Retinal nerve fiber layer thickness and retinal vascular caliber alterations in coal miners in northern China: a community-based observational study

AIM: To evaluate retinal nerve fiber layer thickness and retinal vascular caliber alterations in coal mine workers. METHODS: The community-based observational cross-sectional study included 4004 participants of a sub-population of the Kailuan Study. All the study participants underwent structured interviews with a standardized questionnaire, fundus photography and spectral-domain optical coherence tomography (OCT) examinations performed by trained doctors. RESULTS: The retinal nerve fiber layer thickness was significantly higher (P=0.006) and the central macular thickness was lower in coal miners (n=659, 51.0±7.8y) as compared to the control (working above the ground; n=477, 51.8±7.5y; P=0.032). Additionally, the downhole workers showed a significantly thicker retinal artery (P=0.012) and vein diameters (P<0.001). In multivariable regression, a thicker retinal nerve fiber layer was associated with a higher cumulative silica dust exposure (P=0.005) after adjusting for younger age and larger spherical equivalent. In a reverse pattern, a higher cumulative silica dust exposure (P=0.004) was significantly associated with a thicker retinal nerve fiber layer after adjusting for age, high-density lipoproteins and uric acid. Wider retinal vein diameters were associated with higher cumulative silica dust exposure (P=0.036) after adjusting for younger age and larger spherical equivalent. CONCLUSION: The retinal vessels diameters and retinal nerve fiber layer thickness are significantly thicker in long term of coal mining. The results of our study indicate that underground working environment may lead to retinal vessel dilation and inflammation. Thus, ocular examination might be needed within coal miners in order to monitor the occupational eye health as well as the incidence and progression of eye diseases.

Quartz Mining Waste for Concrete Production: Environment and Public Health

Brazil, one of the largest ore producers in the world, holds 95% of the world’s quartz reserves. The aim of this research is to enhance mitigation measures in quartz ore exploration common in the Serra do Espinhaço Meridional region through the specific study of a mining venture. The three (3) phases of the study were: (1) characterization of the project under study and evaluation of the impacts generated; (2) evaluation of the economic feasibility of using the waste as a coarse aggregate for concrete production; (3) evaluation of the technical feasibility of reusing this waste through analysis of compressive strength. The results of the study show potential negative impacts on occupational health from the piles of disposed waste, specifically silicosis, caused by silica dust dispersed in the air. In the economic analysis, a decrease of 49.05% was verified in coarse aggregate cost through the use of the residue. The compressive strength of the concrete was 26.80 MPa when quartz residue was used and 29.2 MPa when limestone was used. The quartz residue generated by the venture can be reused as aggregate for the production of concrete, generating improvements in environmental and health aspects.

A rapid point of care CC16 kit for screening of occupational silica dust exposed workers for early detection of silicosis/silico-tuberculosis

Silicosis is an irreversible, incurable and progressive occupational disease caused by prolonged exposure to crystalline-silica dust while working in the relevant industries. Conventionally diagnosis is done by chest radiology, often in an advanced stage as early symptoms often go unnoticed. Early detection and necessary intervention (secondary prevention) could be a realistic possible control strategy for controlling silicosis as no effective treatment is available to stop and/or reverse the pathological process. Additionally, these patients are also vulnerable to pulmonary tuberculosis, which often becomes difficult to treat and with uncertain treatment outcome. Considering India has a huge burden of silicosis and silico-tuberculosis, a rapid and inexpensive screening method was realized to be an urgent need for early detection of silicosis among silica dust exposed workers. Serum club cell protein 16 (CC16) is evidenced to be a useful proxy screening marker for early detection of silicosis as evidenced from the recent research work of ICMR-National Institute of Occupational Health (ICMR-NIOH), India. In this study a lateral-flow assay for semi-quantitative estimation of serum CC16 level was developed. The detection was performed using gold nanoparticles conjugated anti-CC16 monoclonal antibodies. A sum of 106 serum samples was tested to do the performance evaluation of the assay. A concentration of 6 ng/ml or less produced one band, 6.1–9 ng/ml produced two bands, while more than 9 ng/ml produced all the three bands at the test zone. The sensitivity of the assay was 100% while the specificity was 95%. This assay may be used as a sensitive tool for periodic screening of silica dust exposed vulnerable workers for early detection of silicosis in them.

