scholarly journals FEATURES OF THE KARYOTYPE OF WHITE-HEADED COWS OF UKRAINIAN BREED

2020 ◽  
pp. 177-185
Author(s):  
Lubov Starodub
Keyword(s):  
Bos Taurus ◽  
Micronucleus Test ◽  
Chromosome Abnormalities ◽  
Mean Values ◽  
Average Value ◽  
Chromosomal Breaks ◽  
Dividing Cells ◽  
Cytogenetic Monitoring ◽  
The Difference ◽  
Dairy Breed

The White-Headed Ukrainian breed of cattle belongs to breeds of a dairy direction of productivity and belongs to small disappearing breeds. According to calculations, in accordance with the FAO recommendations, the White-Headed Ukrainian breed of cattle is at risk. The aim of the work was to establish the karyotype variability of White-Headed Ukrainian cows, whose animals belong to closed gene pool herds. Cytogenetic monitoring showed that the quantitative chromosome abnormalities, in particular aneuploidy, in White-Headed Ukrainian cows of two groups according to the purpose of the cow (selection nucleus and production group) did not exceed the spontaneous level of this variability (1.5-8.3%) and was 6.0% and 7.0% respectively. A multiple increase in the number of chromosomes (polyploidy) was not detected. The average value of metaphase plates with asynchronous differences in the centromeric regions of chromosomes was 1.0% and corresponded to the level of this variability characteristic of the species Bos taurus. The frequency of structural chromosome abnormalities (chromosomal breaks) in animals of the two groups was 2.3% and 2.0%, respectively, which corresponds to the norm characteristic of cattle with spontaneous mutagenesis. The results of the micronucleus test showed that the proportion of lymphocytes with micronuclei in animals of the two groups was in the range of 4.8 - 5.0%, dinuclear lymphocytes 5.6-6.4 %, and the proportion of dividing cells (mitotic index) was 2.8-3.8 %. The frequency of cells with aneuploidy in cows of the Ukrainian Black-Spotted dairy breed of this farm was established higher than the spontaneous level. The difference between the mean values on this basis in cows of White-Headed Ukrainian and Ukrainian Black-Spotted dairy breeds was statistically significant at P> 0.99. In cows of the Ukrainian Black-Spotted dairy breed under conditions of intensive use, an increase in the frequency of metaphases with aneuploidy was observed, which was not manifested in cows of the White-Headed Ukrainian breed.

scholarly journals Investigation of the effects of some bacterial isolates and fertilizer applications on nutrient contents of hyacinth

2020 ◽  
Vol 48 (2) ◽  
pp. 978-988
Author(s):  
Pınar A. BINTAS ◽  
Arzu ÇIĞ ◽  
Nalan TÜRKOĞLU
Keyword(s):  
Bacterial Species ◽  
Nitrogen Fixing ◽  
Mean Values ◽  
Nutrient Contents ◽  
Average Value ◽  
Cu Content ◽  
Hyacinthus Orientalis ◽  
The Difference ◽  
Mg Content ◽  
Npk Fertilization

This study was conducted to determine the effect of NPK fertilization and some bacterial species on the macro and micro nutrient element contents of Hyacinthus orientalis cv. ‘Delft Blue’ leaves and bulbs. In the study carried out under laboratory conditions, according to randomized plot experiment design, full and 50% dose of 20:20:20 NPK commercial fertilizer was applied to hyacinth bulbs as inorganic fertilizer. Nitrogen-fixing bacteria: Cellulomonas turbata (TV54A), phosphate solibilizing bacteria: Bacillus-GC Group (TV119E), nitrogen-fixing and phosphate solibilizing bacteria: Kluyvera cryocrescens (TV113C)were used as biological fertilizers. In this study, the highest mean values of nutrients were obtained as nitrogen (N) 3.866%; phosphorus (P) 334.790 mgkg-1;potassium (K) 4.534%; iron (Fe) 152.453 mgkg-1; manganese (Mn) 26.739 mgkg-1; zinc (Zn) 46.517 mgkg-1and copper (Cu) 8.902 mgkg-1 (P <0.01) in hyacinth leaves, while calcium (Ca) was 1064.95 mgkg-1 and magnesium (Mg) was found 1992.92 mgkg-1, and the difference between the mean values for these two elements was not statistically significant.  The highest N, P, Zn and Cu contents of the leaf were obtained by using full dose NPK. The highest mean values of nutrient elements in hyacinth bulbs were 4.382%; P 278.496 mgkg-1; K, 2.203%; Ca 2322.045 mgkg-1; Mg 1884.164 mgkg-1; Mn 39.640 mgkg-1; Zn was obtained as 40.861 mgkg-1 and Cu 7.683 mgkg-1, whereas Fe was found to have an average value of 140.545 mgkg-1. In the process of bacteria inoculation K, Ca and Mg content of inoculation; N, P, Mn, Zn and Cu content of ½ NPK fertilization was determined to be effective.

