scholarly journals Effect of Oral Intake of African Locust Bean on Fasting Blood Sugar and Lipid Profile of Albino Rats

2019 ◽  
pp. 1-9
Author(s):  
Augustine I. Airaodion ◽  
Edith O. Airaodion ◽  
Emmanuel O. Ogbuagu ◽  
Uloaku Ogbuagu ◽  
Etinosa U. Osemwowa
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Blood Sugar ◽  
Saline Solution ◽  
Fasting Blood Sugar ◽  
Control Group ◽  
Albino Rats ◽  
Locust Bean ◽  
African Locust Bean ◽  
Ibadan Nigeria

Background: African locust bean is a condiment believed to be for the people of low class. Its health importance has not been fully known especially to the so-called high class. Aim: This study is aimed at investigating the effect of African locust bean on fasting blood sugar and lipid profile of albino rats. Methods: African locust bean (ALB) was purchased from a local market at Orita-Challenge area of Ibadan, Nigeria. They were sun dried and milled into powder using an electronic blender. The powder was extracted with n-hexane (40–60°C) in a soxlet extractor for 18 hours. The defatted, dried marc was repacked and then extracted with methanol. The dried marc was extracted with methanol in the soxlet apparatus for 10 hours. The methanol solution was subsequently concentrated in a rotatory evaporator at 40°C. Ten adult male albino rats with body weight between 100 and 120 g were purchased from the Animal Holding Unit of the Department of Physiology, University of Ibadan, Nigeria. They were allowed 7 days acclimatization period and were divided randomly into 2 groups of five rats each. Animals in group 1 were administered normal saline solution while those in group 2 were administered extract of African locust bean. The animals were exposed to the African Locust Bean and saline solution at a dose of 3 ml per 100 g body weight 12 hourly via oral route of administration. After fourteen days of administration, the animals were fasted overnight and anaesthetized using diethyl ether. Blood samples were collected by cardiac puncture. Results: ALB was observed to significantly lower fasting blood sugar, total cholesterol and triglyceride but increased HDL-cholesterol significantly when compared to those of the control group at p<0.05. LDL-cholesterol was not significantly different when animals treated with ALB were compared with those of the control group. Conclusion: Increased blood sugar and hyperlipidemia has been implicated in diabetes. Cholesterolgenesis is a major onset of atherosclerosis and subsequent cardiovascular diseases. Therefore, ALB can serve as prophylaxis and remedies for several diseases caused by hyperglycemia and hyperlipidemia such as diabetes and coronary heart disease.

scholarly journals Effect of Methanolic Extract of Corchorus olitorius Leaves on Hypoglycemic and Hypolipidaemic Activities in Albino Rats

2019 ◽  
pp. 1-13 ◽  
Author(s):  
Augustine I. Airaodion ◽  
Joanne D. Akinmolayan ◽  
Emmanuel O. Ogbuagu ◽  
Edith O. Airaodion ◽  
Uloaku Ogbuagu ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Sugar ◽  
Saline Solution ◽  
Ldl Cholesterol ◽  
Methanolic Extract ◽  
Fasting Blood Sugar ◽  
Control Group ◽  
Albino Rats ◽  
Corchorus Olitorius ◽  
Soxhlet Apparatus

Aim: This study sought to investigate the hypoglycemic and hypolipidaemic effect of methanolic extract of Corchorus olitorius leaves in Albino rats. Methods: Fresh plants of C. olitorius were harvested from the Institute of Agricultural Research and Training, Ibadan. The leaves were dried and extracted using soxhlet apparatus and methanol as the solvent. The methanol was evaporated in a rotary evaporator at 35°C with a yield of 2.17 g which represents a percentage yield of 8.68%. Ten adult male albino rats with body weight between 100 and 120 g were used for this study. They were randomly divided into two groups of five rats each. Animals in group 1 were administered saline solution while those in group 2 were administered C. olitorius extract. The animals were exposed to the extract and saline solution at a dose of 3 mL per 100 g body weight 12 hourly via oral route of administration. After fourteen days of administration, the animals were fasted overnight and anaesthetized using diethyl ether. Blood samples were collected by cardiac puncture. Fasting blood sugar and lipid profile were determined using standard methods. Results: C. olitorius leaves was observed to significantly lower fasting blood sugar, total cholesterol, LDL-cholesterol and triglyceride but increased HDL-cholesterol and HDL/LDL-cholesterol ratio significantly when compared to those of the control group at p<0.05. Conclusion: The result of this study implies that C. olitorius leaves are of significant health importance as far as hyperglucosemia and hyperlipidaemia is concerned. It could also be exceedingly helpful in the control of obesity and hypertension. This pharmacological study is a useful tool for further drug development from the natural plant products.

