Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.

Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya

The condition of eating places such as street vendors will determine the level of food hygiene, including the water used to wash food utensils. This study aimed to determine the number of microbes and identify Escherichia coli and Salmonella sp. on the water that was used to wash tableware by street vendors around UIN Sunan Ampel Surabaya. Water samples before and after being used to wash tableware were obtained from 4 street vendors. The number of microbes in the sample was tested by the Total Plate Count (TPC) and Most Probable Number (MPN) methods, Escherichia coli was identified using Eosin Methylene Blue Agar (EMB) media, and Salmonella sp. was identified using Salmonella-Shigella agar (SSA) and Triple Sugar Iron agar (TSIA) media. Based on the results of TPC test, the water sample before being used to wash tableware had the lowest and highest values that were 3.0 x 102 Cfu/ml and 9.8 x 105 Cfu/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 4.0 x 102 Cfu/ml and 3.5 x 105 Cfu/ml, respectively. In the MPN test, the water sample before being used to wash tableware had the lowest and highest values that were 15/100ml and 26 100/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 72/100 ml and 271/ 100 ml, respectively. Escherichia coli was detected in all samples 1&3 while in sample 2 Escherichia coli was detected in the water before it was detected the genus Enterobacter and in samples 4 the genus Enterobacter was detected and all samples water used to wash tableware of street vendors was positive for Salmonella.

Identifikasi Kualitas Air Sumur, Air dalam Kemasan, dan Air Sungai dengan Metode Most Probable Number

Air memiliki peranan yang sangat besar salah satunya untuk kebutuhan biologisnya, yaitu bertahan hidup. Masalah utama yang dihadapi berkaitan dengan sumber daya air adalah kualitas air. Penentuan kualitas air secara mikrobiologi dilakukan dengan Most Probable Number (MPN) Test. Penelitian ini menggunakan 3 sampel yaitu air sumur, air dalam kemasan, dan air sungai. Air sumur mengandung Coliform dan Escherichia coli dengan jumlah yang sama yaitu 14 MPN/100 mL. Pada uji dugaan, air dalam kemasan menunjukkan hasil positif, namun saat diuji penetapan diperoleh hasil negatif. Sedangkan pada air sungai mengandung bakteri coliform lebih dari 1100 MPN/ 100 mL dan Escherichia coli sebanyak 11 MPN/ 100 mL. Sampel air minum dalam kemasan layak dikonsumsi sedangkan untuk sampel air sumur dan air sungai tidak layak dikonsumsi karena mengandung Coliform dan Escherichia coli.

Microbial Content Test on Sliced Papaya in Area of Universitas Sumatera Utara

Papaya is a fruit that is often consumed in Indonesia and has increased consumption from 2015-2016 based on SUSENAS 2016. Papaya has high water content (85-90%) and the pH is near neutral. This makes papaya vulnerable and suitable medium for the growth of pathogenic microbes. The presence of pathogenic microbes in papaya can cause health problems for those who consumed them. Objectives this study was to observe the microbial content of sliced papaya in the Universitas Sumatera Utara. Method this research was carried out by conducting laboratory tests and the results obtained in the form of a description of microbial content in papaya cut samples. The content of coliform microbes in sliced papaya was tested using the Most Probable Number (MPN) method and biochemical identification. Papaya samples were selected using total sampling method from the University of Sumatera Utara. Results A total of 14 sliced papaya samples were analysed in this study. By using the MPN test it was found that all papaya samples had a microbial threshold exceeding those determined by SNI 7388: 2009 which is below <20 / g. A total of 9 papaya samples (64.28%) contained Klebsiella sp. bacteria, 5 samples (35.72%) contained E.coli bacteria. Conclusions Bacteria found in this study were Escherichia coli and Klebsiella sp. The presence of microbial content in the sliced papaya sample that exceeds the threshold must be a concern of various parties in the surveillance of snacks being sold.

Challenging the WHO Prescribed Sanitary Inspections for Contamination of Tubewells with Handpump

WHO prescribed sanitary inspections constitute useful monitoring tools, besides being effective and economic in identifying drinking sources of water at risk in terms of contamination. In the present case, the contamination risks in potable waters from Tubewells fitted with handpump were evaluated across 9 districts in India. Field inspections were conducted in WHO prescribed format on Three Hundred Twenty Four sources of potable water used by the community for withdrawing drinking water to evaluate occurrence of contamination risk. The theoretical risk thus assessed was then compared with actual test results of biological testing in the laboratory for assessment of Coliform bacteria using a standard MPN test. The results of the study indicate that WHO format based sanitary survey results are more stringent than reality, and hence overestimate the risk. The results also indicate that the risk categories are mostly distributed with a majority of risk focused in the middle in the High and Medium risk categories.

NILAI MPN (Most Probable Number) DAN DETEKSI BAKTERI Esherichia PADA MINUMAN ES JERUK PERAS di KOTA PONTIANAK

Iced squeezed orange drinks is one one of processed drinks that made from orange. Good drinking water should not contain pathogenic bacteria or organisms that may harm human health. Coliform is one ofbacteria that may cause disease in humans and being used as indicator bacteria of pathogenic bacteria. This research was aimed to know the MPN value number of coliform bacteria and to identify the bacterial presence of genus Escherichia in iced squeezed orange drinks sold in Pontianak City. The sampling used random sampling method. MPN method was used in order to know the number of coliform in iced squeezed orange drinks in Pontianak City. MPN test result obtained the lowest index value of MPN/mL was 0,14/mL in sample in subdistrict Pontianak City and the highest index value in four subdistricts in Pontianak City was MPN/mL >24,00/mL. It was showed that five samples exceed normal limits in accordance with SNI standards SNI 01-3839-1995 based on Permenkes No 492/MENKES/per/IV/2010. Identification resultshowed the bacterial genus of coliform obtained was member of genus Escherichia.

IDENTIFICATION OF HAZARDOUS CHEMICAL AND MICROBES CONTAMINATION IN FOODS OF STREET VENDORS AROUND IAIN SYEKH NURJATI CIREBON

This study aimed at identifying chemical and microorganism contamination in foods of street vendors around Institut Agama Islam (IAIN) Syekh Nurjati Cirebon. The samples are meatball, cilok, grilled fish cake, nuggets, rolade, sauce, crackers, and iced water drinks. The sample tests are formalin test, borax test, Rodhamine B test, Methanyl Yellow test, and Coliform & Escherichia coli MPN test. The results showed that some food samples like meatball, cilok, grilled fish cake, nuggets,and rolade were positive of formalin & borax contaminated. The iced water drinks are contaminated by Coliform & E. coli. The other samples like sauce and crackers were negative of Rhodamine B & Methanyl Yellow contaminated. In conclusion, according to this study, the foods of street vendors at IAIN Cirebon are not yet safe to be consumed.