scholarly journals Whole genome sequence analysis of multi drug resistant community associated methicillin resistant Staphylococcus aureus from food fish: detection of clonal lineage ST 28 and its antimicrobial resistance and virulence genes

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11224
Author(s):  
Gopalan Krishnan Sivaraman ◽  
Visnuvinayagam Sivam ◽  
Balasubramanian Ganesh ◽  
Ravikrishnan Elangovan ◽  
Ardhra Vijayan ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genome Sequence ◽  
Virulence Genes ◽  
Draft Genome ◽  
Whole Genome Sequence ◽  
Comparative Genomic ◽  
Drug Resistant ◽  
Methicillin Resistant ◽  
Antimicrobial Resistance Genes ◽  
Food Fish

Methicillin-resistant staphylococcus aureus (MRSA) sequence type 28 (ST 28) and spa type t021 is a CC30, prototype of ST-30, Community Associated-MRSA (CA-MRSA) (lukS-lukF +). It is a multi-drug resistant strain harbouring staphylococcal endotoxins, haemolysins, ureolysin, serine protease, and antimicrobial resistance genes. In this study, we report the draft genome sequence of this MRSA isolated from the most commonly used food fish, ribbon fish (Trichiurus lepturus). The total number of assembled paired-end high-quality reads was 7,731,542 with a total length of  2.8Mb of 2797 predicted genes. The unique ST28/ t021 CA- MRSA in fish is the first report from India, and in addition to antibiotic resistance is known to co-harbour virulence genes, haemolysins, aureolysins and endotoxins. Comprehensive comparative genomic analysis of CA-MRSA strain7 can help further understand their diversity, genetic structure, diversity and a high degree of virulence to aid in fisheries management.

scholarly journals Genome Sequence of a Unique t2247-ST692-III Livestock-Associated Methicillin-Resistant Staphylococcus aureus Strain from Chicken Carcass

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Dong Chan Moon ◽  
Byung-Yong Kim ◽  
Migma Dorji Tamang ◽  
Hyang-Mi Nam ◽  
Geum-Chan Jang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Genome Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Draft Genome ◽  
Aureus Strain ◽  
Methicillin Resistant ◽  
Chicken Carcass ◽  
A Genome ◽  
Antimicrobial Resistance Genes

We report the draft genome sequence of a novel livestock-associated t2247-ST692-III methicillin-resistant Staphylococcus aureus strain designated K12S0375, which was isolated from a chicken carcass in South Korea. The K12S0375 strain contains uncommon genes, including antimicrobial resistance genes ( tetL and tetS ) and leukotoxin ( lukED ), and the genomic distance indicates a single lineage in a genome-based phylogenetic tree compared with 459 S. aureus genome sequences. This genome sequence will contribute to understanding epidemiological and genomic features of the ST692 lineage, including antimicrobial resistance and virulence genes.

scholarly journals Draft Genome Sequence of Methicillin-Resistant Staphylococcus aureus Harboring Staphylococcal Cassette Chromosome mec Type IX, Isolated from a Fatal Bacteremic Pneumonia Case

2021 ◽  
Vol 10 (30) ◽  
Author(s):  
Peechanika Chopjitt ◽  
Thidathip Wongsurawat ◽  
Piroon Jenjaroenpun ◽  
Parichart Boueroy ◽  
Watcharaporn Kamjumphol ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Whole Genome Sequence ◽  
Methicillin Resistant ◽  
Content Type ◽  
Bacteremic Pneumonia ◽  
Fatal Sepsis ◽  
Staphylococcal Cassette Chromosome

Here, we report the whole-genome sequence of a methicillin-resistant Staphylococcus aureus strain harboring staphylococcal cassette chromosome mec (SCC mec ) type IX, isolated from a fatal sepsis case. Genomic analysis revealed that the isolate was sequence type 9 and spa type t3446, carrying multiple antimicrobial-resistant genes comprising mecA , blaZ , aac(6′)-aph(2″) , aadD , ant(6)-Ia , lsa (E), dfrG , tet (M), fexA , and lnu (B).

