Arabidopsis thaliana endonuclease V is a ribonuclease specific for inosine-containing single-stranded RNA

Endonuclease V is highly conserved, both structurally and functionally, from bacteria to humans, and it cleaves the deoxyinosine-containing double-stranded DNA in Escherichia coli , whereas in Homo sapiens it catalyses the inosine-containing single-stranded RNA. Thus, deoxyinosine and inosine are unexpectedly produced by the deamination reactions of adenine in DNA and RNA, respectively. Moreover, adenosine-to-inosine (A-to-I) RNA editing is carried out by adenosine deaminase acting on dsRNA (ADARs). We focused on Arabidopsis thaliana endonuclease V (AtEndoV) activity exhibiting variations in DNA or RNA substrate specificities. Since no ADAR was observed for A-to-I editing in A. thaliana , the possibility of inosine generation by A-to-I editing can be ruled out. Purified AtEndoV protein cleaved the second and third phosphodiester bonds, 3′ to inosine in single-strand RNA, at a low reaction temperature of 20–25°C, whereas the AtEndoV (Y100A) protein bearing a mutation in substrate recognition sites did not cleave these bonds. Furthermore, AtEndoV, similar to human EndoV, prefers RNA substrates over DNA substrates, and it could not cleave the inosine-containing double-stranded RNA. Thus, we propose the possibility that AtEndoV functions as an RNA substrate containing inosine induced by RNA damage, and not by A-to-I RNA editing in vivo .

Pd/Alumina Catalysts for Beneficial Transformation of Harmful Freon R-22

Chlorodifluoromethane (R-22), the most abundant freon in the atmosphere, was subjected to successful hydrodechlorination in the presence of palladium supported on γ-alumina, at a relatively low reaction temperature (180 °C). The combination of catalytic actions of alumina (performing freon dismutation) and Pd nanoparticles (catalyzing C–Cl bond splitting in the presence of hydrogen) results in the transformation of freon into valuable, chlorine-free products: methane and fluoroform, the mixture of which is used in plasma etching of silicon and silicon nitride. Very highly metal dispersed Pt/Al2O3 catalysts, with metal particles of ~1.3 nm in size, are not as effective as Pd/Al2O3, resulting in only partial dechlorination. A long-term dechlorination screening (3–4 days) showed good catalytic stability of Pd/alumina catalysts.

Long-Term Changes in Floristic Diversity as an Effect of Transforming the Lake into a Retention Reservoir

The Łęczna-Włodawa Lake District is one of the most valuable natural regions in Europe. It is an area of numerous lakes, peat bogs, swamps and forests, which has been undergoing intensive transformation for decades. Among the largest projects were the creation of the Wieprz Krzna Canal system along with the drainage system and the transformation of natural lakes into retention reservoirs. Among the transformed lakes is Lake Wytyckie. The land was used for analyses near the lake, and floristic and habitat analyses were carried out within the boundaries of the contemporary embankment. The studies were carried out from the 1950s, when the lake functioned as a natural reservoir, through to the 1980s (the transformation of the lake), to the 2020s. Lake Wytyckie was transformed into a retention reservoir by increasing its size and flooding the areas inhabited mainly by peat bog, meadow and forest vegetation, which contributed to the impoverishment of both species and habitat diversity of the area, while it increased the nutrient richness of the water. This was reflected both in the decline in the value of individual diversity indices as well as in the ecological index numbers. In the first period of the research, the area was dominated by wetlands, not drained, with a large variety of species that preferred good lighting conditions. Additionally, the habitat was characterized by low reaction, temperature and trophic values. In the following period, there was an increase in the depth of the water of the reservoir, characterized by high water visibility values, which contributed to the presence of protected species, as did the low moisture content of the areas within the embankment and a neutral pH. The factors currently influencing the formation of the vegetation structure are the high humidity of the entire embankment area, the increase in pH, and the significant increase in the share of built-up areas in the immediate vicinity.

