Polyamide/Chitosan/Tetraethyl Orthosilicate Electrospun Nanofibers for a Novel and Promising Drug Carrier

Chitosan (CS), the only alkaline polysaccharose available in nature, has always been a promising candidate for drug delivery owing to its excellent biodegradability and biocompatibility. However, inherent solubility and polycationic properties of CS largely hinder electrospinning, which is an efficient method of fabricating nanofibers for drug carriers. To solve this problem and extend the applications of CS, polyamide/chitosan/tetraethyl orthosilicate (PA/CS/TEOS) composite nanofibers were successfully prepared as drug carriers in this study via electrospinning. The PA/CS/TEOS ratios significantly influenced the nanofiber morphology. As the content of each was increased, the beads among the membranes increased initially and then decreased, determined by scanning electron microscopy (SEM). The morphology of the optimum membranes with the ratio of 1:0.13:0.67 was smoother with less beads and uniform fiber diameter. Finally, the membranes with optimum ratios were used as carriers of ofloxacin in the study of drug release performance to identify their future feasibility, which revealed an initial fast release followed by a relatively stable release.

Analyzing Static Analysis Metric Trends towards Early Identification of Non-Maintainable Software Components

Nowadays, agile software development is considered a mainstream approach for software with fast release cycles and frequent changes in requirements. Most of the time, high velocity in software development implies poor software quality, especially when it comes to maintainability. In this work, we argue that ensuring the maintainability of a software component is not the result of a one-time only (or few-times only) set of fixes that eliminate technical debt, but the result of a continuous process across the software’s life cycle. We propose a maintainability evaluation methodology, where data residing in code hosting platforms are being used in order to identify non-maintainable software classes. Upon detecting classes that have been dropped from their project, we examine the progressing behavior of their static analysis metrics and evaluate maintainability upon the four primary source code properties: complexity, cohesion, inheritance and coupling. The evaluation of our methodology upon various axes, both qualitative and quantitative, indicates that our approach can provide actionable and interpretable maintainability evaluation at class level and identify non-maintainable components around 50% ahead of the software life cycle. Based on these results, we argue that the progressing behavior of static analysis metrics at a class level can provide valuable information about the maintainability degree of the component in time.

Smart textile device for shooter’s fingers movement monitoring

BACKGROUND: The manner in which shooters pull the trigger may significantly affect the shooter’s results. Shooting coaches are often not able to detect incorrect pull because of gun movement during the shot and recoil. OBJECTIVE: Development of the smart-textile based trigger pull monitoring system and demonstration of its ability to distinguish correct and wrong triggering techniques. METHODS: Two separated knitted resistive pressure sensors were integrated over III and II phalanges in the index finger fingerstall; single sensor was integrated over both III and II phalanges of the middle finger fingerstall. Resistance of the sensors was measured in a course of shots, performed by expert shooter, which simulated typical novice’s trigger pull errors. RESULTS: Sensors’ resistance recordings were made for following erroneous trigger pull motions: pulling of the trigger with index finger’s II phalanx instead of III; fast and jerky trigger pull (trigger tear-off); too fast release of the trigger after shot; and excessive grip force, applied by middle finger. For each type of erroneous movement, recordings waveforms included distinguishable features that characterised a particular type of error. CONCLUSIONS: The developed trigger pull monitoring system provides signals that could be used for recognition of the incorrect trigger pull motions during gun shots.

Chitosan Oleate Coated PLGA Nanoparticles as siRNA Drug Delivery System

Oligonucleotide therapeutics such as miRNAs and siRNAs represent a class of molecules developed to modulate gene expression by interfering with ribonucleic acids (RNAs) and protein synthesis. These molecules are characterized by strong instability and easy degradation due to nuclease enzymes. To avoid these drawbacks and ensure efficient delivery to target cells, viral and non-viral vectors are the two main approaches currently employed. Viral vectors are one of the major vehicles in gene therapy; however, the potent immunogenicity and the insertional mutagenesis is a potential issue for the patient. Non-viral vectors, such as polymeric nanocarriers, provide a safer and more efficient delivery of RNA-interfering molecules. The aim of this work is to employ PLGA core nanoparticles shell-coated with chitosan oleate as siRNA carriers. An siRNA targeted on HIV-1, directed against the viral Tat/Rev transcripts was employed as a model. The ionic interaction between the oligonucleotide’s moieties, negatively charged, and the positive surface charges of the chitosan shell was exploited to associate siRNA and nanoparticles. Non-covalent bonds can protect siRNA from nuclease degradation and guarantee a good cell internalization and a fast release of the siRNA into the cytosolic portion, allowing its easy activation.

