Experimental Analysis of Tip Vibrations at Higher Eigenmodes of QPlus Sensors for Atomic Force Microscopy

QPlus sensors are non-contact atomic force microscope probes constructed from a quartz tuning fork and a tungsten wire with an electrochemically etched tip. These probes are self-sensing and offer an atomic-scale spatial resolution. Therefore, qPlus sensors are routinely used to visualize the chemical structure of adsorbed organic molecules via the so-called bond imaging technique. This is achieved by functionalizing the AFM tip with a single CO molecule and exciting the sensor at the first vertical cantilever resonance mode. Recent work using higher-order resonance modes has also resolved the chemical structure of single organic molecules. However, in these experiments, the image contrast can differ significantly from the conventional bond imaging contrast, which was suspected to be caused by unknown vibrations of the tip. This work investigates the source of these artefacts by using a combination of mechanical simulation and laser vibrometry to characterize a range of sensors with different tip wire geometries. The results show that increased tip mass and length cause increased torsional rotation of the tuning fork beam due to the off-center mounting of the tip wire, and increased flexural vibration of the tip. These undesirable motions cause lateral deflection of the probe tip as it approaches the sample, which is rationalized to be the cause of the different image contrast. The results also provide a guide for future probe development to reduce these issues.

Comparison Of Retinopathy And Hearing Impairment In Poorly Controlled Diabetics In Population Of Muzaffarabad AJK

Aim: Prevalence and comparison of retinopathy and sensorineural hearing loss in poorly managed diabetics Methodology: A cross sectional study was designed with the approval of the Ethical Review Board Committee. The study enrolled 50 people who met the inclusion criteria. Anthropometric data were collected using standardized procedures. All laboratory assays were performed on the initial visit to the diabetes research center's laboratory. Tuning fork and PTA examinations of the ears were performed. The scores of Rinne and Weber were recorded and synchronised. Fundoscopy was used in ophthalmic investigations. Results: The Rinne test was found to be normal in 50(100%) cases of population while Weber’s test was centralized in 43(86%) of patients, 1(2%) case was lateralized to right, 5(10%)cases were lateralized to left, 1(2%) case had moderate disease. PTA scores revealed mild disease in 1(2%), moderate 14(28%), severe 1(2%) and 34(68%) patients showed normal results. Fundoscopy demonstrated normal results in 11(22%), Grade 1 retinopathy in 15(30%), grade 2 RP in 17(34%) and grade 3 RP in 7(14%). Conclusion: Retinopathy is more prevalent with poorly managed diabetic individuals in contrast to hearing impairment. Key words: Retinopathy, Fundoscopy, Diabetes mellitus

Reliability of recommended non-invasive chairside screening tests for diabetes-related peripheral neuropathy: a systematic review with meta-analyses

The objective is to determine, by systematic review, the reliability of testing methods for diagnosis of diabetes-related peripheral neuropathy (DPN) as recommended by the most recent guidelines from the International Diabetes Foundation, International Working Group on the Diabetic Foot and American Diabetes Association. Electronic searches of Cochrane Library, EBSCO Megafile Ultimate and EMBASE were performed to May 2021. Articles were included if they reported on the reliability of recommended chairside tests in diabetes cohorts. Quality appraisal was performed using a Quality Appraisal of Reliability Studies checklist and where possible, meta-analyses, with reliability reported as estimated Cohen’s kappa (95% CI). Seventeen studies were eligible for inclusion. Pooled analysis found acceptable inter-rater reliability of vibration perception threshold (VPT) (κ=0.61 (0.50 to 0.73)) and ankle reflex testing (κ=0.60 (0.55 to 0.64)), but weak inter-rater reliability for pinprick (κ=0.45 (0.22 to 0.69)) and 128 Hz tuning fork (κ=0.42 (0.15 to 0.70)), though intra-rater reliability of the 128 Hz tuning fork was moderate (κ=0.54 (0.37 to 0.73)). Inter-rater reliability of the four-site monofilament was acceptable (κ=0.61 (0.45 to 0.77)). These results support the clinical use of VPT, ankle reflexes and four-site monofilament for screening and ongoing monitoring of DPN as recommended by the latest guidelines. The reliability of temperature perception, pinprick, proprioception, three-site monofilament and Ipswich touch test when performed in people with diabetes remains unclear.

Quartz Tuning Fork Sensor-Based Dosimetry for Sensitive Detection of Gamma Radiation

This study generally relates to nuclear sensors and specifically to detecting nuclear and electromagnetic radiation using an ultrasensitive quartz tuning fork (QTF) sensor. We aim to detect low doses of gamma radiation with fast response time using QTF. Three different types of QTFs (uncoated and gold coated) were used in this study in order to investigate their sensitivity to gamma radiations. Our results show that a thick gold coating on QTF can enhance the quality factor and increase the resonance frequency from 32.7 to 32.9 kHz as compared to uncoated QTF. The results also show that increasing the surface area of the gold coating on the QTF can significantly enhance the sensitivity of the QTF to radiation. We investigated the properties of gold-coated and uncoated QTFs before and after irradiation by scanning electron microscopy. We further investigated the optical properties of SiO2 wafers (quartz) by spectroscopic ellipsometry (SE). The SE studies revealed that even a small change in the microstructure of the material caused by gamma radiation would have an impact on mechanical properties of QTF, resulting in a shift in resonance frequency. Overall, the results of the experiments demonstrated the feasibility of using QTF sensors as an easy to use, low-cost, and sensitive radiation detector.

Dynamic behaviours of the Double-end tuning fork based comb-driven microelectromechanical resonators for modulating the magnetic flux synchronously

MEMS resonators have been widely used in the magneto-resistive (MR) sensor for modulating the magnetic flux to enhance the detection limit. However, the manufacturing tolerances in MEMS fabrication processes make it challenging to fabricate the identical resonators with the same vibration frequency, which greatly decreases the detection limit of the MR sensor. To synchronize the MEMS resonators and improve the performance of the MR sensor, the double end tuning fork (DETF) based comb-driven MEMS resonators is proposed in this paper, making the system operate at the out-of-phase mode to complete the synchronization. The dynamic behaviour of the resonators is investigated through theoretical analysis, numerical solution based on MATLAB code and Simulink, and experimental verification. The results show that the transverse capacitances in the comb will significantly affect the resonance frequency due to the second-order electrostatic spring constant. It is the first time to observe the phenomenon that the resonant frequency increases with the increase of the bias, and it can also decrease with increasing the bias through adjusting the initial space between the fixed finger and the moving mass, they are different from the model about spring softening and spring hardening. Besides, the proposed DETF-based comb-driven resonators can suppress the in-phase and out-of-phase mode through adjusting the driving and sensing ports, and sensing method, meanwhile make the magnetic flux modulation fully synchronized, and maximize the modulation efficiency, and minimize the detection limit. These characteristics are appropriate for the MR sensor, even other devices that need to adjust the resonance frequency and vibration amplitude. Furthermore, the model and the design can also be extended to characteristic the single end tuning fork (SETF) based MEMS resonator and other MEMS-based MR sensors.