scholarly journals NILAI MPN (Most Probable Number) DAN DETEKSI BAKTERI Esherichia PADA MINUMAN ES JERUK PERAS di KOTA PONTIANAK

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Ratna Ratna ◽  
Rahmawati Rahmawati ◽  
Mukarlina Mukarlina
Keyword(s):  
Random Sampling ◽  
Pathogenic Bacteria ◽  
Sampling Method ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Normal Limits ◽  
Index Value ◽  
Mpn Test ◽  
Test Result

Iced squeezed orange drinks is one one of processed drinks that made from orange. Good drinking water should not contain pathogenic bacteria or organisms that may harm human health. Coliform is one ofbacteria that may cause disease in humans and being used as indicator bacteria of pathogenic bacteria. This research was aimed to know the MPN value number of coliform bacteria and to identify the bacterial presence of genus Escherichia in iced squeezed orange drinks sold in Pontianak City. The sampling used random sampling method. MPN method was used in order to know the number of coliform in iced squeezed orange drinks in Pontianak City. MPN test result obtained the lowest index value of MPN/mL was 0,14/mL in sample in subdistrict Pontianak City and the highest index value in four subdistricts in Pontianak City was MPN/mL >24,00/mL. It was showed that five samples exceed normal limits in accordance with SNI standards SNI 01-3839-1995 based on Permenkes No 492/MENKES/per/IV/2010. Identification resultshowed the bacterial genus of coliform obtained was member of genus Escherichia.

scholarly journals Uji Bakteriologis pada Minuman Air Tebu yang Dijual di Pinggiran Jalan Khatib Sulaiman Kota Padang

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Dewi Oktavia Djasmi ◽  
Roslaili Rasyid ◽  
Eliza Anas
Keyword(s):  
Sugar Cane ◽  
Pathogenic Bacteria ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Index Method ◽  
Probable Number ◽  
E Coli ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Mpn Test

Abstrak Higinitas penjual dan sanitasi lingkungan yang kurang bersih memungkinkan minuman tebu terkontaminasi oleh bakteri patogen. Lokasi penjualan minuman tebu pinggiran jalan yang terbanyak terletak di jalan Khatib Sulaiman kota Padang. Tujuan penelitian ini adalah menentukan kualitas minuman tebu apakah sesuai dengan persyaratanmikrobiologi.  Penelitian ini bersifat deskriptif dengan menggunakan metode indeks Most Probable Number (MPN) di bagian Mikrobiologi Fakultas Kedokteran Universitas Andalas Padang. Sampel pada penelitian ini adalah seluruh penjual minuman tebu yang ada, yaitu sepuluh minuman tebu yang berasal dari lima pedagang. Sepuluh sampel terdiridari lima minuman tebu yang dicampur es dan lima minuman tebu yang tidak dicampur es. Pemeriksaan MPN yang telah dilakukan terdiri dari dua tes, yaitu: tes presumtif dan tes konfirmatif. Hasilnya adalah seluruh sampel positif mengandung bakteri Coliform dan E. coli. Kesimpulan dari penelitian ini adalah air tebu yang dijual di pinggiran jalan Khatib Sulaiman Padang belum memenuhi standar kelayakan konsumsi secara bakteriologis dan terdapat perbedaan indeks MPN antara minuman tebu yang dicampur es dengan yang tidak dicampur es.Kata kunci: tes MPN, air tebu, hygiene Abstract Pathogenic bacteria can contaminate the sugar cane juice by the less hygiene and environmental sanitation of the sellers. There are a lot of people selling sugar cane juice in Padang, mostly in Khatib Sulaiman. The objective of this study was to determine the quality of the sugar cane juice accordance to microbiological requirements. This descriptive study was using the most probable number (MPN) index method and conducted in Microbiology Laboratory of Medical Faculty, Andalas University Padang.The sample in this study is all of sellers, which is ten cane juices fromfive sellers. Ten samples consists of five sugar cane water with ice and sugar cane water without ice MPN test was done by two tests, the presumptive test and the confirmative test. The result showed that all samples contained coliform bacteria and E.coli. The conclusion of this study is sugarca ne juice which is sold on roadside of Khatib Sulaiman is not worthy to microbiological standards for consumption and there is a difference between the MPN index sugar cane water with ice and sugar cane without ice.Keywords: MPN test, cane juice, hygiene

scholarly journals Angka Paling Mungkin (Most Probable Number/MPN) Coliform Sampel Minuman Lidah Buaya Di Pontianak

