Incidence of Microorganisms on Environmental Surfaces in Some Secondary Schools in Birnin Kebbi, Kebbi State, Nigeria

2019 ◽  
pp. 1-14
Author(s):  
Famubo, Joseph A. ◽  
Oladunjoye, Bunmi B. ◽  
Sofoluwe, Yetunde O.
Keyword(s):  
Secondary School ◽  
Secondary Schools ◽  
Representative Sample ◽  
School Setting ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Microbiological Analysis ◽  
Mannitol Salt Agar ◽  
Environmental Surfaces ◽  
Bacteria And Fungi

Due to the ubiquitous nature of bacteria and fungi, the microbiological analysis of environmental surfaces (chairs, tables, floors, hand rails, toilet door knobs and class door knobs) of five (5) secondary schools in Birnin Kebbi metropolis was carried out. Surface swabbing method was used for the collection of a representative sample on the surfaces. Media such as Nutrient agar, Eosin methylene blue, Sabouraud dextrose agar, and Mannitol salt agar were used for the isolation of the organisms. The total bacterial count ranged from 1.1×102 to 9.9×103 CFU/ml, while the total fungi count ranged from 0.0 to 3.6 ×103 CFU/ml. The bacteria isolated include: Corynebacterium kutsceri, Lactobacillus casei, Bacillus sphaerious, Staphlococcus aureus, Bacillus subtilis, Aeromonas spp, staphylococcus epidermidis, Pseudomonas spp, Micococcus varians, while the fungi isolated include Rhizopus stolonifer, Saccharyomyces cerevisae, Alternaria alternate, Aspergillus niger, Mucor spp., Fusarium spp. The isolation of these organisms followed series of procedures, starting with samples collected with swab sticks emulsified with peptone water, followed by the sterile dilution of each sample to a factor of 10-1 for chairs, tables, hand rail and door knobs while floor was diluted to a factor of 10-2. One millilitre (1ml) aliquots of which was used as a representative sample used for isolation of pure colonies was followed by series of biochemical test to confirm the identification of each isolate. Despite the routine cleaning practiced in secondary school setting, isolation of microorganisms from secondary school setting is inevitable.

scholarly journals Impact of a disinfecting technique on microbial contamination of computer keyboards and telephone handsets

2013 ◽  
Vol 2 (4) ◽  
pp. 1 ◽  
Author(s):  
Gabriele Messina ◽  
Emma Ceriale ◽  
Sandra Burgassi ◽  
Carmela Russo ◽  
Chiara Defranceschi ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Local Health ◽  
Signed Rank ◽  
Colony Forming Units ◽  
Before And After ◽  
Health Care Associated Infections ◽  
Elastic Compound ◽  
Bacteria And Fungi

Background: Computers and telephones are ubiquitous in the hospital and have been shown to be contaminated with potentially pathogenic microorganism. The aim of the study was to determine microbial contamination on computer keyboards and telephone handsets and the effectiveness of a disinfecting technique (DT). Methods: A matched cross-over study, involving an overall of 50 computer keyboards and 50 telephone handsets, was conducted in three hospitals, located in the Local Health Area of Siena (Italy) before and after the use of an innovative DT consisting of a malleable-elastic compound, containing ethanol, which adheres to surfaces, removes dirt and disinfects. Total bacterial count was evaluated and several types of bacteria and fungi were researched pre- and post- use of the DT. Non parametric tests: Wilcoxon signed rank and Mann Whitney, were used, when appropriate. Results: The DT was effective in disinfecting the objects. In fact, Colony-Forming Units (CFUs) decreased to zero in most comparisons. All the comparisons showed significant differences (p<0.001) after the DT, both for computer keyboards and telephone handsets. The only exception was found for molds in telephones (p=0.062). Keyboards emerged to be dirtier than telephones handsets (p<0.05) for several types of bacteria. Conclusions: The data suggest that microbial contamination of keyboards and telephones is considerable. These objects can be a vehicle for Health Care-Associated Infections HAIs and their disinfection should not be neglected. The DT showed to be appropriate for the disinfecting purpose.