scholarly journals The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues

2021 ◽  
Vol 9 (1) ◽  
pp. 22-25
Author(s):  
Asmaa Saad Mahmoud Shokhba ◽  
Mohamed A. Abdel-Rahman ◽  
Mohammed Alaa El-Deen A. Omran ◽  
Nahla Soliman El-Shenawy
Keyword(s):  
Body Weight ◽  
Vascular Permeability ◽  
Saline Solution ◽  
Public Awareness ◽  
Control Group ◽  
Albino Rats ◽  
Colorimetric Determination ◽  
Crude Venom ◽  
Treatment Groups ◽  
Renal Vascular

Background: Among venomous elapid snakes, cobras have the highest public awareness, as their venom represents a combination of proteins, peptides, and enzymes that have a range of biochemical and pharmacological roles and are also the main constitutes of biological activity and lethal toxicity. Objectives: The study aimed to evaluate the effect of the venom of Egyptian Spitting Cobra, Naja nubiae, on the vascular permeability based on the extravasation of the azo dye Evans blue (EB) into the tissues of the liver and kidneys of animals envenomed with low (¼ LD50; 0.32 mg/kg) and high (½ LD50; 0.65 mg/ kg) doses at three sampling times (30, 120, 360 min) post-injection of the venom. Methods: Fifty-four adult male Albino rats (8 weeks old and 180±2 0 g body weight) were divided into three main groups (n=6). In the control group, rats were subcutaneously (SC) injected with saline solution. Envenomed groups were SC injected, one group with 0.32 mg/kg and the other group with 0.65 mg/kg body weight of crude venom, respectively. Rats were I.V injected with EB dye 20 minutes before SC injection with saline solution as control animals and with Naja nubiae venom as treatment groups. Results: The results illustrated a high significant rate of EB extravasation to hepatic and renal tissues by the colorimetric determination of EB dye concentration. Conclusion: The venom of Naja nubiae can cause increased hepatic and renal vascular permeability which may explain the inflammatory effect induced by this venom.

scholarly journals A study on the status of serum High Sensitive C - Reactive Protein and Fasting Blood Sugar in Patients with Hyperlipidemia attending a Super Specialty Teaching Hospital in North Kerala

2020 ◽  
Vol 11 (4) ◽  
pp. 6908-6912
Author(s):  
Mohammed Muneersha T K ◽  
Saravanakumar R T ◽  
Revikumar K G ◽  
Fathima Habeeba T E ◽  
Jobin Louis ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Blood Sugar ◽  
Teaching Hospital ◽  
Blood Sugar Level ◽  
Fasting Blood Sugar ◽  
Control Group ◽  
Newly Diagnosed ◽  
Significant Difference ◽  
The Status ◽  
Hs Crp

This study was aimed to analyses the serum Hs-CRP level and fasting Blood Sugar level in patients with Hyperlipidemia in a Super Specialty Teaching Hospital in north Kerala. This was a hospital-based prospective observational study which was conducted to study the status of Hs-CRP and Fasting Blood sugar level on lipid profile status of newly diagnosed Hyperlipidemia patients which was compared with Normal Lipid Profile people. Healthy subjects were recruited as a control group and newly Diagnosed Hyperlipidemia patients were recruited as Test group. Study subjects of both gender in an age group of 30 to 70 were selected for the study. Serum Hs CRP, Fasting Blood sugar and serum lipid profile were estimated after overnight fasting condition. After the biochemical estimation, values were entered in an Excel sheet and level of test and control group was statistically analyzed by using Student's t-test using SPSS software. The values were expressed in Mean ± Standard Deviation (SD) and The status of serum Hs CRP, fasting Blood Sugar and Lipid Profile Parameters were shown a statistically significant difference (p<0.01) between Control and Test groups. The result of the study points out that the elevated level serum Hs-CRP and Fasting Blood Sugar can be used as predictive markers of Hyperlipidemia.

scholarly journals Effect of Methanolic Extract of Vernonia amygdalina Leaves on Glycemic and Lipidaemic Indexes of Wistar Rats

