scholarly journals Effect of Rhustox on Micropropagation of Scoparia dulcis L. through Leaf Explants Culture

2019 ◽  
pp. 1-14
Author(s):  
Sayema Zeya ◽  
Maheshwar Prasad Trivedi
Keyword(s):  
Leaf Explants ◽  
Botanical Garden ◽  
Optimum Temperature ◽  
Free Medium ◽  
Culture Initiation ◽  
Plant Materials ◽  
Experimental Part ◽  
Scoparia Dulcis ◽  
Science College

Aim: To investigate the effect of Rhus toxicodendron (30CH) along with different compositions of phytohormones (Auxin and Cytokinin) on the basis of  growth and multiplication of explants under optimum temperature under in-vitro conditions. Study Design: To establish and design the standard protocol for the in-vitro propagation through leaf explant of Scoparia dulcis  under stress of phytohormones and homeopathic medicine Rhus toxicodendron (30CH). Place and Duration of Study: The plant materials were  procured from the Herbal Botanical Garden Patna Science College, Department of Botany, Patna University, Patna, Bihar. The experimental part was carried out in Plant Tissue Culture Laboratory, between December 2017 to August 2018  in Department of  Botany P.U. Patna. Methodlogy: The sterilized leaf explants were inoculated into MS media fortified with different phytohormones  (Auxin and Cytokinin) and Rhus tox(30CH) under aseptic environmental conditions for the growth and development of callus, embryoids etc. Result: The explants in MS medium supplemented with auxins phytohormones and Rhus tox(30CH) exhibited that IAA (0.10 to 2.0 mg/l) and BAP (0.10 to 2.5 mg/l)  induces green and compact calli. Whereas at 0.30mg/l of IAA and 0.50 mg/l BAP induced brown friable calli. 2,4-D (1.5 mg/l) and Kinetin (1.5-6.5mg/l) concentrations induced brown and friable calli. Rhus tox(30CH) (100 µl/100 ml) enhances  proliferation with 2,4-D and Kinetin (1.5/1.5 mg/l.). Conclusion:  After 42 days of culture initiation and establishment the callus was 520.0±1.12 mg in the mixture of 2,4-D and Kinetin (1.5 mg/l) in Rhus tox free medium. Whereas weight of callus were found to be 1092±0.74 mg after 42 days in the same medium of 2, 4-D and Kinetin (1.5/5.5 mg/l) supplemented with Rhus tox (100 µl/100 ml). Hence, the investigation proponded that the Rhus tox (CH30) has increased the rate of callus development and plantlet regeneration.

scholarly journals Honokiol and Magnolol Production by in vitro Micropropagated Plants of Magnolia dealbata, an Endangered Endemic Mexican Species

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Fabiola Domínguez ◽  
Marco Chávez ◽  
María Luisa Garduño-Ramírez ◽  
Víctor M. Chávez-Avila ◽  
Martín Mata ◽  
...  
Keyword(s):  
Growth Medium ◽  
Leaf Explants ◽  
Wild Plants ◽  
Root Induction ◽  
Ms Medium ◽  
Plant Materials ◽  
Detection Techniques ◽  
Magnolia Dealbata ◽  
Selection Of

An efficient protocol for the in vitro propagation of Magnolia dealbata Zucc., an important medicinal plant that is the source of the anxiolytic and anticancer compounds honokiol and magnolol, was established. This plant is wild-crafted, and conservationists have expressed concerns with regard to the sustainability of production. In the present work, two factors were found to be of importance for the regeneration of M. dealbata and the production of honokiol and magnolol. These factors were the type of explants and the combination and concentration of plant-growth regulators. Green, compact, nodular organogenic callus was obtained from leaf explants in a medium fortified with Murashige and Skoog salts and supplemented with 1.5 mg/L 2,4-dicholorophenoxyacetic acid and 1.5 mg/L kinetin. Shoot multiplication from callus cultures was achieved in the Murashige and Skoog (MS) medium with 1.5 mg/L thidiazuron (TDZ). Phenol secretion was controlled by the addition of 250 mg/L of activated charcoal. For rooting, shoots were transferred to MS medium supplemented with several auxins. After root induction, the plants were hardened in earthen pots containing sand, soil, and vermiculite. The contents of honokiol (HK) and magnolol (MG) were determined in different plant materials by high-performance liquid chromatography-diode-array detection techniques. This analysis revealed that the honokiol and magnolol content in aerial and underground parts of micropropagated M. dealbata were higher than that observed in wild plants (both 6 months old). Our results suggest that conservation of M. dealbata is possible by means of in vitro multiplication of leaf-derived callus. The usefulness of M. dealbata regeneration and production of HK and MG may be attributed to the proper selection of explant sourcing and identification of the correct growth medium to support adequate growth. This careful selection of explants and growth medium leads to a very useful source of plant material for pharmacological and phytomedicinal screening applications and, above all, would safeguard this plant species from the threat of extinction.

