scholarly journals Microbial Assessment and Proximate Composition of Pepper (Capsicum annum) and Tomato (Solanum lycopersicum) Displayed for Sales

2021 ◽  
pp. 100-112
Author(s):  
Omorodion Nnenna ◽  
Alabo Briggs
Keyword(s):  
Solanum Lycopersicum ◽  
Heterotrophic Bacteria ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Vegetable Crops ◽  
Bacterial Isolates ◽  
Candida Sp ◽  
Capsicum Annum ◽  
Fusarium Sp ◽  
Lower Count

Tomato (Solanum lycopersicum) and pepper (Capsicum annuum) are among the world’s most important vegetable crops. A total of 20 samples made up of fresh tomatoes, spoilt tomatoes, fresh pepper and spoilt pepper were analysed in this study. Standard microbiological practices were carried out on the samples. Total Heterotrophic Bacterial (THB) Count for Spoilt tomatoes ranged from 1.98x107cfu/g to 2.39x107cfu/g. Fresh tomatoes samples had a THB counts ranging from 1.43x106cfu/g to 2.14x106cfu/g. Spoilt pepper total heterotrophic bacteria counts ranged from 1.46x107cfu/g to 2.01x107cfu/g. Fresh pepper had a THB count of 1.22x106cfu/g to 1.66x106cfu/g. Spoilt tomatoes samples had a higher fungal count that ranged from 3.5x105cfu/g to 5.25x105cfu/g while the fresh tomatoes samples had lower fungal counts ranging from 1.95x104cfu/g to 3.45x104cfu/g. The spoilt pepper had fungal counts ranging from 1.45x105cfu/g to 2.65x105cfu/g which was higher than that of fresh pepper with a low count of 1.6x104cfu/g to 2.75x104.cfu/g. The bacterial isolates identified during this study are Staphylococcus sp., Escherichia coli, Bacillus sp., klebsiella sp., Pseudomonas sp., Shigella sp., Protues sp., Enterobacter sp., Citrobacter sp., Lactobacillus sp. Micrococcus sp. Listeria sp. Streptococcus sp. and Serratia sp. Spoilt tomatoes had the highest coliform count 1.02x105cfu/g to 9.0x105cfu/g while fresh tomatoes had a lower count of 4.65x105cfu/g to 6.75x105cfu/g. Spoilt pepper recorded coliform counts ranging from 3.8x105cfu/g to 9.4x105cfu/g. Most of the fungi isolated from both the tomatoes and pepper samples were molds and yeast which include Aspergillus niger, Aspergillus flavus, Candida sp., Saccharomyces sp., Penicillium sp. Mucor sp. and Fusarium sp. Adequate cooking is recommended before consumption.

scholarly journals Microbial Flora of Smoke-Dried Catfish (Clarias Gariepinus) Processed and Sold in Some Abattoirs in Bayelsa and Rivers States

2021 ◽  
Vol 4 (4) ◽  
pp. 123-133
Author(s):  
Bosede A.A. ◽  
Omokaro O.
Keyword(s):  
Heterotrophic Bacteria ◽  
Clarias Gariepinus ◽  
Total Coliform ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Total Hydrocarbon ◽  
Penicillium Sp ◽  
Fusarium Sp ◽  
Total Fungi ◽  
Rhizopus Sp

