Antimicrobial Evaluation and GC-MS Analysis of Strobilanthes crispus Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

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GC–MS analysis of ethanolic leaf extract of Capparis divaricata Lam.

Current Botany ◽

10.25081/cb.2020.v11.6017 ◽

2020 ◽

pp. 34-35

Author(s):

M. Hemalatha ◽

Saivenkatesh Korlam ◽

N. Yasodamma

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Medicinal Plants ◽

Andhra Pradesh ◽

Leaf Extract ◽

Ethanolic Extract ◽

Ms Analysis ◽

Pharmacological Studies ◽

Based Medicine ◽

Ethanolic Leaf Extract

Planet earth is rich in plant-based medicine. Unfortunately, most of the medicinal plants are not in lime light for their optimal utilisation by the mankind mostly, because of unknown medicinally important phytochemicals present in them. Capparis divaricata Lam. is one of the medicinally important plants widely distributed in Arthagiri Hills of Chittoor Dist., Andhra Pradesh. The plant is ethnobotanically used for different ailments especially cancer. In the present study, Gas Chromatography and Mass Spectrometry (GC-MS) analysis of leaf ethanolic extract of Capparis divaricate is reported. Octasilaxone, Hexadecamethyl; Acetoxymethyl – Trimethyl: Octamethyl were identified as the major compounds. Further, these compounds may act as anti-cancerous agents by efficient pharmacological studies.

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EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.26470 ◽

2019 ◽

Vol 12 (1) ◽

pp. 520

Author(s):

Sharmila S ◽

Ramya E K

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Cytotoxic Activity ◽

Cell Line ◽

Leaf Extract ◽

Gas Chromatography Mass Spectrometry ◽

Chromatography Mass Spectrometry ◽

Ms Analysis ◽

Wide Range ◽

Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

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GC-MS Analysis of Bioactive Compounds in Ethanolic Leaf Extract of Hellenia speciosa (J.Koenig) S.R. Dutta

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-021-03742-2 ◽

2021 ◽

Author(s):

Ramya R

Keyword(s):

Bioactive Compounds ◽

Leaf Extract ◽

Ms Analysis ◽

Ethanolic Leaf Extract

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Phytochemical and chromatographic characterization of Mimosa diplotricha Wright ex. Sauvalle ethanolic leaf extract and assessment of antioxidant potential and anti-proliferative effect on colorectal cancer (HCT-116) cell lines

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i3.2656 ◽

2020 ◽

Vol 11 (3) ◽

pp. 4378-4387

Author(s):

Sri Rashmy Madathil ◽

Poornima Kannappan ◽

Devaki Kanakasabapathy ◽

Sincy Varghese ◽

Perumalsamy Muneeswari

Keyword(s):

Cell Lines ◽

Mtt Assay ◽

Leaf Extract ◽

Antioxidant Potential ◽

Antioxidant Power ◽

Ms Analysis ◽

Chromatographic Profile ◽

Hct 116 ◽

Ethanolic Leaf Extract

Present study aims to evaluate phytochemical and chromatographic profile, along with antioxidant and anti proliferative properties of Mimosa diplotricha ethanolic leaf extract. Qualitative screening of phytoconstituents by consecutive solvent extraction in increased polarity basis and standardization of potential extract based on phytochemical elution profile was done. Selected secondary metabolites like phenols, flavanoids, tannins and alkaloids were quantified in ethanolic extract. Chromatographic profile was determined by HPTLC and GC-MS analysis. In vitro antioxidant potential was assessed by DPPH, superoxide, nitric oxide, hydrogen peroxide and hydroxyl radical scavenging assay. Ferric-reducing antioxidant power assay (FRAP) and reducing potential of the respective extract were also determined. Anticancer potential was confirmed by cytotoxic screening in colorectal (HCT-116) cancer cell lines by MTT assay. Qualitative phytochemical analysis and chromatographic profile reveal a phytoconstituent rich profile for the ethanolic leaf extract. The amount of, phenols (56 ± 0.57 mg/g), flavanoids (27 ± 0.76 mg/g), tannins (33 ± 0.15 mg/g) were quantified as equivalent of gallic acid, quercetin and tannic acid standards respectively and alkaloids (2.51 ± 0.47 mg/g of extracted plant material) were expressed based on respective analysis. Results also reveal convincing antioxidant potential for respective extract. In vitro cytotoxicity confirmed by MTT assay represents an IC50 value of 97.82 µg/ml. From the above results it can be concluded that M.diplotricha has got pharmacologically significant phytoconstituents and therapeutic active ingredients as evident in HPTLC and GC-MS analysis. This is further supported by considerable antioxidant and anti proliferative properties observed in respective assays.

