) of the isolates among the Staphylococcus spp. samples were classified as Staphylococcus aureus. The lowest percentage of sensitivity observed was for amoxicillin (28.0%). All of the isolates were sensitive to erythromycin and gentamicin, and 15 (15%) exhibited sensitivity to all of the drugs tested. All of the isolates were negative for the mecA gene, and 36 (36%) were positive for blaZ. In the adhesion microplate tests, 44 (44%) of the isolates were capable of biofilm formation. Of these, seven (15.9%) were strong formers, whereas 16 (36.3%) and 21 (47.8%) were moderate and weak formers, respectively. The icaD gene was confirmed in 89 (89%) of the isolates. The icaA gene was confirmed in 61 (61%) samples, and the bap gene in 52 (52%) samples. One of the samples did not possess icaA, icaD or bap and exhibited moderate biofilm formation according to the microplate adherence test. Sixteen isolates simultaneously exhibited the three genes tested for biofilm production (icaA, icaD and bap) and were negative according to the microplate adherence test.Discussion:  The indiscriminate use of antibiotics to treat mastitis is a common practice in the study area, which may have contributed to the high proportion of herds (88.23%; 15/17) with multi-resistant isolates, constituting a selection factor for the dissemination of resistant bacteria among herds.  The absence of the mecA gene in the present study may be associated with the development of resistant bacteria through another mechanism, such as the overproduction of beta-lactamases. The results demonstrate that antimicrobial resistance occurs in Staphylococcus spp. that cause bovine mastitis in herds of Pernambuco and that these isolates have the a great capacity for biofilm formation. It is necessary to sensitize the professionals involved in the milk production chain of Brazil regarding the importance of the adequate use of antimicrobials for the treatment and control of mastitis, since studies in the country indicate the dissemination of resistant bacterial strains.

scholarly journals Propolis in the control of bacterial bovine mastitis: a tool for the production of organic milk

2022 ◽  
Vol 43 (2) ◽  
pp. 869-882
Author(s):  
Gabriel Michelutti do Nascimento ◽  
◽  
Marita Vedovelli Cardozo ◽  
Mylena Karoline Valmorbida ◽  
Natália Pereira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Agalactiae ◽  
Bovine Mastitis ◽  
Dairy Farms ◽  
Economic Damage ◽  
Glycerol Solution ◽  
Bacterial Reduction ◽  
Organic Milk ◽  
Organic Dairy ◽  
Organic Dairy Farms

Bovine mastitis is one of the main causes of economic damage in dairy farms. Therefore, the control and prevention of microorganisms involved in this disease, mainly Escherichia coli, Staphylococcus aureus, and Streptococcus agalactiae, are essential. One of the most important steps for the prevention of the disease is the use of antiseptic products before and after the milking process to avoid bacteria from infecting the udder of the animal. Currently, the most used antiseptic product in dairy farms is iodine-based, and organic dairy farms, which follow several strict regulations, including the use of natural products whenever possible, are often forced to adopt non-natural antiseptic products, such as iodine-based ones, because of the lack of natural alternatives. Propolis, a natural substance produced by honeybees, has been extensively studied for its various properties, one of which is antimicrobial activity. Therefore, a new natural antiseptic product containing 1% propolis in 10% hydroalcoholic solution for the pre-dipping, and 10% glycerol solution added with 0.2% citronella oil for the post-dipping was analyzed for its capacity to reduce bacteria in vivo in order to prevent bovine mastitis, allowing its use on organic dairy farms. A total of 128 samples were analyzed in terms of bacterial growth for Enterobacteriaceae and Staphylococcus spp. using the spreadplate technique. The reduction in the bacterial concentration after the application of the products was compared between two antiseptic solutions, an iodine-based solution as the control and a propolis-based one as the natural alternative. The results obtained show a similar efficiency for both products in terms of total bacterial reduction, indicating considerable antimicrobial activity against bacteria most commonly associated with bovine mastitis. Molecular analysis was carried out for the identification of Streptococcus agalactiae; the PCR results were negative for the presence of S. agalactiae in all samples, indicating that the animals most likely did not have any form of the disease. The efficiency of the natural antiseptic was satisfactory, indicating an important find facilitating organic milk production worldwide, showcasing a natural antiseptic solution with efficient antimicrobial activity.

scholarly journals Bacteriophage-Derived PeptidaseCHAPKEliminates and Prevents Staphylococcal Biofilms

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Mark Fenton ◽  
Ruth Keary ◽  
Olivia McAuliffe ◽  
R. Paul Ross ◽  
Jim O'Mahony ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Antibacterial Agents ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Economic Losses ◽  
Food Sector ◽  
Staphylococcal Infections ◽  
Prevention And Treatment

New antibacterial agents are urgently needed for the elimination of biofilm-forming bacteria that are highly resistant to traditional antimicrobial agents. Proliferation of such bacteria can lead to significant economic losses in the agri-food sector. This study demonstrates the potential of the bacteriophage-derived peptidase,CHAPK, as a biocidal agent for the rapid disruption of biofilm-forming staphylococci, commonly associated with bovine mastitis. PurifiedCHAPKapplied to biofilms ofStaphylococcus aureusDPC5246 completely eliminated the staphylococcal biofilms within 4 h. In addition,CHAPKwas able to prevent biofilm formation by this strain. TheCHAPKlysin also reducedS. aureusin a skin decolonization model. Our data demonstrates the potential ofCHAPKas a biocidal agent for prevention and treatment of biofilm-associated staphylococcal infections or as a decontaminating agent in the food and healthcare sectors.

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