The microflora of smoked catfish (Clarias gariepinus) sold in some abattoirs in Bayelsa and Rivers States were investigated. Smoked catfish samples were purchased within abattoirs and aseptically transported in ice-packed coolers to the laboratory. The total heterotrophic bacteria, total coliform, total hydrocarbon utilizing bacteria, total fungi and hydrocarbon utilizing fungal counts and identification of isolates from samples were analyzed using standard microbiological methods. Mean values of counts obtained showed that total heterotrophic bacteria ranged from 5.4 × 106CFU/g to 4.0 × 105CFU/g, total hydrocarbon utilizing bacteria ranged from 1.5 × 104 to 1.0 × 103CFU/g, total coliform ranged from 1.7 × 106CFU/g to 0, total fungi ranged from 4.3 × 104 CFU/g to 1.2 × 104CFU/g and total hydrocarbon utilizing fungi ranged from 3.5 × 103CFU/g to 1.1 × 103CFU/g. Kruskal Walis H test showed no significant differences (P≤0.05) in the total heterotrophic bacterial load nor in the total fungal load in the locations. Bacteria identified are Bacillus sp, Enterobacter sp, Escherichia coli, Micrococcus sp, Pseudomonas sp, Salmonella sp., and Shigella species. Bacillus sp, Pseudomonas sp and Micrococcus sp., were isolated in all the samples. Salmonella sp and Shigella sp occurred in Igbogene and Swale samples. Bacillus sp recorded the highest occurrence (34%) while Enterobacter sp. and Micrococcus sp recorded the least (7%). Bacteria with hydrocarbon utilizing potentials with percentage occurrence were Bacillus sp (70%) and Pseudomonas sp (30%). Fungi isolated were Aspergillus niger, A. flavus, Aspergillus fumigatus, Penicillium sp, Fusarium sp., and Rhizopus sp. Aspergillus species recorded the highest frequency (36.0%) while Rhizopus sp recorded the least (9.83%). Aspergillus niger, Fusarium sp and Penicillium sp were isolated from all the samples. Penicillium sp recorded hydrocarbon utilizing the potential and the highest percentage of occurrence (35.89%). The presence of a high microbial load of pathogenic bacteria and known mycotoxin producing fungi in the smoke-dried catfish are of great public health significance.

scholarly journals Enhanced Biodegradation of Degreaser Using Pseudomonas and Bacillus Species in Fresh Water Ecosystem

2019 ◽  
pp. 1-10
Author(s):  
Renner Renner Nrior ◽  
Indutimi Mathias Otuogha
Keyword(s):  
Fresh Water ◽  
Heterotrophic Bacteria ◽  
Bacillus Species ◽  
Freshwater Ecosystem ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Microbiological Methods ◽  
Pseudomonas Species ◽  
Set Up ◽  
Hydrocarbon Utilizing Bacteria

Aim: The aim of this study is to enhance the biodegradation of degreasers used in upstream sectors of Nigeria Petroleum Industry using bio-augmenting organisms such as: Pseudomonas and Bacillus species in freshwater Ecosystem. Study Design: This study employs experimental designs, Randomized Block Design treatment set up, statistical analysis of data and interpretation. Place and Duration of Study: Freshwater sample for this research was collected from Asarama Andoni, in Rivers State, Nigeria. The study lasted for six months. Methodology: The experimental set-up was carried in 500 ml conical flask with two species of bacteria, two types of degreaser and fresh water sample giving a total of 8 set-up including controls. The Pseudomonas and Bacillus species used in this study were isolated from the freshwater ecosystem and identified using standard microbiological methods. The bioremediation potential of the respective test organisms were monitored at ambient temperature 28±0.2°C for 28 days at a constant interval of 7 days using the following Physiochemical parameter; Total dissolved Solid, Hydrogen concentrations ions and Total Hydrocarbon Content. While the following Microbiological parameters; Total heterotrophic Bacteria, Total Heterotrophic Fungi, Hydrocarbon Utilizing Bacteria, and Hydrocarbon Utilizing Fungi were monitored. Results: The percentage of degradability of the respective set-ups ranged from Control (Rigwash) (3.29%) < Pseudomonas sp. + Rigwash (27.56%) < Pseudomonas + Bacillus + Rigwash< (31.57%), Bacillus sp.+ Rigwash (37.57%) Control 2 (Aquabreak) (9.45%) < Pseudomonas sp.+ Aquabreak (26.77%) < Pseudomonas + Bacillus + Aquabreak (31.32%)< Bacillus sp.+ Aquabreak (32.46%). Overall evaluation revealed that Bacillus sp. had a higher biodegradation potential on both degreaser (Rigwash and Aquabreak) in freshwater than Pseudomonas sp. Five species of bacteria: Escherichia coli, Micrococcus, Citrobacter, Bacillus, and Pseudomonas species and four fungal species: Penicillium, Mucor, Aspergillus and Rhizopus species were isolated and identified as hydrocarbon utilizing bacteria and fungi organisms respectively. Conclusion: The results revealed that Bacillus species have more degradability potential than Pseudomonas species for both Aquabreak and Rigwash. These results also indicated the low biodegradation potential of Rigwash in fresh Ecosystem.

