Topographic Analysis of Intertidal Polychaete Reefs (Sabellaria alveolata) at a Very High Spatial Resolution

In temperate coastal regions of Western Europe, the polychaete Sabellaria alveolata (Linné) builds large intertidal reefs of several hectares on soft-bottom substrates. These reefs are protected by the European Habitat Directive EEC/92/43 under the status of biogenic structures hosting a high biodiversity and providing ecological functions such as protection against coastal erosion. As an alternative to time-consuming field campaigns, a UAV-based Structure-from-Motion photogrammetric survey was carried out in October 2020 over Noirmoutier Island (France) where the second-largest known European reef is located in a tidal delta. A DJI Phantom 4 Multispectral UAV provided a topographic dataset at very high resolutions of 5 cm/pixel for the Digital Surface Model (DSM) and 2.63 cm/pixel for the multispectral orthomosaic images. The reef footprint was mapped using a combination of two topographic indices: the Topographic Openness Index and the Topographic Position Index. The reef structures covered an area of 8.15 ha, with 89% corresponding to the main reef composed of connected and continuous biogenic structures, 7.6% of large isolated structures (<60 m2), and 4.4% of small isolated reef clumps (<2 m2). To further describe the topographic complexity of the reef, the Geomorphon landform classification was used. The spatial distribution of tabular platforms considered as a healthy stage of the reef in contrast to a degraded stage was mapped with a proxy that consists in comparing the reef volume to a theoretical tabular-shaped reef volume. Epibionts colonizing the reef (macroalgae, mussels, and oysters) were also mapped by combining multispectral indices such as the Normalised Difference Vegetation Index and simple band ratios with topographic indices. A confusion matrix showed that macroalgae and mussels were satisfactorily identified but that oysters could not be detected by an automated procedure due to their spectral complexity. The topographic indices used in this work should now be further exploited to propose a health index for these large intertidal reefs.

INVERTEBRATES OF CONSERVATIVE INTEREST FROM PLATFORMA COTMEANA, A ROMANIAN NATURAL PROTECTED AREA

Current work was carried out in a Natura 2000 site of community importance, ROSCI 0354, Platforma Cotmeana, with a surface of 12.529 ha, located in the Central Southern part of Romania in a hilly area. The site is mainly dominated by broadleaf forest, from which 30% consists of beech species, an important habitat component for the studied species. Focal species of this research were: Lucanus cervus (Linnaeus, 1758), Cerambyx cerdo Linnaeus, 1758, and Morimus asper funereus Mulsant, 1862, as listed in the annex of EU Habitat Directive. For each species, numerical abundance, sex ratio, and other aspects of their biology were estimated. Habitat preference and the occurrence of individuals were recorded in order to evaluate their distribution in the site. Future perspectives on the status of the conservation of species in site were assessed by evaluation of human impact activities affecting the quality of their habitat. It was noted that most dominant species is L. cervus with an occurrence of 314 individuals, followed by M. asper funereus with 92 individuals, and C. cerdo with 41 individuals. An exoskeleton of Rosalia alpina (Linnaeus, 1758) was found, fact explained by the limit of species distribution area in the site.

THE POTENTIAL OF SENTINEL-1 DATA TO SUPPLEMENT HIGH RESOLUTION EARTH OBSERVATION DATA FOR MONITORING GREEN AREAS IN CITIES

Green areas play an important role within urban agglomerations due to their impact on local climate and their recreation function. For detailed monitoring, frameworks like the flora fauna habitat (FFH) classification scheme of the European Union’s Habitat Directive are broadly used. By date, FFH classifications are mostly expert-based. Within this study, a data-driven approach for FFH classification is tested. For two test areas in the municipality of Vienna, ALS point cloud data are used to derive predictor variables like terrain features, vegetation structure and potential insulation as well as reflection properties from full waveform analysis on a 1 m grid. In addition, Sentinel-1 C-Band time series data are used to increase the temporal resolution of the predicting features and to add phenological characteristics. For two 1.3 × 1.3 km test tiles, random forest classifiers are trained using different combinations (ALS, SAR, ALS+SAR) of input features. For all model test runs, the combination of ALS and SAR input features lead to best prediction accuracies when applied on test data.

