scholarly journals Identification and Characterization of Natural Habitats of Electrochemically Active Bacteria

Author(s):  
Yana Mersinkova ◽  
Hyusein Yemendzhiev

Aims: This study aims to define criteria for the main physical and chemical characteristics of the environmental niches populated with electrochemically active microorganisms, capable to perform anaerobic respiration and potentially used in Bio-electrochemical systems such as Microbial Fuel Cells.   Study Design: In this study, specific parameters of the environment in water bodies (such as lakes, streams etc.) and their bottom layers are analyzed. The main parameters of interest include the concentration of dissolved oxygen in the water column, the organic matter content in the sediments and the presence of alternative electron acceptors (such as iron and manganese ions) to support anaerobic respiration. Sediment microorganisms are characterized for their electrochemical and biodegradation activity. Place and Duration of Study: The tested sediment and water samples were collected from "Poda" Protected Site located on the outfall of Lake “Uzungeren”, south of City of Burgas, Bulgaria. Methodology: The samples were analyzed employing TGA, ICP and microbiological methods focusing on chemical, physical and biological conditions available for anaerobic respiration in this ecological niche. Results: The results show very low concentrations of dissolved oxygen (from 1.4 to 2.2 mg/dm3 in the various locations). The conductivity and the pH values ​​measured were relatively high and the mean values obtained are 5230 μS/cm and 8.2 respectively. The sediment samples demonstrated very high organic matter content (22.5% of the dry mass) and relatively high levels of iron and manganese. Microbial fuel cell powered by mixed bacterial culture isolated from the tested sediment samples demonstrated stable performance reaching power density of 3.5 W/m2 and the COD removal rate of 42 mgO2/dm3 per day. Conclusion: The result confirms the initial hypothesis that electrochemically active microorganisms are available in environments with high concentration of organic matter, iron and manganese in combination with low availability of dissolved oxygen. Mixed culture of anaerobic bacteria isolated from the tested sediment sample was successfully implemented to power Microbial Fuel Cell.

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
J. Gabriel Kuk-Dzul ◽  
Victoria Díaz-Castañeda

This study describes the relationship between mollusks, physicochemical properties of seawater, and sediments under natural conditions of low impact. Thirty-nine stations were sampled in October 1994 using a Van Veen grab (0.1 m−2). Temperature, salinity, and dissolved oxygen (DO) concentrations of bottom water were obtained with a CTD. Organic matter content and sediment grain analysis were determined. A total of 836 mollusks were collected. Gastropoda was the most abundant (52%) and diverse class with 27 genera, followed by Bivalvia with eight genera and Scaphopoda with only one genus. According to CCA analysis, dominant mollusks were significantly related with high DO concentrations.Donax,Natica,Acteocina,Bulla,Anachis,Odostomia, andCrucibulumcan be classified as sensitive genera because they were found mainly in high oxygen concentrations (3.1–5.6 mL L−1); on the other hand,Cardiomya,Nuculana,Laevicardium,Chione,Truncatella, andDentaliumcan be classified as tolerant genera (1.0–5.6 mL L−1). Todos Santos Bay hosts a diverse malacological fauna (36 genera); our results show that the dominant genera were mainly related to high dissolved oxygen concentrations. Mollusks can be a useful tool in environmental monitoring programs related with oxygen depletion in coastal areas.


2020 ◽  
Vol 9 (4) ◽  
pp. 423-430
Author(s):  
Gadisza Asmara Yudha ◽  
Chrisna Adhi Suryono ◽  
Adi Santoso

