scholarly journals Antidiabetic Activity of Aqueous and Ethanolic Extracts of Stem Barks from Trichilia emetica (Meliaceae) in Alloxan-Induced Diabeticalbinos Rats

2020 ◽  
pp. 17-24
Author(s):  
Djoupo Agnon Prisca ◽  
Dere Kwadjo Anicet Luc ◽  
Manhan Kahissié ◽  
Yapi Houphouet Félix ◽  
Tiahou Gnomblesson Georges
Keyword(s):  
Blood Pressure ◽  
Blood Glucose ◽  
Biochemical Parameters ◽  
Diabetes Management ◽  
Ethanolic Extract ◽  
Good Alternative ◽  
Antidiabetic Activity ◽  
Insulin Levels ◽  
Ethanolic Extracts ◽  
Antidiabetic Effects

Aims: This study was designed to evaluate the antidiabetic effects of aqueous and ethanolic extracts of Trichilia emetica (TE) stem bark in albinos rats. Methodology: 24 rats of comparable weight were divided into 2 lots, including a control lot of 3 rats and a test lot of 21 rats. Diabetes was induced by injecting Alloxan® intraperitoneally (125 mg/kg bw) for 7 days. After the onset of diabetes, the animals were divided into several groups and given the extracts of Trichilia emetica and Diastabol® orally for 6 days according to the following protocol: Normal Control (NC) received normal saline, group diabetic control (DC) it consists of untreated diabetic, group DD10 and DD20 was comprised of diabetic animals treated  with Diastabol® at a doses of 10  and 20 mg/kg bw respectively , group DTEE100 and DTEE200 it consists of diabetic animals treated with ethanolic extract at a doses of 100 and 200 mg/kg bw respectively, group DTEA100 and DDTEA 200 was comprised of diabetic animals treated with aqueous extract at a same dose (100 and 200 mg/kg bw). At the end of experimentation, some blood was collected for the determination of some biochemical parameters such as insulin, blood glucose, AST, ALT, urea and creatinine. Systolic blood pressure (SBP), diastolic blood pressure (DBP), cardiac frequency (CF) were also recorded.  Results: The results showed a significant (P<0.05) decrease in insulin levels in diabetic rats compared with NC. In addition, diabetes caused a significant increase (P<0.05) in blood glucose, urea, creatinine levels, transaminase activity, and in blood pressure numbers (DBP, SBP and CF) still in comparison with NC. However, the treatment of sick animals with the extracts and the Diastabol at the doses listed above significantly (P <0.05) increased insulin levels and reduced the sames biochemical parameters levels as well as the blood pressure numbers compared with DC. Conclusion: The data obtained showed that this part of the plant would have antidiabetic effects with ethanolic extract at the dose of 200 mg/kg bw had the highest pronounced effect and could be used as a good alternative for diabetes management, thus justifying its use in traditional medicine.

scholarly journals THE EFFECT OF ETHANOLIC EXTRACT OF DAYAK ONION (Eleutherine palmifolia (L) MERR) TUBER ON BLOOD GLUCOSE AND INSULIN LEVEL OF STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RAT

2019 ◽  
Vol 7 (4) ◽  
pp. 38-42
Author(s):  
Hasni Yaturramadhan ◽  
Aminah Dalimunthe ◽  
Tri Widyawati
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Insulin Level ◽  
Wistar Rat ◽  
Antidiabetic Activity ◽  
Plasma Insulin Concentration ◽  
Insulin Levels ◽  
Glucose Levels ◽  
Reduce Blood Glucose ◽  
Decrease Blood Glucose

Objectives: The current investigation was conducted to examine the ability of ethanolic extract from onion dayak (Eleutherine palmifolia) (L) (Merr) tuber to reduce blood glucose and increase insulin production in streptozotocin-induced diabetic wistar rat. Materials and Methods: Extraction was accomplished using maceration method with 96% ethanol. Antidiabetic activity was evaluated using observation of blood glucose levels utilize glucometer (Easy Touch). Analysis of insulin plasma concentration was determined utilize the Insulin Elisa Kit (Chem Cruz). Results: The administration of dayak onion extract as a form of therapy in diabetes, although it has not been able to increase insulin levels significantly, but has shown a tendency to increase insulin levels at higher doses of 500 mg/bw. Conclusion: Ethanolic extract from dayak onion tuber exhibited activity of decrease blood glucose level and increase plasma insulin concentration in streptozotocin-induced diabetic wistar rat.  