CC-Chemokine Ligand 18, CXC Motif Chemokine 13, and Osteopontin as Biomarkers of Silicosis and Asbestosis: A Case-Control Study

Background Silicosis and asbestosis are progressive pneumoconiosis characterized by interstitial fibrosis exposure to silica dust or asbestos fibres. This study aimed to explore the potential biomarkers for diagnosis of silicosis or asbestosis. Methods Plasma concentrations of CC-chemokine ligand 18 (CCL18), CXC motif chemokine 13 (CXCL13), osteopontin (OPN), periostin, and fibulin-3 were compared between silicosis, asbestosis, and healthy controls (HCs). The receiver operating characteristic (ROC) curve was used to monitor the diagnostic sensitivity, and their correlation to disease severity was examined. Results Plasma CCL18, CXCL13, and OPN concentrations in both patient groups were high. For silicosis patients, these plasma concentrations were significantly correlated with predicted forced vital capacity (FVC%) and lung diffusing capacity for carbon monoxide (DLCO SB%). The order of diagnostic accuracy was CCL18, OPN, and CXCL13 in silicosis or asbestosis. Combining CCL18, OPN, and CXCL13 can improve diagnostic accuracy. Conclusions CCL18, CXCL13, and OPN are available biomarkers for the diagnosis of silicosis and asbestosis.

Masonry Dust Risk Control Practices and the Barriers to the Adoption of Engineering Controls in Reducing Risk due to Dust Exposure in Masonry Work

The construction industry is known to be among the most vulnerable industries in terms of occupational safety and health. One of the riskiest activities in construction involves masonry, which reports several cases involving occupational health diseases due to the exposure to silica dust. Thus, this study aims to investigate the perception toward health risks due to exposure to dust in masonry work. A survey research method was adopted for this study, which involved the participation of 25 active construction contractors in Selangor and Kuala Lumpur. The findings reveal that respiratory problems and health diseases are the most perceived risks associated to dust exposure in masonry work. There are various barriers to the adoption of engineering control in masonry work such as high costs, lack of awareness of dust hazards, and workers’ attitudes. The current practical intervention methods used by contractor firms are respiratory protection, wet method, and sweeping compound. This study provides information on the current masonry work environment and the barriers of the adoption of engineering control technologies. Additionally, this study suggests that the key players in the construction industry should take an active part to increase the implementation of engineering controls in a construction project, and not rely solely on the use of PPE.

Tuberculosis and Silicosis Burden in Artisanal and Small-Scale Gold Miners in a Large Occupational Health Outreach Programme in Zimbabwe

Artisanal and small-scale miners (ASMs) labour under archaic working conditions and are exposed to high levels of silica dust. Exposure to silica dust has been associated with an increased risk of tuberculosis and silicosis. ASMs are highly mobile and operate in remote areas with near absent access to health services. The main purpose of this study was to evaluate the prevalence of tuberculosis, silicosis and silico-tuberculosis among ASMs in Zimbabwe. A cross-sectional study was conducted from 1 October to 31 January 2021 on a convenient sample of 514 self-selected ASMs. We report the results from among those ASMs who attended an outreach medical facility and an occupational health clinic. Data were collected from clinical records using a precoded data proforma. Data variables included demographic (age, sex), clinical details (HIV status, GeneXpert results, outcomes of chest radiographs, history of tuberculosis) and perceived exposure to mine dust. Of the 464 miners screened for silicosis, 52 (11.2%) were diagnosed with silicosis, while 17 (4.0%) of 422 ASMs were diagnosed with tuberculosis (TB). Of the 373 ASMs tested for HIV, 90 (23.5%) were sero-positive. An HIV infection was associated with a diagnosis of silicosis. There is need for a comprehensive occupational health service package, including TB and silicosis surveillance, for ASMs in Zimbabwe. These are preliminary and limited findings, needing confirmation by more comprehensive studies.

3-methyadenine inhibits lipopolysaccharides-induced pulmonary inflammation at the early stage of silicosis via blocking NF-κB signaling pathway

Occupational exposure to silica dust is related to pulmonary inflammation and silicosis. Lipopolysaccharides (LPSs) could aggravate apoptosis in alveolar macrophages (AMs) of human silicosis through autophagy, yet how the reduction of autophagy attenuated LPS-induced lung injury and the related mechanisms need to be investigated. In the study, we aim to understand the role of 3-methyladenine (3-MA), an inhibitor of autophagy, in LPS-mediated inflammatory responses and fibrosis. We collected AMs from observers/silicosis patients. The results showed that LPS induced NF-κB-related pulmonary inflammation in observers and silicosis patients, as confirmed by an increase in the expression of IL-1β, IL-6, TNF-α, and p65, which could be inhibited by 3-MA treatment. In mice models, at the early stage (7d) of silicosis, but not the late (28d) stage, blocking autophagy reversed the increased levels of IL-1β, IL-6, TNF-α, and p65 caused by LPS. Mechanism study revealed that LPS triggered the expression of LC3 II, p62, and cleaved caspase-3 at the early stage exposed to silica, which could be restored by 3-MA, while there was no difference in the expression of LAMP1 either at the early or late stage of silicosis in different groups. Similarly, 3-MA treatment did not prevent fibrosis characterized by destroyed alveoli, collagen deposition, and increased expression of α-SMA and Col-1 induced by LPS at the late stage of silicosis. The results suggested that 3-MA has a role in the protection of lung injury at the early stage of silicosis and provided an experimental basis for preventive strategies of pulmonary inflammation and silicosis.