Determination of Pharmacological and Therapeutic Potential of Cissus quadrangularis L.

Synthesis, characterization and Cytotoxicity Evaluation of New Heterocyclic Compounds with Oxazole Ring Containing 4-(Phenylsulfonyl)phenyl Moiety

Revista de Chimie ◽

10.37358/10.37358/rc.70.19.11.7649 ◽

2019 ◽

Vol 70 (11) ◽

pp. 3793-3801

Keyword(s):

Therapeutic Potential ◽

Hplc Method ◽

Oxazole Ring ◽

Acyl Chlorides ◽

Rp Hplc ◽

Toxicological Profile ◽

Ft Ir ◽

Cytotoxicity Assessment ◽

New Compounds

The paper presents the synthesis, characterization and cytotoxicity assessment of five organic compounds containing 4-(phenylsulfonyl)phenyl fragment in the molecule, namely of three acyclic precursors derived from phenylalanine (from N-acyl-a-amino acids, N-acyl-a-amino acyl chlorides and N-acyl-a-amino ketones class) and of the cyclization products: a 1,3-oxazol-5(4H)-one and, respectively, a 1,3-oxazole substituted in position 5 with the p-tolyl group. The synthesized compounds were characterized by spectral methods (UV-Vis, FT-IR, 1H-NMR, 13C-NMR, and MS) and elemental analysis, which confirmed their structures. For the determination of the purity of the new compounds, the RP-HPLC method was used. In view of the therapeutic potential of the newly synthesized compounds, we evaluated their toxicological profile using the Daphnia magna bioassay. Keywords: N-acyl-a-amino acid, 1,3-oxazol-5(4H)-one, N-acyl-a-amino ketone, 1,3-oxazole, cytotoxic effect

Download Full-text

Determination of the therapeutic potential of human umbilical cord blood

Journal of Pharmacy & Bioresources ◽

10.4314/jpb.v13i2.10 ◽

2016 ◽

Vol 13 (2) ◽

pp. 148

Author(s):

Faith I. Akinnibosun ◽

Phebean O. Ozolua

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Therapeutic Potential ◽

Human Umbilical Cord Blood ◽

Human Umbilical Cord

Download Full-text

Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502012000400003 ◽

2012 ◽

Vol 48 (4) ◽

pp. 601-607 ◽

Cited By ~ 4

Author(s):

Naila Abdul Sattar ◽

Fatma Hussain ◽

Tahira Iqbal ◽

Munir Ahmad Sheikh

Keyword(s):

Therapeutic Potential ◽

Zingiber Officinale ◽

Postprandial Glucose ◽

Protein Glycation ◽

Zingiber Officinale Roscoe ◽

In Vitro Effects ◽

Dose Dependent ◽

Antidiabetic Effects

Aqueous extracts of Zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 g/L. The antidiabetic effects were found to be dose-dependent. Antidiabetic potential of Zingiber officinale was mainly through inhibition of the glucose diffusion and to a limited extent by reducing the glycation. However, further studies are needed to determine in vitro effects of therapeutic potential by restraining postprandial glucose absorptions and plasma protein glycations in diabetic subjects.

Download Full-text

Visual Imagery Reactivity: Relationships to Rorschach Responses, Diagnostic Classification and Therapeutic Potential

Psychological Reports ◽

10.2466/pr0.1979.45.3.1003 ◽

1979 ◽

Vol 45 (3) ◽

pp. 1003-1010 ◽

Cited By ~ 6

Author(s):

Alexander Yanovski ◽

Max L. Fogel

Keyword(s):

Individual Differences ◽

Visual Imagery ◽

Therapeutic Potential ◽

Diagnostic Classification ◽

Diagnostic Categories ◽

Imagery Techniques

Visual imagery techniques were applied to a randomly selected group of 30 patients in therapy to determine individual differences in response to such techniques and to examine several diagnostic and therapeutic correlates of these differences. Analysis showed that, when patients were classified as Imagery Reactors or Nonreactors, there were corresponding differences associated with this classification with respect to (1) general diagnostic categories, (2) degree and quality of Rorschach responses including a conflict material score, and (3) success in insight-oriented therapy utilizing imagery. It was concluded that determination of a patient's level of imagery reactivity provides a valuable ancillary tool in selected diagnostic and therapeutic situations.

Download Full-text

Antiadipogenic Effects ofAster glehniExtract: In Vivo and In Vitro Effects

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/859624 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Heon-Myung Lee ◽

Gabsik Yang ◽

Tae-Gue Ahn ◽

Myung-Dong Kim ◽

Agung Nugroho ◽

...

Keyword(s):

Therapeutic Potential ◽

Control Diet ◽

Epididymal Adipose Tissue ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Master Regulators ◽

In Vitro Effects

Aster glehni(AG) is a Korean traditional herb that grows in Ulleungdo Island, Republic of Korea. None of the several reports on AG include a determination of the effect of AG on adipogenesis. The primary aim of this study was to determine whether AG attenuates adipogenesis in mouse 3T3-L1 cells and epididymal fat tissue. AG blocked the differentiation of 3T3-L1 preadipocytes in a concentration-dependent manner and suppressed the expression of adipogenesis-related genes such asPPARγ,C/EBPα, andSREBP1c, the master regulators of adipogenesis. Male C57BL/6J mice were divided randomly and equally into 4 diet groups: control diet (CON), high-fat diet (HFD), HFD with 1% AG extract added (AG1), and HFD with 5% AG extract added (AG5). The experimental animals were fed HFD and the 2 combinations for 10 weeks. Mice fed HFD with AG gained less body weight and visceral fat-pad weight than did the mice fed HFD alone. Moreover, AG inhibited the expression of important adipogenic genes such asPPARγ,C/EBPα,SREBP1c,LXR, and leptin in the epididymal adipose tissue of the mice treated with AG1 and AG5. These findings indicate antiadipogenic and antiobesity effects of AG and suggest its therapeutic potential in obesity and obesity-related diseases.

