scholarly journals Evaluation of Proximate and Mineral Composition of Mutant Dolichos Lablab (Lablab purpureus L.) Accessions in Kenya

Author(s):  
S. K. Kimno ◽  
M. G. Kinyua ◽  
K. C. Pkania ◽  
E. Chepkoech

Plant breeding through induced mutation technology is a potent method   to creating new variants of food crops with of desirable phenotypic, genetic and biochemical functions. It is a catalyst in developing improved crop varieties where classical hybridization or selection have limitations. It has been used to improve nutrition quality and higher yield in a number of legumes. Dolichos Lablab (Lablab purpureus L) is multipurpose legume that has not been exploited extensively for food nutritional properties through breeding. The purpose of the study was, therefore, to generate awareness that nutritional status of D. Lablab could be improved through mutation induction and be a good source of food components essential for good health. Twenty-four dolichos Lablab germplasms including 20 mutant accessions and 4 commercial genotypes were evaluated for proximate values and mineral contents in Kenya in 2021 based on Association of Official Analytical Chemists (AOAC). Data analysis  was  based on least significant difference (P = .05).The   revealed  percent moisture,  ash crude fat,   crude fibre, crude proteins and total starch content ranged :  7.35% -11.84%, 1.09 -2.90%, 3.35% - 8.05% , 8.86 - 12.70% , 20.03% - 28.87% and 25.50% - 39.00% .The  mineral content of phosphorous (P), potassium(K) and calcium (Ca) ranged from  27mg - 57mg,  132mg -297mg  and  7mg - 19mg. A significant positive correlation between ash levels to P and K concentration and on P to K concentration. The result of analysis  ranked  9  (WT026, WT018 ,MT110 , BT188 , BT032, BT114, MT076 BT137,GT09) different  accessions  that positively  contributed to the nutritional content of the investigated  dolichos lablab accessions. Further research on the superior accessions can be done on yield potential, resistance to biotic and abiotic constrains, sensory preferences or used in bio fortification of existing genotypes.

2021 ◽  
Vol 38 (2) ◽  
pp. 57-66
Author(s):  
G. S. Bawa ◽  
S. O. Ogundipe ◽  
T. S. B. Tegbe ◽  
I. I. Dafwang

This study investigated the effects of varying the duration of cooking on the nutrient composition and levels of some antinutritional factors in lablab seeds. Raw lablab seeds were subjected to four durations of cooking, viz: 15, 30, 45 or 60 minutes respectively, in a drum of boiling water. The samples were dried and assayed for their proximate compositions, mineral contents, presence and levels of trypsin inhibitors (TIA), phytic acid, tannin and hydrocyanic acid (HCN). The raw lablab seeds contained 94.03% dry matter (DM), 26.12% crude protein (CP), 7.86% crude fibre (CF), 2.02% ether extract (EE), 4.43% ash and 59.57% nitrogen free extract (NFE). The proximate composition was not significantly (P>0.05) affected by duration of cooking. Potassium (15.66g/Kg DM) and iron (245.17g/Kg DM) were the most abundant macro and micro mineral elements, respectively in the raw lablab seeds while sodium (0.06g/Kg DM) and copper (53.62 mg/kg DM) were the least for macro and micro minerals, respectively. Except for calcium and sodium, all the minerals assayed were leached significantly (P<0.05) during cooking, Cooking lablab seeds at 100°C for forty-five minutes decreased trypsin inhibitor activity (TIA) from 593.87 mg/100g to 132.00 mg/100g; phytic acid from 5.65 mg/100g to 0.10 mg/100g; tannin from 0.22 mg/100g to 0.10 mg/100g and hydrocyanic acid (HCN) from 1.58 mg/100g to 0.45 mg/100g, respectively. These values correspond to 77.77, 78.93, 54.55 and 71.52 percent reductions in TIA, phytic acid, tannin and hydrocyanic acid, respectively. Cooking lablab seeds for 45 minutes appear to be the optimum for elimination of these antinutrients from lablab seeds.  


