scholarly journals Uji Cemaran Mikroba Pada Air Yang Digunakan Untuk Mencuci Peralatan Makan Oleh Pedagang Kaki Lima di Sekitar UIN Sunan Ampel Surabaya

2021 ◽  
Vol 5 (2) ◽  
pp. 101-115
Author(s):  
Fahimatul Ula ◽  
Misbakhul Munir ◽  
Hanik Faizah
Keyword(s):  
Escherichia Coli ◽  
Water Sample ◽  
Plate Count ◽  
Most Probable Number ◽  
Food Hygiene ◽  
Street Vendors ◽  
Probable Number ◽  
Total Plate Count ◽  
Before And After ◽  
Mpn Test

The condition of eating places such as street vendors will determine the level of food hygiene, including the water used to wash food utensils. This study aimed to determine the number of microbes and identify Escherichia coli and Salmonella sp. on the water that was used to wash tableware by street vendors around UIN Sunan Ampel Surabaya. Water samples before and after being used to wash tableware were obtained from 4 street vendors. The number of microbes in the sample was tested by the Total Plate Count (TPC) and Most Probable Number (MPN) methods, Escherichia coli was identified using Eosin Methylene Blue Agar (EMB) media, and Salmonella sp. was identified using Salmonella-Shigella agar (SSA) and Triple Sugar Iron agar (TSIA) media. Based on the results of TPC test, the water sample before being used to wash tableware had the lowest and highest values that were 3.0 x 102 Cfu/ml and 9.8 x 105 Cfu/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 4.0 x 102 Cfu/ml and 3.5 x 105 Cfu/ml, respectively. In the MPN test, the water sample before being used to wash tableware had the lowest and highest values that were 15/100ml and 26 100/ml, respectively, while the water sample after being used to wash tableware had the lowest and highest values that were 72/100 ml and 271/ 100 ml, respectively. Escherichia coli was detected in all samples 1&3 while in sample 2 Escherichia coli was detected in the water before it was detected the genus Enterobacter and in samples 4 the genus Enterobacter was detected and all samples water used to wash tableware of street vendors was positive for Salmonella.

scholarly journals Penilaian Kualitas Air Minum Produk Smart Water Station Berdasarkan Parameter Mikrobiologi Menggunakan Metode Most Probable Number di Fakultas Kedokteran UKRIDA

2019 ◽  
Vol 25 (2) ◽  
pp. 66-74
Author(s):  
Donna Mesina Rosadini Pasaribu ◽  
Fransiska Elviana Arly ◽  
Wani Devita Gunadi
Keyword(s):  
Escherichia Coli ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Smart Water ◽  
Total Plate Count

Kebutuhan air yang paling utama dalam kehidupan manusia tidak terlepas dari air bersih terutama sebagai air minum. Namun, tidak semua air yang ada di bumi dapat dikonsumsi oleh manusia karena air juga merupakan media yang baik untuk kehidupan bakteri. Menurut Peraturan Menteri Kesehatan (Permenkes) No. 492/MENKES/PER/IV/2010 tentang persyaratan kualitas air minum untuk seluruh penyelenggara bahwa air minum tidak boleh mengandung bakteri patogen yang dapat menyebabkan penyakit, terutama penyakit saluran pencernaan yaitu bakteri Coliform dan Escherichia coli. Kadar maksimum kandungan bakteri Coliform dan Escherichia coli dalam air minum adalah 0 per 100 ml sampel. Penelitian ini bertujuan untuk menganalisis kualitas air minum produk Smart Water Station yang ada di Fakultas Kedokteran Universitas Kristen Krida Wacana (UKRIDA). Penelitian ini dilakukan di Laboratorium Mikrobiologi Kampus II UKRIDA pada sampel yang telah memenuhi kriteria inklusi dan eksklusi. Jenis penelitian ini adalah eksperimental deskriptif yang  diperoleh melalui observasi laboratorium berdasarkan parameter fisik dan mikrobiologi dengan menggunakan Most Probable Number (MPN) dan Total Plate Count (TPC). Hasil penelitian menunjukkan bahwa tidak ada satupun sampel melebihi batas maksimum yang  dipersyaratkan dalam air minum, serta berdasarkan parameter fisik baik rasa, bau, maupun suhu berada dalam batas normal.  

A New Medium for Determining the Total Plate Count in Food

1999 ◽  
Vol 62 (12) ◽  
pp. 1404-1410 ◽  
Author(s):  
C. F. SMITH ◽  
D. E. TOWNSEND
Keyword(s):  
Linear Regression Analysis ◽  
Alternative Methods ◽  
Plate Count ◽  
Most Probable Number ◽  
Suitable Alternative ◽  
Probable Number ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Standard Plate