Pantai Kartini merupakan salah satu kawasan wisata di Kabupaten Jepara. Berbagai aktifitas masyarakat serta infrastruktur perairan menjadi kebutuhan yang sangat krusial dengan letak Pantai Kartini. Adanya aktivitas dan bangunan dapat menyebabkan perubahan sebaran ukuran butir dan kandungan bahan organik. Penelitian ini memiliki tujuan untuk mengetahui jenis dan klasifikasi sedimen serta jumlah kandungan bahan organik yang terdapat dalam sedimen di Pantai Kartini, Jepara. Pengambilan data pada penelitian ini yaitu pengambilan data primer yang berupa sampel sedimen dengan menggunakan sediment core. Dilanjutkan dengan analisis sampel sedimen dan analisis kandungan bahan organik. Hasil penelitian menunjukkan bahwa di perairan Pantai Kartini, Jepara memiliki jenis sedimen pasir (sand) dimana nilai ukuran butir tersebut berkisar dari 81-96%, yang mana pada Stasiun 1 rata-rata sebesar 91,6-96%, Stasiun 2 rata-rata sebesar 93,5-96,9%, dan Stasiun 3 rata-rata sebesar 81,4-92,9%. Sedangkan, Kandungan Bahan Organik yang terdapat di perairan tersebut memiliki konsentrasi berkisar dari 5-14% yang termasuk dalam kriteria rendah-sedang, dimana pada Stasiun 1 rata-rata sebesar 10,9-13,3%, Stasiun 2 rata-rata sebesar 5,92-10,18%, dan Stasiun 3 rata-rata sebesar 7,7-14%. Kartini Beach is one of the tourist areas in Jepara Regency. Various community activities and water infrastructure have become crucial needs in Kartini Beach. The existence of activities and buildings can cause changes in the distribution of grain size and content of organic matter. This study aims to determine the type and classification of sediments and the amount of organic matter content contained in sediments in Kartini Beach, Jepara. Data collection in this study is primary data collection in the form of sediment samples using sediment cores. Followed by analysis of sediment samples and analysis of organic matter content. The results showed that in Kartini Coast waters, Jepara had a sand sediment type where the grain size values ranged from 81-96%, which at Station 1 averaged 91,6-96%, Station 2 on average amounted to 93,5-96,9%, and Station 3 averaged 81,4-92,9%. Meanwhile, the content of organic matter contained in these waters has concentrations ranging from 5-14% which are included in the criteria of low-moderate, where at Station 1 an average of 10,9-13,3%, Station 2 an average of 5,92-10,18%, and Station 3 averaging 7,7-14%. 


Author(s):  
Olga Konechnaya ◽  
Christina Schwanen ◽  
Jan Schwarzbauer

Abstract The tremendous increase of plastic production, its intensive usage in packaging, as transport material and the insufficient management of plastic garbage lead to a rise of microplastic particles as anthropogenic contaminant in our environment. To develop appropriate management and remediation strategies for this global pollution problem, reliable and consistent analytical procedures for measuring plastics in the complex matrices need to be designed. The applicability of an easy, robust and fast multi-step approach was tested on three sediment samples from riverine, beach and backwater areas of varying origin, grain size and organic matter content is reported here. The optimized method included grain size fractionation, density separation and μ-FTIR analyses. Identification was based on two complementary methods of μ -FTIR measurements, the Image mode for small microplastics (<1 mm) and the ATR method for bigger (1–5 mm) particles. The analyses revealed the identification of several polymers in various grain sizes at different pollution levels. Major findings are the dominance of PET particles and the highest frequency of microplastic particles in the midsize fraction of 100–500 μm. Generally, the method was able to reliably detect microplastic particles in several grain size fractions and down to very low contamination levels of approximately. ten particles per 50 g of sediments with different organic matter content and various grain size characteristics. Moreover, the presented multi-step approach represents a fast, easy and less cost-effective method as alternative to more expensive and time-consuming methods.


2020 ◽  
Vol 32 ◽  
Author(s):  
Gustavo Ruschel Lopes ◽  
Hugo Mendes de Oliveira ◽  
Gabriel Fernandes Alves de Jesus ◽  
Maurício Laterça Martins ◽  
Carlos Henrique Araújo de Miranda Gomes ◽  
...  

