scholarly journals Analgesic Effects of Vigna unguiculata subsepecies dekindtiana in Mice

2020 ◽  
pp. 10-22
Author(s):  
Adekunle T. Adegbuyi ◽  
Moses A. Akanmu ◽  
G. Olayiwola ◽  
Abayomi O. Sijuade
Keyword(s):  
Acetic Acid ◽  
Receptor Antagonist ◽  
Vigna Unguiculata ◽  
Analgesic Effect ◽  
Methanol Extract ◽  
Positive Control ◽  
Hot Plate ◽  
Thermal Tests ◽  
Writhing Test

In the present study, we investigated the antinociceptive effects of the plant Vigna unguiculata spp dekindtiana using chemical and thermal tests in mice. The peripheral and the central analgesic activities of the methanol extract and its fractions were investigated in-vivo in albino mice using acetic acid induced-writhing test and hot plate models respectively. The result of the central analgesic effect showed that the methanol extract (VUME) at 400 mg/kg produced a significant (p<0.05) delay in reaction time in mice on hot plate compared to the control. Various fractions of the extract showed more potency compared to the crude extract. In acetic writhing model, the extract and the fractions demonstrated dose dependent reduction in writhing reaction induced by acetic acid in mice. The reduction was significant when compared to control which was suggestive of the analgesic effect of the plant. It was also seen that the extract and fractions showed an improved analgesic effect compared to diclofenac used as positive control in this model. Yohimbine (alpha adrenergic receptor antagonist) and cyproheptadine (serotonergic receptor antagonist) reversed the antinociceptic effect of the fractions in the hot plate model demonstrating the possibility of adrenergic and serotonergic involvement in eliciting the analgesic effect. Naloxone on the other hand, caused a reversal only in the butanol fraction meaning that this fraction may contain active principles that may mediate their analgesic effect through the opioid mechanism. In the writhing test, yohimbine abolished the analgesic effect of both hexane and butanol fractions. This may therefore, suggest that the analgesic effect of these fractions may be mediated through adrenergic pathway. In conclusion, the plant V. unguiculata subspecies dekindtiana possesses active principles with potential analgesic activity, establishing the folkloric use of the plant in managing pain.

scholarly journals Preliminary assessment of the anti-inflammatory and analgesic effects of methanol leaf extract of Cussonia barteri (Araliaceae) in rodents

2019 ◽  
Vol 65 (3) ◽  
pp. 22-31
Author(s):  
Ighodaro Igbe ◽  
Osaze Edosuyi ◽  
Agbonlahor Okhuarobo ◽  
Adarki Pongri ◽  
Nkechi Maduako ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Tail Flick ◽  
Hot Plate ◽  
Analgesic Effects ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Ear Oedema

Summary Introduction: Potato (Solanum tuberosum L.) is an important vegetable crop in Syria. Potato tuber moth Cussonia barteri is a small tree that grows in the sub-Saharan part of Africa. Various parts of the plant are used for the treatment of a variety of ailments in ethno-medicine. Objective: To evaluate the anti-inflammatory and analgesic effect of the methanol leaf extract of Cussonia barteri. Material and methods: The leaves were air-dried, powdered and repeatedly extracted with methanol using a Soxhlet apparatus. The resulting methanol extract (100, 200 and 400 mg/kg) was evaluated for anti-inflammatory activity using carrageenan-induced paw oedema, xylene-induced ear oedema and formalin-induced arthritis tests. Analgesic effect was evaluated using acetic acid-induced mouse writhing, hot plate and tail flick tests. Results: All doses of the extract significantly (p<0.05) reduced carrageenan-induced paw oedema, however the 400 mg/kg dose gave a sustained effect. The extract significantly inhibited xylene induced ear oedema at all doses. There were no significant (p>0.05) reductions in paw swellings due to formalin. In the acetic acid induced writhing test, the extract significantly (p<0.05) decreased writhing at 400 mg/kg only. Reaction times were not significantly different from the control in the hot plate and tail flick tests. Conclusion: This study has shown that the methanol extract possesses acute anti-inflammatory and peripherally mediated analgesic effects.