scholarly journals The Causes of a Lack of Discipline among Secondary School Learners in Mauritius

2018 ◽  
Vol 9 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Belle Louis Jinot
Keyword(s):  
Secondary School ◽  
Secondary Schools ◽  
Peer Group ◽  
School Setting ◽  
Ministry Of Education ◽  
Working Parents ◽  
Dysfunctional Family ◽  
Face To Face ◽  
Group Interviews ◽  
Set Up

AbstractA lack of learners’ discipline is a major school problem in secondary schools in Mauritius. The study aims at determining and examining the main causes of this problem in the context of Mauritius. Qualitative data were collected from learners, educators, principals and parents of four secondary schools by using focus group interviews and individual face-to-face interviews. By using content analysis, the study revealed that the causes of learners’ lack of discipline originate from the family (the parenting style, working parents, ineffective parental discipline and the dysfunctional family), the learners’ attitudes to education and schooling, the educators’ attitudes to their role of maintaining learner discipline, the principals’ lack of authority and leadership in managing learner discipline and the influence of peer group in the school setting. The study shows that all the stakeholders of the school community are responsible for the deterioration of learner discipline in secondary schools. It recommends that there should be a decentralisation of learner discipline strategies from the Ministry of Education to the secondary school principals who should be empowered to set up their institutional school discipline plan.

scholarly journals Effect of fortification of skim milk with coconut milk on the proximate composition and manufacture of dahi, a traditional sweet curd

2016 ◽  
Vol 2 (2) ◽  
pp. 247-252
Author(s):  
Md Rezaul Hai Rakib ◽  
Morsheda Yesmin ◽  
Md Abu Hemayet ◽  
Md Ahsanul Kabir ◽  
Md Nurul Islam
Keyword(s):  
Nutritional Value ◽  
Skim Milk ◽  
Bacterial Count ◽  
Coconut Milk ◽  
Partial Replacement ◽  
Total Bacterial Count ◽  
Microbiological Analysis ◽  
Alternative Source ◽  
Organoleptic Score ◽  
Different Levels

The experiment was conducted to measure the feasibility of partial replacement of skim milk with different levels of coconut milk in the manufacture of dahi. Skimmed milk was replaced by 0, 5, 10, 15 and 20% of coconut milk to produce Dahi designated as A, B, C, D and E. All the samples were analyzed for organoleptic, chemical and microbiological qualities. Replacement of skim milk up to 10% with coconut milk increased total organoleptic score but score decreased when level of coconut milk was 15% and 20%. Dahi manufactured by incorporating 5% coconut milk gave superior results for body and consistency and also for colour and texture. In all levels of replacement increased fat, carbohydrates, ash and total solids were obtained, while protein and pH content were significantly decreased in Dahi samples. Total bacterial count was higher in the dahi manufactured by replacement of coconut milk than control. It could be concluded that 5 and 10% replacement of skim milk with coconut milk for the manufacturing of Dahi was acceptable and reduced the production cost and they were better in compare to others, according to organoleptic, chemical and microbiological analysis. The work showed the potential of coconut as an alternative source of skim milk in dahi manufacturing with improved nutritional value and consumer acceptability.Asian J. Med. Biol. Res. June 2016, 2(2): 247-252

A Study to Evaluate the Efficacy of an 810-nm Diode Laser in the Maintenance of Dental Implants: A Peri-Implant Sulcular Fluid Analysis

2020 ◽  
Vol 46 (4) ◽  
pp. 381-388
Author(s):  
Prerna Sanjay Ghodke ◽  
Rashmi Hegde ◽  
Waqas Ansari ◽  
Sangeeta Muglikar ◽  
Alia S. Dholkawala
Keyword(s):  
Real Time ◽  
Dental Implants ◽  
Diode Laser ◽  
Bacterial Count ◽  
Bactericidal Effect ◽  
Total Bacterial Count ◽  
Microbial Count ◽  
Microbiological Analysis ◽  
Median Percentage ◽  
Fluid Analysis

