scholarly journals Efficacy of Entomopathogenic Fungus Beauveria bassiana Isolates against Callosobruchus maculatus (Coleoptera: Chrysomelidae) under Controlled Conditions

Author(s):  
Mellon Kabole ◽  
Dora Kilalo ◽  
Maina Muiru ◽  
Danny Coyne

Callosobruchus maculatus FaB (Coleoptera: Bruchidae), is a major field-to-store post-harvest pest of in the tropics and worldwide.  They cause weight loss, decreased germination potential and reduction in commercial and aesthetic value as a result of physical contamination of grain by insects, eggs and excrement, decreased nutritional value. Entomopathogenic fungi have been employed in control of a number of storage pests and has been demonstrated to have potential in control of C. maculatus in cowpea during storage. Nine B. bassiana isolates were evaluated for effectiveness in controlling C. maculatus in cowpea grain under controlled laboratory conditions. Mortality of the bruchids was evaluated stepwise where the most effective isolate concentrations against C. maculatus were assessed. Initial assessment involved determination of the most effective concentration among the isolates following dilution. The isolates showed significant differences on the mortality of cowpea bruchids at the different concentrations. Isolate J35 had 98.2% mortality at 5 days after application during the first season and 88.3% during the second season, which was only lower to the formulated isolates BBC and BVT. The current study shows the pathogenic effectiveness of B bassiana on C. maculatus.

Author(s):  
Mellon Kabole ◽  
Dora Kilalo ◽  
Maina Muiru ◽  
Danny Coyne

Callosobruchus maculatus Fab. (Coleoptera: Bruchidae), is a major field-to-store post-harvest pest of in the tropics and worldwide.  They cause weight loss, decreased germination potential and reduction in commercial and aesthetic value as a result of physical contamination of grain by insects, eggs and excrement, decreased nutritional value. Entomopathogenic fungi have been employed in control of a number of storage pests. Nine B. bassiana isolates were evaluated for effectiveness in controlling C. maculatus in cowpea grain under controlled laboratory conditions. Mortality of the bruchids was evaluated stepwise where the most effective isolate concentrations against C. maculatus were assessed. Initial assessment involved determination of the most effective concentration among the isolates following dilution. The most effective isolate concentrations were then doubled and halved to identify the most effective dose rate. The isolates showed significant differences on the mortality of cowpea bruchids at the different concentrations. The halved rate from the most effective rate with BBC recording 85% and 76.7 % cumulative mortality at day 3 during the first and second season respectively.  Isolate J35 had 98.2% mortality at 5 days after application during the first season and 88.3% during the second season, which was only lower to the formulated isolates BBC and BVT. At the optimal dose rate experiment, isolate J57 presented the highest cumulative mortality (25.5%) at 14 days after application while J59 recorded the lowest cumulative mortality at 11.8% at the same time. The combination of isolate J39 and RI showed the highest cumulative mortality of 40.4% and 44.4% during the first and second season respectively. Results obtained from this study support the importance of exploring the use of B. bassiana in control of C. maculatus during storage.


2018 ◽  
Vol 1 (2) ◽  
pp. 5
Author(s):  
Shankar Gopinat

Acute cervical facet fractures are increasingly being detected due to the use of cervical spine CT imaging in the initial assessment of trauma patients. For displaced cervical facet fractures with dislocations and subluxations, early surgery can decompress the spinal cord and stabilize the spine. For patients with non-displaced cervical facet fractures, the challenge in managing these patients is the determination of spinal stability. Although many of the patients with non-displaced cervical facet fractures can be managed with a cervical collar, the imaging needs to be analyzed carefully since certain fracture patterns may be better managed with early surgical stabilization.


