Chemical Composition and Antibacterial Activity of the Essential Oil of Menthae piperitae L.

The subject of this study was the examination of the chemical composition and antimicrobial activity of peppermint oil (Menthae piperitae L.), that was produced from the plant collected from the plantation in the city of Banja Luka. To examine the chemical composition of the Mentha piperita essential oil we used the GC-FID method. On it’s chromatogram it was clearly shown that the main components of the oil were menthole with the content of 43.66%, menthone 20.02%, iso-menthone 7.73%, following by 1,8-cineole with a percentage of 6.49% and menthil-acetate with 3.31%. The content of the previously mentioned components were all in accordance with the suggestions made by Ph.Eur 8.0. For the purpose of examination antimicrobial activity of the peppermint oil we used agar diffusion method. The results have shown that etheric oil of Menthae piperitae reflects very good antimicrobial activity on all bacterial cultures that we used in this study. The inhibiton zones moved from 12 mm for Pseudomonas aeruginosa to 37.66 mm for Staphylococcus aureus. Such an outcome of our study, and many others from all around the world, indicates the future potential use of peppermint oil in the treatment of bacterial infections, maybe even lowering the use of antibiotics.

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Chemical composition and antimicrobial activity of the essential oil from leaves of Magnolia coriacea (Hung T. Chang & B. L. Chen) figlar growing in Vietnam

ACADEMIA JOURNAL OF BIOLOGY ◽

10.15625/2615-9023/v42n3.14739 ◽

2020 ◽

Vol 42 (3) ◽

Author(s):

Chu Thi Thu Ha ◽

Bui Van Thanh ◽

Dinh Thi Thu Thuy

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Test Microorganism ◽

Leaf Essential Oil ◽

Main Components ◽

Susceptibility Assay

Leaf essential oil of Magnolia coriacea (Hung T. Chang & B. L. Chen) Figlar growing wild in the Bat Dai Son Nature Reserve, Ha Giang Province, Viet Nam was obtained by hydrodistillation and its chemical composition was analyzed using GC/MS. In total, 45 compounds were detected in the essential oil, accounting for 87.1% of the oil, in which 37 compounds were identified accounting for 66.9%. Bicyclogermacrene (12.6%) and spathulenol (17.0%) were the main components of the leaf essential oil of M. coriacea. Antimicrobial activity of the essential oil sample was tested against three microorganism strains using an agar disk diffusion method. The results show that the inhibitory zone diameters ranged from 8.5 to 20.5 mm. Median inhibitory concentration (IC50) and minimum inhibitory concentration (MIC) of the essential oil was determined using microdilution broth susceptibility assay against seven test microorganism strains. Bacillus subtilis had the highest sensitivity with IC50 and MIC values of 185.9 and 512 µg/mL, respectively.

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Chemical Composition and Antimicrobial Activity of the Essential Oil from Flowers of Eryngium triquetrum (Apiaceae) Collected Wild in Sicily

Natural Product Communications ◽

10.1177/1934578x1601100737 ◽

2016 ◽

Vol 11 (7) ◽

pp. 1934578X1601100

Author(s):

Simona Casiglia ◽

Maurizio Bruno ◽

Sergio Rosselli ◽

Felice Senatore

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Art Works ◽

Main Components ◽

Antibacterial And Antifungal ◽

Historical Art

The chemical composition of the essential oil from flowers of Eringium triquetrum Vahl. collected in Sicily was evaluated by GC and GC-MS. The main components were pulegone (50.6%), piperitenone (30.5%) and menthone (7.0%). Comparison of this oil with other studied oils of Eringium species is discussed. The oil showed good antibacterial and antifungal activities against some microorganisms that infest historical art works.