2019 ◽  
pp. 1-14
Author(s):  
Emmanuel O. Ogbuagu ◽  
Augustine I. Airaodion ◽  
Uloaku Ogbuagu ◽  
Edith O. Airaodion
Keyword(s):  
Body Weight ◽  
Total Cholesterol ◽  
Blood Sugar ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Methanolic Extract ◽  
Fasting Blood Sugar ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Soxhlet Apparatus

Aim: This study sought to investigate the effect of methanolic extract of Vernonia amygdalina leaves on glycemic and lipidaemic indexes of Wistar rats. Methods: Fresh plants of V. amygdalina were harvested from the Institute of Agricultural Research and Training, Ibadan. The leaves were dried and extracted using soxhlet apparatus and methanol was used as the solvent. The solvent was recovered in a rotary evaporator at 35°C with a yield of 2.24 g which represents a percentage yield of 8.96%. Ten adult male Wistar rats with body weight between 100 and 120 g were used for this study. They were randomly divided into two groups of five rats each. Animals in group 1 were administered normal saline while those in group 2 were administered V. amygdalina extract. The animals were exposed to the extract and saline solution at a dose of 3 mL per 100 g body weight 12 hourly via oral route of administration. After fourteen days of administration, the animals were fasted overnight and anaesthetized using diethyl ether. Blood samples were collected by cardiac puncture. Fasting blood sugar and lipid profile were determined using standard methods. Results: V. amygdalina  leaves was observed to significantly lower fasting blood sugar, total cholesterol, triglyceride and VLDL-cholesterol but had no significant effect on the concentrations of HDL-cholesterol, LDL-cholesterol and HDL/LDL-cholesterol ratio when compared to those of the control group at p<0.05. Conclusion: The lowering effect of V. amygdalina leaves on fasting blood sugar indicated that it has the propensity to be antidiabetic. Its effect on total cholesterol and triglyceride showed that V. amygdalina leaves could be useful in the control of obesity and hypertension.

scholarly journals Study on Synergistic Effect of Bromocriptine and Sitagliptin in Streptozotocin-induced Diabetic Rats

2021 ◽  
Author(s):  
Govindaraj Nileshraj ◽  
Chandrasekaran Swithraa ◽  
Murugesan Sakthibalan ◽  
Maruti Shripati Sawadkar
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Lipid Profile ◽  
Blood Sugar ◽  
Wistar Rats ◽  
Low Dose ◽  
Diabetic Rats ◽  
Fasting Blood Sugar ◽  
Liver Parameters ◽  
Dose Combination

Introduction: Diabetes mellitus is a potentially morbid condition with high prevalence worldwide. Restoration of euglycemia in type 2 diabetes mellitus is difficult to achieve due to its multifactorial pathogenesis and frequently requires multiple anti-diabetic medications with different mechanism of action. This study is based on the possible synergistic effect of Sitagliptin and Bromocriptine in reducing blood sugar in diabetic animal models. Aim: To evaluate the effect of Sitagliptin and Bromocriptinemonotherapy and their combination in three different doses on blood sugar, Lipid profile, Liver and renal parameters in diabetic albino Wistar rats. Materials and Methods: An experimental animal study was conducted on 48 adult male albino wistar rats for a period of one month (August 2014). Streptozotocin induced (40 mg/kg IP) diabetic rats were divided into six groups of eight animals each weighing 150-200 gms. Group I- Vehicle (distilled water), Group II- Sitagliptin (100 mg/kg), Group III- Bromocriptine (10 mg/kg), Group IV- Sitagliptin (75 mg/kg) + Bromocriptine (7.5 mg/kg), Group V- Sitagliptin (100 mg/kg) + Bromocriptine (10 mg/kg), Group VI- Sitagliptin (125 mg/kg) + Bromocriptine (12.5 mg/kg). All drugs were given once daily (p.o) for four weeks. Body weight, fasting blood sugar, fasting lipid profile, liver parameters and renal parameters were estimated before and after administration of the drugs. Results were analyzed with SPSS software version 16.0 using one-way ANOVA followed by post-hoc Dunnett t test. P<0.05 was considered statistically significant. Results: The low dose combination therapy [Sitagliptin (75 mg/kg) + Bromocriptine (7.5 mg/kg)] showed a significant decrease in body weight (p<0.05), fasting blood sugar (p<0.001) compared with the vehicle group. It also showed a favorable lipid profile, renal and liver parameters compared with the vehicle, monotherapy and other combination regimens. Conclusion: The low dose combination therapy of Sitagliptin and Bromocriptine showed a better control over fasting blood sugar along with a beneficial effect on lipid profile, liver parameters and renal parameters than compared to other groups.