2017 ◽  
Vol 19 (1) ◽  
pp. 31-35
Author(s):  
Frieda Rosita Majid ◽  
Nur Hidayat ◽  
Waluyo Waluyo

Background:Moringa (Moringaoleifera Lam.) Is a plant of high nutritional value, grows scattered in the tropics and sub-tropics, but utilization is still low. Each section has its benefits Moringa one part is the Moringa leaves contain a high calcium. In 100 grams of material, fresh Moringa leaves contain as much as 440 mg of calcium in the form of flour whereas if it contains as much as 2,003 mg of calcium. One of its use in the manufacture of flakes added. Objective: Know the difference physical harateristi, organoleptic characteristic and calcium levels in flakes variations addition of Moringa leaf powder. Methods: The study is a randomized experimental design with simple, includes four kinds of treatments, two replications with two experimental units. Observations of physical characteristic were analyzed by descriptive, the organoleptic characteristic of data analysis using statistical test Kruskal-Wallis continued Mann-Whitney and methods of test calcium content using permanganometri then the data were analyzed descriptive. Results: The physical characteristics of flakes greenish-yellow, slightly fragrant aroma typical of flakes, rather unpleasant taste typical of Moringa leaves and a slightly crunchy texture. Organoleptic characteristics the color of flakes with moringa leaf powder 5%, the aroma of the flakes without addition moringa leaf powder, the flavour of flakes with additions moringa leaf powder 5% and the texture of flakes with additions moringa leaf powder 7,5% most prefered panelist and high levels calcium of flakes with additions 10% moringa leaf powder. Conclusion: There is a difference variations addition of moringa leaf powder on physical, organoleptic characteristic (color) and the level of calcium flakes.Flakes with the addition of 5% moringa leaf powder is the most prefered panelist.   Keywords:Moringa Leaf Flour, Flakes, Physical characteristic, organoleptic, Calcium


Author(s):  
B.G. Tsugkiev ◽  
◽  
L.Ch. Gagieva ◽  
T.V. Tsugkieva ◽  
M.M. Chegaev ◽  
...  

The paper presents a comparative assessment of the nutritional value depending on the phenological phase of Onobrychis viciifolia Scop., as well as the determination of the regularity of the accumulation of biologically active substances and heavy metals in the herb of the species. The yield of green mass of plants was determined. From each hectare of sowing, 9,7 centners of protein can be obtained; the yield of fiber is 13 centners, BEV is 22,9 centners, fat is 1,6 centners, ash is 4 centners and carotene is 13.0 kg. Depending on the growing season, the chemical composition in the grass of Onobrychis viciifolia changes. The content of calcium, phosphorus increases, and the presence of iron, copper, cobalt, zinc, manganese and molybdenum and amino acids decreases.


Author(s):  
M.L. Schulman ◽  
J.P. Nurton ◽  
A.J. Guthrie

The most useful diagnostic methods in the initial evaluation of horses with colic assess the morphological and functional status of the gastrointestinal tract and cardiovascular status. This evaluation is best achieved using a combination of clinical and laboratory data. Blood lactate concentration (BL) is one of these variables. BL rises mainly due to poor tissue perfusion and anaerobic glycolysis associated with shock, providing an indicator of both the severity of disease and its prognosis. A hand-held lactate meter, Accusport, provides a rapid (60 seconds), inexpensive dry-chemical-based determination of BL. This trial evaluated the Accusport's ability to provide BL data as an adjunct to the initial clinical evaluation of horses with colic. The accuracy of the Accusport was tested by evaluation of its interchangeability with the benchmark enzymatic kit evaluation of BL in a trial using data collected firstly from 10 clinically normal control horses and subsequently from 48 horses presented with signs of colic. The BL values were recorded together with the clinical variables of heart rate (HR), capillary refill time (CRT), haematocrit (Hct), and pain character and severity on the initial assessment of the colic horses. Information regarding choice of therapeutic management (medical or surgical) and eventual case outcome (full recovery or died/euthanased) was recorded. The Accusport was found to be interchangeable with the enzymatic kit for recording BL values in colic horses with BL <10 mmol/ , which is within the BL range associated with survival. The interchangeability of an additional, laboratory-based wet chemical assay for BL, the Stat 7 was simultaneously evaluated for the colic and control horses. The Stat 7 was found to be interchangeable with the enzymatic kit for BL determination of colic horses. No linear associations between BL values with HR, CRT, Hct or pain assessment were observed. No relationship with either selection of therapeutic method or eventual case outcome was observed. All horses with BL >8 mmol/ died or were euthanased.