KARAKTERISASI Bacillus spp. DAN Fusarium sp. DARI TANAMAN LADA (Piper nigrum L.) DI DESA JAGA

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Florianus Flori ◽  
Mukarlina Mukarlina ◽  
Rahmawati Rahmawati
Keyword(s):  
Piper Nigrum ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Genus Bacillus ◽  
Isolation And Identification ◽  
West Kalimantan ◽  
Bacillus Spp ◽  
Fusarium Sp ◽  
Farm Crop ◽  
Fusarium Fungi

Pepper (Piper nigrum L.) is one of the farm crop which become commodities in West Kalimantan. This research aimed to determine the morphological characteristic of Bacillus bacteria from rhizosphere of pepper and Fusarium fungi with symptoms of yellow disease. Sampling was done in pepper farm crops, Sungai Jaga Village, Bengkayang Regency. Isolation and identification were implemented at Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak. The result of isolation and identification showed 4 bacterial isolates belong to genus Bacillus, namely Bacillus sp. BRF1, Bacillus sp. BRF2, Bacillus sp. BRF3 and Bacillus sp. BRF4 and one isolate of Fusarium sp. JDF from pepper with symptom of yellow desease.

scholarly journals Diversity of Biofilm-Forming Bacteria in Chinnamuttom Harbor of Southeast India

2021 ◽  
Vol 14 (4) ◽  
pp. 1793-1799
Author(s):  
P. Nithya
Keyword(s):  
Surface Water ◽  
Bacterial Species ◽  
Ship Hull ◽  
Rrna Gene ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Four Seasons ◽  
The Mean ◽  
Marine Environmental

Biofilms are species rich, partially due to highly effective powers of diffusion of the microorganisms and have wide tolerance to marine environmental conditions. Characteristically, the first organisms to respond to and convalesce from stress. The present study aims to isolate and identify the biofilm forming bacterial species, collected from surface water and substratum of the ship hull for four seasons at Chinnamuttom fishing harbor, Southeast coast of India, during June 2015 to May 2016. Among the mean concentration of bacterial isolates of both water and substratum of the station, maximum in monsoon and minimum in summer seasons. Totally 16 isolates were obtained, based on the adherence property, 8 isolates from surface water and 8 isolates from substratum of the ship hull. The samples were plated on Zobell marine agar medium for bacterial isolates of study area. The isolates of Bacillus sp., Flavobacterium sp., Pseudomonas sp., Aeromonas sp., Micrococcus sp., Vibrio sp., Salmonella sp., Staphylococcus sp., Shegella sp., Klebsiella sp., Corynebacterium sp., Enterobacter sp., Chromohalobacter sp., Bacillus sp., Escherichia coli and Bacilus sp. were reported in all the seasons at study area. The major diverse bacterial isolates were further characterized through morphological and molecular identification. Based on 16S rRNA gene sequences. Biofilm bacterial isolates were confirmed as Bacillus sp., and Pseudomonas sp.

scholarly journals Microbiological and Physicochemical Characteristics of Abattoir Wastewaters in Bayelsa and Rivers State

2021 ◽  
pp. 32-45
Author(s):  
Ariyo, Adenike Bosede ◽  
Obire, Omokaro
Keyword(s):  
Escherichia Coli ◽  
Physicochemical Parameters ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Penicillium Sp ◽  
Untreated Wastewater ◽  
Significant Difference ◽  
Fusarium Sp ◽  
The Mean ◽  
Rivers State

