scholarly journals Microbial Content Test on Sliced Papaya in Area of Universitas Sumatera Utara

2020 ◽  
Vol 3 (2) ◽  
pp. 86-92
Author(s):  
Nenni Dwi Aprianti Lubis ◽  
Sri Amelia ◽  
Ridwan Balatif
Keyword(s):  
Laboratory Tests ◽  
Sampling Method ◽  
High Water ◽  
Most Probable Number ◽  
High Water Content ◽  
Probable Number ◽  
Biochemical Identification ◽  
Pathogenic Microbes ◽  
The University ◽  
Mpn Test

Papaya is a fruit that is often consumed in Indonesia and has increased consumption from 2015-2016 based on SUSENAS 2016. Papaya has high water content (85-90%) and the pH is near neutral. This makes papaya vulnerable and suitable medium for the growth of pathogenic microbes. The presence of pathogenic microbes in papaya can cause health problems for those who consumed them. Objectives this study was to observe the microbial content of sliced papaya in the Universitas Sumatera Utara. Method this research was carried out by conducting laboratory tests and the results obtained in the form of a description of microbial content in papaya cut samples. The content of coliform microbes in sliced papaya was tested using the Most Probable Number (MPN) method and biochemical identification. Papaya samples were selected using total sampling method from the University of Sumatera Utara. Results A total of 14 sliced papaya samples were analysed in this study. By using the MPN test it was found that all papaya samples had a microbial threshold exceeding those determined by SNI 7388: 2009 which is below <20 / g. A total of 9 papaya samples (64.28%) contained Klebsiella sp. bacteria, 5 samples (35.72%) contained E.coli bacteria. Conclusions Bacteria found in this study were Escherichia coli and Klebsiella sp. The presence of microbial content in the sliced papaya sample that exceeds the threshold must be a concern of various parties in the surveillance of snacks being sold.

scholarly journals NILAI MPN (Most Probable Number) DAN DETEKSI BAKTERI Esherichia PADA MINUMAN ES JERUK PERAS di KOTA PONTIANAK

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Ratna Ratna ◽  
Rahmawati Rahmawati ◽  
Mukarlina Mukarlina
Keyword(s):  
Random Sampling ◽  
Pathogenic Bacteria ◽  
Sampling Method ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Normal Limits ◽  
Index Value ◽  
Mpn Test ◽  
Test Result

Iced squeezed orange drinks is one one of processed drinks that made from orange. Good drinking water should not contain pathogenic bacteria or organisms that may harm human health. Coliform is one ofbacteria that may cause disease in humans and being used as indicator bacteria of pathogenic bacteria. This research was aimed to know the MPN value number of coliform bacteria and to identify the bacterial presence of genus Escherichia in iced squeezed orange drinks sold in Pontianak City. The sampling used random sampling method. MPN method was used in order to know the number of coliform in iced squeezed orange drinks in Pontianak City. MPN test result obtained the lowest index value of MPN/mL was 0,14/mL in sample in subdistrict Pontianak City and the highest index value in four subdistricts in Pontianak City was MPN/mL >24,00/mL. It was showed that five samples exceed normal limits in accordance with SNI standards SNI 01-3839-1995 based on Permenkes No 492/MENKES/per/IV/2010. Identification resultshowed the bacterial genus of coliform obtained was member of genus Escherichia.

Colony-Forming Unit Enumeration by a Plate-MPN Method

1983 ◽  
Vol 46 (10) ◽  
pp. 836-841 ◽  
Author(s):  
S-T. TAN ◽  
R. B. MAXCY ◽  
W. W. STROUP
Keyword(s):  
Standard Technique ◽  
Most Probable Number ◽  
Plate Method ◽  
Probable Number ◽  
Microbial Counts ◽  
Colony Forming Unit ◽  
Pure Cultures ◽  
Petri Plate ◽  
Mpn Test ◽  
Surface Plate

Concepts of the standard surface plate method and the most probable number method (MPN) were combined to provide a new enumeration technique (plate-MPN). Three discrete 0.01-ml samples of an appropriate decimal dilution were inoculated onto each quadrant of a pre-dried petri plate. The discrete spots from the inoculum were then observed for growth after incubation. Results were interpreted analogous to a 3-tube MPN test using presently available tables. Application of the test to pure cultures and mixed flora provided no evidence to indicate the plate-MPN technique to be any less accurate than the standard technique for microbial counts. The plate-MPN technique was less precise than the standard technique. However, the plate-MPN technique has many advantages over traditional methods.

scholarly journals Kualitas Air Sumur Gali Kelurahan Lubuk Buaya Kecamatan Koto Tangah Kota Padang Berdasarkan Indeks Most Probable Number (MPN)

