scholarly journals Inhibition test of mangosteen peel extract (Garcinia mangostana L.) against bacterial diseases of tiger grouper (Epinephellus fuscoguttatus)

2021 ◽  
Vol 869 (1) ◽  
pp. 012071
Author(s):  
Y Akmal ◽  
R Humairani ◽  
M Muliari ◽  
I Irfannur ◽  
R Rinaldi ◽  
...  
Keyword(s):  
Bacterial Disease ◽  
Garcinia Mangostana ◽  
Bacterial Diseases ◽  
Mangosteen Peel ◽  
Randomized Design ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Net Cages ◽  
Positive Results ◽  
Peel Extract

Abstract Cultivation of tiger grouper (Epinephelus fuscoguttatus) in Floating Net Cages in several locations in Bireuen Regency has developed. The potential disease issue can be caused by bacteria, viruses, fungi, or parasites. The purpose of this study was to determine the effectiveness of mangosteen peel extract capsules (Garcinia mangostana L) against bacterial disease in tiger grouper. This research was carried out in April 2019 at the MIPA Laboratory of Al Muslim University and the laboratory of PT. Centra Proteina Prima, Bireuen Regency. The design used was a completely randomized design with four treatments (0%, 10%, 20%, and 30% mangosteen peel extract capsules) with three replications. The parameters studied in this study including the diameter of the inhibition and the observation of MBC (Minimum Bacterial Concentration). The result showed that the administration of mangosteen peel extract capsules with doses of 10%, 20%, and 30% has no significant effect on reducing disease-causing bacteria in grouper grown on TSA media. Positive results were obtained on positive (+) MBC observations, presumably due to the small dose of mangosteen peel extract capsules given so that it could not kill bacteria grown in dishes with an inhibition range of 0 mm.Introduction

scholarly journals VACCINATION OF JUVENILE TIGER GROUPER WITH THREE INACTIVATED BACTERIAL PATHOGENS

2016 ◽  
Vol 7 (2) ◽  
Author(s):  
. Zafran
Keyword(s):  
Bacterial Infection ◽  
Sea Water ◽  
Control Fish ◽  
Bacterial Disease ◽  
Bacterial Vaccine ◽  
Total Length ◽  
Photobacterium Leiognathi ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Net Cages

An experiment to evaluate the efficacy of inactivated polyvalent bacterial vaccines to increase specific immunity of tiger grouper (Epinephelus fuscoguttatus) against bacterial infection was conducted in floating net cages located in Sumbawa Island, West Nusa Tenggara. Polyvalent bacterial vaccine was prepared by mixing three species of killed-bacteria (Vibrio harveyi, V. alginolyticus, and Photobacterium leiognathi) with ratio of 1:1:1. The density of bacterial vaccine was adjusted at 1010 CFU/ml. The vaccines were delivered by immersion. The control fish were immersed in sea water without vaccine. Fish with ± 6 cm of total length and ± 7 g of body weigth were used in this experiment. The fishes were reared in concrete tanks in hatchery of Gondol, Bali until they reached ± 9 cm of total length. Later, the fishes were reared for 6 months in floating net cages located in Sumbawa. Re-vaccinations were delivered 60 days and 120 days post initial vaccination. The results showed that titer antibody and survival rate of the vaccinated fish were 1/128 and 77.11%, higher than the control (titer antibody=1/4-1/8, survival=45.56%, respectively). The relative percent survival of the vaccinated fish was 57.95%. It was suggested that the vaccines were immunogenic and had protective ability against bacterial infection in tiger groupers. Keywords: bacterial disease, polyvalent bacterial vaccine, tiger grouper

scholarly journals FORMULASI NANOEMULGEL EKSTRAK KULIT MANGGIS (Garcinia Mangostana L.)

2019 ◽  
Vol 1 (3) ◽  
pp. 166-176
Author(s):  
Hilda Damayanti ◽  
Saleh Wikarsa ◽  
Garnadi Jafar
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Dpph Method ◽  
Nanoemulsion Gel ◽  
Materials Used ◽  
Peel Extract