Wastewater samples from abattoirs in Bayelsa and Rivers State were evaluated for their microbiological and physicochemical properties. The microbiological parameters were analyzed using standard microbiological techniques while the Physicochemical parameters were analyzed based on the APHA standard method. The mean range of the total heterotrophic bacterial, bacterial, total coliform, total Vibrio, total hydrocarbon utilizing, total heterotrophic fungal and hydrocarbon utilizing fungal counts of the wastewater were: (1.9×107to 5.2×107, 2.1×105 to 4.0 × 105, 1.0×103 to 3.1 × 103, 1.0×105 to 2.2×105, 1.8×105 to c4.1×105 and 1.1×103 to 3.2 ×103)CFU/ml respectively. Kruskal-Wallis H test showed that there was no significant difference (P≥0.05) in the counts for the bacterial and fungal counts. Bacillus sp (17.32%), Escherichia coli (16.33%), Streptococcus sp (15.34%), Staphylococcus sp (14.35%), Pseudomonas sp (11.38%), Shigella sp (10.39%) Alcaligenes sp (9.9%), Vibrio sp (6.93%) and Salmonella sp (4.93%), were the bacteria isolated. The percentage of occurrence of hydrocarbon utilizing bacteria isolates were: Bacillus sp (34.28%), Pseudomonas sp (26.71%), Alcaligenes sp (20%). Escherichia coli (14.28%), Staphylococcus sp (5.71%). Among the fungi isolated Aspergillus niger, Fusarium sp, Geotrichum sp were found in 18.75% each while Penicillium sp recorded 23% occurrence, Aspergillus fumigatus and Candida sp were found in 6.25% occurrence each but Mucor sp exhibited 8.33% of occurrence. The percentages of occurrence of hydrocarbon utilizing fungi were Aspergillus sp (22.22%), Fusarium sp (16.66%), Penicillium sp (33.33%), Geotrichum sp (16.66%), Mucor sp (5.55%) and Candida (5.55%). The mean of physicochemical parameters was: pH: 6.7-9.1, EC: 156-4981, Turbidity: 11.1-149NTU, TSS: 62-239, TDS: 104-3320, NO3: 20.97-30.97, SO4: 14.5-54.6, PO4: 1.78-20.21, salinity: 41.25-2800, DO: 4.29-41.18, COD:3600-5476.0 and BOD-:1600.0-7761.4. The high microbial load and physicochemical parameters in these wastewaters is alarming and could pose serious human health and environmental challenges if the disposal of untreated wastewater continues unabated.

scholarly journals Caracterización in vitro de rizobacterias y su antagonismo con hongos causantes del damping off en chile

2018 ◽  
Vol 9 (3) ◽  
pp. 525-537
Author(s):  
Eyra Judith Hernández Hernández ◽  
Ismael Hernández Ríos ◽  
Juan José Almaraz Suarez ◽  
Aline López López ◽  
Margarita Torres Aquino ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Pseudomonas Chlororaphis ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Damping Off ◽  
Fusarium Spp ◽  
Capsicum Annuum L ◽  
Fusarium Sp

La producción de plántulas de chile (Capsicum annuum L.) en almácigos es afectada por la enfermedad conocida como “damping off”. Su prevención se hace regularmente mediante fungicidas, cuyos efectos sobre los hongos causantes de esta enfermedad no son siempre efectivos, pero representan problemas de tipo ambiental. Ante esta situación, el control biológico adquiere mayor relevancia. En el presente trabajo se aislaron tres cepas de hongos fitopatógenos del género Fusarium spp. (FP, FN y FA; por los colores púrpura, naranja y amarillo, respectivamente). En cuanto a cepas bacterianas, se seleccionaron 18 cepas de rizobacterias (B), las cuales fueron confrontados in vitro con las tres cepas de Fusarium y una de Rhizotocnia (R). La cepa B23 fue la más eficiente en la inhibición del crecimiento de los fitopatógenos estudiados, al reducir el crecimiento de FP, FN y FA (50, 44 y 47%, respectivamente) y de R (56%); la producción de AIA varió entre 1.24 y 11.32 μg mL-1, la mayor solubilización de fosfato inorgánico (104 μg mL-1) se obtuvo con B8. Al evaluar el efecto de B7, B9, B15 y B23 sobre la supervivencia de plántulas de chile inoculadas con Fusarium sp. y Rhizotocnia sp., se observó que las cepas B7 y B23 son las que tuvieron mayor efecto sobre la supervivencia de las plántulas durante los primeros 34 días, con valores de entre 80 a 100%, mientras que las plántulas control fue de entre 30 y 50%. Por otra parte, las cepas B8 y B9 se identificaron molecularmente como Pseudomonas chlororaphis; B7 y B14 como Pseudomonas sp.; B15 como Pseudomonas protegens y B23 como Bacillus sp. Los resultados muestran la potencialidad del uso de bacterias como promotoras del crecimiento y agentes de biocontrol de hongos causantes del damping off en plántulas de chile tipo “guajillo”.

scholarly journals Effect of Co-Inoculation of Bacillus sp. Strain with Bacterial Endophytes on Plant Growth and Colonization in Tomato Plant (Solanum lycopersicum)