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Randa Novalino ◽  
Netti Suharti ◽  
Arni Amir
Keyword(s):  
Water Quality ◽  
Health Department ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Well Water ◽  
Health Minister ◽  
Probable Number ◽  
Sources Of Pollution ◽  
Dug Well ◽  
Mpn Test

AbstrakDiare merupakan salah satu penyakit yang ditularkan melalui air terkontaminasi oleh agen penyebab seperti bakteri Coliform.  Menurut data Dinas Kesehatan Kota (DKK) Padang pada tahun 2011, kejadian diare di Kelurahan Lubuk Buaya Kecamatan Koto Tangah, Kota Padang merupakan kasus tertinggi di Kota Padang. Tujuan penelitian ini adalah menentukan kualitas air sumur gali di Kelurahan Lubuk Buaya berdasarkan Indeks Most Probable Number (MPN) menurut Peraturan Menteri Kesehatan Republik Indonesia (Permenkes RI) No. 416 tahun 1990. Sampel penelitian ini adalah  air sumur gali yang digunakan di beberapa Rukun Tetangga (RT), yang diambil secara acak dari beberapa Rukun Warga (RW) yang telah dipilih sebelumnya, sehingga didapatkan 15 sampel. Penelitian ini dilaksanakan dalam 2 tahap yaitu pengambilan sampel air sumur gali sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologi dengan metode Most Probable Number (MPN) Test. Tes ini terdiri dari tes presumtif dan tes konfirmatif yang disesuaikan dengan Permenkes RI. Hasil penelitian ialah 73,33% dari jumlah sumur yang diperiksa tidak memenuhi standar Permenkes R.I. karena mengandung Coliform > 50 pada setiap 100 ml air. Hanya 26,6% sumur yang memenuhi standar yang telah ditetapkan. Beberapa faktor yang dapat mempengaruhi yaitu lokasi sumber pencemaran, dinding parapet, drainase, tutup sumur dan sarana pengambilan air.Kata kunci: kualitas air sumur gali, MPN, coliform AbstractDiarrhea is one of the diseases that  transmitted through contaminated water by causative agent, one of which is coliform bacteria. According to data from City Health Department Padang in 2011, the incidence of diarrhea in Kelurahan Lubuk Buaya Kecamatan Koto Tangah – Padang is the highest case in the city of Padang. The objective of this study was to determine the water quality of wells dug in Kelurahan Lubuk Buaya by Most Probable Number Index (MPN) according regulation of Indonesian health minister. The 15 samples was water of dug well  in some of the RT households from several neighborhoods that was selected. The research was conducted in two stages, dug well water samples as well as observation of the factors that affect water quality and microbiological examination of the Most Probable Number method (MPN) Test. This test consists of presumptive tests and confirmative tests that were tailored to regulation of Indonesian health minister.  The result were 73.33% of the wells tested did not meet the standards of Indonesian health minister regulation, because it contains >50 coliform in every 100 ml of water. Only 26.6% of the wells were inspected to meet the standards set. Several factors can affect the location of sources of pollution, parapet walls, drainage or sewer water, cover the wells, and water collection facilities.Keywords: dug well water quality, MPN, coliform

Identifikasi Kualitas Air Sumur, Air dalam Kemasan, dan Air Sungai dengan Metode Most Probable Number

2021 ◽  
Vol 10 (1) ◽  
pp. 40-45
Author(s):  
Ni Putu Trisna Ayundita
Keyword(s):  
Escherichia Coli ◽  
Most Probable Number ◽  
Probable Number ◽  
Mpn Test

Air memiliki peranan yang sangat besar salah satunya untuk kebutuhan biologisnya, yaitu bertahan hidup. Masalah utama yang dihadapi berkaitan dengan sumber daya air adalah kualitas air. Penentuan kualitas air secara mikrobiologi dilakukan dengan Most Probable Number (MPN) Test. Penelitian ini menggunakan 3 sampel yaitu air sumur, air dalam kemasan, dan air sungai. Air sumur mengandung Coliform dan Escherichia coli dengan jumlah yang sama yaitu 14 MPN/100 mL. Pada uji dugaan, air dalam kemasan menunjukkan hasil positif, namun saat diuji penetapan diperoleh hasil negatif. Sedangkan pada air sungai mengandung bakteri coliform lebih dari 1100 MPN/ 100 mL dan Escherichia coli sebanyak 11 MPN/ 100 mL.  Sampel air minum dalam kemasan layak dikonsumsi sedangkan untuk sampel air sumur dan air sungai tidak layak dikonsumsi karena mengandung Coliform dan Escherichia coli.

scholarly journals Evaluation of an MPN test for the rapid enumeration of Pseudomonas aeruginosa in hospital waters