scholarly journals Draft Genome Sequence of Enterobacter hormaechei subsp. steigerwaltii Strain BEI01

2021 ◽  
Vol 10 (28) ◽  
Author(s):  
Soon Keong Wee ◽  
Sharon Cui Mun Chan ◽  
Xue Li Guan ◽  
Eric Peng Huat Yap
Keyword(s):  
Antimicrobial Resistance ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Draft Genome ◽  
Gc Content ◽  
Enterobacter Cloacae ◽  
Metal Resistance ◽  
Content Type ◽  
Antimicrobial Resistance Genes ◽  
Enterobacter Hormaechei

Here, we report the genome sequence of Enterobacter hormaechei subsp. steigerwaltii strain BEI01, originally deposited as a member of the Enterobacter cloacae complex. The genome is 4,900,246 bp in size with a GC content of 55.44%; it contains multidrug antimicrobial resistance genes and several metal resistance gene operons.

scholarly journals Whole Genome Sequencing of Staphylococci Isolated From Bovine Milk Samples

2021 ◽  
Vol 12 ◽  
Author(s):  
Marte Ekeland Fergestad ◽  
Fabrice Touzain ◽  
Sarne De Vliegher ◽  
Anneleen De Visscher ◽  
Damien Thiry ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Virulence Genes ◽  
Bovine Milk ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Bacterial Survival ◽  
Intramammary Infections ◽  
Antimicrobial Resistance Genes

Staphylococci are among the commonly isolated bacteria from intramammary infections in bovines, where Staphylococcus aureus is the most studied species. This species carries a variety of virulence genes, contributing to bacterial survival and spread. Less is known about non-aureus staphylococci (NAS) and their range of virulence genes and mechanisms, but they are the most frequently isolated bacteria from bovine milk. Staphylococci can also carry a range of antimicrobial resistance genes, complicating treatment of the infections they cause. We used Illumina sequencing to whole genome sequence 93 staphylococcal isolates selected from a collection of staphylococcal isolates; 45 S. aureus isolates and 48 NAS isolates from 16 different species, determining their content of antimicrobial resistance genes and virulence genes. Antimicrobial resistance genes were frequently observed in the NAS species as a group compared to S. aureus. However, the lincosamide resistance gene lnuA and penicillin resistance gene blaZ were frequently identified in NAS, as well as a small number of S. aureus. The erm genes conferring macrolide resistance were also identified in several NAS isolates and in a small number of S. aureus isolates. In most S. aureus isolates, no antimicrobial resistance genes were detected, but in five S. aureus isolates three to six resistance genes were identified and all five of these carried the mecA gene. Virulence genes were more frequently identified in S. aureus, which contained on average five times more virulence genes compared to NAS. Among the NAS species there were also differences in content of virulence genes, such as S. chromogenes with a higher average number of virulence genes. By determining the content of a large selection of virulence genes and antimicrobial resistance genes in S. aureus and 16 different NAS species our results contribute with knowledge regarding the genetic basis for virulence and antimicrobial resistance in bovine staphylococci, especially the less studied NAS. The results can create a broader basis for further research into the virulence mechanisms of this important group of bacteria in bovine intramammary infections.

scholarly journals Draft Genome Sequence of a Methicillin-Resistant Staphylococcus aureus Isolate (Sequence Type 1) from Seafood

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
G. K. Sivaraman ◽  
Deesha Vanik ◽  
S. Visnuvinayagam ◽  
M. M. Prasad ◽  
C. N. Ravishankar
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Whole Genome Sequence ◽  
Protein Coding ◽  
Methicillin Resistant ◽  
Content Type

ABSTRACT The draft genome sequence of a methicillin-resistant Staphylococcus aureus (MRSA) isolate (sequence type 1 [ST 1]) from the salted dried ribbonfish from Gujarat, India, is reported here. Staphylococcus genus-specific genes were present in this MRSA isolate. The whole-genome sequence of this strain contains 2,797 protein-coding genes and 80 RNAs within the 2.85-Mb genome.