Antinociceptive Effect of Clomipramine Through Interaction with Serotonin 5-Нт2 And 5-Нт3 Receptor Subtypes

Folia Medica ◽  
2012 ◽  
Vol 54 (4) ◽  
pp. 69-77 ◽  
Author(s):  
Ilia D. Kostadinov ◽  
Delian P. Delev ◽  
Ivanka I. Kostadinova
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Antinociceptive Effect ◽  
Positive Control ◽  
Control Group ◽  
Receptor Subtypes ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Acid Test

Abstract INTRODUCTION: Tricyclic antidepressants are used in the treatment of various pain syndromes. The antidepressant clomipramine inhibits predominantly the reuptake of serotonin in the central nervous system. The mechanism of its analgesic effect is not fully understood. The AIM of the present study was to find experimentally any dose-effect dependence in the analgesic effect of clomipramine and the involvement of the 5-НТ2 and 5-НТ3 receptors in the mechanism of this effect. Material and methods: Fifty male Wistar rats were used in the study allocated to five groups (10 animals each): a saline treated control group, one positive control group treated with metamizole and three experimental groups treated with intraperitoneally administered clomipramine in doses of 5, 10 and 20 mg/kg bw, respectively. To study the role of 5-НТ2 and 5-НТ3 receptors in this effect we used another five groups (10 animals each): control, positive control and three experimental groups treated with clomipramine only, clomipramine and granisetrone and clomipramine and cyproheptadine, respectively. Three nociceptive tests were used: the hot plate test, analgesimeter and the acetic acid-induced writhing test. To gauge the antinociceptive action we used the increased latency in the hot plate test expressed as maximum possible effect % (%MPE), the increase in paw pressure to withdraw the hind paw in analgesimeter and decrease in the number of spinal cord writhes in the acetic acid test. RESULTS: Clomipramine in a dose of 20 mg/kg bw significantly increased the %MPE in hot plate test and the pressure to withdraw the hind paw in the analgesimeter when compared with the control. In the acetic acid test clomipramine decreased non-significantly the number of writhes compared with the controls. Granisetrone reduced non-significantly the antinociceptive effect of clomipramine in all tests. Cyproheptadine potentiated the analgesic effect of clomipramine in acetic acid test and decreased it significantly in the hot plate test. In analgesimeter cyproheptadine decreased significantly the paw pressure to withdraw the tested hind paw at 1 hour and non-significantly at 2 hours. CONCLUSION: Clomipramine in the dose of 20 mg/kg bw has a pronounced antinociceptive affect towards thermal and mechanical pain stimulation. The 5-HT2 and 5-HT3 receptor subtypes are very likely involved in the mechanism of this effect.

scholarly journals In vivo Analgesic activity of methanolic extract of Helianthus annuus seeds

2016 ◽  
Vol 5 (4) ◽  
pp. 38-40 ◽  
Author(s):  
Rubab Tarannum Islam ◽  
Ahmed Tanjimul Islam ◽  
Mir Monir Hossain ◽  
Kishor Mazumder
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Helianthus Annuus ◽  
Analgesic Activity ◽  
Latency Period ◽  
Pharmaceutical Journal ◽  
Mice Model ◽  
Hot Plate ◽  
Writhing Test

The sunflower seed is the seed of the sunflower (Helianthus annuus). The methanol extract of seeds of Helianthus annuus were screened for analgesic activity in mice model to systematically explore the medicinal values of the plant. Acetic acid induced writhing and hot plate methods were used to confirm the central and peripheral analgesic action. In case of acetic acid-induced writhing test the extract showed significant (P <0.05) analgesic potential at doses 100 and 200 mg/kg body weight (50.35 and 57.85% inhibition, respectively). In the hot plate method, increase (p < 0.05) of latency period was also observed in comparison to standard aspirin. At 60 minutes, the latency period of two different doses (100 and 200 mg/kg body weight) was found at 13 ± 0.91 and 16.5 ± 1.55 second. The results obtained support the use of Helianthus annuus seeds in painful conditions acting both centrally and peripherally.Islam et al., International Current Pharmaceutical Journal, March 2016, 5(4): 38-40http://www.icpjonline.com/documents/Vol5Issue4/02.pdf

scholarly journals In Vivo Analgesic Activity of Omega-3 on Mice Induced Peripheral Pain

