scholarly journals Fatty Acid Compositions of Mixed Microalgae from Tilapia Fish Ponds

2019 ◽  
pp. 1-7
Author(s):  
Norhafizah Osman ◽  
Hishamuddin Omar
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Acid Methyl Ester ◽  
Monounsaturated Fatty Acids ◽  
Major Constituent ◽  
Fish Ponds ◽  
Fatty Acid Profiles ◽  
Fatty Acid Compositions

Microalgae has been getting broad attention of researchers and investors lately, especially when discussing on healthy food and energy sources for the future. In this study, twelve samples of mixed microalgae from outdoor ponds were analyzed for their fatty acid compositions. The potential of microalgae to solve variety of world’s problems was not realized because of bottleneck in microalgal supplies at reasonable cost. Therefore, the objective of this study is to determine fatty acid profiles of mixed microalgae from tilapia fish ponds. The study was conducted in Tapak Ternakan Ikan, Taman Pertanian Universiti and Department of Biology, Faculty of Science, Universiti Putra Malaysia. Mixed microalgae were extracted for their lipids with methanol: chloroform mixture and after transesterification, the fatty acid methyl ester were analyzed using gas chromatography equipped with flame ionization detector. Results showed that saturated was the major constituent fatty acids. The average percentages of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids obtained were 45.62 ± 1.37%, 20.05 ± 1.14%, and 34.33 ± 3.17% respectively. The most dominant fatty acid profiles were C18:3n3 (α-linolenic acid) and C16:0 (palmitic acid), with the overall percentages of 19.97% and 19.40% respectively. The fatty acid profiles of mixed microalgae was good with a decent balance of saturated, monounsaturated and polyunsaturated fatty acids.

scholarly journals Protein, amino acid and fatty acid profiles of two edible crabs of Lagos coast,Nigeria

2019 ◽  
Vol 17 (3) ◽  
pp. 396-401
Author(s):  
Rasheed Olatunji Moruf ◽  
Aderonke Omolara Lawal-Are
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Amino Acid ◽  
Crude Protein ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Essential Amino Acids ◽  
Fatty Acid Profiles ◽  
Protein Amino Acid ◽  
Fatty Acid Compositions

Edible crabs constitute one of the major sources of nutritious food for human nutrition. The study was aimed to examine the protein, amino acid and fatty acid compositions of two edible crabs (Callinectes amnicola and Portunus validus) of Lagos coast, Nigeria. Samples were obtained from the water body, transported in crushed iced insulated containers and analyzed in the laboratory using standard methods. Crude protein varied from 55 - 68.92% (C. amnicola) and 51.9 - 67.51% (P. validus) on dry matter basis. Leucine (8.63 - 9.73g/100 g crude protein) and glutamic acid (12.71 - 16.19g/100 g crude protein) were the highest concentrated essential and non-essential amino acids, respectively. The coefficient of variation of the amino acid values indicated that C. amnicola has more variations, relative to its mean. The most concentrated saturated fatty acid was palmitic acid in the samples with significantly different (P <0.05) values of 14.32±1.05% (C. amnicola) and 24.52±0.00% (P. validus). With the exception of lauric, arachidic and lignoceric, all saturated fatty acids were more concentrated in P. validus than in C. amnicola. Oleic acid among the monounsaturated fatty acids occupied the highest position in C. amnicola (17.6233±0.04%) and in P. validus (12.4682±0.25%); whereas timnodenic acid was the most concentrated among the polyunsaturated fatty acids, with values of 23.9744±0.48 % (C. amnicola) and 15.7234±0.25 % (P. validus). Both species have reasonable levels of dietary protein and lipid quality. J Bangladesh Agril Univ 17(3): 396–401, 2019

scholarly journals Analysis of Fatty Acids in Breastmilk During a 9-month Lactation Period Using Gas Chromatography–Mass Spectroscopy (GC-MS)

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Titin Aryani ◽  
Dwi Ernawati ◽  
Henny Parida Hutapea
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Content ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Acid Methyl Ester ◽  
Monounsaturated Fatty Acids ◽  
Lactation Period ◽  
Omega 6