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Dian Purnama Sari Rahmawati, Elvi Rusmiyanto P.W
Keyword(s):  
Drinking Water ◽  
Pathogenic Bacteria ◽  
Aloe Vera ◽  
Most Probable Number ◽  
Test Results ◽  
Probable Number ◽  
Normal Limits ◽  
The Republic ◽  
Index Value

Aloe vera drink is one of the processed drinks made from the aloe vera leaves, washed until the mucus is reduced and boiled with water. Good drinking water should not contain pathogenic bacteria or organisms that can endanger human health. Coliform is one of the bacteria that can cause disease in humans and is used as an indicator of the presence of pathogenic bacteria. This study aimed to find out the most probable number (MPN) Coliform bacterial in aloe vera drinks which has sold at the location of the sale on Budi Utomo Street, Pontianak City. Sampling used the random sampling method. The MPN test results obtained the lowest MPN/mL index value of 0.091/mL in the samples purchased at the minimarket, while the seven samples obtained at the sales location showed the highest MPN/mL index value of> 24.00/mL. It showed that the eight samples reached over the normal limits listed in SNI 01-3839-1995 based on the Republic of Indonesia Ministry of Health No. 907 / MENKES / SK / VII / 2002 concerning the requirements and supervision of the quality of drinking water which is not more than 0/100 ml.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

scholarly journals Kualitas Air Sumur Gali Kelurahan Lubuk Buaya Kecamatan Koto Tangah Kota Padang Berdasarkan Indeks Most Probable Number (MPN)

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Randa Novalino ◽  
Netti Suharti ◽  
Arni Amir
Keyword(s):  
Water Quality ◽  
Health Department ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Well Water ◽  
Health Minister ◽  
Probable Number ◽  
Sources Of Pollution ◽  
Dug Well ◽  
Mpn Test

AbstrakDiare merupakan salah satu penyakit yang ditularkan melalui air terkontaminasi oleh agen penyebab seperti bakteri Coliform.  Menurut data Dinas Kesehatan Kota (DKK) Padang pada tahun 2011, kejadian diare di Kelurahan Lubuk Buaya Kecamatan Koto Tangah, Kota Padang merupakan kasus tertinggi di Kota Padang. Tujuan penelitian ini adalah menentukan kualitas air sumur gali di Kelurahan Lubuk Buaya berdasarkan Indeks Most Probable Number (MPN) menurut Peraturan Menteri Kesehatan Republik Indonesia (Permenkes RI) No. 416 tahun 1990. Sampel penelitian ini adalah  air sumur gali yang digunakan di beberapa Rukun Tetangga (RT), yang diambil secara acak dari beberapa Rukun Warga (RW) yang telah dipilih sebelumnya, sehingga didapatkan 15 sampel. Penelitian ini dilaksanakan dalam 2 tahap yaitu pengambilan sampel air sumur gali sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologi dengan metode Most Probable Number (MPN) Test. Tes ini terdiri dari tes presumtif dan tes konfirmatif yang disesuaikan dengan Permenkes RI. Hasil penelitian ialah 73,33% dari jumlah sumur yang diperiksa tidak memenuhi standar Permenkes R.I. karena mengandung Coliform > 50 pada setiap 100 ml air. Hanya 26,6% sumur yang memenuhi standar yang telah ditetapkan. Beberapa faktor yang dapat mempengaruhi yaitu lokasi sumber pencemaran, dinding parapet, drainase, tutup sumur dan sarana pengambilan air.Kata kunci: kualitas air sumur gali, MPN, coliform AbstractDiarrhea is one of the diseases that  transmitted through contaminated water by causative agent, one of which is coliform bacteria. According to data from City Health Department Padang in 2011, the incidence of diarrhea in Kelurahan Lubuk Buaya Kecamatan Koto Tangah – Padang is the highest case in the city of Padang. The objective of this study was to determine the water quality of wells dug in Kelurahan Lubuk Buaya by Most Probable Number Index (MPN) according regulation of Indonesian health minister. The 15 samples was water of dug well  in some of the RT households from several neighborhoods that was selected. The research was conducted in two stages, dug well water samples as well as observation of the factors that affect water quality and microbiological examination of the Most Probable Number method (MPN) Test. This test consists of presumptive tests and confirmative tests that were tailored to regulation of Indonesian health minister.  The result were 73.33% of the wells tested did not meet the standards of Indonesian health minister regulation, because it contains >50 coliform in every 100 ml of water. Only 26.6% of the wells were inspected to meet the standards set. Several factors can affect the location of sources of pollution, parapet walls, drainage or sewer water, cover the wells, and water collection facilities.Keywords: dug well water quality, MPN, coliform