SimPlate for Total Plate Count–Color Indicator (TPC-CI, IDEXX Laboratories, Inc., Westbrook, Me.) is a new medium that incorporates the redox dye resazurin to detect and quantify bacteria in food. Enumeration is achieved by the most probable number method using a SimPlate device. Viable bacteria are detected in each well of the SimPlate device by the biochemical reduction of resazurin, which is blue, to the pink resorufin or the clear dihydroresorufin indicators. Results after 24 h of incubation for TPC-CI are highly correlated with standard plate count agar after 48 h of incubation. Correlation coefficients from studies conducted at five laboratories ranged from 0.94 to 0.98 in side-by-side comparisons against standard plate count agar. Four additional test sites, using alternative methods for determining the aerobic plate count in food, reported similar results in comparison studies (r = 0.91 to 0.97). The slopes from linear regression analysis at all sites ranged from 0.91 to 0.98, with y intercepts ranging from 0.11 to 0.84. Samples used for the validation of TPC-CI included raw food products (i.e., liver and grains), which may contain natural enzymes that interfere with enzyme-based detection methods. No interference was seen from the foods tested. These results suggest that TPC-CI is a suitable alternative to existing plate count methods and has reduced incubation time.

Identifikasi Kualitas Air Sumur, Air dalam Kemasan, dan Air Sungai dengan Metode Most Probable Number

2021 ◽  
Vol 10 (1) ◽  
pp. 40-45
Author(s):  
Ni Putu Trisna Ayundita
Keyword(s):  
Escherichia Coli ◽  
Most Probable Number ◽  
Probable Number ◽  
Mpn Test

Air memiliki peranan yang sangat besar salah satunya untuk kebutuhan biologisnya, yaitu bertahan hidup. Masalah utama yang dihadapi berkaitan dengan sumber daya air adalah kualitas air. Penentuan kualitas air secara mikrobiologi dilakukan dengan Most Probable Number (MPN) Test. Penelitian ini menggunakan 3 sampel yaitu air sumur, air dalam kemasan, dan air sungai. Air sumur mengandung Coliform dan Escherichia coli dengan jumlah yang sama yaitu 14 MPN/100 mL. Pada uji dugaan, air dalam kemasan menunjukkan hasil positif, namun saat diuji penetapan diperoleh hasil negatif. Sedangkan pada air sungai mengandung bakteri coliform lebih dari 1100 MPN/ 100 mL dan Escherichia coli sebanyak 11 MPN/ 100 mL.  Sampel air minum dalam kemasan layak dikonsumsi sedangkan untuk sampel air sumur dan air sungai tidak layak dikonsumsi karena mengandung Coliform dan Escherichia coli.

Enumeration of Total Aerobic Bacteria and Escherichia coli in Minced Meat and on Carcass Surface Samples with an Automated Most-Probable-Number Method Compared with Colony Count Protocols

2006 ◽  
Vol 69 (10) ◽  
pp. 2500-2503 ◽  
Author(s):  
P. PAULSEN ◽  
E. SCHOPF ◽  
F. J. M. SMULDERS
Keyword(s):  
Escherichia Coli ◽  
Bacterial Flora ◽  
Colony Count ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Plate Count Agar ◽  
Two Samples ◽  
Minced Meat

An automated most-probable-number (MPN) system for the enumeration of total bacterial flora and Escherichia coli was compared with plate count agar and tryptone-bile-glucuronide (TBX) and ColiID (in-house method) agar methodology. The MPN partitioning of sample aliquots was done automatically on a disposable card containing 48 wells of 3 different volumes, i.e., 16 replicates per volume. Bacterial growth was detected by the formation of fluorescent 4-methylumbilliferone. After incubation, the number of fluorescent wells was read with a separate device, and the MPN was calculated automatically. A total of 180 naturally contaminated samples were tested (pig and cattle carcass surfaces, n = 63; frozen minced meat, n = 62; and refrigerated minced meat, n = 55). Plate count agar results and MPN were highly correlated (r = 0.99), with log MPN =−0.25 + 1.05·log CFU (plate count agar) (n = 163; range, 2.2 to 7.5 log CFU/g or cm2). Only a few discrepancies were recorded. In two samples (1.1%), the differences were ≥1.0 log; in three samples (1.7%), the differences were ≥0.5 log. For E. coli, regression analysis was done for all three methods for 80 minced meat samples, which were above the limit of detection (1.0 log CFU/g): log MPN = 0.18 + 0.98·log CFU (TBX), r = 0.96, and log MPN =−0.02 + 0.99·log CFU (ColiID), r = 0.99 (range, 1.0 to 4.2 log CFU/g). Four discrepant results were recorded, with differences of >0.5 but <1.0 log unit. These results suggest that the automated MPN method described is a suitable and labor-saving alternative to colony count techniques for total bacterial flora and E. coli determination in minced meat or on carcass surfaces.

