Effect of progesterone hormon on cell viability and stem cell activation in dental pulp cells

Abstract Background The purpose of this study was to assess the cytotoxicity of various concentrations of ozonated water (OW) on human primary dental pulp cells. Methods Human primary dental pulp cells were isolated from exfoliated primary canine teeth of an 11-year-old patient with good systemic and oral health. Afterwards, cells were divided into 6 experimental groups; four groups of OW in concentrations of 2 mg/L, 4 mg/L, 8 mg/L, and 16 mg/L, untreated control group, and cell culture without cells. Cytotoxicity was evaluated after exposure for 5-min exposure using Mosmann’s Tetrazolium Toxicity (MTT) assay at 0 h and 48 h time points. Data were analyzed using a repeated measures analysis of variance and Post-hoc tests were performed using Bonferroni correction for multiple comparisons. Results All experimental groups showed proliferation at 0 h time point. However, all groups also experienced a decrease in overtime at 48 h time point (p < 0.05). At both time points 2 mg/L OW showed the highest cell viability as well as proliferation. At 0 h time point, the increase in cell viability for all experimental groups was found statistically significant when compared to positive control group (p < 0.05). At 48 h time point, although 8 mg/L and 16 mg/L OW showed statistically significant reduction in compare to 0 h time point, 2 mg/L and 4 mg/L OW groups didn’t experience any statistically significant difference (p < 0.05). Conclusion Considering our findings, due to ozonated water's induced a higher proliferation rate of dental pulp cells, indicating their biocompatibility and a possible adjuvant on irrigating agent in regenerative endodontic procedures.

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Dental Pulp Cells for Induced Pluripotent Stem Cell Banking

Journal of Dental Research ◽

10.1177/0022034510366846 ◽

2010 ◽

Vol 89 (8) ◽

pp. 773-778 ◽

Cited By ~ 141

Author(s):

N. Tamaoki ◽

K. Takahashi ◽

T. Tanaka ◽

T. Ichisaka ◽

H. Aoki ◽

...

Keyword(s):

Stem Cell ◽

Dental Pulp ◽

Pluripotent Stem Cell ◽

Induced Pluripotent Stem Cell ◽

Dental Pulp Cells ◽

Cell Banking ◽

Pulp Cells ◽

Induced Pluripotent ◽

Stem Cell Banking

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miRNA-720 Controls Stem Cell Phenotype, Proliferation and Differentiation of Human Dental Pulp Cells

PLoS ONE ◽

10.1371/journal.pone.0083545 ◽

2013 ◽

Vol 8 (12) ◽

pp. e83545 ◽

Cited By ~ 35

Author(s):

Emilio Satoshi Hara ◽

Mitsuaki Ono ◽

Takanori Eguchi ◽

Satoshi Kubota ◽

Hai Thanh Pham ◽

...

Keyword(s):

Stem Cell ◽

Dental Pulp ◽

Cell Phenotype ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Proliferation And Differentiation ◽

Human Dental Pulp ◽

Pulp Cells ◽

Stem Cell Phenotype

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Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free Media

Journal of Endodontics ◽

10.1016/j.joen.2010.03.002 ◽

2010 ◽

Vol 36 (7) ◽

pp. 1139-1144 ◽

Cited By ~ 29

Author(s):

Thais Miyuki Hirata ◽

Nikolay Ishkitiev ◽

Ken Yaegaki ◽

Bogdan Calenic ◽

Hiroshi Ishikawa ◽

...

Keyword(s):

Stem Cell ◽

Dental Pulp ◽

Stem Cell Markers ◽

Dental Pulp Cells ◽

Serum Free ◽

Cell Markers ◽

Pulp Cells ◽

Free Media ◽

Cells Cultured

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TOXICITY ANALYSIS OF RGD-CHITOSAN FROM SHRIMP SHELL SCAFFOLD MEMBRANES TOWARD HUMAN DENTAL PULP CELLS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s1.07_08 ◽

2017 ◽

Vol 9 ◽

pp. 13

Author(s):

Mauldina Shabrina ◽

Dewi Fatma Suniarti ◽

Lisa R Amir ◽

Erik Idrus

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Control Group ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Membrane Toxicity ◽

Toxicity Analysis ◽

Human Dental Pulp ◽

Pulp Cells ◽

Group 2

Objective: This study aimed to analyze RGD-Chitosan from Shrimp Shells’ Scaffolds’ (RCSSS) and CSSS membrane toxicity toward human dental pulpcells.Methods: Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determinedusing an MTT assay method.Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSSgroup 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%).Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells comparedto CSSS membranes.

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Solvent and HEMA Increase Adhesive Toxicity and Cytokine Release from Dental Pulp Cells

Materials ◽

10.3390/ma12172750 ◽

2019 ◽

Vol 12 (17) ◽

pp. 2750 ◽

Cited By ~ 4

Author(s):

Helder Massaro ◽

Lígia Zambelli ◽

Auriléia Britto ◽

Rodolfo Vieira ◽

Ana Ligeiro-de-Oliveira ◽

...