scholarly journals Contamination of Escherichia coli Drinking Water Refills on Drinking Water Depots in Malang City

2019 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Wiwik Kusmawati ◽  
Lia Rahayu
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
The City ◽  
Maximum Limit

Data from the Malang City Health Office shows the increasing number of refill drinking water depots in the city of Malang until 2013 there were 163 drinking water depots scattered in each Malang Subdistrict. The purpose of this study was to determine the contamination of Escherichia coli refill drinking water in drinking water depots in Malang. The samples taken were 20 refill drinking water depots in Malang. Sampling is done by simple random sampling. Escherichia coli examination using the Most Probable Number Test. Of the 20 samples tested, 2 samples met the requirements of drinking water quality according to PERMENKES and 18 other samples containing the number of coliform bacteria exceeding the maximum limit of 0 per 100 ml of water. There were Escherichia coli in 10 samples while 8 other samples contained other coliform bacteria.

IDENTIFIKASI BAKTERI Escherichia coli PADA MINUMAN TEH KEMASAN INDUSTRI RUMAH TANGGA DI KELURAHAN SUNGAI DAMA DAN SELILI MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN)

2017 ◽  
Vol 3 (1) ◽  
pp. 59
Author(s):  
Fitri Handayani ◽  
Reksi Sundu ◽  
Dawia Dawia
Keyword(s):  
Escherichia Coli ◽  
Fast Food ◽  
Sampling Method ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
The Public ◽  
Descriptive Research ◽  
Escherichia Coli Bacteria ◽  
Beverage Packaging

Tea beverage packaging is a popular fast food beverage because it has a variety of flavors that are in great demand by the public. Presentation of packaged tea beverages is mostly found in roads that may be contaminated by Escherichia coli bacteria. This study aims to identify Escherichia coli bacteria in packaged tea drinks circulating in the rivers of Kelurahan Selili. This research is a descriptive research. The sample used is packaged tea drink taken in two subdistricts of Samarinda ilir namely Selili urban village and Sungai Dama by using Purposive Sampling method of 10 samples. Sample tests include presumtive test, confirmative test and completed test. The LB (Lactose Broth) assay test was used to detect early presence of Coliform bacteria, BGLBB (Brillian Green Lactose Bile Broth) as a detector of the specification of the presence of Coliform bacteria and completed test using EMBA (Eosin Methylene Blue Agar) as Escherichia coli. The results of this study were 7 samples of 10 samples tested containing Escherichia coli.

scholarly journals Microbial Content Test on Sliced Papaya in Area of Universitas Sumatera Utara

2020 ◽  
Vol 3 (2) ◽  
pp. 86-92
Author(s):  
Nenni Dwi Aprianti Lubis ◽  
Sri Amelia ◽  
Ridwan Balatif
Keyword(s):  
Laboratory Tests ◽  
Sampling Method ◽  
High Water ◽  
Most Probable Number ◽  
High Water Content ◽  
Probable Number ◽  
Biochemical Identification ◽  
Pathogenic Microbes ◽  
The University ◽  
Mpn Test