2019 ◽  
Vol 12 (2) ◽  
pp. 961-963
Author(s):  
Ana Khusnul Faizah ◽  
Yanu Andhiarto ◽  
Pramudita Riwanti
Keyword(s):  
Acetic Acid ◽  
Fish Oil ◽  
Analgesic Effect ◽  
Positive Control ◽  
Negative Control ◽  
Ulcer Bleeding ◽  
Male Mice ◽  
Omega 3 ◽  
Peripheral Pain

Fish oil which contains omega 3 with the most types of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) has the effect of pharmacology as anti-inflammatory, antioxidant and is good for heart disease where probably it can be used beneficially as an analgesic. Analgesics have several side effects including gastric ulcer, bleeding, even Steven Johson’s Syndrome, have been attributed to their use. This research aims to determine the analgesic effect of fish oil on male white mice induced to produced peripheral pain. We used acetic acid-induced male BALB/c white mice to investigate analgesic effect fish oil in vivo by Writhing method. From this research, fish oil decreased writhings number in mice dose 5, 10, 20 and 30 mg/kg compared to negative control (P<0.001). The percentage inhibition of writhing at the dose 30 mg/kg is the hignest among other dose (42.64%), while in the group positive control (acetylsalysilic acid 100 mg/kg) is 68.26%. We concluded that fish oil has analgesic effect which reduced writhings in male mice induced by acetic acid to produce peripheral pain.

scholarly journals Analgesic effect of the aqueous extract of Artimisia herba alba Arial part

2010 ◽  
Vol 9 (3) ◽  
pp. 28
Author(s):  
Sh. M. Al-khazrji , and I. K. Khalil
Keyword(s):  
Acetic Acid ◽  
Aqueous Extract ◽  
Cold Water ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Plate Test ◽  
Analgesic Effects ◽  
Rats And Mice ◽  
Dose Dependent

The present study was aimed to investigate the analgesic effects of the aqueous extract of Artemisia herba alba Arial part in rats and mice ( AEAHA ). The AEAHA (400- 700 mg/kg; p.o.) was evaluated for its analgesic activity by employing acetic acid-induced writhing test, hot plate test and tail immersion tests i.e. in hot and cold water. AEAHA (400- 700 mg/kg; p.o.) showed significant (P<0.01) reduction in the number of writhing induced by acetic acid,increased reaction time in hot plate test and elevated pain threshold in hot and cold water tests. AEAHA exhibited the dose-dependent analgesic effects

scholarly journals Preparation and Analysis of New 1,3,5-Trisubstituted-2-Pyrazolines Derivative for Their Analgesic Potential

2021 ◽  
pp. 153-164
Author(s):  
Jitendra Kumar Chaudhary ◽  
Alok Pal Jain ◽  
O. P. Tiwari
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Latency Time ◽  
Hot Plate ◽  
Phenyl Hydrazine ◽  
Hot Plate Test ◽  
Test Technique ◽  
Plate Test ◽  
Withdrawal Latency ◽  
In Series

The goal of the study was to develop, synthesise, and characterise a novel 1,3,5-trisubstituted-2-pyrazolines derivative, as well as to assess its analgesic potential. The reaction of chalcone derivatives with 4-hydrazinylbenzene sulfonamide hydrochloride and phenyl hydrazine hydrochloride yielded 1,3,5-tri-substituted-2-pyrazolines derivatives. The IR, 1HNMR, and mass spectrum analyses were used to characterise a total of sixteen substances. Analgesic activity of the proposed substances has been tested. The analgesic effect of the produced compounds was tested using two methods: the hot plate test technique and acetic acid induced writhing in mice. To compare the effectiveness, pentazocine and acetyl acetic acid were utilised as reference drugs. The hot plate test technique and acetic acid induced writhing in mice were used to assess the analgesic effect of the 16 produced chemical series A1-A8, and B1-B8. The evaluation's outcomes were viewed using Pentazocine and acetyl acetic acid as the standard drugs. In a 90-minute hot plate test, compounds A2 (10.30 s), A4 (9.45 s), A7 (11.65 s), and A8 (11.26 s) showed a delay in paw withdrawal latency time. Compounds B2 (9.10 s) and B7 (10.42 s) prolong the paw withdrawal latency time after 90 minutes in series B1-B8, reduce the pain feeling, and inhibit pain induced by heat methods. Compounds A2, A5, A6, A7, and A8 from Series A1-A8 showed 83.00, 76.01, 80.34, 86.99, 88.15 percent inhibition, substantially (p0.05 and p0.001, respectively), and decreased the number of wriths caused by 0.6 percent acetic acid at a dosage of 10 mg/kg. Acetylsalicylic acid (10 mg/kg) appears to be more successful in lowering the number of wriths, with a 99.0% reduction in the number of wriths (p0.001). B1, B3, and B4 have the least amount of active activity. These all finding suggest that these synthesized compounds have the potential as analgesic agent.