Effect of leaf extracts of Carica papaya (Paw paw) and Pakia biglobosa (African locust bean) on some biochemical parameters of albino rats

2021 ◽  
Vol 1 (2) ◽  
pp. 014-021
Author(s):  
Michael Okey Enemali ◽  
Kingsley Ikechukwu Ubaoji ◽  
Chinenye Enoch Oguazu ◽  
Gambo Sunday Haruna ◽  
Kingsley Kelechi Asogwa
Keyword(s):  
Biochemical Parameters ◽  
Carica Papaya ◽  
Control Group ◽  
Albino Rats ◽  
Serum Concentrations ◽  
Leaf Extracts ◽  
Orthodox Medicine ◽  
Laboratory Methods ◽  
African Locust Bean ◽  
Made In

In spite of the advances made in orthodox medicine, there has been an increasing interest in herbal medicine. The leaves of Carica papaya and Pakia biglobosa have been reported to contain lots of beneficial medicinal compounds, hence their use in the traditional prevention, management and treatment of ailments/diseases. In this study, the effect of varied concentrations of the ethanol leaf extract of the plants on some biochemical parameters of albino rats was assessed. The phytochemical compositions of the leaves were determined using established standard laboratory methods. Fifty four male Albino rats weighing between 150g-200g were randomly distributed into nine groups of six animals each. A daily single dose of 500mg/kg, 1000mg/kg, 1500mg/kg and 2000mg/kg body weight of either of the extracts was respectively, administered to the eight test groups for fourteen days. The control group was given only feed and water. Biochemical parameters such as the serum activities of Aspartate Aminotransferase, Alanine Aminotranferase and Alkaline phosphatase as well as the serum concentrations of Bilirubin, Albumin, HCO3-, Urea, Creatinine and Na+ were assessed. Result of the analyses showed that the administration of the extracts did not significantly raise the serum activity of ALT. The decreases in serum concentrations of Urea and increase in the concentration of HCO3- were directly proportional to the concentration of the extracts. It can be deduced from this study, that 500mg, 1000mg 1500mg and 2000mg/kg bw of either C. papaya or P. biglobosa did not elicit any marked hepatotoxicological or renotoxicological effect on the experimental animals

scholarly journals Inhibition ofα-Glucosidase by Thiosulfinate as a Target for Glucose Modulation in Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Abdulrahman L. Al-Malki
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Hydrolytic Enzyme ◽  
Positive Control ◽  
Diabetic Rats ◽  
Postprandial Hyperglycemia ◽  
Postprandial Blood Glucose ◽  
Control Group ◽  
Albino Rats ◽  
Predisposing Factor

Postprandial hyperglycemia is a predisposing factor for vascular dysfunction and organ damage.α-glucosidase is a hydrolytic enzyme that increases the glucose absorption rate and subsequently elevates blood glucose levels. Garlic (Allium sativumL.) is a rich source of several phytonutrients, including thiosulfinate (THIO). The aim of this study was to evaluate the ability of THIO, a potent inhibitor of intestinalα-glucosidase, to reduce postprandial blood glucose. Male albino rats were randomly assigned to five different groups (n=10/group). Group 1 served as the control group. Groups 2–5 were injected intraperitoneally with a single dose of streptozotocin (STZ) to induce diabetes. Group 2 comprised untreated diabetic rats. Groups 3 and 4 contained diabetic rats that were given THIO orally (20 mg/kg body weight/day and 40 mg/kg body weight/day, resp.). Group 5 was the positive control having diabetic rats treated orally with acarbose (10 mg/kg body weight/day; positive control). Diabetic rats treated with THIO displayed a significant blood glucose reduction (p<0.001and < 0.01 by analysis of variance, resp.) and a significant elevation in insulin compared with that of untreated rats. THIO is an effective noncompetitive intestinalα-glucosidase inhibitor that promotes hypoglycemic action (p<0.001) in STZ-injected rats. THIO is a promising agent for the management of postprandial hyperglycemia.

scholarly journals Effects of quail (Coturnix japonica) egg diet on both the blood sugar and the lipid profile of alloxan induced diabetic albino rats

2018 ◽  
Vol 29 (19) ◽  
Author(s):  
Umera AE ◽  
Ejezie FE ◽  
Ibegbu MD ◽  
Ikekpeazu JE ◽  
Onyekwelu KC ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Blood Sugar ◽  
Coturnix Japonica ◽  
Albino Rats
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