An unknown hotspot of plant diversity in the heart of the Central Apennine: flora and vegetation outline of Mt. Pozzoni-St. Rufo valley (Cittareale, Rieti)

Surprisingly enough, Italy still has some botanically unexplored areas; among these there are some territories between Lazio, Umbria and Abruzzo not included in any protected area. The study area, ranging for 340 ha, includes the mountainous area of Mt. Pozzoni-Mt. Prato-St. Rufo valley, which forms the upper part of the river Velino basin, located in the territory of the municipality of Cittareale (Rieti, Lazio), at an elevation from 1150 to 1903 m a.s.l. The substrate is mainly made of marly limestone of the Meso-Cenozoic Umbria-Marche sedimentary succession. The climate is Temperate and comprises vegetation belts from the montane to sub-alpine. Land cover is dominated by pastures and deciduous forests, with only a few hay meadows. 794 entities have been detected: 16% are considered rare or very rare for the regional territory with several floristic novelties for the regional flora, 6% of the total was found to be endemic to Italy and only eight taxa were aliens. Four taxa are new for the regional flora of Lazio: Arum cylindraceum, Alopecurus pratensis subsp. pratensis, Hieracium bupleuroides and Trinia glauca subsp. glauca. Forest vegetation is represented by beech forests, while dry grasslands are the most widespread vegetation type. The greatest phytocoenotic diversity was found within the secondary pastures. Particularly interesting is the plant community with Iris marsica, which suggests that limestone mountain ledges can represent a primary habitat for this endemic species of the Central Apennine. The presence of several habitats listed in the EU Habitat Directive indicates how the lack of detailed territorial knowledge can lead to the non-designation of conservation sites in areas of high naturalistic value. These findings showed that botanical explorations in territories which are still not known could contribute significantly to the identification of areas of high interest in conserving plant diversity.

From Remote Sensing to Species Distribution Modelling: An Integrated Workflow to Monitor Spreading Species in Key Grassland Habitats

Remote sensing (RS) has been widely adopted as a tool to investigate several biotic and abiotic factors, directly and indirectly, related to biodiversity conservation. European grasslands are one of the most biodiverse habitats in Europe. Most of these habitats are subject to priority conservation measure, and several human-induced processes threaten them. The broad expansions of few dominant species are usually reported as drivers of biodiversity loss. In this context, using Sentinel-2 (S2) images, we investigate the distribution of one of the most spreading species in the Central Apennine: Brachypodium genuense. We performed a binary Random Forest (RF) classification of B. genuense using RS images and field-sampled presence/absence data. Then, we integrate the occurrences obtained from RS classification into species distribution models to identify the topographic drivers of B. genuense distribution in the study area. Lastly, the impact of B. genuense distribution in the Natura 2000 (N2k) habitats (Annex I of the European Habitat Directive) was assessed by overlay analysis. The RF classification process detected cover of B. genuense with an overall accuracy of 94.79%. The topographic species distribution model shows that the most relevant topographic variables that influence the distribution of B. genuense are slope, elevation, solar radiation, and topographic wet index (TWI) in order of importance. The overlay analysis shows that 74.04% of the B. genuense identified in the study area falls on the semi-natural dry grasslands. The study highlights the RS classification and the topographic species distribution model’s importance as an integrated workflow for mapping a broad-expansion species such as B. genuense. The coupled techniques presented in this work should apply to other plant communities with remotely recognizable characteristics for more effective management of N2k habitats.

