This study investigates the practical predictability of two simulated mesoscale convective systems (MCS1 and MCS2) within a state-of-the-art convection-allowing ensemble forecast system. The two MCSs are both controlled by the synoptic Meiyu-front but differ in mesoscale orographic forcing. An observation system simulation experiment (OSSE) setup is first built, which includes flow-dependent multiple-scale initial and lateral boundary perturbations and a 12 h 30-member ensemble forecast is thereby created. In combination with the difference total energy, the decorrelation scale and the ensemble sensitivity analysis, both forecast error evolution, precipitation uncertainties and meteorological sensitivity that describe the practical predictability are assessed. The results show large variabilities of precipitation forecasts among ensemble members, indicative of the practical predictability limit. The study of forecast error evolution shows that the error energy in the MCS1 region in which the convection is blocked by the Dabie Mountains exhibits a simultaneous peak pattern for all spatial scales at around 6 h due to strong moist convection. On the other hand, when large-scale flow plays a more important role, the forecast error energy in the MCS2 region exhibits a stepwise increase with increasing spatial scale. As a result of error energy growth, the precipitation uncertainties evolve from small scales and gradually transfer to larger scales, implying a strong relationship between error growth and precipitation across spatial scales, thus explaining the great precipitation variability within ensemble members. These results suggest the additional forcing brought by the Dabie Mountains could regulate the predictability of Meiyu-frontal convection, which calls for a targeted perturbation design in convection-allowing ensemble forecast systems with respect to different forcing mechanisms.