2021 ◽  
Vol 39 (1) ◽  
pp. 180-189
Author(s):  
F. T Ajayi

This study assessed the dry matter yield, mineral and proximate composition of Panicum maximum Jacq var. Ntchisi planted with Lablab purpureus (Lablab), Centrosema pubescens (Centro), Aeschynomene histrix (A. histrix) and Stylosanthes guianensis (Stylo) in a 3 x 5 factorial experiment using Randomized Complete Block Design in early, mid and late rainy season. The dry matter yields (DMY) of panicum sown with Lablab (11.82 t/ha/yr) was significantly higher (P<0.05) than that observed for other mixtures. The least DMY of 9.46 t/ha/yr was observed for panicum in panicum/ A. histrix mixture. The yields recorded for Stylo and Lablab were highest in mid and late rainy seasons than in the early rain. Panicum sown with Lablab had the highest DM (37.71g/100g DM), Crude protein (CP) (11.36g/100g DM), ether extracts (7.20g/100g DM) and ash content (2.52g/100g DM). Among the legumes, Lablab had the highest CP (23.29 g/100g DM), crude fibre (10.61 g/100g DM) and ash content (3.96 g/100g DM). However, Centro was highest in ether extract (9.30g/100g DM). Panicum sown with Lablab had higher concentration of sodium (Na) and iron (Fe) while panicum planted with Stylo had highest concentration of phosphorus (P). The concentration of calcium (Ca) was similar (P>0.05) among the mixtures but differed (P<0.05) in the legumes. Stylo had the highest Ca concentration (0.72%) whereas highest P and Fe values were observed in Lablab. In conclusion, panicum harvested from panicum/lablab mixture had the highest DMY, CP, EE and ash content of grass in early, mid and late rainy season than the other mixtures.   


Author(s):  
Oluwafemi Akinsola Omole ◽  
Oluwafunmike Blessing Adepoju ◽  
Oluwasola Toluwalope Moradeyo ◽  
Olayinka Josephine Okuneye ◽  
Adunola Abosede Bello

The nutritive value of smoked, dried Procambarus clarkii sourced from Akwa Ibom, Rivers and Lagos states of Nigeria were evaluated and compared.  The proximate composition of the crayfish samples were determined using official methods of analysis, mineral composition were determined using Atomic Absorption Spectrophotometer and  the amino acid composition were analysed using Amino Acid Analyser. The proximate composition of the crayfish samples from three different locations (Akwa Ibom, Lagos and Rivers states) such as moisture, crude protein, crude fat and ash contents showed significant difference (p<0.05) across the selected locations while crude fibre and carbohydrate content showed no significant difference (p<0.05) across the selected locations. The amino acids composition gave the crayfish sample from Akwa Ibom state as the richest source of essential amino acids. The mineral contents of the crayfish samples such as potassium, phosphorus, magnesium, manganese and calcium showed significant difference (p<0.05) across the selected locations while calcium, zinc and sodium contents showed no significant difference (p<0.05) across the selected locations. Comparing their nutrient component, smoked, dried P. Clarkia from Awka Ibom state possess the richest nutritive value. 


Author(s):  
Opeh, Patience Bassey

The food and mineral contents of flesh of the two bivalve species (Egeria radiata and Crassostrea rhizophorea) of Cross river, Nigeria were investigated. The study revealed that the moisture content of E. radiata and C. rhizophorea was 52.46% and 54.01%, crude protein was 24.37% and 20.49%, fat was 9.03% and14.22%, crude fibre was 0.01% and 1.30%, ash was 1.43% and 9.05%, carbohydrate was 12.7% and 2.23% and energy was 229.58J and 218.84J. The mineral content of E. radiata and C. rhizophorea  showed a significant difference (P<0.05) in Ca+, Na+, K, Mg, while the Zn, Pb, Cu, Fe, P and Mn were similar (P>0.05). Based on the findings of this study, biochemical profile of E. radiata is high in nutrients and is suitable for human consumption. Therefore, concerted efforts should be made to enlighten the populace on the increased nutritional benefits of consuming E. radiata.


2013 ◽  
pp. 61-65
Author(s):  
Ágnes Krivián

The comparative trial has been set up in the Demonstration Garden of the Institute of Crop Sciences of the University of Debrecen, Centre for Agricultural and Applied Economic Studies, Faculty of Agricultural and Food Sciences and Environmental Management in 2012, with 24 hybrids with different genetic characteristics and growing periods. The soil of the trial is lime-coated chernozem, with a humus layer of 50–70 cm. The weather of the trial year was quite droughty; the monthly average temperature was 3–4 oC higher than the average of 30 years. High temperature, together with lack of precipitation occurred during the most sensitive phenophases of maize (flowering; fecundation, grain saturation). The following characteristics have been observed: starting vigour, date of male and female flowering, plant and cob height, dry-down dynamics during maturation and the change of yield composing elements has also been quantified. The yield was recalculated to 14% moisture content grain yield after harvesting. The beginning of the growing period was advantageous, therefore the analysed hybrids could grow a high (above 300 cm) and strong stem. The yield of the hybrids changed between 10.33 and 11.87 t ha-1, but as a result of the unfavourable climatic extremes, their genetic yield potential prevailed only at a rate of 30–40%. However, moisture content by the time of harvesting was good despite its early date (12th September); it remained under below 14% in most cases. Dry-down was measured on a weekly basis between 14th August and 5th September. The analysis of the qualitative parameters of the maize hybrids (protein %, oil % and starch %) resulted in significant differences. The most significant difference has been observed in the case of protein content (LSD5%=2.01). Oil content was the most advantageous in the case of hybrids belonging to the mid-late growing group (FAO 400). The X9N655 and 36V74 hybrids had the highest oil content (around 4%), while hybrids P9915 and 37F73 had significantly lower oil content. Starch content was above 70% in the case of every hybrid. Hybrid selection is highly important in terms of yield and yield security of maize, as well as the application of modern biological fundamentals and hybrid specific technology for the improvement of the level of cultivation technology.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yerong Zhu ◽  
Xiaoxue Li ◽  
Xuan Gao ◽  
Jiqi Sun ◽  
Xiaoyuan Ji ◽  
...  