Ameliorative potential of Crinum asiaticum Linn leaf extracts on Elevated blood glucose in Alloxan-induced Diabetic Rats

2021 ◽  
pp. 6359-6363
Author(s):  
Abhinav Mishra ◽  
Vikram Singh ◽  
Raj K Prasad ◽  
Mohd Habeeb Ahmad
Keyword(s):  
Blood Glucose ◽  
Dose Level ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Oral Toxicity ◽  
Leaf Extracts ◽  
Glucose Lowering ◽  
Ethanolic Extracts ◽  
Crinum Asiaticum

In the present investigation the glucose lowering potential of the leaf extracts of Crinum asiaticum were prepared using cold maceration technique in solvents of varying polarity. The extracts exhibited the presence of flavonoids, alkaloids, phenolics and tannins. The oral toxicity of the aqueous and ethanolic extracts was determined and these two extracts were used of evaluating the antidiabetic activity. Oral glucose tolerance test was performed and diabetes was induced using alloxan (150 mg/kg) in rats. Ethanolic and aqueous extracts at two dose levels (200 mg/kg and 400mg/kg) were used for evaluating glucose lowering capability. Both the aqueous and the ethanolic extracts were found to significantly reduce glucose levels with the aqueous extract at dose level 200 mg/kg being the most effective (50% reduction) whereas the ethanolic extract was able to reduce the blood glucose by around 35% at the same dose level.

scholarly journals EVALUATION OF ANTIHYPERGLYCAEMIC EFFECT OF BUTEA MONOSPERMA LEAF EXTRACT ON ADRENALIN INDUCED AND HIGH GLUCOSE FEED ANIMAL MODEL

2017 ◽  
Vol 10 (12) ◽  
pp. 301
Author(s):  
Biswaranjan Ray ◽  
Santosh Kumar Mahapatra ◽  
Pradipta Kumar Behera ◽  
Ashok K Panigrahi
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
High Glucose ◽  
Ethanolic Extract ◽  
Biologically Active ◽  
Antidiabetic Activity ◽  
Blood Levels ◽  
Active Components ◽  
Butea Monosperma

Objective: As per the ethnopharmacological information has Butea monosperma been used to treat diabetes mellitus by the tribal people of tropical and subtropical areas. However, there is no much more scientific report available about the antidiabetic property of the leaves of the plant. Hence, the study was undertaken to evaluate the antidiabetic effect of ethanolic extract of B. monosperma on blood levels of adrenaline-induced and glucose feed diabetic rabbits.Methods: The three different doses of the extracts (100, 200, and 400 mg/kg) were administered orally to an experimental animal. The animals were induced diabetics by adrenaline and high glucose diet. Blood glucose level was measured accordingly. For antidiabetic activity, photocolorimeter was used to monitoring the blood glucose level with crest kit box (GOP-POD method).Results: The extracts showed considerable dose-dependent activity. However, the dose 400 mg/kg showed considerable lower of blood glucose level. p<0.01 indicates the significance result. 8 hrs reading 182.5±3.83 for 400 mg does is most effective for reducing blood sugar.Conclusion: The study indicates that the ethanolic extract of B. monosperma leaves possesses antidiabetic properties which suggest the presence of biologically active components.

scholarly journals Comparative evaluation of anti-diabetic action and pancreatic histopathology of rats treated with two alkaloidal plant extracts

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1841-1846
Author(s):  
Bonagiri Sreedevi ◽  
Vijaya Kuchana ◽  
Shobharani S
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Standard Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