Breastmilk is very much needed to meet the needs of babies in every way because it contains important nutrients, carbohydrates in the form of lactose, and fatty acids in the form of polyunsaturated fatty acids. Research on fatty acid content in breastmilk with a lactation period of 9 months is needed to provide information to the public related to fatty acid content of breastmilk during 9 months lactation. The study is to analyze fatty acid content in the form of saturated fatty acids or unsaturated fat contained in breast milk. Breastmilk taken in the study was as much as 50 ml in a 250 ml erlenmeyer solution. To the sample 50 ml of concentrated, HCL concentration was added as much as 5 m and then the solution was beat for 15 minutes and was exposed ultrasonic therapy for 1 hour. The solution was then extracted using n-hexane and aquabidest solution until the milk oil solution was separated. Milk oil was extracted by adding 50 ml of Na2SO4 1 m then deciphere was performed. The breastmilk milk extraction oil was weighed 0.1 gram in a Teflon-filled reaction tube. A 15% BF3 solution in methanol was added 0.5 ml and then was heated in an air bath with a temperature of 45 ° C for 30 minutes. After cooling, solution of 0.2 ml of n-hexane was added to form two layers. The upper layer which was fatty acid methyl ester was taken by using syringe and then was injected in GC-MS. In this study, the total saturated fatty acid was 42.54% and the total unsaturated fatty acid was 57.17%. Saturated fatty acids consisted of lauric, myristic, palmitate, and stearate. Omega-6 fatty acids and monounsaturated fatty acids consisted of omega-9 fatty acids. In Mother's Milk with 9 months lactation period there are various fatty acid content of both saturated fatty acids, monounsaturated fatty acids (omega-9) and polyunsaturated fatty acids (omega-6).

scholarly journals Systematic Evaluation of Fatty Acid Profiles in Hydrachna processifera, Eylais setosaand Hydrodroma despiciens (Acari, Hydrachnidia) Species by GC-MS Method

2019 ◽  
pp. 46-50
Author(s):  
Ferruh Aşçi ◽  
Mehmet İnak İnak ◽  
Sait Bulut
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Molecular Taxonomy ◽  
Monounsaturated Fatty Acids ◽  
Mite Species ◽  
Systematic Evaluation ◽  
Fatty Acid Profiles ◽  
Significant Difference

This study was carried out with Hydrachna processifera (Acari, Hydracnidia), Eylais setosa and Hydrodroma despiciens common in lakes. Fatty acid ratios were evaluated comparatively in terms of species. For this purpose, these species collected from the Karamık lake (AfyonkarahisarTurkey) were analyzed by GC-MS gas chromatography in the laboratory. In the results of the analysis, saturated fatty acids such as myristic (C14:0), palmitic (C16:0), heptadecanoic (C17:0), and stearic acid (C18:0) were observed in high amount. There was a significant difference between species in terms of total saturated fatty acid ratios. The monounsaturated fatty acids palmitoleic (C16:1), oleic (C18:1), and erucic (C22:1) are the most common acids. The major polyunsaturated fatty acids were linolenic (C18:3), eicosatrienoic (C20:3), eicosapentaenoic (C20:5) and docosahexaenoic (C22:6) acid, linoleic (C18:2), gamma-linolenic 3), eicosadienoic (C20:2) and arachidonic (C20:4) acids. At the end of the study, there were considerable differences between the species studied in terms of monounsaturated and polyunsaturated fatty acids in these water mite species. This study also found that the fatty acid composition was different for each species and this is important for the molecular taxonomy of water mites.

scholarly journals Effects of polyunsaturated fatty acids in diets fed to sows on fatty acids in brain, muscle and skin of their piglets

2020 ◽  
Vol 50 (1) ◽  
pp. 47-54
Author(s):  
I De Gasperín ◽  
J.G. Vicente ◽  
J.M. Pinos-Rodríguez ◽  
F Montiel ◽  
R Loeza ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Soybean Oil ◽  
Fatty Acid Profile ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Fatty Acid Profiles ◽  
Polyunsaturated Fat ◽  
Pork Lard ◽  
Piglet Survival