Porphyrin Based 2D-MOF Structures as Dual-Kinetic Sorafenib Nanocarriers for Hepatoma Treatment

The existing clinical protocols of hepatoma treatment require improvement of drug efficacy that can be achieved by harnessing nanomedicine. Porphyrin-based, paddle-wheel framework (PPF) structures were obtained and tested as dual-kinetic Sorafenib (SOR) nanocarriers against hepatoma. We experimentally proved that sloughing of PPF structures combined with gradual dissolving are effective mechanisms for releasing the drug from the nanocarrier. By controlling the PPF degradation and size of adsorbed SOR deposits, we were able to augment SOR anticancer effects, both in vitro and in vivo, due to the dual kinetic behavior of SOR@PPF. Obtained drug delivery systems with slow and fast release of SOR influenced effectively, although in a different way, the cancer cells proliferation (reflected with EC50 and ERK 1/2 phosphorylation level). The in vivo studies proved that fast-released SOR@PPF reduces the tumor size considerably, while the slow-released SOR@PPF much better prevents from lymph nodes involvement and distant metastases.

Effective DevSecOps Implementation: A Systematic Literature Review

Adopting DevOps means increased collaboration between development and operations teams and faster release cycles through a shift to automation. Using Dev Ops brings with it several advantages in the development of software. Security, however, is often neglected in DevOps due to the fast release cycle. Therefore Dev Sec Ops has emerged as an extension to DevOps that attempts to integrate security with Dev Ops practices, which is not without its challenges. DevOps, and by extension Dev Sec Ops, represents a significant change in the culture, tooling, and processes used in software development. Therefore, when implementing DevSecOps, teams and their organizations need to be aware of the challenges it brings and how to address those challenges for a DevSecOps implementation to be effective. Literature on DevSecOps exists that outlines practices and principles to do this. This paper uses a grounded theory approach to do a systematic literature review of academic literature to find the factors that contribute to an effective DevSecOps implementation. It attempts to reconcile the challenges of DevSecOps with ways of mitigating them and the advantages that a DevSecOps implementation can bring. The paper thus outlines methods of effectively implementing DevSecOps as described in academic literature.

Influence of Flue Gas Desulfurization Gypsum on Phosphorus Loss from a Horticultural Growth Medium

In response to agriculture’s contribution to surface water quality, considerable effort is being made to develop best management practices to reduce nutrient loss. To evaluate the efficacy of gypsum as a horticultural media amendment for controlling phosphorus (P) leaching, flue gas desulfurization (FGD) gypsum was added to a standard horticultural growth medium at 0, 2.5, 5, 10 or 15% (v/v). FGD gypsum was either mixed with the growing medium or placed at the bottom of the containers. A fast-release or a control-release fertilizer was top-dressed to containers. The greatest P leaching occurred with the fertilizer-only treatments (no gypsum). Dissolved reactive P (DRP) losses were highest on the initial day of measurement for the fast-release fertilizer and then decreased rapidly. There was a delayed release of DRP from the controlled-release fertilizer. Increasing rates of FGD gypsum addition resulted in decreasing DRP leaching concentration loss and load. The FGD gypsum decreased leachate DRP concentration loss by a maximum of 75%, with an average decrease of 46%. Mixing the FGD gypsum with the medium (an easier/less expensive means of incorporation) was most effective with the fast-release fertilizer. These preliminary results indicate that less gypsum may be needed to reduce P loss from fast-released fertilizer as opposed to control-release fertilizer. FGD gypsum remained effective in reducing DRP loss throughout the experiment.