This study aimed to understand Strychnosnuxvomica and Holarrhena pubescens Stem bark extract action towards M3 receptor in controlling blood glucose levels. Strychnos nux vomica  and Holarrhena pubescens are both alkaloidal drugs can help in controlling Hyperglycemic level. This will be useful in the formulation of a new herbal drug molecule for treating diabetes. Chloroform and ethanolic extracts of selected alkaloidal plants were extracted using the soxhlet apparatus and obtained quotes were tested for acute toxicity studies and carried out anti-diabetic action on Wister albino rats for 21 days. Results obtained from Blood glucose levels and histopathological study of test groups are compared with blood glucose levels of standard group, and highly significant action was identified by the chloroform extract of Strychnos nux vomica and Holarrhena pubescens group. Moderate anti-diabetic action was observed remaining two groups of ethanolic extracts. Strychnos nux vomica and Holarrhena pubescens ethanolic extract groups are acting on M3 receptors and controlling Hyperglycemic levels.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Taxithelium napalense acts against free radicals and diabetes mellitus

2017 ◽  
Vol 12 (2) ◽  
pp. 16 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Kishore Naidu Killari ◽  
Vedula Girija Sastry ◽  
Alekhya Ketha
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Video Clip ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Radical Scavenging Properties ◽  
Histopathological Studies

<p class="Abstract">In this work, <em>Taxithelium nepalense</em> ethanolic extract and its fractions were evaluated for the antidiabetic activity in rat based on phytochemical and free radical scavenging properties. The ethanolic extract, fraction IV and V significantly attenuated the blood glucose levels at 600, 200 and 200 mg/kg with 50.0, 33.5 and 42.0% inhibition respectively. The histopathological studies were manifesting the recuperation of damaged cells in liver and pancreas tissues. The outcomes of the present work affirm that the <em>T. nepalense</em> has a potency to plummet the overproduction of free radicals and blood glucose levels in the diabetic-induced rat.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 50 sec   <a href="https://youtube.com/v/GUZp4QY9kCs">Full Screen</a>   <a href="https://youtube.com/watch?v=GUZp4QY9kCs">Alternate</a></p>

Skeletal muscle sodium and potassium changes after successful surgery in acromegaly: relation to body composition, blood glucose, plasma insulin and blood pressure

1993 ◽  
Vol 128 (5) ◽  
pp. 418-422 ◽  
Author(s):  
Kerstin Landin ◽  
Björn Petruson ◽  
Karl-Erik Jakobsson ◽  
Bengt-Åke Bengtsson
Keyword(s):  
Blood Pressure ◽  
Skeletal Muscle ◽  
Body Composition ◽  
Blood Glucose ◽  
Plasma Insulin ◽  
Sodium Content ◽  
The Body ◽  
Potassium Content ◽  
Insulin Levels ◽  
Total Body

The aim of this study was to investigate the skeletal muscle sodium/potassium (Na/K) ratio in acromegaly before and 1 year after trans-sphenoidal removal of a growth hormone (GH)-secreting pituitary adenoma. Muscle biopsies were taken and skeletal muscle electrolytes, body composition, glucose, insulin and blood pressure were studied. Fasting blood glucose and plasma insulin levels, but not blood pressure, were higher in acromegalic patients (N = 9) than in controls (N = 6). The skeletal muscle potassium content was higher (p <0.01) but the sodium content and the Na/K ratio were lower (p<0.05 and p<0.001, respectively) in untreated patients with acromegaly as compared to weight-matched healthy controls. Elevated GH, glucose and insulin levels normalized after surgery. Blood pressure remained unchanged. The total body potassium content, the lean body mass and the total body water content decreased and the body fat content increased while the body weight was unchanged. The skeletal muscle potassium content decreased from [median (range)] 9.8 (9.2–11.5) to 7.7 (5.7–9.5) mmol/100 g wet wt (p<0.001). The skeletal muscle sodium content increased from 2.8 (2.5–3.9) to 5.1 (4.3–6.7) mmol/100 g wet wt (p<0.001) and the Na/K ratio increased from 0.28 (0.26–0.38) to 0.56 (0.51–1.18) (p< 0.001) after surgery, which is a higher level than the controls with a Na/K ratio of 0.47 (0.39–0.84) (p<0.01). These changes seem to be mediated by a decreased GH effect on the Na/K pump after successful trans-sphenoidal surgery in acromegaly.