2014 ◽  
Vol 13 (2) ◽  
pp. 427-436 ◽  
Author(s):  
David P. Sartory ◽  
Danièle Pauly ◽  
Nathalie Garrec ◽  
Lucia Bonadonna ◽  
Maurizio Semproni ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Filtration ◽  
Most Probable Number ◽  
Insufficient Data ◽  
Culture Methods ◽  
Probable Number ◽  
Rapid Enumeration ◽  
Relative Differences ◽  
Mpn Test ◽  
Atypical Strains

In this study, the performance of a new most probable number (MPN) test (Pseudalert®/Quanti-Tray®) for the enumeration of Pseudomonas aeruginosa from hospital waters was compared with both international and national membrane filtration-based culture methods for P. aeruginosa: ISO 16266:2006 and UK The Microbiology of Drinking Water – Part 8 (MoDW Part 8), which both use Pseudomonas CN agar. The comparison based on the calculation of mean relative differences between the two methods was conducted according to ISO 17994:2014. Using both routine hospital water samples (80 from six laboratories) and artificially contaminated samples (192 from five laboratories), paired counts from each sample and the enumeration method were analysed. For routine samples, there were insufficient data for a conclusive assessment, but the data do indicate at least equivalent performance of Pseudalert®/Quanti-Tray®. For the artificially contaminated samples, the data revealed higher counts of P. aeruginosa being recorded by Pseudalert®/Quanti-Tray®. The Pseudalert®/Quanti-Tray® method does not require confirmation testing for atypical strains of P. aeruginosa, saving up to 6 days of additional analysis, and has the added advantage of providing confirmed counts within 24–28 hours incubation compared to 40–48 hours or longer for the ISO 16266 and MoDW Part 8 methods.

scholarly journals Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi

2021 ◽  
Vol 4 (2) ◽  
pp. 207
Author(s):  
Achmad Arby Wijaya ◽  
Iwan Sahrial Hamid ◽  
Maya Nurwartanti Yunita ◽  
Wiwiek Tyasningsih ◽  
Ratih Novita Praja
Keyword(s):  
Escherichia Coli ◽  
Total Sample ◽  
Cow Milk ◽  
Most Probable Number ◽  
Methyl Red ◽  
Probable Number ◽  
E Coli ◽  
Fresh Milk ◽  
Peptone Water ◽  
Mpn Test

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.

scholarly journals Uji Bakteriologis pada Minuman Air Tebu yang Dijual di Pinggiran Jalan Khatib Sulaiman Kota Padang

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Dewi Oktavia Djasmi ◽  
Roslaili Rasyid ◽  
Eliza Anas
Keyword(s):  
Sugar Cane ◽  
Pathogenic Bacteria ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Index Method ◽  
Probable Number ◽  
E Coli ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Mpn Test

Abstrak Higinitas penjual dan sanitasi lingkungan yang kurang bersih memungkinkan minuman tebu terkontaminasi oleh bakteri patogen. Lokasi penjualan minuman tebu pinggiran jalan yang terbanyak terletak di jalan Khatib Sulaiman kota Padang. Tujuan penelitian ini adalah menentukan kualitas minuman tebu apakah sesuai dengan persyaratanmikrobiologi.  Penelitian ini bersifat deskriptif dengan menggunakan metode indeks Most Probable Number (MPN) di bagian Mikrobiologi Fakultas Kedokteran Universitas Andalas Padang. Sampel pada penelitian ini adalah seluruh penjual minuman tebu yang ada, yaitu sepuluh minuman tebu yang berasal dari lima pedagang. Sepuluh sampel terdiridari lima minuman tebu yang dicampur es dan lima minuman tebu yang tidak dicampur es. Pemeriksaan MPN yang telah dilakukan terdiri dari dua tes, yaitu: tes presumtif dan tes konfirmatif. Hasilnya adalah seluruh sampel positif mengandung bakteri Coliform dan E. coli. Kesimpulan dari penelitian ini adalah air tebu yang dijual di pinggiran jalan Khatib Sulaiman Padang belum memenuhi standar kelayakan konsumsi secara bakteriologis dan terdapat perbedaan indeks MPN antara minuman tebu yang dicampur es dengan yang tidak dicampur es.Kata kunci: tes MPN, air tebu, hygiene Abstract Pathogenic bacteria can contaminate the sugar cane juice by the less hygiene and environmental sanitation of the sellers. There are a lot of people selling sugar cane juice in Padang, mostly in Khatib Sulaiman. The objective of this study was to determine the quality of the sugar cane juice accordance to microbiological requirements. This descriptive study was using the most probable number (MPN) index method and conducted in Microbiology Laboratory of Medical Faculty, Andalas University Padang.The sample in this study is all of sellers, which is ten cane juices fromfive sellers. Ten samples consists of five sugar cane water with ice and sugar cane water without ice MPN test was done by two tests, the presumptive test and the confirmative test. The result showed that all samples contained coliform bacteria and E.coli. The conclusion of this study is sugarca ne juice which is sold on roadside of Khatib Sulaiman is not worthy to microbiological standards for consumption and there is a difference between the MPN index sugar cane water with ice and sugar cane without ice.Keywords: MPN test, cane juice, hygiene