scholarly journals Histological studies of in vitro regeneration induced in leaf explants of Nymphoides cristatum (Roxb) O.Kuntze – An aquatic medicinal plant

1970 ◽  
Vol 3 ◽  
Author(s):  
Mallapa Hanumanthu Niranjan ◽  
Mysore Shankar Sudarshana
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Free Medium ◽  
Histological Studies ◽  
Leaf Histology ◽  
Benzyl Amino Purine

The objective of this work was to study the histological events related to the regeneration process of a medicinal plant, Nymphoides cristatum (Roxb). Leaf explants were cultured on MS medium supplemented with 0.5 mgl-1 of 6-benzyl amino purine (BAP). About 90% of explants gave rise to shoots after 15 days of incubation. The histological studies showed that the regeneration originated directly from parenchymatous cells and direct organogenesis after 20 days of culture could be observed. Buds and roots were found completely differentiated after 40 days of culture and number of shoots per explants was 30. Micorshoots were rooted in hormone - free medium and the plants obtained grew in artificial pond under green house conditions. Key words: Leaf, Histology, in vitro regeneration, Nymphoides cristatum.  DOI: 10.3126/ijls.v3i0.2370

scholarly journals In vitro Propagation and Callus Formation of Bacopa monnieri (L.) Penn.

1970 ◽  
Vol 20 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Patni Showkat ◽  
Yaseer Zaidi ◽  
Suhail Asghar ◽  
Shamsuddin Jamaluddin
Keyword(s):  
Callus Formation ◽  
Cluster Formation ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Bacopa Monnieri ◽  
Culture Initiation ◽  
Root Shoot Ratio ◽  
Mother Plants

An important medicinal herb, namely Bacopa monnieri (L.) Penn. showed best results on MS with 1 mg/l IAA and 1 mg/l IBA for culture initiation, multiple shoot proliferation, bud breakage, shoot length, shoot numbers and cluster formation. For rooting the best result was obtained on MS solidified with 7 g/l of agar along with sugar 20 gm/l for root, shoot ratio and number of roots. The best hardening was achieved in a mixture of soil : soilrite (1 : 1) where the survival rate was 94%. Further, for callus formation the best result was obtained in the leaf explants on MS supplemented with 0.5 mg/l 2, 4-D. HPLC and HPTLC profile of regenerated shoots revealed a phytochemicals profile similar to that of the market samples and mother plants. The presence of bacoside was detected through HPTLC and HPLC.    Key words:  Bacopa monnieri, Micropropagation, Bacoside   D.O.I. 10.3329/ptcb.v20i2.6890   Plant Tissue Cult. & Biotech. 20(2): 119-125, 2010 (December)

scholarly journals Stimulation of shoot regeneration through leaf thin cell layer culture of Passiflora edulis Sims.

2020 ◽  
Vol 16 (4) ◽  
pp. 669-677
Author(s):  
Tran Hieu ◽  
Do Thi Thuy Tam ◽  
Nguyen Thi Nhat Linh ◽  
Hoang Thanh Tung ◽  
Huynh Gia Bao ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Passiflora Edulis ◽  
Lighting Condition ◽  
Regeneration Rate ◽  
Micro Propagation ◽  
Passiflora Edulis Sims

Passiflora edulis Sims. belonged to the genus Passiflora, is one of the important economic crops of the world as well as Vietnam. Nowadays, the commercial P. edulis is mainly propagated by seeds, cuttings and grafting; however, these methods still have some limitations such as genetic degradation and heterogeneity and the spread of pathogenic viruses. Micro-propagation has been used for clonal breeding and disease-free plant breeding, as well as providing a source of materials for Passiflora breeding. In this study, leaf explants of P. edulis Sims. (2.0-month-old) excised from the in vitro culture of ex vitro axillary buds cut longitudinally and transversally into thin cell layers (lTCL and tTCL) were used as plant materials to evaluate the shoot regeneration. In addition, the effects of explant age and lighting condition on shoot regeneration were also investigated. After 8 weeks of culture, the results showed that shoot regeneration rate (100%) and shoot multiplication coefficient (13.33) of the in vitro leaf-tTCL-4 were higher than those of other treatments and control. The shoot regeneration rate of P. edulis Sims. also varied with the change of explant age. The highest shoot regeneration rate (100%) was obtained from leaf explants of 1.5-month-old shoots after 8 weeks of culture. Moreover, the light (fluorescent lamps with photoperiod of 16 hours/day and lighting intensity of 40 - 45 μmol.m-2.s-1) improved not only morphogenesis rate, but also shoot regeneration rate (100%) of leaf explants after 8 weeks of culture. This study provided a novel method for rapid micro-propagation of P. edulis Sims.