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Culture Medium ◽

Control Group ◽

Dental Pulp Cells ◽

Cytokine Release ◽

Human Dental Pulp Cells ◽

Medium Components ◽

Tnf Α ◽

Pulp Cells

The aim of the present study was to evaluate the effect of the hydroxyethyl-methacrylate (HEMA) concentration and solvent content of dental adhesives on cell viability and cytokine (IL-1b, IL-6, IL-10, TNF-α) release by human dental pulp cells (HDPCs). HDPCs were obtained from fresh extracted human third molars. Experimental adhesives were prepared containing different concentrations of HEMA (0%, 10%, and 20%) with and without solvent (ethanol 10%). Cylindrical specimens were immersed on culture medium during 24 h to obtain the extracts. The cells were incubated with extracts (culture medium + components leached from the adhesives) of different adhesives, and cell viability and cytokine release were evaluated after 6 and 24 h of exposure. Adhesives containing HEMA promoted high cell viability reduction after 6 h of exposure; but after 24 h, the results were similar to the ones found among control group cells. These effects on cell viability were prominently increased with the addition of solvent. Although IL-1b release was not affected by exposure to eluates, other cytokines (IL-10, IL-6, TNF-α) were modulated by the different experiment conditions, directly influenced by the HEMA concentration and presence of solvent. Higher HEMA concentrations, combined with the presence of solvent, can promote significant reduction on HDPC viability, increasing the release of anti- and pro-inflammatory mediators.

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Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Archives of Histology and Cytology ◽

10.1679/aohc.72.51 ◽

2009 ◽

Vol 72 (1) ◽

pp. 51-64 ◽

Cited By ~ 45

Author(s):

Akiyuki Morito ◽

Yujiro Kida ◽

Kazunori Suzuki ◽

Koji Inoue ◽

Noriyuki Kuroda ◽

...

Keyword(s):

Stem Cell ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Dental Pulp ◽

Fibroblast Growth ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

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Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2Induced Toxicity on Dental Pulp Cells

BioMed Research International ◽

10.1155/2014/895049 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Fernanda da Silveira Vargas ◽

Diana Gabriela Soares ◽

Ana Paula Dias Ribeiro ◽

Josimeri Hebling ◽

Carlos Alberto De Souza Costa

Keyword(s):

Vitamin E ◽

Protective Effect ◽

Cell Viability ◽

Dental Pulp ◽

Positive Control ◽

Negative Control ◽

Alpha Tocopherol ◽

Dental Pulp Cells ◽

Cell Protection ◽

Pulp Cells

The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mMα-T for 60 minutes. They were then exposed or not to H2O2for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H2O2(0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests;α=5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated withα-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect ofα-T against the H2O2toxicity. Overall, the vitamin Eα-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2. The most effective cell protection was provided by 5 and 10 mM concentrations ofα-T.

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Cytotoxic Effect of Chitosan Nanoparticles on Normal Human Dental Pulp Cells

Nanoscience and Nanotechnology ◽

10.18063/nn.v3i1.940 ◽

2019 ◽

Vol 3 (1) ◽

Author(s):

Rami Alhomrany ◽

Chang Zhang ◽

Laisheng Chou

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Cell Attachment ◽

Chitosan Nanoparticles ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Attachment Efficiency ◽

Human Dental Pulp ◽

Pulp Cells ◽

Normal Human

Introduction: Recent in vitro studies have shown that chitosan nanoparticles could enhance the antimicrobial activity of several dental materials. However, the biocompatibility of these nanoparticles with normal human cells is still controversial. The aim of this study was to evaluate the potential toxicity of various sizes and concentrations of chitosan nanoparticles cultured with normal human dental pulp cells. Methods: Normal human dental pulp cells were derived from human dental pulp tissues and cultured with (50-67) nm and (318-350) nm chitosan nanoparticles in concentrations: 0.2 mg/mL, 0.5 mg/mL, 1 mg/mL, and 2 mg/mL as study groups, and 0 mg/mL as a control. The cell attachment efficiency for each group was assessed at 16 hours. The proliferation rate and cell viability were evaluated at days 7 and 14. Both, attachment efficiency and proliferation rate were assessed by measuring the optical density of crystal violet stained cells. The cell viability was determined by the activity of the mitochondrial dehydrogenase enzyme. Statistical analysis was performed using One-Way ANOVA and post hoc Tukey test. Results: All concentrations of the (50-67) nm group significantly reduced cell attachment efficiency in comparison with the control (p<0.01) and with the (318-350) nm group (p<0.01). All concentrations of both groups, (50-67) nm and (318-350) nm, significantly reduced cell proliferation and cell viability compared to the control in dose-dependent and size-associated manners. (p<0.01). Conclusion: Chitosan nanoparticles exhibit a cytotoxic effect on normal human dental pulp cells

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