scholarly journals Enhanced soluble sugar content in tomato fruit using CRISPR/Cas9-mediated SlINVINH1 and SlVPE5 gene editing

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12478
Author(s):  
Baike Wang ◽  
Ning Li ◽  
Shaoyong Huang ◽  
Jiahui Hu ◽  
Qiang Wang ◽  
...  
Keyword(s):  
Gene Editing ◽  
Single Gene ◽  
Tomato Fruit ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Wild Type ◽  
Tomato Fruits ◽  
Knock Out ◽  
Soluble Sugar Content ◽  
Soluble Solid

Soluble sugar is known to improve the sweetness and increase tomato sauce yield. Studies have focused on improving the content of soluble sugar in tomato fruits, usually by promoting functional genes. We studied two genes (SlINVINH1 and SlVPE5) that inhibited the accumulation of soluble sugar in tomato fruits and obtained two genes’ knocked-out lines (CRISPR-invinh1 or CRISPR-vpe5) using CRISPR/Cas9. Aggregated lines with CRISPR-invinh1 and CRISPR-vpe5 were gained by hybridization and self-pollination. Compared to wild-type lines, the glucose, fructose, and total soluble solid (TSS) contents of CRISPR-invinh1 and CRISPR-vpe5 increased significantly. Glucose, fructose, and TSS levels further improved simultaneously with CRISPR-invinh1 and CRISPR-vpe5 than with single gene knock-out lines. This indicates that these genes have a synergistic effect and will increase the soluble sugar content. Thus, the knock-out SlINVINH1 and SlVPE5 may provide a practical basis for improving the sweetness of tomato fruits and their processing quality.

Genetic characteristics and QTL analysis of the soluble sugar content in ripe tomato fruits

2021 ◽  
Vol 276 ◽  
pp. 109785
Author(s):  
Yao Tang ◽  
Jing Ren ◽  
Chunxin Liu ◽  
Jingbin Jiang ◽  
Huanhuan Yang ◽  
...  
Keyword(s):  
Qtl Analysis ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Tomato Fruits ◽  
Genetic Characteristics ◽  
Soluble Sugar Content ◽  
Ripe Tomato

scholarly journals Creating Amylose-Free Barley Cultivars with High Soluble Sugar Content By Genome Editing

2021 ◽  
Author(s):  
Yun Li ◽  
Yanyan Jiang ◽  
Shiting Fan ◽  
Xiaolong Gan ◽  
Dong Cao ◽  
...  
Keyword(s):  
Genome Editing ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Wild Type ◽  
Waxy Protein ◽  
Soluble Sugar Content ◽  
Barley Cultivars ◽  
Novel Alleles ◽  
Amylose Free

Abstract Background Amylose biosynthesis is strictly associated with granule-bound starch synthase I (GBSSI) encoded by the Waxy gene. Waxy barley has extensive prospects for application in functional food development and the brewing industry; however, amylose-free waxy barleys are relatively scarce in nature. Results Here we created new alleles of the Waxy gene using CRISPR/Cas9 genome editing. Mutagenesis of single bases in these novel alleles caused absence of intact waxy protein in grain of the edited line. Consequently, B-type granules disappeared. The amylose and amylopectin contents of the edited line were zero and 31.73%, while those in the wild type (WT) were 33.50% and 39.00%, respectively. The absence of waxy protein led to increase in soluble sugar content to 37.30% compared with only 10.0% in the WT. Typical soluble sugars, sucrose and β-glucan, were 39.16% and 35.40% higher in the edited line than in the WT, respectively. Transcriptome analysis identified differences between the edited line and the WT that could partly explain the reduction in amylose and amylopectin contents and the increase in soluble sugar, sucrose and β-glucan contents. Conclusions The barley cultivar with novel alleles of the Waxy gene contained zero amylose, lower amylopectin, and higher soluble sugar, sucrose and β-glucan than the wild type. This new cultivar provides a good germplasm resource for improving the quality of barley.

The Influence of Chlorophenoxy Herbicides MCPA on Creeping Bentgrass Physiological Index

2011 ◽  
Vol 356-360 ◽  
pp. 2763-2766 ◽  
Author(s):  
Yang Fan Gao ◽  
Ming Wang Shi ◽  
Jian Hua Wang
Keyword(s):  
Chlorophyll Content ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Creeping Bentgrass ◽  
Physiological Index ◽  
Soluble Sugar Content ◽  
Content Determination ◽  
Chlorophenoxy Herbicides ◽  
Test Result

In this article, we studied to different concentrations of MCPA to creeping bentgrass Growth. Through the creeping bentgrass in four different periods of chlorophyll content, MDA and soluble sugar content determination. This test result showed:With the MCPA concentration increases, creeping bentgrass decline of chlorophyll content in the same period of growth, MDA and soluble sugar content increased. MCPA used after the early pair of creeping bentgrass growth is large, to put off with MCPA handle time, to affect to creeping bentgrass growth is gradually decreased.

scholarly journals Inactivation of porcine interleukin-1β results in failure of rapid conceptus elongation