Evaluation of Most-Probable-Number–PCR Method with Internal Amplification Control for the Counting of Total and Pathogenic Vibrio parahaemolyticus in Frozen Shrimps

2012 ◽  
Vol 75 (1) ◽  
pp. 150-153 ◽  
Author(s):  
S. COPIN ◽  
A. ROBERT-PILLOT ◽  
P. MALLE ◽  
M. L. QUILICI ◽  
M. GAY
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Direct Detection ◽  
Selective Medium ◽  
Internal Amplification Control ◽  
Most Probable Number ◽  
Probable Number ◽  
Pathogenic Vibrio ◽  
Biochemical Identification ◽  
Pcr Method ◽  
Frozen Shrimp

The most-probable-number (MPN) method is often time-consuming for the isolation, detection, and quantification of Vibrio parahaemolyticus from natural sources. MPN counting of V. parahaemolyticus bacteria usually involves the isolation of typical V. parahaemolyticus colonies on selective medium, with subsequent confirmation by biochemical identification. In this study, we evaluated the use of a PCR on MPN enrichment cultures (MPN-PCR) for the direct detection of total and pathogenic V. parahaemolyticus cells in frozen shrimp. This reaction targeted the R72H, tdh, and trh sequences. An internal amplification control was added to the samples before R72H amplification. There was an excellent correlation between the results of the two methods for artificially inoculated and natural shrimp samples. Of 36 natural samples, 28 tested positive for the presence of V. parahaemolyticus, with an MPN value of 2 × 10−1 to 9.2 × 101 per g. No pathogenic V. parahaemolyticus cells were detected. The test had a detection limit of one V. parahaemolyticus organism per g and was completed within two working days. These results support the use of the combination of PCR with MPN for the detection of total or potentially pathogenic V. parahaemolyticus cells in frozen shrimp.

scholarly journals Experimental analysis of the effect of electrokinetic treatment of soils

2019 ◽  
Vol 92 ◽  
pp. 11020
Author(s):  
Sara Gargano ◽  
Stefania Lirer ◽  
Barbara Liguori ◽  
Alessandro Flora
Keyword(s):  
Mechanical Properties ◽  
Sea Water ◽  
Tap Water ◽  
Chemical Properties ◽  
High Water ◽  
High Water Content ◽  
Electrokinetic Treatment ◽  
Macro Scale ◽  
Long Time ◽  
The University

In Europe, large amounts of materials are dredged annually to maintain channels and harbour activities. Consolidation processes in dredged sediments take very long-time due to the high-water content, much more than those considered in the typical problems of geotechnical engineering. The electrokinetic treatment (EK) can be an effective technique to accelerate the dewatering of these sediments and, at the same time, to improve their mechanical properties. An experimental research is under course at the University of Napoli Federico II with the aim to analyse the effects induced by EK treatments on the physic-chemical and mechanical properties of two different soils. Some laboratory tests have been carried out in a special apparatus (special oedometer) adopting two different pore fluids (tap water and sea water). The laboratory results show that the effect of EK treatment, both in terms of induced water flow and improvement of soil mechanical properties, is affected by the chemical properties of the pore fluid and by the physical and mineralogical characteristics of the particles. The results obtained at the macro-scale (specimen) have been analysed at the micro-scale by means of X-ray diffraction analysis and Scanning Electron Microscopy, comparing the microstructures of treated and untreated soils specimens.

scholarly journals ISOLASI Escherichia coli PADA DAGING SAPI SEGAR YANG DIPEROLEH DARI BEBERAPA PASAR TRADISIONAL DI PEKANBARU

2016 ◽  
Vol 7 (01) ◽  
pp. 121-126
Author(s):  
Elsie ◽  
Israwati Harahap
Keyword(s):  
Escherichia Coli ◽  
Most Probable Number ◽  
Methyl Red ◽  
Human Intestine ◽  
Isolation And Identification ◽  
Probable Number ◽  
E Coli ◽  
Traditional Markets ◽  
The Media ◽  
Mpn Test

Escherichia coli is a bacteria that inhabitant in the cattle or human intestine. Raw beef sold in the traditional markets is easily to contaminated by E. coli. It’s because the markets is one of the vulnerable and the risk is high enough against contamination. The goal of this research is to detect E. coli contamination in the fresh raw beef at several markets in Pekanbaru. This research used 100 gr fresh raw beef purchased from the six traditional markets in Pekanbaru. The study begins with a Most probable Number (MPN) test, than isolation and identification of the media ECB and L-EMB, initially with IMViC test (Indol, Methyl Red, Voges Praskauer and Citrate). Results of MPN test showed that all raw beef were contaminated E.coli with MPN varying value.

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