Antioxidant-containing cosmetic has antiaging therapy that can inhibit the free radical formation. Mangosteen peel extract has very strong antioxidant activity. To enhance the effect and comfortness of mangosteen peel extract use on the skin, it could be made into nanoemulgel. The article provides the information about method of preparation and evaluation of nanoemulsion-gel. The purpose of this study was to formulate a stable microemulgel of mangosteen peel extractusing halal materials declared halal according to Islamic Shari’a.  the materials used don’t contain carrion, blood, pig and/ animals that don’t conform to Islamic Shari’a. Microemulgel mangosteen peel extract was made by varying plantacare® 1200 UP concentration as cosurfactant (5, 10, 15, 20 and 25%) in the microemulsion and it was incorporated into the gel base. Evaluations were included the antioxidant activity test and organoleptic, pH, viscosity, stability, particle size analysis and panelist test. The antioxidant activity determined by DPPH method showed that IC50 value of mangosteen peel extract was 5.54 ppm. The third microemulsion formula containing cosurfactant of 15% resulted in the best results in that the parameter of the product can be penetrated by ray laser was at particle size of  23.65 nm, was determined by tranmission Electron Microscopy (TEM). Microemulgel containing Viscolam® MAC 10 of seven percent gave the stable formula proofed by freeze thaw and sentrifuga test. The five microemulgel formulations were stable.    

scholarly journals UJI EFEK ANALGESIK EKSTRAK KULIT MANGGIS (GARCINIA MANGOSTANA L.) PADA MENCIT SWISS (MUSS MUSCULUS)

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Ripka Margaretha Ponggele
Keyword(s):  
Control Group ◽  
Test Substance ◽  
Reference Solution ◽  
Garcinia Mangostana ◽  
Hot Plate ◽  
Plate Method ◽  
Analgesic Effects ◽  
Mangosteen Peel ◽  
Pain Stimuli ◽  
Peel Extract

Background:Indonesia is the country with the second largest plants after Brazil. One of the plants that grow in Indonesia is the mangosteen plant is utilized as a medicine by the people of Indonesia but also by people in some other countries. Mangosteen skin is most often used to treat diseases such as fever, diarrhea, hypertension, antioksidant, antibiotics, anti-inflammatory, and many other diseases. This study aims to prove the presence or absence laboratorik analgesic effects of mangosteen peel extract in mice.Methods:This study uses a sample of nine experimental Swiss mice. The sample was divided into 3 groups, namely the positive control group given the drug tramadol, negative control group given distilled water control and experimental groups were given mangosteen peel extract, each group consisted of three mice. Testing is done by providing analgesic effects of pain stimuli using thermal stimuli (hot plate method) with a temperature of 550C. Given pain stimuli will cause the mice were protecting themselves by jumping response or lick the feet and tail. Testing efekan algesik done before giving the test substance and the reference solution, then at minute 30,60,90 and 120 minutes after administration of the test substance and the reference solution. Tests carried out for 1 minute.Results:mangosteen peel extract has analgesic effects are starting to look at minute 30 to minute 120 with the maximum effect seen at minute 90.Conclusion:mangosteen peel extract (Garcinia mangostana, L) has analgesic effect in swiss mice (Mus musculus)Keywords: Test the effects of analgesics, the mangosteen peel extract, mangosteen skinAbstrakIndonesia merupakan negara yang mempunyai tumbuh-tumbuhan terbesar kedua setelah Brasil. Salah satu tumbuhan yang tumbuh di Indonesia adalah tanaman manggis yang sudah dimanfaatkan sebagai obat oleh penduduk Indonesia bahkan oleh penduduk di beberapa negara lain. Kulit manggis yang paling sering digunakan untuk mengobati penyakit seperti demam, diare, hipertensi, antioksidant, antibiotik, antiinflamasi, dan masih banyak penyakit lainnya. Penelitian ini bertujuan untuk membuktikan secara laboratorik ada tidaknya efek analgesik dari ekstrak kulit manggis pada mencit.Metode : Penelitian ini menggunakan metode eksperimental dengan sampel 9 ekor mencit Swiss. Sampel di bagi dalam 3 kelompok, yaitu kelompok kontrol positif yang diberikan obat tramadol, kelompok kontrol negatif diberikan aquades dan kelompok kontrol eksperimen yang diberikan ekstrak kulit manggis, yang masing-masing kelompok terdiri dari 3 ekor mencit. Pengujian efek analgesik dilakukan dengan memberikan rangsang nyeri menggunakan metode rangsang panas (hot plate method) dengan suhu 550C. Rangsang nyeri yang diberikan akan menyebabakan mencit melakukan perlindungan diri dengan respon melompat atau menjilat kaki dan ekor. Pengujian efek analgesik dilakukan sebelum pemberian zat uji dan larutan pembanding, kemudian pada menit ke 30,60,90 dan menit ke 120 setelah pemberian zat uji dan larutan pembanding. Pengujian dilakukan selama 1 menit.Hasil : Ekstrak kulit manggis mempunyai efek analgesik yang mulai terlihat pada menit ke 30 sampai menit ke 120 dengan efek maksimalnya terlihat pada menit ke 90.Kesimpulan : Ekstrak kulit manggis (Garcinia mangostana, L) memiliki efek analgesik pada mencit swiss (Mus musculus).Kata Kunci : Uji efek analgesik, ekstrak kulit manggis, kulit manggis.