Abstract Background Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. The starch content can be promoted by plant growth regulators after the vegetative reproduction being inhibited. Maleic hydrazide (MH) has been reported to inhibit plant growth, meantime to increase biomass and starch content in some plants. However, the molecular explanation on the mechanism of MH action is still unclear. Results To know the effect and action mode of MH on the growth and starch accumulation in Spirodela polyrrhiza 7498, the plants were treated with different concentrations of MH. Our results showed a substantial inhibition of the growth in both fronds and roots, and increase in starch contents of plants after MH treatment. And with 75 µg/mL MH treatment and on the 8th day of the experiment, starch content was the highest, about 40 mg/g fresh weight, which is about 20-fold higher than the control. The I2-KI staining and TEM results confirmed that 75 µg/mL MH-treated fronds possessed more starch and big starch granules than that of the control. No significant difference for both in the photosynthetic pigment content and the chlorophyll fluorescence parameters of PII was found. Differentially expressed transcripts were analyzed in S. polyrrhiza 7498 after 75 µg/mL MH treatment. The results showed that the expression of some genes related to auxin response reaction was down-regulated; while, expression of some genes involved in carbon fixation, C4 pathway of photosynthesis, starch biosynthesis and ABA signal transduction pathway was up-regulated. Conclusion The results provide novel insights into the underlying mechanisms of growth inhibition and starch accumulation by MH treatment, and provide a selective way for the improvement of starch production in duckweed.


2020 ◽  
Vol 24 (4) ◽  
pp. 639-643
Author(s):  
M.M. Ogunbambo

Smoke-drying Clarias gariepinus (catfish) provides animal protein and a source of livelihood in Lagos, Nigeria. Changes occurring to seasonal and mineral compositions of smoke-dried catfish using local Traditional Drum Kiln (TDK) and a newly constructed Eco-Friendly Kiln (EFK) fitted with a flame, drying and electronic components was carried out in this study. The smoke-drying process was carried out in both wet and dry seasons and smoke-dried catfish samples stored at ambient and adjusted refrigerated temperatures of 28 and 4 0C. The smoke-drying procedure was  standardized at 60 - 80 0C and kiln lasted 24 ± 3 hours. Moisture content results showed a significant difference when the catfish samples were smoke-dried using TDK and EFK and stored in ambient and controlled temperatures in both wet and dry seasons while crude protein, lipid, ash and crude fibre values were significantly different when stored only in dry season. Mineral elements phosphorus, sodium, copper, magnesium and iron showed a significant difference when stored at both temperatures and seasons using both kilns. Mineral elements were found to be most stable in smoke-dried catfish samples when stored at controlled temperatures. This work proved that standardizing smoke-drying process using both kilns resulted in good quality smoke-dried catfish but showed that higher biochemical values were obtained when EFK is used. Key words: Nigeria, Smoke-drying Kilns, Seasons, Ambient, Controlled Temperatures


1978 ◽  
Vol 58 (4) ◽  
pp. 743-752 ◽  
Author(s):  
J. J. KENNELLY ◽  
F. X. AHERNE ◽  
A. J. LEWIS