The aim of this research was to determine fatty acid profiles in piglet brain, skin, and muscle, and in the milk of sows fed fat with different saturation grades during gestation and lactation. At 42 days of gestation, 50 multiparous sows were randomly allocated to one of two treatments, namely a diet containing pork lard (n = 25) and a diet containing soybean oil (n = 25). The fats were provided at 3.6% during gestation and at 4% during lactation. The experimental diets were offered through the weaning of the piglets. The fatty acid profile of the milk was determined fourteen days after parturition. At weaning (21 days postpartum) and seven days later, one of the piglets (n = 64) from 16 sows allocated to each treatment was selected at random to determine fatty acid profiles in brain, skin and muscle. Saturated and monounsaturated fatty acids were higher in the diet with pork lard than in that with soybean oil, in which the polyunsaturated fat content was higher. A higher saturation of fatty acids was found in milk from the sows that consumed pork lard, which contained more saturated fatty acids than the milk from sows that consumed soybean oil. The fatty acid profiles in muscle and skin of the piglets were affected by the diet of the sows. However, the fatty acid profile of the piglets’ brains was not affected by the diet of their mothers. Keywords: fat saturation, lard, piglet survival, sow feeding, soybean oil

scholarly journals Influence of savory essential oil on the fatty acids composition in the brain and liver with age increasing of akr line mice

2011 ◽  
Vol 57 (6) ◽  
pp. 604-614 ◽  
Author(s):  
T.A. Misharina ◽  
E.B. Burlakova ◽  
L.D. Fatkullina ◽  
M.B. Terenina ◽  
N.I. Krikunova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Essential Oil ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Age Related

Age-related alterations of fatty acid composition in liver and brain of AKR mice was investigated. The effect of savory essential oil (Satureja hortensis L.), added with drinking water on fatty acid composition in these organs and the processes of lipid peroxidation in erythrocytes were estimated. It was found that during aging the percentage of saturated fatty acids and polyunsaturated fatty acids decreased while monounsaturated fatty acids increased. The development of leukemia was accompanied by the increase of saturated and polyunsaturated fatty acids percentage and a decrease of monounsaturated fatty acids amount. In the liver aging caused the increase in the percentage of saturated fatty acids, the decrease of monounsaturated fatty acids, while the amount of polyunsaturated fatty acids was not changed. Leukemia (after 8 month) was accompanied by the increase of percentage of monounsaturated fatty acids and the decrease in the amount of oleinic and docosohexaenic acids. The intake of savory essential oil was accompanied by intensification of polyunsaturated fatty acids synthesis in mice liver and reduction of lipid peroxidation products content.

scholarly journals Comparison of lipid classes and fatty acid compositions of farmed and wild pacific oysters, Crassostrea gigas, in Nha Trang, Vietnam

2020 ◽  
Vol 20 (4) ◽  
pp. 463-467
Author(s):  
Trinh Thi Thu Huong
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Crassostrea Gigas ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Lipid Classes ◽  
Pacific Oysters ◽  
Total Fatty Acids ◽  
First Time ◽  
Fatty Acid Compositions

Lipid classes and fatty acid compositions of the farmed (F-C. gigas) and wild (W-C. gigas) pacific oysters, Crassostrea gigas, in Nha Trang, Vietnam were investigated for the first time. The results indicated that the lipid classes and fatty acid components of these oysters were insignificantly different. The total lipid of both studied oysters included six lipid classes, namely phospholipid (PL), sterol (ST), free fatty acid (FFA), triacylglycerol (TG), monoalkyldiacylglycerol (MADG), and hydrocarbon-wax (HW) in which TG and PL were dominated with the values of 48.4%, 41.8% for TG and 19.0%, 20.3% for PL in F-C. gigas and W-C. gigas, respectively. The fatty acids (FAs) content of F-C. gigas and W-C. gigas was similar. The saturated fatty acids (SFAs) content was 48.2% in total fatty acids (TFAs) of the F-C. gigas and 44.7% in TFAs of W-C. gigas, in which 16:0 was dominated in SFAs of both oysters with the value of 24.2% in F-C. gigas and 22.0% in W-C. gigas. The contents of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) were 18.3%, 20.5% for MUFAs and 31.7%, 34.7% for PUFAs respectively in F-C. gigas and W-C. gigas. These MUFAs compositions contained 16:1n-7, 16:1n-5, 18:1n-9, 18:1n-7, 20:1n-11, 20:1n-9 and 20:1n-7, among them fatty acids 16:1n-7 (7.2% in F-C. gigas, 6.3% in W-C. gigas) and 18:1n-7 (6.6% in F-C. gigas, 7.4% in W-C. gigas) were the main MUFAs. PUFAs in these two oysters consisted of long-chain n-3 and n-6 fatty acids, in which 20:5n-3 (EPA) and 22:6n-3 (DHA) were dominated with the values of 12.7%, 13.9% for EPA in F-C. gigas and 6.5%, 6.0% for DHA in W-C. gigas.

