scholarly journals In Vivo Analgesic Activity of Omega-3 on Mice Induced Peripheral Pain

2019 ◽  
Vol 12 (2) ◽  
pp. 961-963
Author(s):  
Ana Khusnul Faizah ◽  
Yanu Andhiarto ◽  
Pramudita Riwanti
Keyword(s):  
Acetic Acid ◽  
Fish Oil ◽  
Analgesic Effect ◽  
Positive Control ◽  
Negative Control ◽  
Ulcer Bleeding ◽  
Male Mice ◽  
Omega 3 ◽  
Peripheral Pain

Fish oil which contains omega 3 with the most types of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) has the effect of pharmacology as anti-inflammatory, antioxidant and is good for heart disease where probably it can be used beneficially as an analgesic. Analgesics have several side effects including gastric ulcer, bleeding, even Steven Johson’s Syndrome, have been attributed to their use. This research aims to determine the analgesic effect of fish oil on male white mice induced to produced peripheral pain. We used acetic acid-induced male BALB/c white mice to investigate analgesic effect fish oil in vivo by Writhing method. From this research, fish oil decreased writhings number in mice dose 5, 10, 20 and 30 mg/kg compared to negative control (P<0.001). The percentage inhibition of writhing at the dose 30 mg/kg is the hignest among other dose (42.64%), while in the group positive control (acetylsalysilic acid 100 mg/kg) is 68.26%. We concluded that fish oil has analgesic effect which reduced writhings in male mice induced by acetic acid to produce peripheral pain.

scholarly journals Analgesic Effects of Vigna unguiculata subsepecies dekindtiana in Mice

2020 ◽  
pp. 10-22
Author(s):  
Adekunle T. Adegbuyi ◽  
Moses A. Akanmu ◽  
G. Olayiwola ◽  
Abayomi O. Sijuade
Keyword(s):  
Acetic Acid ◽  
Receptor Antagonist ◽  
Vigna Unguiculata ◽  
Analgesic Effect ◽  
Methanol Extract ◽  
Positive Control ◽  
Hot Plate ◽  
Thermal Tests ◽  
Writhing Test

In the present study, we investigated the antinociceptive effects of the plant Vigna unguiculata spp dekindtiana using chemical and thermal tests in mice. The peripheral and the central analgesic activities of the methanol extract and its fractions were investigated in-vivo in albino mice using acetic acid induced-writhing test and hot plate models respectively. The result of the central analgesic effect showed that the methanol extract (VUME) at 400 mg/kg produced a significant (p<0.05) delay in reaction time in mice on hot plate compared to the control. Various fractions of the extract showed more potency compared to the crude extract. In acetic writhing model, the extract and the fractions demonstrated dose dependent reduction in writhing reaction induced by acetic acid in mice. The reduction was significant when compared to control which was suggestive of the analgesic effect of the plant. It was also seen that the extract and fractions showed an improved analgesic effect compared to diclofenac used as positive control in this model. Yohimbine (alpha adrenergic receptor antagonist) and cyproheptadine (serotonergic receptor antagonist) reversed the antinociceptic effect of the fractions in the hot plate model demonstrating the possibility of adrenergic and serotonergic involvement in eliciting the analgesic effect. Naloxone on the other hand, caused a reversal only in the butanol fraction meaning that this fraction may contain active principles that may mediate their analgesic effect through the opioid mechanism. In the writhing test, yohimbine abolished the analgesic effect of both hexane and butanol fractions. This may therefore, suggest that the analgesic effect of these fractions may be mediated through adrenergic pathway. In conclusion, the plant V. unguiculata subspecies dekindtiana possesses active principles with potential analgesic activity, establishing the folkloric use of the plant in managing pain.

scholarly journals Effects of Industrial Waste Fish Oil Administration on Interleukin-6 (Il-6) Expression at Mice Colon being Induced by Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS)

2021 ◽  
Vol 14 (4) ◽  
pp. 2227-2233
Author(s):  
Kevin Tjoa ◽  
Kusmardi Kusmardi ◽  
Yurnadi Hanafi Midoen
Keyword(s):  
Fish Oil ◽  
Industrial Waste ◽  
Positive Control ◽  
Negative Control ◽  
High Dose ◽  
Omega 3 ◽  
Colon Tissue ◽  
Significant Difference ◽  
Solvent Control ◽  
Medium Dose