Synthesis of CuInS2/ZnS Nanocrystals with Thick Shell: The Effect of Temperature and Sulfur Precursor

As heavy-metal-free alternatives to Cd- and Pb-containing semiconductor nanocrystals (NCs), ternary I–III–VI2 compounds NCs have been actively studied, especially the CuInS2 NCs. Recently, it has been found that thick ZnS shelling can greatly improve the photochemical stability of such NCs, which undoubtedly enhances their application potential although it is still limited by the development of synthetic methods. This paper provides a facile method for preparation of thick-shell CuInS2/ZnS NCs. The effects of reaction temperatures ([Formula: see text]C and [Formula: see text]C) and sulfur precursors (dodecanethiol and sulfur powder) on the shell overgrowth are discussed in detail. When low reaction temperature ([Formula: see text]C) and inactive sulfur precursor are used, the overgrowth of ZnS shell is considerably slow and raising temperature have a limited impact on the particles’ size. On the contrary, high reaction temperature and reactive sulfur precursor can effectively improve the overgrowth rate of ZnS shell, and then thick-shell CuInS2/ZnS NCs can be received. Furthermore, a high-speed centrifugation method is used to screen out product NCs with a relatively uniform size.

The Effect of Si on CO2 Methanation over Ni-xSi/ZrO2 Catalysts at Low Temperature

A series of Ni-xSi/ZrO2 (x = 0, 0.1, 0.5, 1 wt%, the controlled contents of Si) catalysts with a controlled nickel content of 10 wt% were prepared by the co-impregnation method with ZrO2 as support and Si as a promoter. The effect of different amounts of Si on the catalytic performance was investigated for CO2 methanation with the stoichiometric H2/CO2 molar ratio (4/1). The catalysts were characterized by BET, XRF, H2-TPR, H2-TPD, H2-chemisorption, CO2-TPD, XRD, TEM, XPS, and TG-DSC. It was found that adding the appropriate amount of Si could improve the catalytic performance of Ni/ZrO2 catalyst at a low reaction temperature (250 °C). Among all the catalysts studied, the Ni-0.1Si/ZrO2 catalyst showed the highest catalytic activity, with H2 and CO2 conversion of 73.4% and 72.5%, respectively and the yield of CH4 was 72.2%. Meanwhile, the catalyst showed high stability and no deactivation within a 10 h test. Adding the appropriate amount of Si could enhance the interaction between Ni and ZrO2, and increase the Ni dispersion, the amounts of active sites including surface Ni0, oxygen vacancies, and strong basic sites on the catalyst surface. These might be the reasons for the high activity and selectivity of the Ni-0.1Si/ZrO2 catalyst.

Hydrocracking of C5-Isolated Asphaltene and Its Fractions in Batch and Semi-Batch Reactors

Non-catalytic and catalytic hydrocracking of C5-isolated asphaltene and its subfractions were performed in batch and semi-batch reactors at various temperatures. Catalyst and H2 played an important role in the hydrocracking of asphaltenes. In the batch system, the catalyst enhanced asphaltene conversion to light liquid products and suppressed coke formation. The coke formation was controlled at a low reaction temperature, but the reaction rate was too low. Light liquid products were also formed at the beginning of the reaction even at high temperatures, but the coke formation was predominant as the reaction time went on due to the decrease in H2 amount in the reactor. To solve these problems, H2 was continuously supplied during the reaction using the semi-batch system. Sufficient supply of H2 improved the conversion of asphaltenes to light liquid products while inhibiting the coke formation. The lightest asphaltene fraction was easily cracked into light products by inhibiting the coke formation, while the heaviest fraction tends to form coke. The lightest asphaltene fraction prolonged the coke induction period of the heaviest fraction during the catalytic hydrocracking because the lightest fraction contained a significant amount of heavy resin close to that which could prevent aggregation of the heaviest asphaltenes.

Synthesis and Characterization of Pure and Magnesium (Mg) Doped CuO Nano Particles by Solid State Method

Pure and Magnesium (Mg) doped CuO nanoparticles were effectively prepared by solid state method using Copper Chloride (CuCl2 · 2H2O), Magnesium Chloride (MgCl2 · 6H2O) and Sodium Hydroxide (NaOH). The ITO/TiO2/CuO–M/Cu heterojunctions solutions were fabricated by Dr Blade method at a low reaction temperature of 400 °C. In this analysis the prepared nanostructure were exposed to structural, morphological and optical analysis. The X-ray diffraction peaks results divulged that the prepared nanoparticles are monoclinic structure of CuO which was evidenced from the JCPDS card No. 801916. Morphological analysis result exposed that pure CuO particles exclusively consist of agglomerated irregular spherical shape. The result of the elemental analysis confirmed the presence of Mg2+ in the doped samples. Fourier Transform Infra-Red Spectroscopy results reveals the structural modifications due to the presence of magnesium. Optical absorption measurement spectra demonstrates that band gap shift is due the proportion of Mg.