As a type of flap valve evolved from integral flap valve, two-stage flap valve has the advantages of large opening angle, small hydraulic loss and small impact force on the flap valve seat when the flap valve is closed. In order to analyze and study the hydraulic loss characteristics of the two-stage flap valve, this paper takes a pump station as an example. Based on theoretical analysis, combined with numerical simulation and model test, the hydraulic loss of two-stage flap valve is studied, and the relationship between hydraulic loss and pump station flow is obtained. According to the test results, the hydraulic loss of two-stage flap valve increases with the increase of flow rate under the same opening angle of flap valve. Under the same flow condition, the larger the opening angle of the flap valve is, the smaller the hydraulic loss of the two-stage flap valve is. When the opening angle of the upper flap valve is greater than 46° and the opening angle of the lower flap valve is greater than 64°, the hydraulic loss is less than 70mm and tends to be stable. The influence of hydraulic loss on the performance of pump device is gradually weakened. The relationship between hydraulic loss and flow of two-stage flap valve no longer satisfies the relationship of square under the constant opening angle. Moreover, the larger the opening angle of the two-stage flap valve is, the greater the relationship between hydraulic loss and flow is. Compared with the integral flap valve, the two-stage flap valve has better structural form and hydraulic characteristics, and has little influence on the performance of the pump device, which can provide reference for the application of the two-stage flap valve in the pump station.