Colorectal cancer (CRC) is the world’s third most cancer and the second highest mortality rate. The searching for new anti-inflammation substances with less adverse effects than aspirin for chemoprevention and adjuvant chemotherapy of CRC is running. The most notable one is fish oil containing omega 3. Kusmardi, et al. studied that industrial waste fish oil omega-3 level comes close enough to conventional fish oil industry. Study aims to reducing the level IL-6 on mice colon tissue being induced CRC using AOM/DSS by fish oil administration. Thirty male Swiss Webster mice are grouped into six treatments: positive control (aspirin), negative control (physiological saline), normal, high dose (fish oil 6mg/kgBW), medium dose (fish oil 3mg/kgBW), dan solvent control (corn oil). Colon tissue was stained using anti IL-6 antibody. Ten photos per slide were taken by microscope (400x), analyzed for the IL-6 expression by ImageJ®, and quantified for H-score. Data was analyzed using SPSS 24.0 (CI 95%) and p-value <0.05 is consider significant. Data are not normally distributed with median of 161.64 (119.4-260.67). Kruskal-Wallis test is significant in addition with Mann-Whitney test shows only high dose group has significant difference to negative control (p=0.008), medium dose (p=0.016) dan and solvent control (p=0.008). No significant difference reported between high dose and positive control group (p=0.69). High dose industrial waste fish oil can lower IL-6 expression in mice colon tissue induced CRC using AOM/DSS.

scholarly journals Efek Analgesik Ekstrak Etanolik Daun Mangrove Api-Api Putih (Avicennia alba) terhadap Mencit (Mus musculus L.) Jantan yang Diinduksi Asam Asetat 0,7%

2020 ◽  
Vol 18 (1) ◽  
pp. 68
Author(s):  
ANDREW THEO WILLIAM CHRIS PURMATA ◽  
LESTARI DEWI ◽  
CHOESNAN EFFENDI
Keyword(s):  
Acetic Acid ◽  
Traditional Medicine ◽  
Mus Musculus ◽  
Analgesic Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Mangrove Species ◽  
Avicennia Alba

<p><strong>Background </strong>: <em>Avicennia alba </em>or known as mangrove api-api putih<em> </em>is a type of mangrove species which has many benefits and often to be used by the local Indonesians as traditional medicine. The alcaloid and flavonoid substances derived from <em>Avicennia alba </em>leaves<em> </em>could inhibit pain. This study aims to determine the analgesic effect of <em>Avicennia alba </em>leaves  extract in male mice (<em>Mus musculus L.</em>) induced by acetic acid 0,7%.<strong></strong></p><p class="Default"><strong>Method </strong>:25 mice were divided into 5 groups that were given different therapies. The therapy are 10 mL/kg bw of water, 150 mg/kg bw of acetosal, 250 mg/kg bw; 500mg/kg bw; 1000 mg/kg bw of <em>Avicennia alba </em>leaves extract. 0,7% acetic acid with dose of  10 mL/kg bw is given to each subject intraperitoneally 30 minutes after the therapy to induce pain. The pain is manifested as writhes in mice, the writhes then are counted repeatedly each 10 minutes for 30 minutes.</p><p class="Default"><strong>Result</strong>:<strong>:  </strong>mean of writhes in group treated with <em>Avicennia alba </em>leaves extract in 250 mg/kg bw and 500 mg/kg bw with each value 6,20 and 9,40 are less than mean of writhes in negative control group with value 31,40. %inhibition of <em>Avicennia alba </em>leaves extract with dose of 250 mg/kg bw and 500 mg/kg bw respectively are 80,25% and 70,06%.</p><p><strong>Conclusion </strong>: <em>Avicennia alba </em>leaves extract in 250 mg/kg bw and 500 mg/kg bw dosage showed analgesic effect in male mice (<em>Mus musculus L</em>.) induced with 0,7% acetic acid.</p><p><strong>Keywords </strong>:pain, analgesic, <em>Avicennia alba</em></p>

scholarly journals In vivo enzyme activity and induction of DNA damage in Swiss albino male mice by automobile waste leachate