2017 ◽  
Vol 115 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Jeffrey J. Whyte ◽  
Ashley E. Meyer ◽  
Lee D. Spate ◽  
Joshua A. Benne ◽  
Raissa Cecil ◽  
...  
Keyword(s):  
Gene Editing ◽  
Mammalian Species ◽  
Interleukin 1Β ◽  
Corpora Lutea ◽  
Morphological Transition ◽  
Wild Type ◽  
Morphological Transformation ◽  
Proinflammatory Response ◽  
Knock Out ◽  
Estrogen Synthesis

Conceptus expansion throughout the uterus of mammalian species with a noninvasive epitheliochorial type of placentation is critical establishing an adequate uterine surface area for nutrient support during gestation. Pig conceptuses undergo a unique rapid morphological transformation to elongate into filamentous threads within 1 h, which provides the uterine surface to support development and maintain functional corpora lutea through the production of estrogen. Conceptus production of a unique interleukin 1β, IL1B2, temporally increases during the period of trophoblast remodeling during elongation. CRISPR/Cas9 gene editing was used to knock out pig conceptus IL1B2 expression and the secretion of IL1B2 during the time of conceptus elongation. Trophoblast elongation occurred on day 14 in wild-type (IL1B2+/+) conceptuses but did not occur in ILB2-null (IL1B2−/−) conceptuses. Although the morphological transition of IL1B2−/− conceptuses was inhibited, expression of a number of conceptus developmental genes was not altered. However, conceptus aromatase expression and estrogen secretion were decreased, indicating that IL1B2 may be involved in the spatiotemporal increase in conceptus estrogen synthesis needed for the establishment of pregnancy in the pig and may serve to regulate the proinflammatory response of endometrium to IL1B2 during conceptus elongation and attachment to the uterine surface.

scholarly journals A Long-Day Photoperiod and 6-Benzyladenine Promote Runner Formation through Upregulation of Soluble Sugar Content in Strawberry

2020 ◽  
Vol 21 (14) ◽  
pp. 4917
Author(s):  
Yali Li ◽  
Jiangtao Hu ◽  
Hao Wei ◽  
Byoung Ryong Jeong
Keyword(s):  
Molecular Mechanisms ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Orthogonal Test ◽  
Influential Factor ◽  
Asexual Propagation ◽  
Soluble Sugar Content ◽  
Long Day ◽  
Seed Propagation ◽  
Dimensional Electrophoresis

Commercial strawberries are mainly propagated using daughter plants produced on aerial runners because asexual propagation is faster than seed propagation, and daughter plants retain the characteristics of the mother plant. This study was conducted to investigate the effective factors for runner induction, as well as the molecular mechanisms behind the runner induction. An orthogonal test with 4 factors (photoperiod, temperature, gibberellin, and 6-benzyladenine), each with 3 levels was performed. Proteins were also extracted from the crowns with or without runners and separated by two-dimensional electrophoresis. The results of the orthogonal test showed that a long-day (LD) environment was the most influential factor for the runner formation, and 50 mg·L−1 of 6-BA significantly increased the number of runners. A proteomic analysis revealed that 32 proteins were differentially expressed (2-fold, p < 0.05) in the strawberry crowns with and without runners. A total of 16 spots were up-regulated in the crowns with runners induced by LD treatment. Identified proteins were classified into seven groups according to their biological roles. The most prominent groups were carbohydrate metabolism and photosynthesis, which indicated that the carbohydrate content may increase during runner formation. A further analysis demonstrated that the soluble sugar content was positively correlated with the number of runners. Thus, it is suggested that the photoperiod and 6-BA break the dormancy of the axillary buds and produce runners by increasing the soluble sugar content in strawberry.

scholarly journals Effects of Biochar on Soluble Sugar Content in Peach Seedlings

2019 ◽  
Vol 136 ◽  
pp. 07010
Author(s):  
Jing Sun ◽  
Zhiyu Li ◽  
Jinpeng Zhu ◽  
Yuxi Wang ◽  
Tonghao Cui ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Plant Height ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Stem Diameter ◽  
Corn Straw ◽  
Paddy Straw ◽  
Soluble Sugar Content ◽  
Rape Straw ◽  
Significant Difference

A pot experiment was conducted to study the effects of different biochar types [addition of rape straw biochar (RB), addition of paddy straw biochar (PB), addition of wheat straw biochar (WB) and addition of corn straw biochar (MB)] on the soluble sugar content and plant height of peach seedlings. The result showed that the soluble sugar content in roots and leaves of peach seedlings treated with biochar was lower than the control. However, the soluble sugar content in stems was higher than control compared with the control. In the soluble sugar content in the aboveground part, there was no significant difference between the treatment of adding biochar and the control except for the treatment of PB. All the treatment of adding biochar increased the plant height of peach seedlings except the treatment of MB than the control and the treatment of RB and PB were at a higher level. Compared to control, all biochar addition treatments increased stem diameter of peach seedlings and the treatment of RB and PB were at a higher level. Therefore, the treatment of RB and PB was beneficial to the growth of peach seedlings.

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