Abstract: Aim The increment of decomposition of organic matter in sediment samples from Nile tilapia farms was evaluated with the introduction of Bacillus subtilis and B. licheniformis bacteria. Methods Sediment samples placed in 18L plastic boxes received single dose inoculum with the following concentrations: 1.21 x 106 CFU g-1 (equivalent to 75 g ha-1), 2.41 x 106 CFU g-1 (equivalent to 150 g ha-1), 4.82 x 106 CFU g-1 (equivalent to 300 g ha-1) and 1.61 x 107 CFU g-1 (equivalent to 1000 g ha-1), in addition to a control treatment with saline solution only. Organic matter content, total organic carbon (TOC) and oxidizable (OOC), total nitrogen (TN), ratios (TOC: N and OOC: N), clay content, pH in water, Shoemaker, McLean, Pratt index (SMP Index), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) contents, potential acidity (H + Al), cation exchange capacity (CEC) at pH 7.0, base saturation (V) and sum of bases (S). Results The values of OM showed significant difference, between the lowest values (treatments 75, 150 and 300 g ha-1) and the highest value, (control treatment). TOC, OOC, NT and their relationships (TOC: N and OOC: N) showed significant differences between the mean values of the control treatment and the other treatments. Conclusions The addition of Bacillus subtilis and B. licheniformis bacteria increased the decomposition rate of organic matter in sediments samples from Nile tilapia farms.


2021 ◽  
Vol 10 (2) ◽  
pp. 143-150
Author(s):  
Jamaludin Jamaludin ◽  
Sri Sedjati ◽  
Endang Supriyantini

Betahwalang is a coastal village located in Demak Regency. As a village located in a coastal area, the local population mostly relies on the fisheries sector sourced from sea products. Betahwalang Village has a Mangrove Ecosystem, which is included in the tidal river area of Betahwalang Village. This study aims to look at the comparison of organic matter levels in sediments during tidal and receding river water. Sampling in the field was conducted in March 2019. Sediment samples were taken from 5 stations divided into jetty, mangrove, estuary, and beach areas, where each station was repeated 3 times during high tide and low tide. Analysis of organic matter content of sediment samples using gravimetric methods and data analysis using Non-Parametric Kruskal-Wallis H. The results showed the highest levels of sediment organic matter at station 5 were 74.87 ± 1.81% (low tide) and 66.99 ± 0.38% (high tide) and the lowest sediment organic matter content. at station 4 were 31.56 ± 2.14% (low tide) and 26.93 ± 2.51% (high tide). These results indicate that the organic matter content in the sediment at low tide is higher than at high tide with results that are not significantly different (sig = 0.05).  Betahwalang adalah desa pesisir yang terletak di Kabupaten Demak. Sebagai desa yang terletak di wilayah pesisir, penduduk setempat sebagian besar mengandalkan sektor perikanan yang bersumber dari hasil laut. Desa Betahwalang memiliki Ekosistem Mangrove yang termasuk dalam wilayah sungai pasang surut di Desa Betahwalang. Tujuan dari penelitian ini adalah untuk melihat kondisi perairan sungai Betahwalang ditinjau dari kandungan bahan organik dalam sedimen selama pasang dan surut. Pengambilan sampel di lapangan dilakukan pada bulan Maret 2019. Sampel sedimen diambil dari 5 stasiun yang dibagi menjadi area dermaga, mangrove, muara dan pantai, di mana setiap stasiun diulang 3 kali selama pasang dan surut. Analisis kandungan bahan organik sampel sedimen menggunakan metode gravimetri, dan analisis data menggunakan Non-Parametrik Kruskal-Wallis H. Hasil penelitian menunjukkan kadar bahan organik sedimen tertinggi di stasiun 5 sebesar 74,87 ± 1,81% (air surut) dan 66,99 ± 0,38 % (air pasang) dan  kandungan bahan organik sedimen terendah. di stasiun 4 sebesar 31,56 ± 2,14 % (air surut) dan 26,93 ± 2,51% (air pasang). Hasil ini menunjukkan bahwa kandungan bahan organik dalam sedimen pada saat air surut lebih tinggi daripada saat air pasang dengan hasil yang tidak berbeda nyata (sig=0,05). 


1997 ◽  
Vol 15 ◽  
Author(s):  
S. M. Hussain ◽  
V. Manivannan ◽  
V. Ragothaman

An attempt has been made to establish the sediment-ostracode relationship, in the gulf of Mannar, off Tuticorin, south east coast of India. 48 sediment samples were collected from 12 sampling stations. Twelve bottom sediment samples were collected once in three months, for a period of one year, so as to represent the four seasons (winter, summer, southwest Monsoon and northeast Monsoon). Calcium carbonate and organic matter content of each sample were correlated against the ostracode population recovered in the respective sample. An increase in the CaCO3 percentage and a decrease in the organic matter content generally favor a higher population size.