scholarly journals Penambahan Asam Amino Taurin pada Pakan Buatan terhadap Peningkatan Pertumbuhan dan Sintasan Benih Ikan Kerapu Cantik (Epinephelus fuscoguttatus × Epinephelus microdon) [Addition of Amino Taurine Acid to Artificial Feed on Increased Growth and Survival Rate of Cantik grouper Seed (Epinephelus fuscoguttatus × Epinephelus microdon)]

2018 ◽  
Vol 10 (2) ◽  
pp. 112
Author(s):  
Nizar Afiansyah Loekman ◽  
Woro Hastuti Satyantini ◽  
Akhmad Taufiq Mukti
Keyword(s):  
Survival Rate ◽  
Control Variable ◽  
Growth And Survival ◽  
Randomized Design ◽  
Hybrid Grouper ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Completely Randomized Design

AbstrakKerapu cantik merupakan kerapu hibrid hasil persilangan antara betina kerapu macan dan jantan kerapu batik. Salah satu permasalahan dalam pembenihan kerapu cantik secara umum, yaitu pertumbuhan stadia awal benih kerapu cantik yang lambat. Permasalahan tersebut dapat diatasi dengan menambahkan taurin pada pakan buatan untuk meningkatkan pertumbuhan benih ikan kerapu cantik.Penelitian ini bertujuan untuk mengetahui pengaruh penambahan taurin pada pakan buatan terhadap pertumbuhan dan sintasan benih ikan kerapu cantik. Penelitian ini menggunakan metode RAL dengan tiga perlakuan, yaitu pemberian pakan buatan dengan kadar taurin 0% (P1), 0,5% (P2) dan 1% (P3) yang diberikan pada benih ikan kerapu cantik dan masing-masing perlakuan diulang enam kali. Hasil pemeliharaan selama 45 hari menunjukkan bahwa penambahan taurin sebesar 1% pada pakan buatan memberikan pertumbuhan tertinggi dibandingkan perlakuan lainnya dan berbeda nyata (p<0,05). Penambahan taurin 1% pada pakan buatan tidak memberikan hasil sintasan yang berbeda dengan kontrol (taurin 0%). AbstractCantik grouper is hybrid grouper, it is the results of a crossover between females tiger grouper and males batik grouper. One of the problems in breeding cantik grouper in common is the growth of early stadia juvenile cantik grouper is slow. This problem could be solved by added taurine in the feed to increase the growth of juvenile cantik grouper. The purpose of this research is to find the influence of the addition of taurine in the feed on the growth and survival rate of juvenile cantik grouper. This research used completely randomized design (CRD) method with three treatments, which is giving taurine to feed with 0 % (P1), 0.5 % (P2) and 1 % (P3) concentration given to the juvenile cantik groupers and the treatment has been repeated six times. The results of breeding for 45 days showed that the addition of 1 % taurine in feed give the highest growth than other treatment and differs markedly ( p<0.05 ). The addition of 1% taurine gives no different result with the control variable (0% taurine).

scholarly journals Kajian kinerja pertumbuhan dan kelangsungan hidup ikan kerapu macan (Ephinephelus fuscogusttatus) menggunakan pakan hewani yang berbeda

2021 ◽  
Vol 8 (2) ◽  
pp. 118
Author(s):  
Munawwar Khalil ◽  
Salamah Salamah ◽  
Zumairi Zumairi ◽  
Muliani Muliani
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Feed Conversion ◽  
Live Feed ◽  
Highest Weight ◽  
Randomized Design ◽  
Length Increase ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Completely Randomized Design

