Calibration Strategy to Determine the Interaction Properties of Fertilizer Particles Using Two Laboratory Tests and DEM

Investigating the interactions of granular fertilizers with various types of equipment is an essential part of agricultural research. A numerical technique simulating the mechanical behavior of granular assemblies has the advantage of data trackings, such as the trajectories, velocities, and transient forces of the particles at any stage of the test. The interaction parameters were calibrated to simulate responses of granular fertilizers in EDEM, a discrete element method (DEM) software. Without a proper calibration of the interaction parameters between the granular fertilizers and various materials, the simulations may not represent the real behavior of the granular fertilizers. Therefore, in this study, a strategy is presented to identify and select a set of DEM input parameters of granular fertilizers using the central composite design (CCD) to establish the nonlinear relationship between the dynamic macroscopic granular fertilizer properties and the DEM parameters. The determined interaction properties can be used to simulate granular fertilizers in EDEM.

Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes

The spindle generates force to segregate chromosomes at cell division. In mammalian cells, kinetochore-fibers connect chromosomes to the spindle. The dynamic spindle anchors kinetochore-fibers in space and time to move chromosomes. Yet, how it does so remains poorly understood as we lack tools to directly challenge this anchorage. Here, we adapt microneedle manipulation to exert local forces on the spindle with spatiotemporal control. Pulling on kinetochore-fibers reveals the preservation of local architecture in the spindle-center over seconds. Sister, but not neighbor, kinetochore-fibers remain tightly coupled, restricting chromosome stretching. Further, pulled kinetochore-fibers pivot around poles but not chromosomes, retaining their orientation within 3 μm of chromosomes. This local reinforcement has a 20 s lifetime, and requires the microtubule crosslinker PRC1. Together, these observations indicate short-lived, specialized reinforcement in the spindle center. This could help protect chromosome attachments from transient forces while allowing spindle remodeling, and chromosome movements, over longer timescales.

Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes

The spindle generates force to segregate chromosomes at cell division. In mammalian cells, kinetochore-fibers connect chromosomes to the spindle. The dynamic spindle anchors kinetochore-fibers in space and time to coordinate chromosome movement. Yet, how it does so remains poorly understood as we lack tools to directly challenge this anchorage. Here, we adapt microneedle manipulation to exert local forces on the spindle with spatiotemporal control. Pulling on kinetochore-fibers reveals that the spindle retains local architecture in its center on the seconds timescale. Upon pulling, sister, but not neighbor, kinetochore-fibers remain tightly coupled, restricting chromosome stretching. Further, pulled kinetochore-fibers freely pivot around poles but not around chromosomes, retaining their orientation within 3 µm of chromosomes. This local reinforcement has a 20 s lifetime, and requires the microtubule crosslinker PRC1. Together, these observations indicate short-lived, specialized reinforcement of the kinetochore-fiber in the spindle center. This could help the spindle protect local structure near chromosomes from transient forces while allowing its remodeling over longer timescales, thereby supporting robust chromosome attachments and movements.

A Proposed Guideline for Applying Waterhammer Predictions Under Transient Cavitation Conditions: Part 2 — Imbalanced Forces

Waterhammer analysis (herein referred to as Hydraulic Transient Analysis or simply “HTA”) becomes more complicated when transient cavitation occurs (also known as liquid column separation). This complication is exacerbated when trying to predict imbalanced forces as this often involves comparing pressure times area (“PxA”) forces at two locations (for example at elbow pairs). Whereas the pressure at each elbow location has increased uncertainty because of transient cavitation, the difference in PxA forces at elbow pairs involves subtracting one potentially uncertain pressure from another uncertain pressure. Exacerbating this uncertainty yet further, the existence of vapor in a liquid system can dramatically affect the fluid wavespeed and, hence, the timing of the pressure wave travel between two locations such as elbow pairs; so the pressure calculated at each location would not actually occur at exactly the same time. This Part 2 discusses methods of accounting for uncertainty in HTA imbalanced force predictions due to cavitation. The criteria in this paper assume that cavitation in the HTA has been assessed and accepted per the criteria in Part 1 of this paper. A guideline is proposed for accepting and applying such results and, in particular, makes recommendations on safety factors to use in pipe stress analysis for different cases. The specific recommendations depend on numerous factors including: • Presence or absence of cavitation in hydraulically connected or isolated parts of the system • If cavitation occurs, whether the peak forces occur before or after cavitation first occurs • Size of the cavitation vapor volumes with respect to the computing volumes • Use of point forces as a conservative substitute in place of potentially less certain elbow pair forces or the manual assessment of maximum envelope values for the force. Situations are discussed where waterhammer abatement is recommended to reduce hydraulic transient forces, and for increasing confidence in HTA results in specific cases. The result is a proposed comprehensive and pragmatic guideline which practicing engineers can use to perform waterhammer analysis and apply imbalanced force predictions to pipe stress analysis.

Effects of barefoot and minimally shod footwear on effective mass – implications for transient musculoskeletal loading

The purpose of this investigation was to explore the effects of barefoot and minimally shod footwear on effective mass, and determine the implications that this has for transient loading during running. Fifteen male runners ran at 4.0 m/s in five different footwear conditions (barefoot, running trainer, Nike-free, Inov-8 and Vibram five-fingers). Kinematics were collected using an 8 camera motion capture system and ground reaction forces via an embedded force platform. Effective mass was examined using impulse-momentum modelling and differences between footwear were examined using one-way repeated measures ANOVA. The findings showed that effective mass was significantly larger in the barefoot (11.47 %BW), Nike-free (9.81 %BW), Inov-8 (12.10 %BW) and Vibram five-fingers (8.84 %BW) compared to the running trainer (6.86 %BW). Furthermore, instantaneous loading rate was significantly larger in the barefoot (347.55 BW/s), Nike-free (178.76 BW/s), Inov-8 (369.93 BW/s) and Vibram five-fingers (339.37 BW/s) compared to the running trainer (133.18 BW/s). It was also revealed that there were significant positive associations between effective mass and the instantaneous rate of loading for each footwear. The findings from the current investigation indicate that effective mass has key implications for the generation of transient forces and also that running barefoot and in minimally shod footwear may place runners at increased risk from impact related injuries compared to the traditional running shoes

Viscoelastic dissipation stabilizes cell shape changes during tissue morphogenesis

Tissue morphogenesis relies on the production of active cellular forces. Understanding how such forces are mechanically converted into cell shape changes is essential to our understanding of morphogenesis. Here we use Myosin II pulsatile activity during Drosophila embryogenesis to study how transient forces generate irreversible cell shape changes. Analyzing the dynamics of junction shortening and elongation resulting from Myosin II pulses, we find that long pulses yield less reversible deformations, typically a signature of dissipative mechanics. This is consistent with a simple viscoelastic description, which we use to model individual shortening and elongation events. The model predicts that dissipation typically occurs on the minute timescale, a timescale commensurate with that of force generation by Myosin II pulses. We test this estimate by applying time-controlled forces on junctions with optical tweezers. Our results argue that active junctional deformation is stabilized by dissipation. Hence, tissue morphogenesis requires coordination between force generation and dissipation.