scholarly journals Diagnostic Potential of Calprotectin for Spontaneous Bacterial Peritonitis in Patients Withliver Cirrhosis and Ascites

PRILOZI ◽  
2021 ◽  
Vol 42 (3) ◽  
pp. 97-106
Author(s):  
Fana Lichoska Josifovikj ◽  
Kalina Grivcheva Stardelova ◽  
Beti Todorovska ◽  
Magdalena Genadieva Dimitrova ◽  
Nenad Joksimovikj ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Antibiotic Treatment ◽  
Spontaneous Bacterial Peritonitis ◽  
Mean Value ◽  
Mean Values ◽  
Average Value ◽  
Bacterial Peritonitis ◽  
Diagnostic Potential ◽  
The Mean ◽  
The Difference

Abstract The development of spontaneous bacterial peritonitis (SBP) is a serious and life-threatening condition in patients with cirrhosis and ascites. The aim of this study was to determine the diagnostic potential of calprotectin in ascites, for SBP in patients with liver cirrhosis and ascites before and after antibiotic treatment and to compare the mean values of calprotectin in ascites in patients with and without SBP. This prospective-observational study was comprised of 70 patients with cirrhosis and ascites, divided into two groups, the SBP and the non-SBP group. Quantitative measurements of calprotectin in ascites was completed with the Quantum Blue Calprotectin Ascites test (LF-ASC25), using the Quantum Blue Reader. The average value of calprotectin in the SBP group was 1.5 ± 0.40 μg / mL, and in the non-SBP group it was lower (0.4 ± 0.30). The difference between the mean values was statistically significant with p <0.05. The mean value of calprotectin in ascites before therapy among the SBP group was 1.5 ± 0.4, and after antibiotic therapy, the value decreased significantly to 1.0 ± 0.6; the difference between the mean values was statistically significant with p <0.05. ROC analysis indicated that calprotectin contributed to the diagnosis of SBP with a 94.3% sensitivity rating (to correctly identify positives), and the specificity was 62.5%, which corresponded to the value of 0.275. Our research confirmed that ascitic calprotectin was a good predictor, and is significantly associated with the occurrence of SBP in patients with liver cirrhosis. By monitoring the value of calprotectin in ascites on the 7th day of antibiotic treatment, the effectiveness of antibiotic treatment in patients with SBP can be determined.

scholarly journals The influence of different working lengths on apically extruded debris

2021 ◽  
Vol 10 (4) ◽  
pp. e55410414554
Author(s):  
Karla Garcia ◽  
Ana Grasiela da Silva Limoeiro ◽  
Wayne Martins Nascimento ◽  
Eduardo Mansur Kadi ◽  
Sandra Radaic ◽  
...  
Keyword(s):  
Average Weight ◽  
Agar Gel ◽  
Mean Values ◽  
Root Canals ◽  
Average Value ◽  
Working Length ◽  
Coronal Section ◽  
The Difference ◽  
The One ◽  
Post Hoc