2021 ◽  
Vol 12 (2) ◽  
pp. 480-490
Author(s):  
Ahsanul Salehin ◽  
Ramesh Raj Puri ◽  
Md Hafizur Rahman Hafiz ◽  
Kazuhito Itoh
Keyword(s):  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Growth Promotion ◽  
Tomato Plant ◽  
Growth Promotion ◽  
Growth Parameters ◽  
The Other ◽  
Time Interval ◽  
Bacillus Sp ◽  
F 33

Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was compared with three rhizospheric and endophytic Bacillus sp. strains to evaluate the colonization potential of the Bacillus sp. strains with a different origin. Surface-sterilized seeds of tomato (Solanum lycopersicum L. cv. Chika) were sown in the sterilized vermiculite, and four Bacillus sp. strains were each inoculated onto the seed zone. After cultivation in a phytotron, plant growth parameters and populations of the inoculants in the root, shoot, and rhizosphere were determined. In addition, effects of co-inoculation and time interval inoculation of Bacillus sp. F-33 with the other endophytes were examined. All Bacillus sp. strains promoted plant growth except for Bacillus sp. RF-37, and populations of the rhizospheric and endophytic Bacillus sp. strains were 1.4–2.8 orders higher in the tomato plant than that of Bacillus sp. OYK. The plant growth promotion by Bacillus sp. F-33 was reduced by co-inoculation with the other endophytic strains: Klebsiella sp. Sal 1, Enterobacter sp. Sal 3, and Herbaspirillum sp. Sal 6., though the population of Bacillus sp. F-33 maintained or slightly decreased. When Klebsiella sp. Sal 1 was inoculated after Bacillus sp. F-33, the plant growth-promoting effects by Bacillus sp. F-33 were reduced without a reduction of its population, while when Bacillus sp. F-33 was inoculated after Klebsiella sp. Sal 1, the effects were increased in spite of the reduction of its population. Klebsiella sp. Sal 1 colonized dominantly under both conditions. The higher population of rhizospheric and endophytic Bacillus sp. in the plant suggests the importance of the origin of the strains for their colonization. The plant growth promotion and colonization potentials were independently affected by the co-existing microorganisms.

Enhanced Toxicity of Bacillus thuringiensis Subspecies kurstaki and aizawai to Black Cutworm Larvae (Lepidoptera: Noctuidae) With Bacillus sp. NFD2 and Pseudomonas sp. FNFD1

2011 ◽  
Vol 104 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Tamer A. Mashtoly ◽  
Assem Abolmaaty ◽  
Mohamed El-Said El-Zemaity ◽  
Mohamed I. Hussien ◽  
Steven R. Alm
Keyword(s):  
Bacillus Thuringiensis ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Black Cutworm ◽  
Lepidoptera Noctuidae

scholarly journals Sorção de cobre e manganês por bactérias rizosféricas do trigo

2001 ◽  
Vol 31 (6) ◽  
pp. 947-951 ◽  
Author(s):  
Marcio Voss ◽  
Robert Wayne Stefen Phillip Thomas
Keyword(s):  
Pseudomonas Sp ◽  
Bacillus Sp

A bioacumulação de metais por microrganismos se deve principalmente a fenômenos de superfície, ocorrendo adsorção, de forma estequiométrica, com os radicais aniônicos dos envoltórios celulares, seguido ou não de precipitação dos metais. Para estudar condicionantes da sorção de metais por bactérias vivas, quantificou-se o Cu2+ e Mn2+ retirados por um Bacillus sp. e uma Pseudomonas sp., isolados da rizosfera de trigo, de uma solução de cloreto dos metais, determinando-se a quantidade de metal restante no sobrenadante, após centrifugação. Usou-se delineamento experimental inteiramente casualizado, com três repetições. Ensaiaram-se efeito dos teores de Cu2+ e Mn2+, do pH e do tempo de crescimento bacteriano. O Bacillus sorveu mais Cu2+ e Mn2+ do que Pseudomonas. em todas as concentrações desses metais. A sorção de Cu2+ por ambas as bactérias apresentou maiores incrementos do que Mn2+ com aumento dos teores desses metais na solução. A alteração do pH 5,0 para 3,0 diminuiu a sorção dos dois metais. Com o tempo de cultivo de 90 horas a Pseudomonas apresentou maior sorção de cobre e de manganês do que no tempo de 16 horas. Os resultados obtidos assemelham-se aos fenômenos de troca de cátions em colóides.

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