2018 ◽  
Vol 10 (2) ◽  
pp. 285-295
Author(s):  
Abass Toba Anifowoshe ◽  
Oluyinka Ajibola Iyiola ◽  
Temitope Fatima Olafimihan ◽  
Segun Olayinka Oladipo ◽  
Sunday Frank Yakubu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Positive Control ◽  
Negative Control ◽  
Motor Vehicles ◽  
Genetic Constitution ◽  
Male Mice ◽  
Serum Enzyme ◽  
Sperm Abnormality ◽  
Waste Leachate

The rapid growth of motor vehicles use, together with poor waste disposal, produce environmental and biological threats. We evaluated the genotoxicity and enzyme activity of simulated automobile waste leachate in Swiss albino male mice (Mus musculus). Four mice per group were intraperitoneally treated with four leachate concentrations (10%, 15%, 20% and 25% v/v: simulant (IOASL)/distilled water), as well as a negative control (0.5mL UILSL); and a positive control (cyclophosphamide 20mg/Kg body weight) for five consecutive days. There was a concentration-dependent increase in sperm abnormality compared to the negative control (except at 10% and 15%; p<0,05). Heavy metal (Pb, Cd, As, Hg, Cr, Cu, Fe and Zn) exceeded permissible limits for waste water. Significant variability was also recorded in liver serum enzyme activity (AST, ALP, ALT and ALB) and in the frequencies of micronuclei (p<0,05). The interaction of some of these components with the genetic constitution of the cell during spermatogenesis might be responsible for the abnormalities. 

scholarly journals Reduction of Salmonella in Pork Trimmings

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
H. C. Cobb ◽  
D. Sajeev ◽  
H. T. Thames ◽  
S. Park ◽  
S. B. White ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Treatment Time ◽  
Imaging System ◽  
Acetic Acid Solution ◽  
Positive Control ◽  
Negative Control ◽  
Time Interaction ◽  
Serovar Typhimurium ◽  
In Vivo Bioluminescence Imaging

ObjectivesThe objective of the current study was to determine the efficacy of dipping pork trimmings in acetic acid on Salmonella reduction.Materials and MethodsPork loins were purchased from a commercial purveyor and trimmed of external fat and connective tissues, leaving only the longissimus muscle, which was further cut into 2.5 cm (W) × 2.5 cm (L) × 1.3 cm (H) cubes. Pork cubes were randomly assigned to a negative control (no inoculation, no dipping; NEG), a positive control (inoculation, no dipping; POS), acetic acid dipping at 21°C (ACC) and acetic acid dipping at 50°C (ACH) with a 15-, 45-, or 75-s dipping duration (n = 10 per treatment × time combination). Two inoculation levels, 108 Colony Forming Unit (CFU)/cube of bioluminescent gene-modified (Lux) or 105 CFU/cube of nalidixic acid-resistant Salmonella enteritica serovar Typhimurium, were inoculated onto pork cubes to determine the antibacterial effects of each treatment condition by in vivo bioluminescence imaging system (IVIS) or direct CFU measurement on XLD agar, respectively. In Experiment 1, the cubes were dipped for 15 s to measure the reduction effects by employing both IVIS and CFU. In Experiment 2, cubes were dipped with three dipping durations and the CFU were calculated. The common logarithm of Lux and CFU were calculated and analyzed by the GLIMMIX procedure of SAS v9.4 (SAS Institute Inc., Cary, NC). Actual probability values were reported.ResultsIn Experiment 1, at 108 inoculation level, ACC and ACH reduced the growth of Salmonella by 1.8 and 1.6 log, respectively (P < 0.001) without treatment difference (P = 0.207). However, at 105 inoculation level, ACC and ACH reduced Salmonella by 0.2 and 0.3 log, respectively (P ≤ 0.026). In Experiment 2, at 105 inoculation level with three dipping durations, the ACH treatment reduced Salmonella by 0.9 log more than the ACC treatment (P < 0.001). The 75-s dipping duration was the most effective, providing a reduction of 0.7-log more than the 15-s duration (P = 0.001). No 2-way treatment × time interaction was observed (P = 0.104).ConclusionThe present study suggests that the pork trimmings be dipped into 3% acetic acid solution at 50°C for at least 75 s to ensure the safety of further processed pork products.

scholarly journals Efek Analgetik Ekstrak Etanol Kelopak Rosella (Hibiscus sabdariffa L) Pada Mencit Jantan (Mus musculus)

