Cuticular hydrocarbon variation in rotenone induced and transgenically created parkinson’s disease (PD) flies of Drosophila melanogaster

2020 ◽  
Vol 39a (2) ◽  
pp. 281-287
Author(s):  
Ali Hatami Dehnow ◽  
Mysore Siddaiah Krishna
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Cuticular Hydrocarbon

scholarly journals Antioxidant Therapy in Parkinson’s Disease: Insights from Drosophila melanogaster

Antioxidants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 52 ◽  
Author(s):  
Federica De Lazzari ◽  
Federica Sandrelli ◽  
Alexander J. Whitworth ◽  
Marco Bisaglia
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Therapeutic Potential ◽  
Experimental System ◽  
Rapid Screening ◽  
Antioxidant Compounds ◽  
Oxygen Species ◽  
Protective Effects ◽  
High Concentrations

Reactive oxygen species (ROS) play an important role as endogenous mediators in several cellular signalling pathways. However, at high concentrations they can also exert deleterious effects by reacting with many macromolecules including DNA, proteins and lipids. The precise balance between ROS production and their removal via numerous enzymatic and nonenzymatic molecules is of fundamental importance for cell survival. Accordingly, many neurodegenerative disorders, including Parkinson’s disease (PD), are associated with excessive levels of ROS, which induce oxidative damage. With the aim of coping with the progression of PD, antioxidant compounds are currently receiving increasing attention as potential co-adjuvant molecules in the treatment of these diseases, and many studies have been performed to evaluate the purported protective effects of several antioxidant molecules. In the present review, we present and discuss the relevance of the use of Drosophila melanogaster as an animal model with which to evaluate the therapeutic potential of natural and synthetic antioxidants. The conservation of most of the PD-related genes between humans and D. melanogaster, along with the animal’s rapid life cycle and the versatility of genetic tools, makes fruit flies an ideal experimental system for rapid screening of antioxidant-based treatments.

The FBXO7 homologue nutcracker and binding partner PI31 in Drosophila melanogaster models of Parkinson’s disease

Genome ◽  
2017 ◽  
Vol 60 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Eric M. Merzetti ◽  
Lindsay A. Dolomount ◽  
Brian E. Staveley
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Severe Form ◽  
Altered Expression ◽  
Binding Partner ◽  
Locomotor Control ◽  
Dependent Model

Parkinsonian-pyramidal syndrome (PPS) is an early onset form of Parkinson’s disease (PD) that shows degeneration of the extrapyramidal region of the brain to result in a severe form of PD. The toxic protein build-up has been implicated in the onset of PPS. Protein removal is mediated by an intracellular proteasome complex: an E3 ubiquitin ligase, the targeting component, is essential for function. FBXO7 encodes the F-box component of the SCF E3 ubiquitin ligase linked to familial forms of PPS. The Drosophila melanogaster homologue nutcracker (ntc) and a binding partner, PI31, have been shown to be active in proteasome function. We show that altered expression of either ntc or PI31 in dopaminergic neurons leads to a decrease in longevity and locomotor ability, phenotypes both associated with models of PD. Furthermore, expression of ntc-RNAi in an established α-synuclein-dependent model of PD rescues the phenotypes of diminished longevity and locomotor control.

scholarly journals Signaling Pathways Involved in 1-Octen-3-ol-Mediated Neurotoxicity in Drosophila melanogaster: Implication in Parkinson’s Disease

2013 ◽  
Vol 25 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Arati A. Inamdar ◽  
Prakash Masurekar ◽  
Muhammad Hossain ◽  
Jason R. Richardson ◽  
Joan W. Bennett
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Signaling Pathways

scholarly journals An altered microbiome in a Parkinson’s disease model Drosophila melanogaster has a negative effect on development

2021 ◽  
Author(s):  
Jade Parker-Character ◽  
David R. Hager ◽  
Tanner B. Call ◽  
Zachary S. Pickup ◽  
Scott A. Turnbull ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Disease Model ◽  
Model Organism ◽  
Fecal Microbiota ◽  
Microbiota Composition ◽  
Host Genotype ◽  
Negative Effect ◽  
And Control

Abstract It is well-established that there are differences in the fecal microbiota composition between Parkinson’s disease (PD) patients and control populations, but the mechanisms underlying these differences are not yet fully understood. To begin to close the gap between description and mechanism we studied the relationship between the microbiota and PD in a model organism, Drosophila melanogaster. First, fecal transfers were performed with a D. melanogaster model of PD that had a mutation in the parkin (park25) gene. Results indicate that the PD model feces had a negative effect on both pupation and eclosion in both control and park25 flies, with a greater effect in PD model flies. Analysis of the microbiota composition revealed differences between the control and park25 flies, consistent with many human studies. Conversely, gnotobiotic treatment of axenic embryos with feces-derived bacterial cultures did not affect eclosure. We speculate this result might be due to similarities in bacterial prevalence between mutant and control feces. Further, we confirmed a bacteria-potentiated impact on mutant and control fly phenotypes by measuring eclosure rate in park25flies that were mono-associated with members of the fly microbiota. Both the fecal transfer and the mono-association results indicate a host genotype-microbiota interaction. Overall, this study concludes functional effects of the fly microbiota on PD model flies, providing support to the developing body of knowledge regarding the influence of the microbiota on PD.

