Plant Gravitropism and Signal Conversion under a Stress Environment of Altered Gravity

Humans have been committed to space exploration and to find the next planet suitable for human survival. The construction of an ecosystem that adapts to the long-term survival of human beings in space stations or other planets would be the first step. The space plant cultivation system is the key component of an ecosystem, which will produce food, fiber, edible oil and oxygen for future space inhabitants. Many plant experiments have been carried out under a stimulated or real environment of altered gravity, including at microgravity (0 g), Moon gravity (0.17 g) and Mars gravity (0.38 g). How plants sense gravity and change under stress environment of altered gravity were summarized in this review. However, many challenges remain regarding human missions to the Moon or Mars. Our group conducted the first plant experiment under real Moon gravity (0.17 g) in 2019. One of the cotton seeds successfully germinated and produced a green seedling, which represents the first green leaf produced by mankind on the Moon.

Epigenomic Regulators Elongator Complex Subunit 2 and Methyltransferase 1 Differentially Condition the Spaceflight Response in Arabidopsis

Background: Plants subjected to the novel environment of spaceflight show transcriptomic changes that resemble aspects of several terrestrial abiotic stress responses. Under investigation here is whether epigenetic modulations, similar to those that occur in terrestrial stress responses, have a functional role in spaceflight physiological adaptation. The Advanced Plant Experiment-04 – Epigenetic Expression experiment examined the role of cytosine methylation in spaceflight adaptation. The experiment was conducted onboard the International Space Station, and evaluated the spaceflight-altered, genome-wide methylation profiles of two methylation-regulating gene mutants [methyltransferase 1 (met1-7) and elongator complex subunit 2 (elp2-5)] along with a wild-type Col-0 control.Results: The elp2-5 plants suffered in their physiological adaptation to spaceflight in that their roots failed to extend away from the seed and the overall development of the plants was greatly impaired in space. The met1-7 plants suffered less, with their morphology affected by spaceflight in a manner similar to that of the Col-0 controls. The differentially expressed genes (DEGs) in spaceflight were dramatically different in the elp2-5 and met1-7 plants compared to Col-0, indicating that the disruptions in these mutants resulted in a reprogramming of their spaceflight responses, especially in elp2-5. Many of the genes comprising the spaceflight transcriptome of each genotype were differentially methylated in spaceflight. In Col-0 the majority of the DEGs were representative of the now familiar spaceflight response, which includes genes associated with cell wall remodeling, pathogen responses and ROS signaling. However, the spaceflight transcriptomes of met1-7 and elp2-5 each presented patterns of DEGs that are almost completely different than Col-0, and to each other. Further, the DEGs of the mutant genotypes suggest a more severe spaceflight stress response in the mutants, particularly in elp2-5.Conclusion: Arabidopsis physiological adaptation to spaceflight results in differential DNA methylation in an organ-specific manner. Disruption of Met1 methyltransferase function does not dramatically affect spaceflight growth or morphology, yet met1-7 reprograms the spaceflight transcriptomic response in a unique manner. Disruption of elp2-5 results in poor development in spaceflight grown plants, together with a diminished, dramatically reprogrammed transcriptomic response.

Effect of Reaction Time and Hydrothermal Treatment Time on the Textural Properties of SBA-15 Synthesized Using Sodium Silicate as a Silica Source and Its Efficiency for Reducing Tobacco Smoke Toxicity

The synthesis of SBA-15 has been optimized using sodium silicate, an inexpensive precursor of SBA-15. In this work, the influence of synthesis times of the precipitation and the hydrothermal treatment steps, on the textural properties developed as well as for reducing the toxic compounds generated in tobacco smoking, has been studied. The hydrothermal treatment has been proved to be necessary to obtain materials with adequate performance in this particular application. Twenty-four hours of hydrothermal treatment provide materials with the best properties. Although the reaction stage usually involves the mixing of reagents during 24 h, 40 min is enough to obtain a material with stick-like morphology and typical textural properties. Moreover, between 1 and 2 h of reaction time, the material proved to have the best performance for the purpose of reducing the toxicity of the products generated during the tobacco smoking process. These results are of great significance for an eventual scaling up to industrial scale of the SBA-15 manufacturing process. Results of a pilot plant experiment in a batch of 4 kg of SBA-15 are reported.

Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions

The development of rhizobial inoculants with increased resistance to abiotic stress is critical to mitigating the challenges related to climate change. This study aims at developing a soybean stress-tolerant Bradyrhizobium inoculant to be used under the mixed stress conditions of acidity, high temperature, and drought. Six isolates of Bradyrhizobium with high symbiotic performance on soybean were tested to determine their growth or survival abilities under in vitro conditions. The representative stress-tolerant Bradyrhizobium isolates 184, 188, and 194 were selected to test their ability to promote soybean growth under stress conditions compared to the type strain Bradyrhizobium diazoefficiens USDA110. The plant experiment indicated that isolate 194 performed better in symbiosis with soybean than other Bradyrhizobium strains under stress conditions. Based on the stress tolerance index, soybeans inoculated with isolate 194 showed a high growth performance and significantly better nodulation competition ability than USDA110 under several stress conditions. Interestingly, supplementation of sucrose in the culture medium significantly enhances the survival of the isolate and leads to improved plant biomass under various stress conditions. Analysis of the intra-cellular sugars of isolate 194 supplemented with sucrose showed the accumulation of compatible solutes, such as trehalose and glycerol, that may act as osmoprotectants. This study indicates that inoculation of stress-tolerant Bradyrhizobium together with sucrose supplementation in a medium could enhance bacterial survival and symbiosis efficiency under stress conditions. Although it can be applied for inoculant production, this strategy requires validation of its performance in field conditions before adopting this technology.

Combustion Melting Characterisation of Solid Fuel Obtained from Sewage Sludge

Solid fuelization technology can increase the heating value of sewage sludge such that it can be utilised as a fossil fuel substitutes. Reducing landfilling of bottom and fly ash resulting from heavy metals contained in sewage sludge is challenging. Hence, combustion melting technology (CMT), which can discharge bottom ash in the form of slag, has been proposed herein as an alternative to the conventional incineration technology. However, further research is required to improve the flowability of slag. Applicability of CMT for the stable treatment of heavy metals in the ash generated during the energisation of sewage sludge solid fuel has been reviewed. The change in the degree of fluidity was identified via a laboratory-scale fluidity measurement experiment following changes in melting temperature, mixing ratio of sewage sludge and sawdust, and basicity. The pouring index (PI) of sewage sludge solid fuel (pellet) was maintained at a level of about 60% at a basicity index of 0.8. Based on the results, the slagging rates and volume reduction rates, exhaust gas analysis, and heavy metal elution characteristics under oxygen enrichment were derived from a 2 ton/day combustion melting pilot plant experiment; thereafter, the feasibility of combustion melting of sewage sludge solid fuel was determined.

Macroinvertebrate Recovery to Varying Hydropeaking Frequency: A Small Hydropower Plant Experiment

As the demand for hydroelectricity progresses worldwide, small hydropower operators are increasingly examining the feasibility of using existing infrastructure (e.g., settling basins) in run-of-the-river schemes for intermittent power production. Such flexible production causes short-term discharge fluctuations (hydropeaking) in downstream reaches with potential adverse effects for the sensitive fauna and flora in alpine streams. In an experimental field study on a previously unregulated section of the upper Rhone River (Switzerland), we measured density and composition of macroinvertebrate drift in two habitats (riffle, pool) following a 15-minute hydropeaking wave. The experimental hydropeaking was replicated five times over 14 days with decreasing recovery times between peaks (8, 3, 2 days, and 24 h), and drift measurements were compared with kick samples for the benthic community. Results from the kick sampling showed that benthic macroinvertebrate abundance and composition did not significantly change between the experimental peaks. There were habitat specific reactions in macroinvertebrate drift to hydropeaking, with the pool experiencing more pronounced drift abundances than the riffle. Overall, drift abundance was not significantly correlated with recovery time, but results indicate taxa-specific differences. This research advocates for the importance of completing more in-situ field experiments in order to better understand the ecological impact of flexible power production in small hydropower plants.