2017 ◽  
Vol 46 (2) ◽  
Author(s):  
Anna Czaplicka ◽  
Ewa Szarek-Gwiazda ◽  
Zbigniew Ślusarczyk

AbstractThe study determines the differences in Pb accumulation in sediments of dam reservoirs with respect to locations of the old river beds and their depth (deep Czorsztyn Reservoir and shallow Goczałkowice Reservoir in southern Poland). Parameters (grain size, organic matter content and reservoir depth) that may influence the spatial distribution of Pb concentrations in the sediments were analyzed. Based on the hierarchical cluster analysis, sediment samples from the reservoirs were ranked with respect to particle size distribution. We found differences in the spatial distribution of grain size and organic matter in the studied reservoirs, caused mostly by the topography of these reservoirs. The spatial distribution of Pb concentrations in the sediments of the Czorsztyn Reservoir (range 4.8-35.8 μg g


2001 ◽  
Vol 28 (1) ◽  
pp. 13 ◽  
Author(s):  
LUCIANO LAYBAUER ◽  
EDISON DAUSACKER BIDONE

This work detaches the importance of understanding the sedimentary dynamics in environmental researches. One hundred eighty seven bottom sediment samples from Guaíba Lake were collected in 1998. These samples were analyzed for texture and organic matter content. Those physical parameters associated to the lake’s morfodynamics show preferential sites to accumulate fine sediments and organic matter; those are potential geochemical carriers of pollutants. Four textural fields (A: 0-10%, B: 10-50%, C: 50-90% and D: 90-100%) based on the mud percentage (fraction <63µm) of the sediments were defined. They indicate a growing potential of pollutant sorption. Environmental assessment should prioritize sampling of fields C, and especially D, to the detriment of fields A and B. the textural map based on this approach, shows the division of Guaíba Lake in three great cells of fine sediments called, North cell, Central cell and South cell. Future environmental researches carry on the Guaíba Lake can use this tool to orient sediment sampling.


2021 ◽  
Vol 4 (2) ◽  
pp. 127-133
Author(s):  
Muhamad Razid ◽  
Bintal Amin ◽  
Efriyeldi Efriyeldi

This study was conducted in July-August 2020 by using sediment samples and macrozoobenthos in the southern part of Bengkalis Island, Riau Province. The aim of this study is to find out how much the organic matter content, the abundance of macrozoobenthos, and the correlation between the organic matter content of the sediments to the abundance of macrozoobenthos. This study used a survey method consisting of 3 stations with 3 transects in each station. The organic matter content of sediments revolved from 4.85 to 8.52% in the waters of the southern part of Bengkalis Island. The highest organic matter content was at station III, namely 8.52%, while the lowest organic matter content was at station II namely 4.85%. The abundance of organisms found at the three research stations was 4.04 to 5.96 ind/m2. The abundance average at all macrozoobenthic research stations was 4.93 ind/m2. The results of simple linear regression analysis of organic matter content and abundance of macrozoobenthos showed a very weak correlation


Author(s):  
O. A. Lipatnikova

The study of heavy metal speciation in bottom sediments of the Vyshnevolotsky water reservoir is presented in this paper. Sequential selective procedure was used to determine the heavy metal speciation in bottom sediments and thermodynamic calculation — to determine ones in interstitial water. It has been shown that Mn are mainly presented in exchangeable and carbonate forms; for Fe, Zn, Pb и Co the forms are related to iron and manganese hydroxides is played an important role; and Cu and Ni are mainly associated with organic matter. In interstitial waters the main forms of heavy metal speciation are free ions for Zn, Ni, Co and Cd, carbonate complexes for Pb, fulvate complexes for Cu. Effects of particle size and organic matter content in sediments on distribution of mobile and potentially mobile forms of toxic elements have been revealed.


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