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Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4329899 ◽

2015 ◽

Vol 43 (2) ◽

pp. 432-438 ◽

Cited By ~ 5

Author(s):

Aneta WESOŁOWSKA ◽

Monika GRZESZCZUK ◽

Dorota JADCZAK ◽

Paweł NAWROTEK ◽

Magdalena STRUK

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Micrococcus Luteus ◽

Gas Chromatography Mass Spectrometry ◽

E Coli ◽

Thymus Serpyllum ◽

Main Components ◽

Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

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In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽

10.3390/plants9040453 ◽

2020 ◽

Vol 9 (4) ◽

pp. 453 ◽

Cited By ~ 8

Author(s):

Nhan Trong Le ◽

Duc Viet Ho ◽

Tuan Quoc Doan ◽

Anh Tuan Le ◽

Ain Raal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Antiviral Effects ◽

Scientific Report ◽

Main Components ◽

Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

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Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

Natural Product Communications ◽

10.1177/1934578x0700200517 ◽

2007 ◽

Vol 2 (5) ◽

pp. 1934578X0700200

Author(s):

Fadwa El Hanbali ◽

Ahmed El Hakmaoui ◽

Fouad Mellouki ◽

Lahoussine El Rhaffari ◽

Mohamed Akssira

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

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Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

Natural Product Communications ◽

10.1177/1934578x1200700934 ◽

2012 ◽

Vol 7 (9) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Megil J. McNeil ◽

Roy B. R. Porter ◽

Lawrence A. D. Williams

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Oil Composition ◽

Filter Paper Disc ◽

Aerial Parts ◽

Main Components ◽

Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

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Composition and Antimicrobial Activity ofEuphrasia rostkovianaHayne Essential Oil

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/734101 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 13

Author(s):

Pavel Novy ◽

Hana Davidova ◽

Cecilia Suqued Serrano-Rojero ◽

Johana Rondevaldova ◽

Josef Pulkrabek ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Bacterial Infections ◽

Antimicrobial Effect ◽

Main Constituent ◽

Hexadecanoic Acid ◽

Eye Infections ◽

Gram Positive Bacteria ◽

Health Disorders

Eyebright,Euphrasia rostkovianaHayne (Scrophulariaceae), is a medicinal plant traditionally used in Europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. SomeEuphrasiaspecies have been previously reported to contain essential oil. However, the composition and bioactivity ofE. rostkovianaoil are unknown. Therefore, in this study, we investigated the chemical composition and antimicrobial activity of the eyebright essential oil against some organisms associated with eye infections:Enterococcus faecalis,Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,S. epidermidis,Pseudomonas aeruginosa, andCandida albicans. GC-MS analysis revealed more than 70 constituents, with n-hexadecanoic acid (18.47%) as the main constituent followed by thymol (7.97%), myristic acid (4.71%), linalool (4.65%), and anethole (4.09%). The essential oil showed antimicrobial effect against all organisms tested with the exception ofP. aeruginosa. The best activity was observed against all Gram-positive bacteria tested with the minimum inhibitory concentrations of 512 µg/mL. This is the first report on the chemical composition ofE. rostkovianaessential oil and its antimicrobial activity.

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Chemical Composition and Antimicrobial Activity of Rosmarinus officinalis L. Essential Oil Obtained via Supercritical Fluid Extraction

Journal of Food Protection ◽

10.4315/0362-028x-68.4.790 ◽

2005 ◽

Vol 68 (4) ◽

pp. 790-795 ◽

Cited By ~ 117

Author(s):

S. SANTOYO ◽

S. CAVERO ◽

L. JAIME ◽

E. IBAÑEZ ◽

F. J. SEÑORÁNS ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Rosmarinus Officinalis ◽

Extraction Temperature ◽

Gas Chromatography Mass Spectroscopy ◽

The One ◽

Main Components ◽

Rosmarinus Officinalis L ◽

Total Oil

The chemical composition and antimicrobial activity of essential oil–rich fractions obtained by supercritical CO2 extraction from Rosmarinus officinalis L. were investigated. Gas chromatography–mass spectroscopy analysis of these fractions resulted in the identification of 33 compounds of the essential oil. The main components of these fractions were α-pinene, 1,8-cineole, camphor, verbenone, and borneol, constituting ca. 80% of the total oil. The antimicrobial activity was investigated by the disc diffusion and broth dilution methods against six microbial species, including gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), a yeast (Candida albicans), and a fungus (Aspergillus niger). All of the essential oil–rich fractions obtained showed antimicrobial activity against all of the microorganisms tested, with inhibition zones and minimal bactericidal and fungicidal concentration values in the range of 17 to 33 mm and 2.25 to 0.25 mg/ml, respectively. The most active fraction was the one obtained in experiment 4 (4% ethanol as modifier; extraction pressure, 25 MPa; extraction temperature, 60°C). S. aureus was found to be the most sensitive bacteria to the rosemary extracts, whereas the least susceptible was A. niger. α-Pinene, 1,8-cineole, camphor, verbenone, and borneol standards also showed antimicrobial activity against all the microorganisms tested, borneol being the most effective followed by camphor and verbenone. In that way, it was confirmed that essential oil from experiment 4, with the best antimicrobial activity, presented the highest quantity of camphor, borneol, and verbenone.