2017 ◽  
Vol 8 (2) ◽  
pp. 64
Author(s):  
Ridwan Baihaqi ◽  
M. Yulis Hamidy ◽  
Eka Bebasari
Keyword(s):  
Acetic Acid ◽  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Prostaglandin Synthesis ◽  
Hibiscus Sabdariffa ◽  
Antipyretic Effect ◽  
Treatment Groups ◽  
Post Test

Indonesia has been known for its traditional medicine and one of the plants used for tradisional medicine is Hibiscussabdariffa L. The roselle (Hibiscus sabdariffa L) calyx has antipyretic effect by blocking prostaglandin synthesis. Thepurpose of this study was to find out the existences of analgesic effect of roselle (Hibiscus sabdariffa L) calyx ethanolextract. This experimental research used a post test only design that was conducted on 25 male mices weighting 20-35 g. The mices were divided into 5 treatment groups and given the CMC-Na 0,5% (negative control), asetosal 65 mg/kgBB (positive control) and groups of roselle (Hibiscus sabdariffa L) calyx ethanol extract dose of 200 mg/kgBB,400 mg/kgBB and 800 mg/kgBB. Pain stimulus was given chemically by using 1% acetic acid that injectedintraperitoneally 30 minutes after sample was given the extract, the response of the mices will be writhing. It calledpositive response if percentage potency of analgesic e” 50%. The result of this research showed that all dosage ofroselle (Hibiscus sabdariffa L) calyx ethanol extract had an analgesic effect.

scholarly journals In Vivo Histological Study the Potential of Borago officinalis on Spleen and Testis Histo-Architecture on CCL4 Induced Albino Male Mice

2021 ◽  
Vol 8 (6) ◽  
pp. 79-83
Author(s):  
Marwa A. Kubba
Keyword(s):  
Plant Extracts ◽  
Histological Study ◽  
Folk Medicine ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Borago Officinalis ◽  
Ccl4 Treatment ◽  
History Of

History of medicine and plants dates backside to seclude past when herbal treatment was used and be the only answer to all kind of pain and disease. Nowadays, greater prominence is again to use phytotherapy all over the world. Herbal medicine is a traditional or folk medicine that based on the use of plants’ seeds, berries, roots, leaves, barks, flowers and plant extracts for medicinal purposes. This research study focused the line on the potentail of aqueous and methanolic extract of Borago officinalis (Borago; BO) on spleen and testis of albino male mice alone or after interaction between both plant extracts with CCL4 (toxic compound) in comparison to controls group (negative control; without any treatment and positive control; mice treated with CCL4 only). The results indicated the ability of plant extracts to modulate toxic effect resulted from CCL4 treatment.

scholarly journals In-vivo Antipyretic Effect of Eel (Anguilla bicolor bicolor) Oil on Yeast-induced Fever on Mice

2019 ◽  
Vol 15 (2) ◽  
pp. 219
Author(s):  
Heru Sasongko ◽  
Aulia Ayu Rahmawati ◽  
Yeni Farida ◽  
Sugiyarto Sugiyarto
Keyword(s):  
Body Weight ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Omega 3 ◽  
Antipyretic Effect ◽  
Antipyretic Activity ◽  
Epa And Dha

<p>Fish oil has been studied for medicinal purposes, including its antipyretic properties. Eel (<em>Anguilla bicolor bicolor</em>) oil, which contains vitamins and fatty acids, including Omega-3 (EPA and DHA), is also expected to have the antipyretic effect. This research aimed to examine the antipyretic activity of eel oil on white mice (<em>Mus musculus</em> L.). An in-vivo study was done on thirty Swiss-Webster strain males mice that previously got 20% yeast-induced fever. Six treatments were applied including normal group (untreated), a negative control group (yeast-treated), a positive control group treated with acetaminophen (1.764 mg/20 g body weight), and three groups treated with eel oil (0.048, 0.096 and 0.192 g/20 g body weight, respectively). The data was analyzed statistically using one way ANOVA then was continued with LSD post hoc test. The results showed that eel oil has significantly reduced yeast-induced hyperthermia on mice five hours after application at doses 0.096 and 0.192 g/20 g body weight. Our finding suggests that eel oil possess antipyretic properties when was applied in certain doses, and this effect is presumably attributed to its high content of fatty acid, including EPA and DHA.</p>

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

Sign in / Sign up

Export Citation Format

Share Document