Effects of Gamma Radiation on Microbiological Status, Fatty Acid Composition, and Color of Vacuum-Packaged Cold-Stored Fresh Pork Meat

2009 ◽  
Vol 72 (3) ◽  
pp. 556-563 ◽  
Author(s):  
VASSILIA J. SINANOGLOU ◽  
SPYROS KONTELES ◽  
ANTHIMIA BATRINOU ◽  
FOTIS MANTIS ◽  
KONSTANTINOS SFLOMOS
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Salmonella Enteritidis ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Contamination Level ◽  
Radiation Doses ◽  
Pork Meat ◽  
Total Fatty Acids

Pork meat samples were inoculated with high or low levels (106 or 103 CFU/g) of Salmonella Enteritidis, vacuum packaged, exposed to γ radiation (1.0, 2.5, and 4.7 kGy), and stored for 1 month at 4 ± 1°C. In highly contaminated samples, the target strain was completely eliminated only by the 4.7 kGy radiation dose, whereas in samples at the lower contamination level, 2.5 kGy was sufficient to eliminate Salmonella Enteritidis. The highest of the applied radiation doses reduced the aerobic microflora and extended the sample's refrigeration shelf life by at least 2 weeks. The fatty acid profile of pork meat was not significantly affected by any of the applied radiation doses. Irradiation increased the proportion of saturated fatty acids (SFA) and decreased the content of the polyunsaturated fatty acids (P &lt; 0.05). Irradiation also affected negatively the proportions of the nutritional indexes ω-6/ω-3, SFA/monounsaturated fatty acids, and SFA/polyunsaturated fatty acids. The proportion of the trans fatty acids C18:1ω-9 t9 and C18:2 t9,t12 in the total fatty acids was nearly doubled (90 and 86%, respectively) in the samples that had been exposed to 4.7 kGy. None of the applied radiation doses changed the lightness (L* value) of the meat, but redness (a*) and yellowness (b*) increased, particularly for the samples treated with 4.7 kGy.

scholarly journals Fatty Acid Compositions of Selected Polish Pork Hams and Sausages as Influenced by Their Traditionality

2018 ◽  
Vol 10 (11) ◽  
pp. 3885
Author(s):  
Michał Halagarda ◽  
Władysław Kędzior ◽  
Ewa Pyrzyńska ◽  
Wanda Kudełka
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Meat Products ◽  
Monounsaturated Fatty Acids ◽  
Fatty Acid Profiles ◽  
Traditional Foods ◽  
High Quality ◽  
Research Material ◽  
The World ◽  
Fatty Acid Compositions

Sausages and hams are perceived as important components of culinary heritage for many regions all over the world. Consumers believe that traditional foods are characterized by unique sensory properties and high quality. However, the fats found in all pork meat products are generally not associated with favorable dietary patterns. The aim of this study was to verify the possible differences regarding the composition of fatty acids between traditional Polish pork hams and wiejska sausages, and their conventional equivalents. For this purpose, the fat content and fatty acid profiles were determined. The research material consisted of 2 varieties of traditional hams and 5 varieties of sausages, as well as 4 varieties of both conventional hams and sausages. The results of this study demonstrated that traditional hams contained significantly higher percentage of C 20:3 (cis-11,14,17) acid than their conventional equivalents. Traditional sausages were characterized by lower shares of C 18:2 (cis-9,12) and Polyunsaturated Fatty Acids (PUFA), whereas higher content of C 18:1 (cis-9), C 18:3 (cis-9,12,15), C 20:0 and Monounsaturated Fatty Acids (MUFA). This resulted in significantly higher amounts of n-3 and lower of n-6 acids than in conventional sausages. All of the tested meat products were also characterized by an unfavorable n-6/n-3 ratio.

scholarly journals Fatty acid composition in trochophores of mussel Mytilus galloprovincialis grown under contamination with polychlorinated biphenyls

2020 ◽  
Vol 5 (2) ◽  
pp. 38-49
Author(s):  
L. L. Kapranova ◽  
L. V. Malakhova ◽  
M. V. Nekhoroshev ◽  
V. V. Lobko ◽  
V. I. Ryabushko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Environmental Pollution ◽  
Acid Composition ◽  
Mytilus Galloprovincialis ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Total Fraction