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GC-MS Analysis of Bioactive Compounds on Ethanolic Leaf Extract of Phyllodium pulchellum L. Desv.

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/ijpapr.v9i1.8050 ◽

2017 ◽

Vol 9 (1) ◽

Author(s):

Velmurugan G. ◽

Anand S. P.

Keyword(s):

Gas Chromatography ◽

Mass Spectrum ◽

Bioactive Compounds ◽

Leaf Extract ◽

Biological Activities ◽

Ms Analysis ◽

Phytochemical Compounds ◽

Minor Compounds ◽

Therapeutic Activities ◽

Ethanolic Leaf Extract

The present investigation was carried out to characterize the bioactive compounds present in leaf extract of Phyllodium pulchellum using Gas Chromatography-Mass Spectrum (GC-MS). The results of the GC-MS analysis provide different peaks determining the presence of 10 phytochemical compounds with different therapeutic activities. The major phytocompounds 9-Octadecenamide, (Z) (18.89), 9-Cyclohexylnonadecane (15.93), 2,2,2-trifluoroethyl 2- methyltetrahydro-5-oxo-3-furancarboxylate (10.47) and minor compounds were also present. From the results, it could be concluded that P.pulchellum contains various bioactive compounds have various biological activities. Therefore, it is recommended as a plant of phytopharmaceutical importance.

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GC-MS analysis of bioactive compounds in ethanolic leaf extract of Hellenia speciosa (J.Koenig) S.R.Dutta

10.21203/rs.3.rs-743090/v1 ◽

2021 ◽

Author(s):

Ramya Raveendran

Keyword(s):

Methyl Ester ◽

Bioactive Compounds ◽

Leaf Extract ◽

Acid Methyl Ester ◽

Acid Methyl ◽

Ms Analysis ◽

The Family ◽

Intestinal Worms ◽

Phytochemical Components ◽

Ethanolic Leaf Extract

Abstract Hellenia speciosa (J.Koenig) S.R.Dutta, is a plant species belonging to the family Costaceae. It is widely distributed in China, India, Malaysia, Indonesia, tropical and subtropical Asia. In Ayurveda, the rhizome of the plant has been extensively used to treat fever, rash, asthma, bronchitis and, intestinal worms. The objective of the present study was to investigate the phytochemical constituents of the leaf of Hellenia speciosa using Gas Chromatography and Mass Spectroscopy analysis (GC-MS). The GC-MS analysis revealed the presence of 17 phytochemical components in the ethanolic leaf extract of Hellenia speciosa. The prevailing bioactive compounds present in Hellenia speciosa were Thymol (RT-10.019;3.59%), Caryophyllene (RT-11.854:0.62%), Caryophyllene oxide (RT-13.919;1.34%), Artumerone (RT-14.795;1.35%), Hexadecanoic acid methyl ester (RT-17.536; 2.77%), 9,12-Octadecanoic acid methyl ester (RT-19.163;1.35%), Squalene (RT- 24.980;1.19%), Piperine (RT-25.745;3.11%), Beta Tocopherol (RT-26.681;2.88%) Vitamin E (RT- 27.290;2.64%), Progesterone (RT-29.608;3.18%), Caparratriene (RT-29.861;9.72%), and Testosterone (RT-30.73;5.81%). The compounds were identified by comparing their retention time and peak area with that of the literature and by interpretation of mass spectra. The results and findings of the present study suggest that the plant can be used as a valuable source in the field of herbal drug discovery. The presence of bioactive compounds justifies the use of plant leaves for treating various diseases with fewer side effects and recommended the plant of pharmaceutical importance. However, further studies are needed to undertake its bioactivity and toxicity profile.

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