2018 ◽  
Vol 6 (4) ◽  
pp. 208
Author(s):  
Hamdiana Hamdiana ◽  
Minarni Rama Jura ◽  
Ratman Ratman

The use of traditional materials is one alternative for the treatment of kidney stones. One of them is utilizing a plant of patikan kebo (Euphorbia hirta). This study is conducted by using laboratory experiments and the extract of the plant of patikan kebo red and patikan kebo green to dissolve the calcium phosphate as a substitute for kidney stones. The extraction of samples is conducted by decoction. The aim of the study is to determine the effective concentration of the extracts of patikan kebo red and green to dissolve calcium and test the effectiveness of the extracts of patikan kebo to dissolve calcium compared to Batugin elixir and distilled water. Determination of the concentration of dissolved calcium in the extracts using a flame photometer. The results show that the extract of patikan kebo red with a concentration of 2.1% is effective in dissolve the calcium with a concentration of 5.2 ppm. The extract of patikan kebo green with a concentration of 27.4% is effective in dissolving the calcium with a concentration of 27.5 ppm. When compared with Batugin elixir and distilled water, Batugin elixir can only dissolve the calcium with concentration 15.3 ppm, while distilled water can only dissolve 3 ppm of the calcium.


2015 ◽  
Vol 4 (5) ◽  
pp. 215-221
Author(s):  
Ablaye Faye ◽  
Malick Sarr ◽  
Abdoulaye Samb ◽  
Cheikh Thiaw ◽  
Mbacké Sembène

  The effect of neem leaves has been tested in the laboratory on eggs and adults of cowpea weevil (C. maculatus). Different formulations of this plant were applied to these forms of C. maculatus Fab. Grinding fresh contact sheets induced significant lethal effects from 96.12% to 100% on eggs; whereas 100% of mortality was recorded at the end of eight days of applica-tion to three adults with higher doses. Fumigation on turn proved less effec-tive than contact on eggs. It induced a maximal effect of 95.73% mortality with the larger dose (D4: 0.02912g/cm3). On adults, we recorded highest mortality (100%) from the 7th day of the show with the highest dose. The aqueous extract of neem leaf powder was less effective than all other formu-lations on the eggs as well as adults of this insect; with a higher mortality rate (74.99%) observed on the eggs with the application of the concentration C2. On adults we recorded a maximum effect (100% mortality) from the 13th day of the application with the highest concentration (C1). These mortalities would be related to the support of several active molecules contained in neem as established in literature.


2020 ◽  
Vol 161 ◽  
pp. 01106
Author(s):  
Lyudmila Rodionova ◽  
Irina Sobol ◽  
Lyudmila Donchenko ◽  
Natalia Limareva

The article presents the results of studies of changes in the vitamin composition of pumpkin, grown in the Kuban region. Standard and modern methods of physical and chemical analysis were used in the research. It is shown that the nutritional value of this raw material allows to maintain its nutritional value for a long time. Differential determination of carotenoid pigments contained in pumpkin fruits was carried out. The quantitative characteristics of carotenoids were established and the predominant carotenoid pigments for the studied pumpkin varieties were specified.


1999 ◽  
Vol 1999 ◽  
pp. 146-146
Author(s):  
S. A. Abdulrazak ◽  
T. Awano ◽  
T. Ichinohe ◽  
T. Fujihara ◽  
J. Nyangaga

Tree legume forages plays an important role in livestock nutrition in many parts of the tropics. One of the commonly used tree species is leucaena. However, the recent infestation of Leucaena leucocephala by the pest Heteropsylla cubana calls for alternative sources of legume forages. Abdulrazak et al. (1997) for instance, showed that Gliricidia sepium could be an alternative, and indicated the need to identify more promising species. Prosopis Juliflora (prosopis) grows in many parts of the tropics, including the arid and semi arid areas of Kenya. The objective of this study is to evaluate the nutritional value of prosopis fruits (pods with seeds) and leaves as ruminant feed.


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