Aim: To evaluate the influence of three different working lengths on the amount of apically extruded debris. Methodology: Thirty lower premolars with single roots and straight root canals were standardized at 17 mm. Then, they were inserted into Eppendorf tubes, and the 1.5% agar gel was introduced into the tubes surrounding the roots. The coronal section of the roots was kept visible. The set of tubes and agar gel was weighed 3 times and the average value was recorded. Then, the specimens were randomly distributed in 3 different groups according to the working length (CT) used for the instrumentation: Group (CT -1) - the working length 1 mm below the major foramen (MF); Group (CT 00) - the length was determined at the MF, and Group (CT +1) - the CT was determined 1 mm beyond the MF. Instrumentation was performed with Reciproc Blue R25 (VDW, Munich, Germany) under irrigation with 0.9% saline. After preparation, samples were removed from Eppendorf tubes and weighed 3 times again. The difference between the mean values ​​of the initial and final weight was recorded. The one-way ANOVA test (post-hoc Bonferroni) was used with P> 0.05. Results: The average weight of the extruded waste was 0.0134 ± 0.0157 for CT -1, 0.0075 ± 0.0062 for CT 00 and 0.0075 ± 0.0068 for CT +1, with no statistically significant differences between groups. Conclusion: There was no impact of the different CTs on the amount of extruded debris beyond the apex.

scholarly journals Predictive potential of the MELD and Child-Turcotte-Pugh II scores for SBP in patients with cirrhosis and ascites

2021 ◽  
Vol 13 (2) ◽  
pp. 1-9
Author(s):  
Fana Lichoska-Josifovikj ◽  
Kalina Grivcheva-Stardelova ◽  
Beti Todorovska ◽  
Magdalena Genadieva Dimitrovа ◽  
Lidija Petkovska ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Roc Analysis ◽  
Meld Score ◽  
Mean Values ◽  
Average Value ◽  
Percentage Difference ◽  
Class B ◽  
The Mean ◽  
The Difference ◽  
Difference Test

It is very important for patients with spontaneous bacterial peritonitis (SBP) to assess the length of survival and the risk of death, primarily because of the wide range of potential complications that can lead to multisystem organ failure and fatal outcome. The aim of this study was to determine the predictive potential of MELD and Child-Turcotte-Pugh II score for SBP in patients with cirrhosis and ascites. Material and methods: The study was designed as a prospective-analytical-observational and was conducted at the University Clinic for Gastroenterohepatology in Skopje for a period of one year. The study population included 70 hospitalized patients with established liver cirrhosis, regardless of etiology, divided into two groups, 35 patients with SBP and 35 non-SBP. Prognostic scores in patients with liver cirrhosis and ascites: MELD score, according to the formula: MELD = [(0.957 x Ln Creatinin) + (0.378 x Ln Bilirubin) + (1.12 x Ln INR) + (0.643) x 10]. The Child-Turcotte-Pugh II score includes 6 parameters: serum albumin and bilirubin, amount of ascites, degree of encephalopathy ( HE), prothrombin time (PT) and serum creatinine, and assessment of the degree of hepatic encephalopathy according to the West Haven criteria. Results: The average value of the MELD score in patients with SBP was 22.6 ± 8.27 and in non-SBP the average value was lower - 17.83±5.87. According to the Mann-Whitney U test, the difference between the mean values ​​was statistically significant for p <0.05 (z = 2.41; p = 0.015). A score of 30 to 39 was registered in 25.7% of patients with SBP, and only in 2.9% in non-SBP; the percentage difference was statistically significant for p <0.05 (Difference test, p = 0.0064 ). Patients with SBP had an average Child-Pugh score of 13.09 ± 2.48 or 100.0% C-class points. In patients with non-SBP, an average child-Pugh score of 9.63 ± 1.62 was recorded, or class B in 65.7% and class C in 34.3%. The percentage difference was statistically significant for p <0.05 (Difference test, p = 0.000000). According to the Mann-Whitney U test, the difference between the mean values ​​was statistically significant for p <0.05 (z = -5.44; p = 0.00001). ROC analysis indicated that the Child-Turcotte-Pugh II score contributed to the diagnosis of SBP - 90.7% (p = 0.000) (excellent predictor), closer to the ideal value of 1.0 and above the worst value of 0.5. ROC analysis indicated that the MELD score did not contribute to the diagnosis of SBP - 66.7% (p = 0.017) (weak predictor), closer to the worst value of 0.5. Conclusion:Our research confirmed that SBP occurs in patients with severe hepatic dysfunction calculated according to the CTP II score and MELD score. Mean value of the MELD score in patients with SBP was higher then in patients with non-SBP. On the other hand all patients with SBP had an average CTP II score, C-class points,  while the largest percentage of patients with non-SBP were class B-class points. MELD score is a weak predictor of SBP.  The best predictor for predicting SBP is the CTP II score (rank C).