Fear of contamination and public opinion on nuclear energy

An increasing number of experts agree that nuclear power should be part of the solution to fight climate change as it emits little greenhouse gases, has had no negative health consequences during normal operation, and even limited consequences after accidents. However, in many countries the population is much more ambivalent about nuclear power, and tends to exaggerate the negative effects on health and the environment. We suggest that this gap between experts and the public stems in part from nuclear power triggering the behavioral immune system: a set of cognitive adaptations that aim at protecting us against pathogens by making us particularly alert to their existence, and attuned to their risks. In line with this suggestion, we find that (i) participants overestimate the risks of nuclear accidents compared to other types of disasters (Experiment 1), except for disasters that should also trigger the behavioral immune system (Experiment 2); (ii) participants were more interested in reading and sharing a news article about a nuclear accident than about other types of accidents (with the same exception, Experiment 2); (iii) participants were less willing to be in contact with an object that had been in a nuclear power plant than in a car manufacturing plant (Experiment 3); (iv) arguments showing that nuclear power plants should not elicit fears of contamination reduced the negative perception of nuclear energy (Experiment 4). This work suggests a cognitive basis for the popular rejection of nuclear power, and ways to bridge the gap between experts and the public on this topic.

Effects of Growth Substrate and Container Size on Cutting Production from Mojave Sage Stock Plants

Stock plant productivity is an important concern for growers of mojave sage (Salvia pachyphylla) because this species produces more woody growth as the plant ages. The objective of the study was to determine the best growth substrate and container size combination to maximize stock plant productivity. A secondary objective was to determine whether the stock plant treatments influenced the rooting of vegetative cuttings. Three different container sizes (3, 12, and 15.5 qt) and four soilless substrates composed primarily of bark, peat, and perlite (substrate 1); bark, peat, and vermiculite (substrate 2); bark, peat, and coarse perlite (substrate 3); and peat (substrate 4) were used. The stock plant experiment was conducted using 12 treatment combinations, and a subset of those stock plants was selected randomly for the rooting study that immediately followed the stock plant experiment. Stock plants responded to substrate treatments differently. The most successful stock plants, which produced more cuttings per plant and per square foot, as well as larger cuttings, were those grown in substrate 3. Regardless of substrate, the highest number of cuttings per square foot was obtained from stock plants grown in 3-qt containers, indicating that the smaller containers allow for the most efficient use of space when growing mojave sage stock plants for 4 to 6 months. The rooting of vegetative cuttings was successful (88% to 100% of cuttings rooted after 4 weeks under mist) for all treatment combinations.

Impacts of Growth Substrate and Container Size on Cutting Production from ‘Snow Angel’ Coral Bells Stock Plants

Stock plant productivity is an important concern for growers of ‘Snow Angel’ coral bells (Heuchera sanguinea) because this variety produces a limited number of basal cuttings. The objective of the study was to determine the best growth substrate and container size combination to maximize productivity of stock plants. A secondary objective was to determine if the stock plant treatments influenced the rooting of vegetative cuttings. The study used three different container sizes (2.8, 11.4, and 14.6 L) and four commercial soilless substrates that were primarily composed of the following: bark, peat, and perlite (substrate 1); bark, peat, and vermiculite (substrate 2); bark, peat, and coarse perlite (substrate 3); and peat (substrate 4). Two stock plant experiments were conducted using the same 12 treatment combinations, and a subset of those stock plants was randomly selected for the rooting studies that immediately followed each stock plant experiment. Stock plants responded to substrate treatments differently depending on the batch of substrate in which they were grown. The most successful stock plants, which produced more cuttings per plant and per square foot, as well as larger cuttings, were those grown in substrate 3 (Expt. 1) and substrate 2 (Expt. 2). Regardless of the substrate, the highest number of cuttings per square foot was obtained from stock plants grown in 2.8-L containers, indicating that the smaller containers allow for the most efficient use of space when growing ‘Snow Angel’ stock plants for 6 to 8 months. The rooting of vegetative cuttings was successful (98% to 100% of cuttings rooted after 4 weeks under mist) for all treatment combinations, although higher numbers of visible roots were produced during the second study and may be due to larger fresh weights of cuttings.