CHECKLIST OF MACRO-INVERTEBRATES OF THE SPECIAL CONSERVA-TION AREA “POGGI DI PRATA” (GROSSETO, CENTRAL ITALY)THROUGH A CITIZEN-SCIENCE AND EXPERT-BASED APPROACH

The assessment of species composition in a certain area may become outdated over time due to community dynamics including species range expansion, but also to local extinctions, species introductions and taxonomic redefinition. Therefore, updated checklists are required for animal conservation and management. Exhaustive checklists of invertebrate species may be challenging, as species determination often requires the analysis by specialists, but they are fundamental for local conservation practices. In this work, we provided an annotated preliminary checklist of invertebrates of the Special Conservation Area “Poggi di Prata” (province of Grosseto, southern Tuscany), detected through field samplings with experts, and a permanent Bioblitz set out on an online citizen-science platform (iNaturalist.org). The final dataset(1898-2020)included 329records of 282 species (217insects, 34gastropods, 30arachnids and 1 chilopod). Most records were uploaded on iNaturalist (about 56.5%), others came from observations or sampling collections(37%) and were determined by specialists. Only the remaining 6.5% of records came from published studies. Three species were protected by the Habitat Directive, 15 by the Tuscan Regional Law. We also detected two endemic or near-endemic taxa of this area: the beetle Paramaurops diecki massetanus and the land snail Marmorana saxetana. The unexpected (Italian southernmost) record of Gaurotes virginea needs to be deepened. Furthermore, 12 alien species, including insects affecting human economy and wellness (e.g., Rhinchophorous ferrugineus, Aedes albopictus, Halyomorpha halys, Dryocosmus kuriphilus and Cydalima perspectalis), were also detected. With our work, we confirmed that citizen-science platforms (e.g. iNaturalist) are valuable tools, complementary to field-work by specialists, to map local biodiversity and they may help to improve biogeographical knowledge.

The role of European rare habitats in conservation of rare phytodiversity of «Drevlianskyi» Nature Reserve

Classification scheme of rare habitats of Europe according to Bern Convention and Habitat Directive of EU was presented for the territory of Drevlianskyi nature reserve. It was shown significant habitat’s diversity of nature reserve from all main types of habitats — water (permanent and temporary lakes (waterbodies), watercources, reedbeds habitats), bog (eutrophic and mesotrophic mires), grassland (dry, mesic and wet grassland, floodplain and fen scrub, heaths), forest (leaved and conifer forests (woodland), rock (rocks and talus of silicate rocks). According to the results of field research, the territory of the reserve represented by 30 habitats (1 — ІІ leaves, 4 — ІІІ leaves, 25 — ІV leaves). It is established that the most common habitat 91T0 (Central European lichen Scots pine forests), which represented by 153 localities in A1C (dry pine forest) on an area of 421.5 ha. It was made a conclusion that the role of rare habitats in nature reserve Drevlianskyi is determinant for conservation of rare species of vascular plants that are protected by the Bern Convention, European Red List and included to the Red Book of Ukraine. The results of the analysis of the role of rare biotopes of Europe in the conservation of species of flora of different protection status on the territory of Drevlianskyi nature reserve show that most of the rare plant species of reserve listed in Resolution № 6 of the Bern Convention (Annex I), revised in 2011, are present in its rare settlements. The role of rare habitats of Drevlianskyi nature reserve in the conservation of rare species of flora is decisive — of the 29 species of plants of supranational and national levels of protection in rare habitats there are 24 species or 82.8% of their total number.

Ecosystem-based approach towards the sustainable management of coastal engineering: compensation and mitigation measures applied to the Civitavecchia harbour