The Effect of Jigsaw Methode and Motivation on Students’ of Learning Outcomes

2020 ◽  
Vol 3 (1) ◽  
pp. 20-30
Author(s):  
Yunita Yunita ◽  
Hidayat Hidayat ◽  
Harun Sitompul
Keyword(s):  
Cooperative Learning ◽  
Student Learning ◽  
Learning Outcomes ◽  
Student Learning Outcomes ◽  
Learning Motivation ◽  
Learning Approaches ◽  
High Learning ◽  
Average Value ◽  
The Difference ◽  
Low Motivation

This study aims to: (1) investigate the effect of Jigsaw cooperative learning on students learning outcomes; (2) find the difference in learning outcomes between high and low learning motivation and (3) find the interaction between learning approaches and learning motivation towards learning outcomes. The population of the study is students of grade IVa, IVb, IVc at SD Kasih Ibu Patumbak and the sample in this study is grade IVa with 35 students and grade IVb with 35 students. The results show that: (1) the average student learning outcomes of jigsaw cooperative learning is 28.40 while conventional is 24.14. Thus, students learning outcomes that get cooperative learning of jigsaw type are higher than conventional learning, (2) Students who have high motivation get an average value = 30.74, while low motivation is 22.72. Thus, it can be concluded that there are differences in student learning outcomes having high learning motivation and low learning motivation, and (3) students learning outcomes  taught by jigsaw cooperative learning are high learning motivation groups (32.94), and low learning motivation groups (24.58), while students taught with conventional learning are high learning motivation groups (28.40 ), and low motivation groups (20,95). Thus, there is no interaction between learning approaches and learning motivation towards learning outcomes.

Pengaruh Jumlah Pencucian Beras dengan Kadar Klorin

2017 ◽  
Vol 1 (1) ◽  
pp. 89
Author(s):  
Indah Purwaningsih ◽  
Supriyanto Supriyanto
Keyword(s):  
Mucous Membrane ◽  
Confidence Level ◽  
Iodometric Titration ◽  
Titration Method ◽  
Anova Test ◽  
Average Value ◽  
Significant Difference ◽  
The Difference

Abstract: Chlorine is a green halogen-shaped halogen gas at normal temperature and serves as bleach, stain remover and disinfectant. Chlorine is now widely used for bleaching rice so that less quality rice looks like quality rice. Chlorine is very toxic and causes mucous membrane irritation, highly reactive and very powerful oxidizer. The purpose of this research was to determine the difference of chlorine level in chlorinated rice washed once, twice and 3 times. The sample in this study amounted to 11 samples calculated by replication formula. Each sample was treated 3 times, ie 1 washed once, 2 washed twice and washed 3 times. The samples then examined by iodometric titration method. Based on the results of the study using ANOVA test, 11 samples obtained the average value of chlorine after washed once amount of 0.0176%, after washed twice amount of 0.0111%, and after washed 3 times amount of 0.0052% with the value significance p = 0.03 (p <0.05) at 95% confidence level which means there was a significant difference between chlorine levels in chlorinated rice washed once, twice and 3 times.Abstrak: Klorin merupakan unsur halogen berbentuk gas berwarna kuning kehijauan pada suhu normal danberfungsi sebagai pemutih, penghilang noda maupun desinfektan. Klorin sekarang banyak digunakan untuk bahan pemutih beras agar beras yang kurang berkualitas tampak seperti beras berkualitas. Klorin sangat toksik dan menyebabkan iritasi membran mukosa, sangat reaktif dan merupakan oksidator yang sangat kuat. Tujuan dari penelitian ini ialah untuk mengetahui perbedaan kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali. Sampel dalam penelitian ini berjumlah 11 sampel yang dihitung dengan rumus replikasi. Setiap sampel diberi perlakuan sebanyak 3 kali, yaitu 1 kali pencucian, 2 kali pencucian dan 3 kali pencucian. Sampel penelitian kemudian diperiksa dengan metode titrasi iodometri. Berdasarkan hasil penelitian menggunakan uji Anova secara komputerisasi terhadap 11 sampel diperoleh nilai rata-rata kadar klorin setelah 1 kali pencucian sebesar 0,0176 %, setelah 2 kali pencucian sebesar 0,0111 %, dan setelah 3 kali pencucian sebesar 0,0052 % dengan nilai signifkansi p = 0,03 (p<0,05) pada tingkat kepercayaan 95% yang artinya ada perbedaan yang bermakna antara kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali.