Biological implant failures are primarily related to biofilm, which can lead to peri-mucositis and, further on, peri-implantitis. The 810-nm diode laser has an affinity for pigmented chromophores, so its use in the peri-implant sulcus has a significant bactericidal effect on the black-pigmented anaerobes such as Porphyromonas gingivalis. Therefore, it can be used to eliminate or reduce the bacterial count in the peri-implant sulcular fluid (PISF), thus increasing the life of the implants and reducing the chances of failure. The purpose of this study was to evaluate the efficacy of the 810-nm diode laser for the maintenance of dental implants and its use as a regular in-office tool for limiting the microbiological count in the PISF. Twenty patients undergoing implant treatment at the Department of Periodontology and Oral Implantology were randomly selected for the study. PISF samples were collected before and after the sulcus was lased with an 810-nm diode laser and sent for quantitative microbiological analysis using universal bacterial count, and the quantity of P gingivalis was evaluated using real-time polymerase chain reaction (PCR). The analysis revealed that after diode application, the median percentage drop in the microbial count was 76.67% and the median percentage drop in P gingivalis count was 99.28%. The use of an 810-nm diode laser resulted in the following outcomes: (1) drastic reduction in the total bacterial count around the implant and (2) significant reduction in the P gingivalis count, as evaluated by real-time PCR.

scholarly journals Preparation of dahi from skim milk with different level of carrot juice

2016 ◽  
Vol 45 (1) ◽  
pp. 36-43 ◽  
Author(s):  
MN Islam ◽  
AAM Muzahid ◽  
R Habib ◽  
MA Mazed ◽  
MA Salam
Keyword(s):  
Chemical Analysis ◽  
Skim Milk ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Microbiological Analysis ◽  
Carrot Juice ◽  
Total Solids ◽  
Physical Chemical ◽  
Different Types ◽  
Microbiological Parameters

An experiment was conducted to measure the feasibility of incorporating carrot (Dascusa carota) juice in the manufacture of dahi. Four different types of dahi were manufactured by adding 0, 5, 10, and 15 per cent carrot juice to skim milk. The prepared dahi samples were subjected to physical, chemical and microbiological analysis to evaluate their qualities. Data on physical, chemical and microbiological parameters were recorded and analyzed. It was observed that the addition of carrot juice with skim milk improved the physical qualities. Dahi with 5% carrot juice was superior to other dahi samples in respect of smell and taste, body and consistency and also for colour and texture. Chemical analysis showed that addition of carrot juice increased the total solids and acidity content but decreased the protein, fat, ash and pH content. Total bacterial count was higher in the dahi samples manufactured by incorporating carrot juice than the control. From this study, it was suggested that dahi could be successfully manufactured from skim milk by incorporating carrot juice and 5% was found better for this purpose.Bang. J. Anim. Sci. 2016. 45 (1): 36-43

scholarly journals Assessment of physicochemical, microbiological, mineral and heavy metal parameters of honey

2021 ◽  
Vol 4 (1) ◽  
pp. 013-018
Author(s):  
Bello Muhammed Magaji ◽  
Bamidele Joshua Awogbemi ◽  
Agnes Yemisi Asagbra ◽  
Fatunmibi Omolara Omowumi ◽  
Femi Maroof Adams
Keyword(s):  
Bacterial Count ◽  
Total Solid ◽  
Reducing Sugar ◽  
National Standards ◽  
Total Bacterial Count ◽  
Microbiological Analysis ◽  
Total Acidity ◽  
Mineral Analysis ◽  
Physico Chemical ◽  
Metallic Contaminants