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Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201012 ◽

2019 ◽

Vol 20 (10) ◽

Author(s):

Souheila Bounab ◽

Takia Lograda ◽

Messaoud Ramdani ◽

Pierre Chalard ◽

Gilles Figueredo

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Diffusion Method ◽

Nonanoic Acid ◽

Upgma Cluster Analysis ◽

Oil Concentration ◽

Thymelaea Hirsuta ◽

Main Components

Abstract. Souhila B, Takia L, Messaoud R, Pierre C, Gilles F. 2019. Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria. Biodiversitas 20: 2868-2876. The objectives of this study were to determine the chemical composition and to evaluate the antibacterial activity of Thymelaea hirsuta (L.) Endl., essential oils from seven sampling locations in M'sila region (Algeria). Extraction of essential oils was carried out by the hydro-distillation; the analysis of chemical composition of essential oil was carried out by GC-MS. Antimicrobial activity was performed by disc diffusion method at the essential oil concentration of non-diluted and diluted (1:2, 1:4 and 1:8 v:v of DMSO) against eight species of bacteria. The results showed that the average yields of essential oils were 0.3 ± 0.12%. A total of 45 components were identified, averaging 98.2 ± 1.85% of the total oils. The main components were nonanal-n (10.39 ± 3.21%), hexadecanoic acid (9.77 ± 2.81%), nonanoic acid (9.13 ± 6.49%), triacontane (7.2 ± 3.34%), isopropyl tetradecanoate (6.16 ± 1.99%) and tridecane (4.87 ± 3.1%). Based on the UPGMA cluster analysis, there were two clades of T. hirsuta. T. hirusta has a chemical polymorphism with different chemotypes marked in nature. There were four chemotypes identified in the essential oil of T. hirsuta in the region of M'sila. The essential oil of T. hirsuta has antibacterial activity against eight tested bacteria on the concentration-dependentt manner.

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Chemical composition and antimicrobial activity of extracts from thyme and rosemary against Staphylococcus aureus and Candida albicans

BioResources ◽

10.15376/biores.15.4.9656-9671 ◽

2020 ◽

Vol 15 (4) ◽

pp. 9656-9671

Author(s):

Akram Sabzikar ◽

Seyyed Khalil Hosseinihashemi ◽

Younes Shirmohammadli ◽

Abbas Jalaligoldeh

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Inhibition Zone ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Rosemary Extract ◽

Gram Positive Bacteria ◽

Main Components

Different concentrations of ethanolic extracts of thyme (Zataria multiflora) and rosemary (Rosmarinus officinalis) were evaluated to determine their antimicrobial activity using the agar-well diffusion method. The values of inhibition zone diameter (IZD) for Candida albicans fungus and Staphylococcus aureus Gram-positive bacteria were determined. The bioactivities of two various extracts were studied, and the chemical composition of the extracts were identified using gas chromatography-mass spectrometry (GC-MS) technique. The results of the test showed that at concentrations of 10% and 40% thyme extract, the values of IZD were 12.5 mm and 23.3 mm, respectively, against the growth of S. aureus, which were higher than C. albicans (7.0 mm and 22.5 mm, respectively). The rosemary extract at concentrations of 20% and 60% showed lower antibacterial activity against S. aureus (4.7 mm and 8.7 mm IZD, respectively) and lower antifungal activity against C. albicans (12.2 mm and 1.7 mm IZD, respectively). At a concentration of 40% thyme extract, the highest antibacterial (23.3 mm IZD) and antifungal (22.5 mm IZD) activities were observed. The GC/MS analysis showed that carvacrol (52.3%), linalool L (16%), and thymol (9.6%) were the main components of thyme extract, while in the rosemary extract β-amyrone (18.0%), verbenone (8.0%), and 1,8-cineole (7.26%) were the major constituents.

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