POTRET PERILAKU RELIGIUS MAHASISWA UIN SAYARIF HIDAYATULLAH JAKARTA

Al-Risalah ◽  
2018 ◽  
Vol 8 (2) ◽  
pp. 126-148
Author(s):  
Ay Maryani
Keyword(s):  
Religious Behavior ◽  
Male And Female ◽  
Mean Values ◽  
Significance Level ◽  
Average Value ◽  
Environment Variable ◽  
Independent Variable ◽  
The Mean ◽  
Gender Factor ◽  
Level 2

This study describes the religious behavior of UIN Syarif Hidayatullah Jakarta students. The variables used are internal and external environment as independent variable and religious behavior as dependent variable. The internal environment variable consists of gender factor, (male and female). The external environmental variables comprise the faculty environment, the present resident and the place to grow. The present resident consist of dormitories, boarding houses and parents' homes and the place to grow consist of urban and rural environment. Religious behavior variables consist of habluminallah behavior and habluminannas behavior. Habluminallah's behavior measured by (1) knowledge of faith and worship, (2) attitudes toward faith and worship, and (3) practice of faith and worship. The habluminannas variable measured by (1) Islamic behavior for them self, like honest, discipline and good work / studyethics, trust and concern on legality, (2) Islamic behavior with others, like generous, cooperation, caring, respect to the people's rights and tolerance and (3) Islamic behavior for the natural surroundings, like love of nature and nature conservation efforts. The methodology used was (1) Statistical descriptive, (2) MANOVA (Multivariate Analysis of Variance) and (3) Independent sample t-test. The results showed the religious behavior UIN Syarif Hidayatullah Jakarta students has a very good category. This is indicated by the mean values for the behavior of haluminallah and habluminannas of 158.85 and 178.76,  espectively. The average value of habluminallah behaviour in the range of values "145-180" with the category of "very good" and habluminannas behavior in the range value "165-205" with the category "very good. Habluminallah and habluminannas behavior are different for each faculty. This is indicated by the significance level of Pillai Trace, Wilk Lambda, Hotelling Trace, Roy's Largest Root of 0.00 (<0.05). Habluminallah and habluminannas behavior are the same for respondents who live in dormitories, boarding houses, and parents' homes. This is indicated by the value of F test and significance at Wilk's Lambda respectively for 2.055 and 0.085 (>0.05). Habluminallah and habluminannas behaviors are similar for urban and rural respondents. This is known from the sig level. (2-tailed) for habluminallah and habluminannas behavior of 0.317 and 0.245 (> 0.05), respectively. Habluminallah and habluminannas behaviors are similar for male and female. This is known from the sig level. (2-tailed) for habluminallah and habluminannas behavior of 0.950 and 0.307 (> 0.05),respectively. The results of this study are expected to be used to develop university policies that can enhance the Islamic values of UIN Syarif Hidayatullah Jakarta.

Results of studying the dimensional accuracy of the bases of complete removable prostheses made using 3D printing and traditional technologies

2020 ◽  
pp. 34-39
Author(s):  
Vokulova Yu.A. Vokulova ◽  
E.N. Zhulev
Keyword(s):  
3D Printing ◽  
Traditional Method ◽  
Laser Scanning ◽  
Laser Scanner ◽  
Digital Technologies ◽  
Dimensional Accuracy ◽  
3D Printer ◽  
Significance Level ◽  
Average Value ◽  
The Difference

This article presents the results of studying the dimensional accuracy of the bases of complete removable prostheses made using a 3D printer and the traditional method. Bases of complete removable prostheses were made using an intraoral laser scanner iTero Cadent (USA) and a 3D printer Asiga Max UV (Australia). To study the dimensional accuracy of the bases of complete removable prostheses, we used the DentalCAD 2.2 Valletta software. The Nonparametric Wilcoxon W-test was used for statistical analysis of the obtained data. We found that the average value of the difference with the standard for bases made using digital technologies is 0.08744±0.0484 mm. The average value of the difference with the standard for bases made by the traditional method is 0.5654±0.1611 mm. Based on these data, we concluded that the bases of complete removable prostheses made using modern digital technologies (intraoral laser scanning and 3D printer) have a higher dimensional accuracy compared to the bases of complete removable prostheses made using the traditional method with a significance level of p<0.05 (Wilcoxon's W-test=0, p=0.031). Keywords: digital technologies in dentistry, digital impressions, intraoral scanner, 3D printing, ExoCAD, complete removable dentures.