The qualities of the honey were evaluated using the following attributes Physico-chemical, Microbiological, minerals and metallic contaminants respectively. The Physico-chemical analysis revealed a moisture content of 17.45%, Ash 0.64%, Total solid 81.25%, Fat 0.64%, Protein 0.25%, Fibre 0.03%, Total carbohydrate81.22%, Energy value of 329.5 Kcal, [email protected], Sucrose content 5.48%, Nitrogen content 0.004%, Total acidity 3.45%, Reducing sugar 61.82%, insoluble water content 0.06%, Refractive index of 1.477, and Specific gravity of 1.234 respectively. The microbiological analysis showed a total bacterial count TBC of 7x102 cfu/g, Yeast count 2x101cfu/g and Mould count 2x101cfu/g, which was within the national standards range by SON as 1x102 cfu/g, 5x101 cfu/g, and 5x101 cfu/g respectively while the Coliform count, Escherichia count, Salmonella count, Staphylococus count and Clostridium count were not detected this research. Generally, honey may contain organisms from bees, soil, air and dust that may be introduced during post-harvest handling. This is evidence that honey is well preserved against bacteria so that these organisms would not survive unfavourable conditions. The mineral analysis showed the presences of potassium{K), Calcium (Ca), Sodium (Na), Magnesium (Mg), Zink (Zn), Iron (Fe) Arsenic (As) respectively with values of 70.5, 21.0, 95.0,12.0, 1.3, 1.0, and 1.0 (Mg/100g) while Cupper and Lead were not detected.

scholarly journals BACTERIAL CONTAMINATIONS OF USED FACE MASKS COLLECTED FROM DIFFERENT CLINICAL SECTIONS IN A UNIVERSITY TEACHING HOSPITAL DURING COVID -19 PANDEMIC CRISES IN NIGERIA

2021 ◽  
Vol 4 (1) ◽  
pp. 8-10
Author(s):  
Obhioze Augustine Akpoka ◽  
A. A. Enaigbe ◽  
C. C. Irodi
Keyword(s):  
Teaching Hospital ◽  
Screening Test ◽  
Bacterial Load ◽  
Bacterial Count ◽  
University Teaching ◽  
Nutrient Agar ◽  
Total Bacterial Count ◽  
Frequency Of Occurrence ◽  
Bacterial Isolates ◽  
Bacteria And Fungi

This study was carried out to identify and characterize the bacteria and fungi present in used face masks, obtained from some clinical sections of University Teaching Hospital in Nigeria. The phenotypic; microbiological and biochemical identifications of the microorganisms were determined by the pour plate techniques using, nutrient agar according to established standard protocols. The results recorded a mean total bacterial count that ranged from 1.75 ± 0.12 log10 CFU/ml to 4.36 ± 0.28 log10 CFU/ml. However, the samples collected from the Anatomy section recorded the lowest bacterial count (1.75 ± 0.12 log10 CFU/ml) while samples obtained from the Nursing section had the highest bacterial load (4.36 ± 0.28 log10CFU/ml). The overall screening test showed the presence of three bacterial isolates: Peptococcus, Pseudomonas and Staphylococcus; Consequently, from the total 64 counted bacterial colonies, the frequency of occurrence was predominated by the Pseudomonas 40 (62.5 %) while, mild counts were respectively reported for Staphylococcus and Peptococcus 18 (28.1 %) and 06 (9.40 %).  Succinctly, this work has highlighted that, the underlying illnesses such as lung abscesses, asthma, otitis, pneumonia and sinusitis associated with the isolated organisms in this experiment are also directly linked or similar to the symptoms displayed in mild and severe cases of COVID-19 patients.

scholarly journals Influence of Ozone on the Biochemical Composition of Birch Sap

2021 ◽  
Vol 11 (7) ◽  
pp. 2965
Author(s):  
Simona Paulikienė ◽  
Justas Mingaila ◽  
Vladas Vilimas ◽  
Edmundas Bartkevičius ◽  
Pranas Viskelis ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Biochemical Composition ◽  
Statistical Significance ◽  
Titratable Acidity ◽  
Bacterial Count ◽  
Ozone Treatment ◽  
Total Bacterial Count ◽  
Microbiological Analysis ◽  
Total Sugars ◽  
Number Of Microorganisms