Forty-eight crossbred pigs of average initial weight 21 kg were fed 10% Tower rapeseed meal (RSM) and 10% Candle RSM as partial replacements for soybean meal (SBM). Diets were formulated to be isocaloric. Pigs fed the SBM diet consumed less feed, gained significantly (P < 0.01) faster and were more efficient at converting feed to gain than those fed the RSM diets. Performance of pigs fed Candle RSM was not significantly different to that obtained with Tower RSM. In a second experiment, dehulled Tower RSM and Tower RSM hulls were mixed in amounts to produce RSM with crude fibre levels of 6.8, 10.8, 13.5 and 15.8%. The simulated RSM and Tower and Candle RSM were used to completely replace SBM in the diets of weanling (75 g) Sprague-Dawley rats. Rats fed SBM had significantly (P < 0.05) higher average daily gain (ADG) than those fed Tower or Candle RSM, or diets containing the rapeseed meats. There was no significant (P < 0.05) difference in ADG, feed intake or feed to gain ratio of rats fed either Tower or Candle RSM. Feed intake, feed to gain ratio and fecal volatile fatty acid concentrations increased while average daily gain decreased with increasing level of hulls in simulated RSM diets. There was no significant difference (P < 0.05) in thyroid weight between rats fed SBM, Tower RSM or Candle RSM.


2020 ◽  
Vol 18 (1) ◽  
pp. 77-89
Author(s):  
J.N. Ingweye ◽  
J. Mfon ◽  
G.A. Kalio ◽  
F.I. Ologbose

Performance and organoleptic indices of rabbits fed plantain, oil palm and calopogonium leaves was assessed using 48, eight weeks old bucks. They were allotted to four groups of 12 animals and three replicates of four animals each. Group A (control) was fed calopogonium leaf while groups B, C, and D were fed plantain leaf, oil palm leaf, and 50% oil palm + 50% plantain leaves, respectively. Animals consumed fixed amount of commercial pellets while forages and water were given ad libitum. Completely randomized design, one-way analysis of variance was used. Proximate analysis of pellets and forages as well as feed intake, weight gain and feed conversion ratio were assessed. At the end of the experiment, meat from one rabbit per treatment was used for sensory evaluation by 20 semi-trained panelists using 7-point hedonic scale. Data were analyzed using SPSS software. Significant means were separated using Least Significant Difference. Crude protein, crude fibre, ether extract, nitrogen free extract and ash contents of the leaves ranged from 25.11±1.50 (calopogonium) to 4.90±0.92 (oil palm), 38.40±2.12 (oil palm) to 15.38±0.07 (plantain), 9.71±0.01 (plantain) to 2.30±0.01 (oil palm), 47.80±0.56 (oil palm) to 32.27±0.02 (calopogonium) and 11.93±0.02 (plantain) to 2.99±0.01 (oil palm), respectively. Groups A and B had the highest (p<0.05) final weight and weight gains while groups A, B and C had the highest (p<0.05) forage intake. Feed conversion ratios of groups A and B were better (lower) than others. Forages had no effect on meat colour and texture. Groups B and D meats tasted better than others. Group D meat aroma was better than those fed single forages. Meats from groups C and D were the juiciest while meats from groups A, C and D were the most acceptable. Consideration of final weights, weight gains, feed conversion ratio and taste of meat would require that plantain leaves be used to feed meat rabbits. Keywords: Organoleptic indices, Dry season feeding, Forage


Author(s):  
Cromwell Mwiti Kibiti ◽  
Anthony Jide Afolayan

Background: B. abyssinica is a succulent member of the genus Bulbine (Asphodelaceae). It occurs from the Eastern Cape, through Swaziland and further north to Ethiopia. The species is used in traditional medicine to treat rheumatism, dysentery, bilharzia, cracked lips and diabetes. The tea leaf is used to treat cough, vaginal and bladder problems. Whereas B. abyssinica has ethno medicinal value, not much data concerning its phytonutrient, macro and micro element composition can be found in literature. Materials and Methods: Therefore, the present study was undertaken to determine the nutritional quantitative composition of the plant using standard procedures. Results: The proximate analysis revealed the carbohydrate, crude fibre, moisture, ash, crude protein and crude fat contents as 74.8%, 8.9%, 8.8%, 8%, 7.7% and 0.6%, respectively. The species showed high levels of oxalates and phytic acids, moderate levels of alkaloids, flavonoids, saponins and phenols, while tannins were in low levels. Vitamin A, C and E contents were 12, 12.3 and 22.1 mg/100g, respectively. Amongst the mineral elements investigated, potassium and calcium were in high levels. Magnesium, iron, sodium, aluminium and phosphorus were moderately present, while manganese, zinc and copper where in low amounts. These vitamins and mineral elements were within their recommended daily allowance in humans. Conclusion: The amount of these phytochemicals suggests the plant can serve as nutritional supplements which are vital in maintaining good health status. These findings also suggest the potential role of B. abyssinica in the treatment of infections and some chronic diseases, especially diabetes mellitus.


Sign in / Sign up

Export Citation Format

Share Document