Download Full-text

The ketogenic diet: a co-therapy in the treatment of mood disorders and obesity - a case report

Current Problems of Psychiatry ◽

10.2478/cpp-2021-0002 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Agata Pieklik ◽

Martyna Pawlaczyk ◽

Joanna Rog ◽

Hanna Karakuła-Juchnowicz

Keyword(s):

Body Weight ◽

Ketogenic Diet ◽

Therapeutic Potential ◽

Primary Source ◽

Nutritional Intervention ◽

Elevated Concentration ◽

Body Weight Reduction ◽

Reduced Body ◽

The Central Nervous System

Abstract Introduction: There has been a growing interest in the ketogenic diet (KD) due to its suggested therapeutic potential to support numerous chronic diseases. KD is characterized by high amounts of fats and a reduced amount of carbohydrates and protein intake. During following the nutrition protocol, ketones are synthesised, which are the primary source of energy. The elevated concentration of ketones in blood serum inhibits hunger, what leads to reduced body weight. Some authors suggest KD has antidepressant potential and could stabilise mood by affecting neurotransmitters homeostasis in the central nervous system. Material and methods: The aim of the study was to assess the effect of KD on body weight reduction and improvement of mood in the patients with mood disorder diagnosis. To interpret the results of nutritional intervention, the laboratory parameters and structuralised scales and questionnaires were used. Results: After following 4-week therapy, the reduction of body weight, correction of some laboratory measurements and reduction in mood symptoms were noticed. Conclusions: The ketogenic diet affects the anthropometric measurements. However, a variety of simultaneous therapeutic approaches makes impossible determination of the effect on depressive symptoms.

Download Full-text

The Role of Scleraxis in Fate Determination of Mesenchymal Stem Cells for Tenocyte Differentiation

Scientific Reports ◽

10.1038/srep13149 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 37

Author(s):

Yonghui Li ◽

Melissa Ramcharan ◽

Zuping Zhou ◽

Daniel J. Leong ◽

Takintope Akinbiyi ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Therapeutic Potential ◽

Maximal Response ◽

Multipotent Stem Cells ◽

Fate Determination ◽

Tenogenic Differentiation ◽

Morphogenetic Protein ◽

Embryonic Tendon

Abstract Mesenchymal stem cells (MSCs) are pluripotent cells that primarily differentiate into osteocytes, chondrocytes and adipocytes. Recent studies indicate that MSCs can also be induced to generate tenocyte-like cells; moreover, MSCs have been suggested to have great therapeutic potential for tendon pathologies. Yet the precise molecular cascades governing tenogenic differentiation of MSCs remain unclear. We demonstrate scleraxis, a transcription factor critically involved in embryonic tendon development and formation, plays a pivotal role in the fate determination of MSC towards tenocyte differentiation. Using murine C3H10T1/2 pluripotent stem cells as a model system, we show scleraxis is extensively expressed in the early phase of bone morphogenetic protein (BMP)-12-triggered tenocytic differentiation. Once induced, scleraxis directly transactivates tendon lineage-related genes such as tenomodulin and suppresses osteogenic, chondrogenic and adipogenic capabilities, thus committing C3H10T1/2 cells to differentiate into the specific tenocyte-like lineage, while eliminating plasticity for other lineages. We also reveal that mechanical loading-mediated tenocytic differentiation follows a similar pathway and that BMP-12 and cyclic uniaxial strain act in an additive fashion to augment the maximal response by activating signal transducer Smad8. These results provide critical insights into the determination of multipotent stem cells to the tenocyte lineage induced by both chemical and physical signals.

Download Full-text

Employing Endogenous NSCs to Promote Recovery of Spinal Cord Injury

Stem Cells International ◽

10.1155/2019/1958631 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Sumei Liu ◽

Zhiguo Chen

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Therapeutic Potential ◽

Central Canal ◽

Injured Tissue ◽

Cord Injury ◽

Heterogeneous Nature ◽

Adult Hippocampus

Endogenous neural stem cells (NSCs) exist in the central canal of mammalian spinal cords. Under normal conditions, these NSCs remain quiescent and express FoxJ1. After spinal cord injury (SCI), the endogenous NSCs of a heterogeneous nature are activated and proliferate and migrate towards the lesion site and mainly differentiate into astrocytes to repair the injured tissue. In vitro, spinal cord NSCs are multipotent and can differentiate into neurons, astrocytes, and oligodendrocytes. The altered microenvironments after SCI play key roles on the fate determination of activated NSCs, especially on the neuronal specification potential. Studies show that the activated spinal cord NSCs can generate interneurons when transplanted into the adult hippocampus. In addition, the spinal cord NSCs exhibit low immunogenicity in a transplantation context, thus implicating a promising therapeutic potential on SCI recovery. Here, we summarize the characteristics of spinal cord NSCs, especially their properties after injury. With a better understanding of endogenous NSCs under normal and SCI conditions, we may be able to employ endogenous NSCs for SCI repair in the future.

Download Full-text