The tiger grouper (Epinephelus fuscoguttatus) is an economically important fish whose fry still relies on natural catches. Several species of grouper are very potential to be cultivated because of their fast growth, efficient feed conversion, and high selling value. The purpose of this study is growth performance and survival rate tiger grouper (Epinephelus fuscoguttatus) used different live feed. The research design used in this study was a Completely Randomized Design with 3 treatments 3 replications and continued with LSD test if there were differences. The treatments given are treatment A (Snail), treatment B (Chicken Intestine), and treatment C (Caterpillar Maggot). Parameters observed were length increase, weight gain, survival, feed response, and water quality. The results of this study indicate that the highest increase was found in the treatment of C: 4,04 cm and the lowest was in treatment B: 3 cm, the highest weight gain was found in the treatment of C: 3,73 grams, and the lowest was in treatment B: 2,18 grams. The survival rate for all treatments is 100%. During this study the temperature ranged (28.6-31.8 oC), pH (6.7-8.2), DO (6.3-8.1 ppm), and salinity (23-27 ppt).Keywords: growth, live feed, survival rate, tiger grouper.

scholarly journals Moina sp. Powder Supplementation as Artemia sp. Substitute Through Growth, Lysine, Histidine, Methionine, and Leucine Amino Acid Contents in Tiger Grouper x Camouflage Grouper Hybrid Larvae (Epinephelus fuscoguttatus x Epinephelus microdon)

Omni-Akuatika ◽  
2018 ◽  
Vol 14 (3) ◽  
Author(s):  
Shobrina Silmi Qori Tartila ◽  
Shobrina Silmi Qori Tartila ◽  
Arga Iswara ◽  
Frida Choirun Nisa' ◽  
Nofita Irmayani Herlambang ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Salinity ◽  
Larval Phase ◽  
Live Feed ◽  
Weight Growth ◽  
Randomized Design ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Amino Acid Contents ◽  
Good Substitute

Tiger Grouper x Cammouflage Grouper (TGCG) (Epinephelus fuscoguttatus x Epinephelus microdon) production is still low nowadays due to the insufficient price of the live feed supply during the larval phase, which is nauplii Artemia sp. One of the live feed types which is able to become a substitute of nauplii Artemia sp. is Moina sp., which has better lysine, histidine, methionine, and leucine contents, besides having the sufficient price for the supply itself. However, Moina sp. is unable to survive in the high salinity, thus it has to be produced as a powder. This condition leads to a further research whether to observe that the Moina sp. powder supplementation is able to become nauplii Artemia sp. substitute through the increased growth and the optimum better lysine, histidine, methionine, and leucine contents in TGCG larvae. The research was conducted using the complete randomized design experimental method. The result showed that Moina sp. powder is compatible as good substitute candidate of nauplii Artemia sp., as it increased the larval length growth, even it had no difference in weight growth, but it was also able to increase the larval lysine, histidine, methionine, and leucine contents.

scholarly journals Uji Daging Ikan Mujair (Oreochromis mossambicus) Menggunakan Rendaman Kulit Manggis (Garcinia mangostana L.)

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Eka Kusmawati ◽  
Hari Santoso ◽  
Ahmad Syauqi
Keyword(s):  
Research Method ◽  
Oreochromis Mossambicus ◽  
Effective Concentration ◽  
Test Results ◽  
Real Difference ◽  
Garcinia Mangostana ◽  
Fish Meat ◽  
Mangosteen Peel ◽  
Randomized Design ◽  
Completely Randomized Design