scholarly journals A Scientific Validation of Antihyperglycemic and Antihyperlipidemic Attributes of Trichosanthes dioica

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Prashant Kumar Rai ◽  
Sharad Kumar Gupta ◽  
Amrita Kumari Srivastava ◽  
Rajesh Kumar Gupta ◽  
Geeta Watal
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Diabetes Management ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Postprandial Glucose ◽  
Tolerance Test ◽  
Antidiabetic Activity ◽  
Traditional Use ◽  
Trichosanthes Dioica

The present study was undertaken to scientifically validate the antidiabetic activity of aqueous fruit extract of Trichosanthes dioica Roxb. (Family: Cucurbitaceae) which has been traditionally used for managing diabetes mellitus. This plant commonly known as “Sespadula” in English has not been explored scientifically so far for its glycemic potential except by our research group. The study was conducted with variable doses on normal, mild, and severe diabetics models, and several biochemical parameters including blood glucose level (BGL) were assessed. Maximum fall in BGL of 23.8% in normal rats and of 31.3% in mild diabetic rats was observed during their fasting blood glucose (FBG) and glucose tolerance test (GTT) with the dose of 1000 mg kg−1. In severely diabetic animals after 4 weeks treatment with FBG, postprandial glucose, total cholesterol, and triglyceride levels were reduced by 28.7, 30.7, 57.2, and 18.5%, whereas high density lipoprotein, total protein, hemoglobin, and body weight were increased by 33.0, 36.7, 15.7 and 16.7%, respectively. Moreover, urine sugar was reduced from +4 to +1. Thus, the study scientifically validates the traditional use of T. diocia in diabetes management and could be developed as an effective oral agent for treating diabetes mellitus and complications associated with it.

Anti-Diabetic Potential of Murraya Koenigii (L.) and its Antioxidant Capacity in Nicotinamide-Streptozotocin Induced Diabetic Rats

Drug Research ◽  
2018 ◽  
Vol 68 (11) ◽  
pp. 631-636 ◽  
Author(s):  
Fauzul Husna ◽  
Franciscus Suyatna ◽  
Wawaimuli Arozal ◽  
Erni Poerwaningsih
Keyword(s):  
Blood Glucose ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
The Body ◽  
Antidiabetic Activity ◽  
Murraya Koenigii ◽  
Insulin Mimetic ◽  
Protein Levels ◽  
Glucose Levels

Abstract Aim and Objective The present study aims to investigate whether the antihyperglycemic effect of Murraya koenigii is mediated by antioxidant properties and insulin mimetic effect. Methods Thirty Spraque-Dawley rats were induced hyperglycemia by streptozotocin and nicotinamide (STZ-NA). The STZ-NA diabetic rats were treated with an ethanolic extract of Murraya koenigii 200 mg/kg b.w and 400 mg/kg b.w. One group was treated with glibenclamide (1 mg/kg b.w). After the administration of Murraya koenigii extract and glibenclamide for four weeks, the rats were sacrificed. Blood and organ samples were collected under a fasting condition. The body weight and blood glucose levels were measured. Hepatic enzymes were determined using a commercial kit, protein levels were estimated by Bradford’s method, and plasma insulin was assayed by an ELISA kit. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were estimated by the TBA-Wills method and Ellman’s method, respectively. Results Ethanolic extract of Murraya koenigii showed a significant reduction in blood glucose level at both doses, 200 and 400 mg/kg b.w. In addition, Murraya koenigii exhibited a profound antioxidant effect with decreased MDA level and increased GSH level, particularly at the 200 mg/kg b.w. and significantly decreased the HOMA-IR index. Conclusions The present study reveals that Murraya koenigii possesses antidiabetic activity and antioxidant effects on STZ-NA induced diabetes mellitus.

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