Papaya is a fruit that is often consumed in Indonesia and has increased consumption from 2015-2016 based on SUSENAS 2016. Papaya has high water content (85-90%) and the pH is near neutral. This makes papaya vulnerable and suitable medium for the growth of pathogenic microbes. The presence of pathogenic microbes in papaya can cause health problems for those who consumed them. Objectives this study was to observe the microbial content of sliced papaya in the Universitas Sumatera Utara. Method this research was carried out by conducting laboratory tests and the results obtained in the form of a description of microbial content in papaya cut samples. The content of coliform microbes in sliced papaya was tested using the Most Probable Number (MPN) method and biochemical identification. Papaya samples were selected using total sampling method from the University of Sumatera Utara. Results A total of 14 sliced papaya samples were analysed in this study. By using the MPN test it was found that all papaya samples had a microbial threshold exceeding those determined by SNI 7388: 2009 which is below <20 / g. A total of 9 papaya samples (64.28%) contained Klebsiella sp. bacteria, 5 samples (35.72%) contained E.coli bacteria. Conclusions Bacteria found in this study were Escherichia coli and Klebsiella sp. The presence of microbial content in the sliced papaya sample that exceeds the threshold must be a concern of various parties in the surveillance of snacks being sold.

scholarly journals Analisis Bakteri Coliform Pada Air Minum Isi Ulang Di Wilayah Poasia Kota Kendari

2017 ◽  
Vol 13 (2) ◽  
pp. 71-76
Author(s):  
Askrening Askrening ◽  
Reni Yunus
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Sampling Method ◽  
Treatment Process ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Safe Drinking Water ◽  
Probable Number ◽  
Water Treatment Process ◽  
Alternative Water

The existence of safe drinking water now begin to be limited in number, so that the existence of refill drinking water depotbecomes an alternative water treatment process, in principle, should be able to eliminate all kinds of pollutants, includingcoliform bacteria, which is a group of bacteria used as an indicator of pollution, waste and conditions which are not good forwater. This study aims to determine coliform bacteria contamination in refill drinking water depot in Poasia Kendari. The typeof research is descriptive analytic approach using MPN (Most Probable Number). These samples included 10 samples takenwith saturated total sampling method. Data were analyzed by using frequency distribution. The results showed that thesamples were positive in getting as much as 6 samples (60%) and over the limit contamination and negative samples ingetting as many as four samples (40%), so it can be concluded that from coliform bacteria identification of 10 samples of refilldrinking water in Poasia Kendari, 6 samples were identified contaminated with coliform bacteria and microbial contaminationwas over the limit of PERMENKES 492 / Menkes / Per / IV.2010.

scholarly journals Microbial Contamination Analysis of Mobile Phones from certain Users of Chattogram, Bangladesh

2021 ◽  
Vol 14 (4) ◽  
pp. 1550-1556
Author(s):  
Mohammad Zobaidul Alam
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Coliform Bacteria ◽  
Personal Hygiene ◽  
Diffusion Method ◽  
Most Probable Number ◽  
Probable Number ◽  
Hospital Patients