Meat elasticity is a characteristic of meat that has the power to resist pressure from the outside and return to its original shape. Tilapia fish (Oreochromis mossambicus) has perishable properties if it hasn’t been through processing. One alternative to maintain the suppleness of the meat by using a soaking mangosteen (Garcinia mangostana L.) peel. Based on the description above this study aims to knowing the effectiveness of soaking using mangosteen peel and analyzes the concentration for the best meat suppleness. The research method used was completely randomized design using 4 concentration of treatments 0%, 5%, 10%, 15% and  each with 5 replications. The results of study obtained Fcou = 72,55 ; F(0,01) = 5,29 shows that Fcou > F(0,01) so H0 is rejected, which means there is a very real difference between treatments with value concentration of 0% 5,069 N/mm2; concentration of 5% 7,574 N/mm2; concentration of 10% 10,401 N/mm2 and  concentration of 15% 13,924 N/mm2. The secondary metabolites in the mangosteen peel are effective so as to give effect to increase the tenderness of tilapia fish and the test results of the effective concentration value for the suppleness of the best fish meat according to SNI is the concentration of 15%. In this elascity test it was found thet the higher of concentration of mangosteen peel given the higher the level of elasticity of tilapia fish meat.Key Word: Elasticity, Immersion, Powder ConcentrationABSTRAKKekenyalan daging merupakan sifat daging yang mempunyai kekuatan untuk menahan tekanan dari luar dan kembali ke bentuk semula. Daging ikan mujair (Oreochromis mossambicus) memiliki sifat mudah rusak jika belum melalui suatu pengolahan. Salah satu alternatif untuk menjaga kualitas kekenyalan dagingnya dengan menggunakan rendaman kulit buah manggis (Garcinia mangostana L.). Tujuan penelitian ini adalah untuk mengetahui efektifitas perendaman menggunakan kulit buah manggis dan menganalisa konsentrasi yang tepat untuk kekenyalan daging ikan mujair. Metode penelitian yang digunakan yaitu Rancangan Acak Lengkap menggunakan 4 konsentrasi perlakuan 0%, 5%, 10%, 15% dan masing-masing 5 kali ulangan. Hasil penelitian didapat Fhit = 72,55 ; F(0,01) = 5,29 menunjukkan bahwa Fhit > F(0,01) sehingga H0 ditolak yang berarti terdapat beda sangat nyata antar perlakuan, dengan nilai konsentrasi 0% 5,069 N/mm2; konsentrasi 5% 7,574 N/mm2; konsentrasi 10% 10,401 N/mm2 dan konsentrasi 15% 13,924 N/mm2. Senyawa metabolit sekunder pada kulit manggis yang efektif sehingga memberikan pengaruh untuk meningkatkan kekenyalan daging ikan mujair dan hasil uji nilai konsentrasi yang efektif untuk kekenyalan daging ikan terbaik sesuai dengan SNI adalah konsentrasi 15%. Pada uji kekenyalan ini didapatkan bahwa semakin tinggi konsentrasi kulit manggis yang diberikan maka semakin tinggi tingkat kekenyalan daging ikan mujair.Kata Kunci: Kekenyalan, Perendaman, Konsentrasi Serbuk

scholarly journals The inhibitory efficacy of flavonoid of mangosteen peel extract (Garcinia Mangostana Linn.) against Lactobacillus Acidophilus biofilm bacteria

2020 ◽  
Vol 10 (2) ◽  
pp. 75
Author(s):  
Dyah Utari Wahyu Ningrum ◽  
Karlina Samadi ◽  
Widya Saraswati
Keyword(s):  
Treatment Group ◽  
Dental Caries ◽  
Lactobacillus Acidophilus ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Biofilm Bacteria ◽  
Peel Extract

Background: Lactobacillus takes role in the formation of dental plaque which is the main factor that cause dental caries. The bacteria take role in the metabolism of glucose in the mouth, producing organic acids which lower the pH in the oral cavity. This situation can lead to the formation of dental caries because these bacteria can form biofilm as a defense of microorganisms to antibiotics and the immune response. Mangosteen peel has active ingredients such as flavonoid that can be used to inhibit biofilm. The ability of flavonoid compounds in the phenol group can make the bacterial enzyme becomes inactive, causing the activity of glucosyltransferase enzyme that usually used by bacteria to synthesize sucrose in the medium becomes glucan. As a result, bacterial biofilm formation is inhibited because the amount of glucan as a medium of bacterial attachment is limited, but until now the effectivity dose of flavonoid from mangosteen peel extract (Garcinia mangostana Linn.) is has not known yet Purpose: The aim of this study was to determine the inhibitory efficacy of flavonoid of mangosteen peel extract against the formation of Lactobacillus acidophilus bacterial biofilm. Methods: Bacteria that had been formed into biofilms was studied in two times treatment, one concentration reviewed by flavonoid from mangosteen peel extract and without reviewed by flavonoid from mangosteen peel extract. The treatment group was incubated at 37°C until day 8 since the first day incubated. The treatment group was washed with phosphate buffered saline (PBS) 4 times and dried. The treatment group stained with 0.2 ml 0.1% crystal violet and 15 min incubation. The treatment group was rinsed with distilled water 3 times and then dried. Solvent DMSO 100% was added as much as 0.1 ml in each well. Microtiter plate was shaken for 1 minute and then placed in to the microplate reader and then the OD (Optical Density) can be read. Results: There were significant differences between the inhibitory efficacy of flavonoid of mangosteen peel extract against Lactobacillus acidophilus biofilm bacteria, the control group and group treatments (p <0.05). Conclusion: Flavonoid of mangosteen peel extract with the concentration of 0.78% had inhibitory effect against Lactobacillus acidophilus biofilm bacteria

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