Studies have shown that ozone is a good oxidizer and a strong disinfectant. There are many uses for ozone in the food industry, but there is relatively little information about the influence of ozone on biochemical composition and the capacity to reduce the number of microorganisms in birch sap. In this study, sap was ozonated at different intervals for 5 min (O3: 0.087 ± 0.009 mg L−1), 10 min, 15 min, 20 min, 25 min, or 30 min (O3: 0.99 ± 0.09 mg L−1). The parameters of the birch sap were studied immediately after the ozone treatment as well as during storage for seven days at 2 °C and for five days at 20 °C. The parameters of ozonated birch sap were compared with the parameters of fresh sap (control). The microbiological analysis included total bacterial count, lactic acid bacterial count, and yeast and mold count. Birch sap color, pH, titratable acidity, and ºBrix values were also determined. Evaluation of monosaccharides, sucrose, total sugars, and ascorbic acid was carried out in fresh sap as well as sap ozonated for 30 min, immediately after ozonation. The results show the statistical significance of the inactivation of microorganisms after treatment in most cases. The microorganism counts gradually reduced with increasing intervals of ozone treatment. The best results were obtained after 25 and 30 min of ozonation. Ozone treatment did not significantly influence the pH, titratable acidity, or °Brix statistically. Values of monosaccharides, sucrose, total sugars, and ascorbic acid were influenced within the margin of error. Ozone had a significant influence on the chroma and hue angle.

The use of Self-Recording in Behaviour Modification in a Secondary School

1979 ◽  
Vol 7 (3) ◽  
pp. 57-66 ◽  
Author(s):  
E. McNamara
Keyword(s):  
Secondary School ◽  
Secondary Schools ◽  
Primary Schools ◽  
School Setting ◽  
Successful Implementation ◽  
School Teachers ◽  
Primary School Teachers ◽  
Behaviour Modification ◽  
Nursery Schools ◽  
Service Training

While behaviour modification techniques are becoming more widely used in primary schools (Ward, 1971; Harrop, 1974; Presland, 1978), special education (McNamara, 1977; Ward, 1975) and even nursery schools (Coleman and Laishley, 1977), only a few studies have demonstrated successful implementation in Secondary Schools (McAllister et al, 1969; Broden et al, 1971). Harrop (1977) found that his in-service training course which had been successful with Primary School teachers was less successful in Secondary Schools. Lane (1977) argues that this is partly due to an inadequate initial functional analysis, whilst McNamara (1977) lists a number of reasons why implementation may be more difficult in the Secondary School setting.

scholarly journals Athletic Trainer Services in US Private Secondary Schools

2016 ◽  
Vol 51 (9) ◽  
pp. 717-726 ◽  
Author(s):  
Alicia Pike ◽  
Riana R. Pryor ◽  
Stephanie M. Mazerolle ◽  
Rebecca L. Stearns ◽  
Douglas J. Casa
Keyword(s):  
United States ◽  
Secondary School ◽  
Secondary Schools ◽  
School Administrators ◽  
School Setting ◽  
The United States ◽  
Athletic Trainer ◽  
Full Time ◽  
The Public

Context: Availability of athletic trainer (AT) services in US secondary schools has recently been reported to be as high as 70%, but this only describes the public sector. The extent of AT coverage in private secondary school settings has yet to be investigated and may differ from the public secondary school setting for several reasons, including differences in funding sources. Objective: To determine the level of AT services in US private secondary schools and identify the reasons why some schools did not employ ATs. Design: Concurrent mixed-methods study. Setting: Private secondary schools in the United States. Patients or Other Participants: Of 5414 private secondary schools, 2044 (38%) responded to the survey. Main Outcome Measure(s): School administrators responded to the survey via telephone or e-mail. This instrument was previously used in a study examining AT services among public secondary schools. Descriptive statistics provided national data. Open-ended questions were evaluated through content analysis. Results: Of the 2044 schools that responded, 58% (1176/2044) offered AT services, including 28% (574/2040) full time, 25% (501/2042) part time, 4% (78/1918) per diem, and 20% (409/2042) from a hospital or clinic. A total of 84% (281 285/336 165) of athletes had access to AT services. Larger private secondary schools were more likely to have AT services available. Barriers to providing AT services in the private sector were budgetary constraints, school size and sports, and lack of awareness of the role of an AT. Conclusions: More than half of the surveyed private secondary schools in the United States had AT services available; however, only 28% had a full-time AT. This demonstrates the need for increased medical coverage to provide athletes in this setting the appropriate level of care. Budgetary concerns, size of the school and sport offerings, and lack of awareness of the role of the AT continued to be barriers in the secondary school setting.

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