Mobile phone is an essential part of everyday life in modern days. Mobile phones act as vehicles for transmitting pathogenic bacteria due to lack of awareness and widespread use. This study aimed to investigate the bacterial contamination of mobile phones of different categories people at Chattogram city, Bangladesh. During the present study, 40 swab samples were collected from the mobile phones of students, businessmen, fishermen, and hospital patients for the isolation, identification of mobile phone associated bacteria, and their antibiogram. In our study, total viable count (TVC) was performed by the pour plate method and total coliform count (TCC) by the most probable number (MPN) method. Besides these, five selective media were used to isolate pathogenic bacteria from mobile phones and then identified. Antibiotic sensitivity assay was performed by disc diffusion method with 10 different antibiotics. Mobile phones of hospital patients (20165 cfu/ml) and students (1578 cfu/ml) showed the highest and lowest TVC respectively. Coliform bacteria were detected from the mobile phones of 100% hospital patients, 90% from both businessmen, and fishermen but only 30% from students. Klebsiella pneumoniae and Pseudomonas aeruginosa were found the most prevalent bacteria but Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus sp., Bacillus sp., E. coli, Salmonella sp., Citrobacter sp., Serratia sp., Proteus sp., and Enterobacter sp. were also detected. Almost all the isolates were highly resistant to ampicillin, amoxicillin, rifampin, erythromycin and sensitive to ciprofloxacin, gentamicin, azithromycin, and tetracycline. Our findings ensure that mobile phones act as an important source of pathogenic organisms for humans and can serve as a vehicle for cross-transmission of microbiota. So, washing hands before and after handling food and also personal hygiene is very important.

Analisis Jumlah Coliform dan Faecal Coli (MPN) pada Daging Sapi dan Ayam di Kota Manokwari

2020 ◽  
Vol 10 (2) ◽  
pp. 113
Author(s):  
Abdul Rahman Ollong ◽  
John Arnold Palulungan ◽  
Rizki Arizona
Keyword(s):  
Broiler Chicken ◽  
Pathogenic Bacteria ◽  
Microbial Growth ◽  
Chicken Meat ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Cross Contamination ◽  
Eligibility Criteria ◽  
Probable Number ◽  
Traditional Market

Abstract Beef and chicken meat are potential as a medium for microbial growth such as coliform bacteria since it has a high nutrients content. Coliform bacteria in a given amount could be used as a hygienic indicator and a sign for the presence of pathogenic bacteria. The aimed of this research was to determine the amount of coliform and Escherichia coli on fresh beef and broiler chicken. The bacterial tests included qualitative tests as presumptive test, confirmed test, completed test whereas quantitavely test as Most Probable Number (MPN) to test the number of coliform and faecal coli. The results showed that pH in beef meat is normal and chicken meat shows under normal pH (pH in beef anf chicken meat are  5,7 – 5,98).   Coliform and faecal coli bacteria was detected in 100% of samples had MPN values > 1 MPN/g. It means that the value do not meet eligibility criteria consumption. The high number of Coliform and faecal coli contamination in beef and chicken meat were supposed in relation with market sanitation, cross contamination with other food, low knowledge about hygiene.  Keywords: Number of coliform; Beef and chicken; Traditional market   Abstrak Daging sapi dan ayam memiliki kandungan nutrisi yang tinggi sehingga menjadi media yang baik untuk pertumbuhan mikroba. Salah satu jenis bakteri yang sering mengkontaminasi yaitu coliform dan faecal coli yang merupakan indikator higienis dan sebagai tanda keberadaan bakteri patogen. Penelitian ini bertujuan untuk menghitung jumlah coliform dan faecal coli pada sampel daging sapi dan ayam yang dijual di pasar traditional kota Manokwari menggunakan uji kualitatif yaitu uji presumptive test, confirmed test, completed test sedangkan kuantitatif yaitu Most Probable Number (MPN) untuk menguji jumlah coliform dan faecal coli. Hasil penelitian menunjukkan bahwa nilai pH daging sapi dalam kisaran normal dan pH ayam tergolong rendah yang semuanya pada kisaran 5,7 – 5,98; dan  100% sampel daging sapi, ayam dan pedagang daging terkontaminasi bakteri coliform dan faecal coli dengan nilai MPN yang tidak memenuhi kriteria kelayakan konsumsi, yakni > 1 MPN/g. Tingginya cemaran coliform dan faecal coli pada daging sapi dan ayam terkait dengan faktor kualitas sanitasi pasar, kontaminasi silang dengan bahan makanan lain dan pengetahuan higienitas yang rendah. Kata kunci : Jumlah coliform dan faecal coli; Daging sapi dan ayam; Pasar Tradisional

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