scholarly journals An altered microbiome in a Parkinson’s disease model Drosophila melanogaster has a negative effect on development

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jade Parker-Character ◽  
David R. Hager ◽  
Tanner B. Call ◽  
Zachary S. Pickup ◽  
Scott A. Turnbull ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Disease Model ◽  
Model Organism ◽  
Fecal Microbiota ◽  
Microbiota Composition ◽  
Host Genotype ◽  
Negative Effect ◽  
And Control

AbstractParkinson’s disease (PD) is the second most common neurodegenerative disease, besides Alzheimer’s Disease, characterized by multiple symptoms, including the well-known motor dysfunctions. It is well-established that there are differences in the fecal microbiota composition between Parkinson’s disease (PD) patients and control populations, but the mechanisms underlying these differences are not yet fully understood. To begin to close the gap between description and mechanism we studied the relationship between the microbiota and PD in a model organism, Drosophila melanogaster. First, fecal transfers were performed with a D. melanogaster model of PD that had a mutation in the parkin (park25) gene. Results indicate that the PD model feces had a negative effect on both pupation and eclosion in both control and park25 flies, with a greater effect in PD model flies. Analysis of the microbiota composition revealed differences between the control and park25 flies, consistent with many human studies. Conversely, gnotobiotic treatment of axenic embryos with feces-derived bacterial cultures did not affect eclosure. We speculate this result might be due to similarities in bacterial prevalence between mutant and control feces. Further, we confirmed a bacteria-potentiated impact on mutant and control fly phenotypes by measuring eclosure rate in park25 flies that were mono-associated with members of the fly microbiota. Both the fecal transfer and the mono-association results indicate a host genotype-microbiota interaction. Overall, this study concludes functional effects of the fly microbiota on PD model flies, providing support to the developing body of knowledge regarding the influence of the microbiota on PD.

scholarly journals Genome Sequence of Acetobacter tropicalis DmPark25_167, a Bacterium Isolated from a Drosophila melanogaster Genetic Model of Parkinson’s Disease

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Julie A. Goddard ◽  
Samara C. Petersen ◽  
Gerald B. Call ◽  
John M. Chaston
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Genome Sequence ◽  
Bacterial Strain ◽  
Genetic Model ◽  
Type Iv Secretion ◽  
Methionine Metabolism ◽  
Content Type ◽  
Type Iv

ABSTRACT Here, we report the genome of Acetobacter tropicalis DmPark25_167, a bacterial strain isolated from a Drosophila melanogaster park25 mutant. The park25 mutant is an established genetic model of Parkinson’s disease. DmPark25_167 has duplicated methionine metabolism and type IV secretion gene alleles compared with another strain of A. tropicalis.

The neuroprotective effect of fullerenols on a model of Parkinson’s disease in Drosophila melanogaster

2020 ◽  
Vol 523 (2) ◽  
pp. 446-451
Author(s):  
I. Golomidov ◽  
O. Bolshakova ◽  
A. Komissarov ◽  
V. Sharoyko ◽  
Е. Slepneva ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Neuroprotective Effect

Drosophila melanogaster a versatile model of Parkinson’s Disease

2021 ◽  
Vol 20 ◽  
Author(s):  
Falaq Naz ◽  
Yasir Hasan Siddique
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Drosophila Melanogaster ◽  
Lewy Bodies ◽  
Substantia Nigra Pars Compacta ◽  
Maintenance Cost ◽  
The Novel ◽  
Life Threatening ◽  
Non Motor Symptoms ◽  
The Brain

: Parkinson's Disease (PD) is one of the most prevalent, recurrent and life-threatening neurodegenerative disease. However, the precise mechanism underlying this disease is not yet clearly understood. For understanding the pathogenesis of PD, it is essential to identify the symptoms along with the novel biological markers and to develop strategies which could lead towards the development of effective therapy. PD is associated with Lewy bodies (LBs) formation and the loss of dopaminergic neurons in the substantia nigra pars compacta of mid brain region. For the improvement in treatment strategiesas well as understanding the pathophysiology of the PD in number ofanimal models have been introduced that can recapitulatethe pathophysiology, motor and non-motor symptoms of PD. In contrast to mammalian models like rodents, mice and monkey, Drosophila is easy to handle as well as it maintenance cost is low.Due to the anatomical differencesin the brain and other major organsof human and fly,the issues of standardizing the methods or experiments to analyze behavioral aspects (walking, writhing, eating and sleeping) are difficult in flies. Thepresent review highlights the studies carried out for PD since 2000, using Drosophila melanogaster.

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