Status of Mytilus galloprovincialis populations in the natural habitat is known to directly depend on development of Black Sea mussel at all its stages, including initial stages of larval ontogenesis, which are very sensitive to environmental pollution. Organic pollutants adversely affect mussel larvae by inhibiting their growth and development. Patterns of mussel reproduction are well studied, which makes it possible to obtain larvae from artificially fertilized eggs of this mollusc species in controlled laboratory conditions. In this work, the fatty acid composition of M. galloprovincialis larvae at the trochophore stage on the 3rd day in the control experiment and under artificial contamination with polychlorinated biphenyls (PCBs) in different concentrations is studied for the first time. The fatty acid composition of total lipids in the biomass of larvae obtained on the 3rd day of the experiment was studied by means of gas chromatography – mass spectrometry. Totally, 14 fatty acids were identified in the samples; 59 % of them were saturated fatty acids, 24 % were monounsaturated fatty acids, and 17 % were polyunsaturated fatty acids. Statistical analysis was performed using Statistical Toolbox of MATLAB software (version 8.2). The totals of monounsaturated and polyunsaturated fatty acids significantly differed in lipids of M. galloprovincialis trochophores in the experiment with different PCB concentrations. The totals of saturated fatty acids did not significantly differ. The major saturated fatty acids in all mussel trochophores studied were palmitic (C16:0) and stearic (C18:0) acids. Their concentration did not significantly change under the exposure to PCBs. The main monounsaturated fatty acids were oleic (C18:1ω9), palmitoleic (C16:1ω7), and vaccenic (C18:1ω7) acids. The fraction of monounsaturated fatty acids was twice as low when exposed to the PCB concentrations 0.1 and 1.0 μg·L−1. However, when the PCB concentration was 10 μg·L−1, the total of these acids did not differ from the control. Among polyunsaturated fatty acids having biological essentiality, it was possible to identify arachidonic (C20:4ω6), eicosapentaenoic (C20:5ω3), and docosahexaenoic (C22:6ω3) acids. The total fraction of omega-3 and omega-6 acids in mussel larvae in the control did not exceed 12.8 %. With an increase of the PCB concentration in the growth medium 0.1 to 1.0 μg·L−1, the fraction of polyunsaturated fatty acids increased 2.5-fold. At the PCB concentration 10 μg·L−1 and in the sample with pure acetone added, the total fraction of polyunsaturated fatty acids was comparable with that in the control. The results of the study indicate that fatty acid response is the highest when the medium is exposed to the PCB concentrations ranging 0.1 to 1.0 μg·L−1. At the PCB concentrations equal to 10 μg·L−1 or higher, biochemical processes in larvae seem to slow down. The results of this study will contribute to a better understanding of biochemical rearrangements that allow molluscs at larval developmental stages to adapt to environmental pollution with organic xenobiotics.

scholarly journals Fatty acid profile of pork from a local and a commercial breed

2015 ◽  
Vol 58 (2) ◽  
pp. 379-385 ◽  
Author(s):  
A. Kasprzyk ◽  
M. Tyra ◽  
M. Babicz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Saturated Fatty Acid ◽  
Acid Composition ◽  
Monounsaturated Fatty Acids ◽  
Cholesterol Content ◽  
Drip Loss ◽  
Fatty Acid Compositions

Abstract. This study investigated the effects of breed on the fatty acid compositions of the Longissimus thoracis et lumborum (LTL) of gilts and barrows. Although only one muscle was analyzed, the results gave a good indication of the effect that breed and sex may have on the fatty acid compositions of the meat. Breed exhibited a significant effect on the fatty acid composition of pigs, whereas the effects of sex were found to be minor. Higher contents of intramuscular fat (IMF), C16 : 1, C18 : 1 and monounsaturated fatty acids (MUFAs); darker color of meat; and lower cholesterol content, drip loss, C18 : 0, C18 : 2, polyunsaturated fatty acids (PUFAs), n-6 and n-6 : n-3 ratios were found in the LTL muscle of Pulawska pigs compared with Polish Landrace pigs. Meat of Pulawska pigs is especially suitable for the production of good-quality, cured and smoked loin for longer storage. Fat content was higher in barrows than in gilts, and as a consequence the IMF from barrows had higher saturated fatty acid proportions and hypercholesterolemic acids (OFAs) as well as lower C18 : 1 than that from gilts.

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