&lt;p&gt;The expansion of Civitavecchia Hub Port will potentially produce an impact on the adjacent coastal areas which are characterized by the presence of SCIs (Sites of Community Importance IT6000005 northern and IT6000006 southern to the port area) which extend in front of the municipalities of Tarquinia, Civitavecchia&amp;#160;and Santa Marinella.&lt;br&gt;The two SCIs are characterized by priority habitats and species under the Habitat Directive 92/43 / EEC; in particular, they host Posidonia oceanica meadows (Priority habitat 1120 * - Posidonion oceanicae) and coralligenous bioconstructions (Habitat 1170 - Reefs), as well as individuals of Pinna nobilis (Annex IV - Habitat Directive Code 1028) and colonies of Corallium rubrum ( Annex IV - Habitat Directive Code 1001).&lt;br&gt;Within VIA and VAS procedures, a series of complex activities has been implemented to reduce the potential impacts on the marine environment, leading to the presentation of an innovative ecosystem-based program of mitigation and compensation measures based on the assessment of direct and indirect impacts of construction activities as well as ecosystem services analysis. This new program is based on our project &quot;Ecosystem-based approach applied to the evaluation of compensation and mitigation measures in marine environment: The case study of the Port Hub of Civitavecchia&quot;. The project includes for example: a detailed analysis of marine habitats and oceanographic conditions, restoration of equivalent habitats, habitats and species protection systems, actions towards an eco-sustainable use of the environment. The innovation of this approach derives from the quantification of the interventions which is based on the assessment of surfaces damage as well as on the uses affected both directly and indirectly, considering a time interval of ten years for the recovery of the damaged ecosystem functions.&lt;/p&gt;

Intra-Annual Sentinel-2 Time-Series Supporting Grassland Habitat Discrimination

The present study aims to discriminate four semi-arid grassland habitats in a Mediterranean Natura 2000 site, Southern Italy, involving 6210/E1.263, 62A0/E1.55, 6220/E1.434 and X/E1.61-E1.C2-E1.C4 (according to Annex I of the European Habitat Directive/EUropean Nature Information System (EUNIS) taxonomies). For this purpose, an intra-annual time-series of 30 Sentinel-2 images, embedding phenology information, were investigated for 2018. The methodology adopted was based on a two-stage workflow employing a Support Vector Machine classifier. In the first stage only four Sentinel-2 multi-season images were analyzed, to provide an updated land cover map from where the grassland layer was extracted. The layer obtained was then used for masking the input features to the second stage. The latter stage discriminated the four grassland habitats by analyzing several input features configurations. These included multiple spectral indices selected from the time-series and the Digital Terrain Model. The results obtained from the different input configurations selected were compared to evaluate if the phenology information from time-series could improve grassland habitats discrimination. The highest F1 values (95.25% and 80.27%) were achieved for 6210/E1.263 and 6220/E1.434, respectively, whereas the results remained stable (97,33%) for 62A0/E1.55 and quite low (75,97%) for X/E1.61-E1.C2-E1.C4. However, since for all the four habitats analyzed no single configuration resulted effective, a Majority Vote algorithm was applied to achieve a reduction in classification uncertainty.

A Mediterranean element of the vegetation: Junco maritimi-Cladietum marisci – a new association for Ukraine

Cladium mariscus (L.) Pohl (Cyperaceae) is a rare species in Europe considered by several authors to be a relict of the early Holocene period. It is listed in the Red Data Book of Ukraine, Annexes of the Habitat Directive and the Bern Convention. Communities with domination of this species are included in the Green Data Book of Ukraine. Substantial differences in major ecological factors for Cladium mariscus communities in the western (carbonate bogs) and the southern (marshes and floating swamps of the northern Black Sea) regions of Ukraine were shown. The author carried out comparisons of relevés characterizing different communities with Cladium mariscus within Europe. Based on the results of TWINSPAN analysis, four associations were identified, confirmed by floristic indices and ecological data: Cladietum marisci Allorge 1921, Soncho maritimi-Cladietum marisci (Br.-Bl. & O. de Bolòs 1957) Cirujano 1980, Dorycnio recti-Cladietum marisci Gradstein & Smittenberg 1977 and Junco maritimi-Cladietum marisci (Br.-Bl. & O. de Bolòs 1957) Géhu & Biondi 1988. Thus, in addition to the association Cladietum marisci, a new one was indicated for Ukraine, Junco maritimi-Cladietum marisci.