scholarly journals Analgesic and Antidiarrheal Activities of Leaf of Podocarpus neriifolius D. Don

2016 ◽  
Vol 19 (2) ◽  
pp. 215-218
Author(s):  
Md Rahatullah Razan ◽  
Muhammed Mahfuzur Rahman ◽  
Faiza Tahia ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Castor Oil ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Pharmaceutical Journal ◽  
Activity Assay ◽  
Swiss Albino Mice

The methanol extract of leaf of Podocarpus neriifolius D. Don exhibited in vivo peripheral analgesic and antidiarrheal activities in Swiss Albino mice. In the peripheral analgesic activity assay, the methanolic extract showed 50.00 ± 8.57% and 70.25 ± 1.18% inhibition of acetic acid-induced writhing at 200 and 400 mg/kg body weight, respectively. In addition, the extract also revealed a dose dependant inhibition of castor oil- induced diarrhea with 43.77 ± 3.13% and 56.23 ± 6.49% inhibition of feces at 200 and 400 mg/kg body weight, respectivelyBangladesh Pharmaceutical Journal 19(2): 215-218, 2016

scholarly journals In vivo and in silico evaluation of analgesic activity of Lippia alba

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Md. Abdullah Aziz ◽  
Manna Mehedi ◽  
Mst. Irin Akter ◽  
Sadiur Rahman Sajon ◽  
Kishor Mazumder ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
In Silico ◽  
Cyclooxygenase 2 ◽  
Latency Time ◽  
Hot Plate ◽  
Standard Drug ◽  
Lippia Alba ◽  
Tail Immersion

Abstract Background This study was conducted to evaluate the analgesic activity of different extracts of Lippia alba (L. alba) along with in silico evaluation of analgesic activity of the isolated compounds from L. alba against cyclooxygenase-2 enzyme and ADME/T analysis of isolated compounds. Method In vivo analgesic activity of different extracts of L. alba was evaluated by acetic acid-induced writhing, tail immersion and hot plate on Swiss albino mice of either sex. In silico activity of the isolated compounds and ADME/T analysis were performed by Schrödinger-Maestro (Version 10.1) and OSIRIS Data warrior (version 4.6.1) softwares. Results Three different extracts (Methanolic extract: ME; Petroleum ether extract: PEE; Dichloromethane extract: DCME) of 250 mg/kg and 500 mg/kg doses were used in the experiments to evaluate analgesic activity. In acetic acid-induced writhing test, significant results were seen for PEE (500 mg/kg) and DCME (500 mg/kg), which were 53.09 ± 2.87 & 50.09 ± 4.24%, respectively. In tail immersion test, the best latency time was found at + 60 min for PEE (500 mg/kg) which is (5.65 ± 0.25) sec. For hot plate test, DCME at a dose 500 mg/kg showed the highest increase in latency time, which was 13.48 ± 0.33 s. In the case of in silico evaluation of analgesic activity, the compounds such as geranial, neral, (E)-caryophyllene, caryophyllene oxide, mussaenide, and 8-epi-loganin meet the condition of Lipinski’s rule of five. Among these safe compounds, 8-epi-loganin showed the best docking score of − 8.17 kcal/mol against cyclooxygenase-2 enzyme (PDB ID: 6COX), which was almost similar to that of the standard drug, Celecoxib (− 11.11 kcal/mol). Conclusion In conclusion, L. alba can be a potent source of analgesic medicine and further modification and simulation studies are required to establish the effectiveness of 8-epi-loganin.