IN VITRO UPTAKE OF TRITIATED OESTRADIOL BY THE ANTERIOR HYPOTHALAMUS AND HYPOPHYSIS OF THE RAT

1970 ◽  
Vol 64 (4) ◽  
pp. 687-695 ◽  
Author(s):  
Junzo Kato
Keyword(s):  
Incubation Medium ◽  
Posterior Hypothalamus ◽  
Anterior Hypothalamus ◽  
Ovariectomized Rats ◽  
Free Medium ◽  
Cortex Cerebri ◽  
Anterior Hypophysis ◽  
In Vitro Uptake

ABSTRACT The anterior, middle, and posterior hypothalamus, the cortex cerebri, the anterior hypophysis as well as the diaphragm of adult ovariectomized rats were incubated in vitro with tritiated 17β-oestradiol. The uptake of tritiated oestradiol was differentially distributed intracerebrally with higher accumulation in the anterior hypothalamus and the hypophysis. Lowering the temperature of the incubation medium caused a reduction in the uptake of radioactivity by the anterior hypothalamus as compared to that found in other brain tissues. Tritiated oestradiol taken up in vitro by the anterior hypothalamus and the hypophysis tended to be retained after further incubation in a steroid-free medium. The addition of non-radioactive 17β-oestradiol to the medium inhibited the uptake of tritiated oestradiol by these tissues. Moreover, pretreatment with non-radioactive 17β-oestradiol in vivo prevented the preferential accumulation of tritiated oestradiol in vitro in the anterior hypothalamus and the hypophysis. These results indicate that oestradiol is preferentially taken up in vitro by the anterior hypothalamus and the hypophysis of the rat.

Evaluation of Antibacterial and Antidiabetic Potential of Silver Nanoparticles using Eugenia Uniflora L. Seed Extract

2020 ◽  
Vol 13 (6) ◽  
pp. 5217-5225
Author(s):  
Guru Kumar Dugganaboyana ◽  
Chethankumar Mukunda ◽  
Suresh Darshini Inakanally
Keyword(s):  
Biomedical Applications ◽  
Pathogenic Bacteria ◽  
Seed Extract ◽  
Drug Formulation ◽  
Visible Spectroscopy ◽  
Plant Materials ◽  
X Ray ◽  
Eugenia Uniflora ◽  
Uv Visible

In recent years, green nanotechnology-based approaches using plant materials have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the seed extract of the Eugenia uniflora L. (E.uniflora). Characterization was done using UV-Visible spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-Visible spectroscopy (at 466 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in seed of E.uniflora extract accountable for the reduction of Ag+ ion and the stabilization of AgNPs was investigated. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AgNPs were evaluated for their antibacterial and along with their antidiabetic potential. The results showed that AgNPs are extremely effective with potent antidiabetic potential at a very low concentration. It also exhibited potential antibacterial activity against the three tested human pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for in vitro evaluation of their antibacterial and antidiabetic effects with potent biomedical applications.

FACTORS AFFECTING THE REGENERATION OF PEPPER (CAPSICUM ANNUUM L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1120G-1120
Author(s):  
J. L. Jacobs ◽  
C. T. Stephens
Keyword(s):  
Growth Hormone ◽  
Silver Nitrate ◽  
Callus Formation ◽  
Leaf Explants ◽  
Regeneration System ◽  
Factors Affecting ◽  
Nitrate Concentrations ◽  
Leaf Differentiation ◽  
Whole Plants

Several growth hormone combinations and silver nitrate concentrations were examined for their effect on regeneration of different pepper genotypes. Primary leaf explants from in vitro seedlings were cultured on a revised Murashige and Skoog medium supplemented with auxin, cytokinin and 1.6% glucose. Combinations of different concentrations of indole-3-acetic acid (IAA), 0-5 mg/l, and 6-benzylaminopurine (BAP), 0-5 mg/l, were tested to determine the most effective medium for shoot primordium formation. Experiments with IAA and BAP did not result in a specific growth hormone combination appropriate for regeneration of all genotypes tested. Of the silver nitrate concentrations tested, 10 mg/l resulted in the best shoot and leaf differentiation and reduced callus formation. Differences in organogenic response of individual genotypes were evaluated on a single regeneration medium. Whole plants were regenerated from 11 of 63 genotypes examined. Based on these experiments, a reproducible regeneration system for pepper was developed with a total of 500 plants regenerated to date.

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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