scholarly journals Draft Genome Sequences of 50 Methicillin-Resistant Staphylococcus aureus Sequence Type 5 Isolates Obtained from a U.S. Hospital

2017 ◽  
Vol 5 (44) ◽  
Author(s):  
Samantha J. Hau ◽  
Darrell O. Bayles ◽  
David P. Alt ◽  
Tracy L. Nicholson
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Draft Genome ◽  
Sequence Type ◽  
Genome Sequences ◽  
Methicillin Resistant ◽  
Content Type ◽  
Related Disease ◽  
Antimicrobial Resistance Genes

ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) can be a commensal or pathogen in humans. Pathogenicity and disease are related to the acquisition of mobile genetic elements encoding virulence and antimicrobial resistance genes. Here, we report draft genome sequences for 50 clinical MRSA isolates from humans with MRSA-related disease.

scholarly journals Draft Genome Sequence of a Methicillin-Resistant Sequence Type 39 Staphylococcal Isolate Obtained from Seafood

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
G. K. Sivaraman ◽  
Deesha Vanik ◽  
S. Visnuvinayagam ◽  
Mothadaka Mukteswar Prasad ◽  
V. Murugadas ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Whole Genome Sequence ◽  
Protein Coding ◽  
Methicillin Resistant ◽  
Content Type ◽  
Protein Coding Genes ◽  
Staphylococcal Isolate

ABSTRACT The draft genome sequence of a methicillin-resistant Staphylococcus aureus (MRSA) sequence type 39 (ST 39) isolate obtained from the dried ribbonfish of Gujarat, India, is reported here. Staphylococcus-specific genes were present in this MRSA isolate. The whole-genome sequence of this strain contains 2,693 protein-coding genes and 70 RNAs within the 2.82-Mb genome.

Identification of Antibiotic Resistance Genes in Multi-Drug Resistant Acinetobacter Baumannii Clinical Isolates of Iraqi Patients (Zq Strains), Using Whole-Genome Sequencing

2019 ◽  
Vol 10 (04) ◽  
pp. 670-680
Author(s):  
Zainab J Qasim ◽  
Haider Sabah Kadhim ◽  
Ahmed Sahib Abdulamir
Keyword(s):  
Antimicrobial Resistance ◽  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Genetic Material ◽  
Whole Genome ◽  
Drug Resistant ◽  
Intrinsic Resistance ◽  
Antimicrobial Resistance Genes ◽  
Recent Emergence

Background: The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii (A. baumannii ) has raised concern in health care settings in Iraq. This is the first report of the whole genome sequence of A. baumannii ZQ isolated from Iraqi patients. To better comprehend the repertoire of MDR genetic elements and organization, we compared the genome sequences of eight extended drug-resistant (XDR) and two less drug-resistant A. baumannii ZQ strains with that of other completely sequenced A. baumannii from divergent worldwide distributed isolates.Results: In consistence with their phenotypic antimicrobial resistance profiles, ZQ genomes harbors high to moderate numbers of genetic determinants, including β-lactamases, aminoglycoside-modifying enzymes, efflux pumps, modifications of target sites. Several strains showed nearly identical genome sequence, frequent structural variation was detected even between the closely related strains.Conclusion: In general, the shorter the genetic distance among strains, the less insertion/deletion events proceed. However, frequent genomic changes was observed even inside the closely related strains of A. baumannii. Antimicrobial resistance genes are likely to be the target accumulating such variations, suggesting that the resistance elements respond actively to the selection pressure in the hospital setting. Besides the lateral acquisition of genetic material from resistant bacterial strains, the drastic issues is associated with continuous presence of intrinsic resistance genes in the genome of A. baumannii, which are ready to be boosted by exposure to sub-inhibitory levels of the antibiotics in the environment and might also play an important role in the evolution of resistance to the new derivatives of different antibiotic classes.

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