scholarly journals Analgesic and Anti-Inflammatory Activities of Resveratrol through Classic Models in Mice and Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Guangxi Wang ◽  
Zhiqiang Hu ◽  
Xu Song ◽  
Qiankun Cui ◽  
Qiuting Fu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Activities ◽  
Drug Candidate ◽  
Hot Plate ◽  
Sod Activity ◽  
Analgesic Effects ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Ear Oedema

Background. Inflammation and pain are closely related to humans’ and animals’ health.Resveratrol(RSV) is a natural compound with various biological activities. The current study is aimed to evaluate the analgesic and anti-inflammatory activities of RSV in vivo. Materials and Methods. The analgesic effects were assessed by the acetic acid-induced writhing and hot plate tests. The anti-inflammatory effects were determined using the xylene-induced mouse ear oedema, the acetic acid-induced rat pleurisy, and carrageenan-induced rat synovitis tests, respectively.Results. The analgesic results showed that RSV could significantly inhibit the number of writhes and improve the time and pain threshold of mice standing on hot plate. The anti-inflammatory results showed that RSV could inhibit the ear oedema of mice. In acetic acid-induced pleurisy test, RSV could significantly inhibit the WBC and pleurisy exudates, could decrease the production of NO, and elevate the activity of SOD in serum. In carrageenan-induced synovitis test, RSV could reduce the content of MDA and elevate the T-SOD activity in serum; RSV could inhibit the expressions of TP, PGE2, NO, and MDA.Conclusion. Shortly, these results indicated that RSV had potent analgesic and anti-inflammatory activities and could be a potential new drug candidate for the treatment of inflammation and pain.

scholarly journals UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN GEDI (Abelmoschus manihot (L.) Medik) PADA MENCIT (Mus musculus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Ristanti Pratiwi ◽  
Jimmy Posangi ◽  
Fatimawali .
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Maximum Effect ◽  
Control Group ◽  
Hot Plate ◽  
Thermal Pain ◽  
The Difference

Abstract: The objectives of the research were to find out the analgesic effect of giving Gedi (Abelmoschus manihot (L.) Medik) leaf ethanol extract orally on the number of writhing after thermal pain induction of mice. This research using 15 mice which is divided into 5 groups consisted of 1 negative control group given by the aquades, 1 positive control group given by the tramadol, and 3 experiment groups. Experiment group given by Gedi (Abelmoschus manihot (L.) Medik) leaf ethanol extract with the doses which different each other, that is 30 mg/30 g BW, 60 mg/30 g BW and 120 mg/30 g BW. Thermal pain induction was done by placing the mice on hot plate constant temperature of 550C. The mice gave respond in the way of lick its foot or even jumping. The data was collected using table, graphic and analyzed using one direction ANOVA model and it was continued with LSD test to find out the difference every treatment group. The result of analysis showed that gedi’s leaf ethanol extract have the analgesic effect and the maximum effect presented at gedi leaf ethanol extract dosage 60 mg/30 g BW. Keywords: Gedi’s leaf, analgesic effect    Abstrak: Tujuan penelitan ini yaitu menemukan efek analgesik dari pemberian ekstrak etanol daun gedi (Abelmoschus manihot (L.) Medik) peroral pada mencit yang kemudian diamati jumlah geliatnya setelah diinduksi panas. Penelitian ini menggunakan 15 ekor mencit yang dibagi 5 kelompok yang terdiri dari 1 kelompok kontrol negatif yang diberi aquades, 1 kelompok kontrol positif yang diberi tramadol, dan 3 kelompok eksperimen. Kelompok eksperimen diberi ekstrak etanol daun gedi dengan dosis yang berbeda-beda, yaitu 30 mg/30 g BB, 60 mg/30 g BB, dan 120 mg/30 g BB. Induksi nyeri berupa panas dilakukan dengan meletakkan mencit pada hot plate dengan suhu 550C . Mencit memberi respon berupa menjilat kaki dan atau melompat. Data disajikan berupa tabel, grafik dan menggunakan analisis statistik ANOVA yang dilanjutkan dengan LSD untuk menemukan perbedaan dari setiap kelompok. Hasil analisis menunjukkan bahwa ekstrak etanol daun gedi memiliki efek analgesik dan efek maksimumnya didapatkan pada dosis 60 mg/30 g BB. Kata kunci: Daun gedi, efek analgesik

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