Seasonal trends in the stable isotopic composition of snow and meltwater runoff in a subarctic catchment at Okstindan, Norway

2004 ◽  
Vol 35 (2) ◽  
pp. 119-137 ◽  
Author(s):  
S.D. Gurney ◽  
D.S.L. Lawrence
Keyword(s):  
Isotopic Composition ◽  
Mean Value ◽  
Mean Values ◽  
Stable Isotopic Composition ◽  
Meltwater Runoff ◽  
Stable Isotopic ◽  
The Mean ◽  
The Difference ◽  
Δ18o And Δd

Seasonal variations in the stable isotopic composition of snow and meltwater were investigated in a sub-arctic, mountainous, but non-glacial, catchment at Okstindan in northern Norway based on analyses of δ18O and δD. Samples were collected during four field periods (August 1998; April 1999; June 1999 and August 1999) at three sites lying on an altitudinal transect (740–970 m a.s.l.). Snowpack data display an increase in the mean values of δ18O (increasing from a mean value of −13.51 to −11.49‰ between April and August), as well as a decrease in variability through the melt period. Comparison with a regional meteoric water line indicates that the slope of the δ18O–δD line for the snowpacks decreases over the same period, dropping from 7.49 to approximately 6.2.This change points to the role of evaporation in snowpack ablation and is confirmed by the vertical profile of deuterium excess. Snowpack seepage data, although limited, also suggest reduced values of δD, as might be associated with local evaporation during meltwater generation. In general, meltwaters were depleted in δ18O relative to the source snowpack at the peak of the melt (June), but later in the year (August) the difference between the two was not statistically significant. The diurnal pattern of isotopic composition indicates that the most depleted meltwaters coincide with the peak in temperature and, hence, meltwater production.

scholarly journals One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Joseph R. Owen ◽  
Sadie L. Hennig ◽  
Bret R. McNabb ◽  
Tamer A. Mansour ◽  
Justin M. Smith ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Bos Taurus ◽  
Single Copy ◽  
Blastocyst Stage ◽  
Target Site ◽  
End Joining ◽  
Bull Calf ◽  
Dividing Cells ◽  
Green Fluorescent ◽  
Donor Template

Abstract Background The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has been shown to increase knock-in efficiency relative to HR, non-homologous end joining (NHEJ), and microhomology-mediated end joining (MMEJ) strategies in non-dividing cells. Results By introducing gRNA/Cas9 ribonucleoprotein complex and a HMEJ-based donor template with 1 kb homology arms flanked by the H11 safe harbor locus gRNA target site, knock-in rates of 40% of a 5.1 kb bovine sex-determining region Y (SRY)-green fluorescent protein (GFP) template were achieved in Bos taurus zygotes. Embryos that developed to the blastocyst stage were screened for GFP, and nine were transferred to recipient cows resulting in a live phenotypically normal bull calf. Genomic analyses revealed no wildtype sequence at the H11 target site, but rather a 26 bp insertion allele, and a complex 38 kb knock-in allele with seven copies of the SRY-GFP template and a single copy of the donor plasmid backbone. An additional minor 18 kb allele was detected that looks to be a derivative of the 38 kb allele resulting from the deletion of an inverted repeat of four copies of the SRY-GFP template. Conclusion The allelic heterogeneity in this biallelic knock-in calf appears to have resulted from a combination of homology directed repair, homology independent targeted insertion by blunt-end ligation, NHEJ, and rearrangement following editing of the gRNA target site in the donor template. This study illustrates the potential to produce targeted gene knock-in animals by direct cytoplasmic injection of bovine embryos with gRNA/Cas9, although further optimization is required to ensure a precise single-copy gene integration event.

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