Microbial Hazards Associated with Bean Sprouting

1982 ◽  
Vol 65 (2) ◽  
pp. 241-248 ◽  
Author(s):  
Wallace H Andrews ◽  
Philip B Mislivec ◽  
Clyde R Wilson ◽  
Verneal R Bruce ◽  
Paul L Poelma ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Before And After ◽  
Alternaria Species ◽  
Aerobic Plate Count ◽  
Yeasts And Molds ◽  
Mold Count ◽  
Total Yeast

Abstract The behavior of microorganisms was studied in mung beans and alfalfa seeds before and after germination in modified, commercially available bean-sprouting kits. The microorganisms were enumerated by the aerobic plate count (APC) and by total yeast and mold count procedures. Salmonella species were artificially inoculated into selected samples and were enumerated by the most probable number (MPN) method. After germination of the beans or seeds into mature sprouts, significant increases were noted in APCs and in MPN values of Salmonella species. Although counts of yeasts and molds did not increase significantly after germination, these samples showed an increase in toxic Aspergillus flavus and potentially toxic Alternaria species. The presence of toxic Penicillium cyclopium molds also increased substantially in 5 samples of a single brand of mung beans. Analysis of selected sprout samples, however, showed no presence of aflatoxin.

Comparison of a Rapid Plate Count and MPN Methods for Enumeration of Fecal Coliforms and Escherichia coli in Soft-Shell Clams

1995 ◽  
Vol 58 (11) ◽  
pp. 1197-1200 ◽  
Author(s):  
GUADALUPE R. GARCIA ◽  
ROBERT E. HAYMOND ◽  
DIANE M. SPRAGUE ◽  
EMMA R. SINGLETON ◽  
JAMES T. PEELER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Mya Arenaria ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Rapid Procedure ◽  
Soft Shell ◽  
Plate Count Method

A direct elevated temperature plate count method utilizing modified fecal coliform agar with rosolic acid (ETPC/mFC) was compared to 5-tube and 3-tube most probable number (MPN) procedures for its accuracy in enumerating fecal coliforms and Escherichia coli in naturally and artificially contaminated soft-shell clams (Mya arenaria). The results indicated that the extent of overall recovery of fecal coliforms was similar in the two methods tested. Therefore, the ETPC/mFC method may be considered as a rapid procedure for fecal coliform screening during depuration of soft-shell clams.

Comparison of the SimPlate™ Total Plate Count Method with Petrifilm™, Redigel™, and Conventional Pour-Plate Methods for Enumerating Aerobic Microorganisms in Foods

1998 ◽  
Vol 61 (1) ◽  
pp. 14-18 ◽  
Author(s):  
L. R. BEUCHAT ◽  
F. COPELAND ◽  
M. S. CURIALE ◽  
T. DANISAVICH ◽  
V. GANGAR ◽  
...  
Keyword(s):  
Food Samples ◽  
Test Methods ◽  
Plate Count ◽  
Most Probable Number ◽  
Suitable Alternative ◽  
Probable Number ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Wide Range ◽  
Highly Correlated

The SimPlate™ Total Plate Count (TPC) method, developed by IDEXX Laboratories, Inc., is designed to determine the most probable number of aerobic microorganisms in foods. The 24-h test was compared to the conventional plate count agar (PCA) method, the Petrifilm™ Aerobic Count plates, and the Redigel™ Total Count procedure for enumerating microflora in 751 food samples. Results using the SimPlate™ TPC method were highly correlated (r ≥ 0.96) with results from other test methods. Slopes (0.96–0.97) were not significantly different from 1, and y intercepts (−0.03–0.08) were not different from 0. The SimPlate™ has a high counting range (> 1600 most probable number per single dilution), thus requiring fewer dilutions of samples compared to other methods evaluated. Some foods, e.g., raw liver, wheat flour, and nuts, contain enzymes that gave false-positive reactions on SimPlates™. Overall, however, the SimPlate™ TPC method is a suitable alternative to conventional PCA, Petrifilm™, and Redigel™ methods for estimating populations of mesophilic aerobic microorganisms in a wide range of foods.

scholarly journals Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi

2021 ◽  
Vol 4 (2) ◽  
pp. 207
Author(s):  
Achmad Arby Wijaya ◽  
Iwan Sahrial Hamid ◽  
Maya Nurwartanti Yunita ◽  
Wiwiek Tyasningsih ◽  
Ratih Novita Praja
Keyword(s):  
Escherichia Coli ◽  
Total Sample ◽  
Cow Milk ◽  
Most Probable Number ◽  
Methyl Red ◽  
Probable Number ◽  
E Coli ◽  
Fresh Milk ◽  
Peptone Water ◽  
Mpn Test

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.

scholarly journals Mutu Bakteriologis Saus Tomat Pentol di Banjarbaru

2016 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Ratih Dewi Dwiyanti ◽  
Leka Lutpiatina
Keyword(s):  
Staphylococcus Aureus ◽  
Plate Count ◽  
Most Probable Number ◽  
Chemical Parameters ◽  
Probable Number ◽  
Tomato Sauce ◽  
Total Plate Count ◽  
Dressing Materials ◽  
And Staphylococcus Aureus

<p style="text-align: justify;">Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.

Comparison of culturable fecal coliforms and Escherichia coli enumeration in freshwaters

2007 ◽  
Vol 53 (6) ◽  
pp. 798-801 ◽  
Author(s):  
Tamara Garcia-Armisen ◽  
Josué Prats ◽  
Pierre Servais
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Quality Data ◽  
Plate Count ◽  
Most Probable Number ◽  
Tetrazolium Chloride ◽  
Probable Number ◽  
E Coli ◽  
Glucuronidase Activity

Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detection of glucuronidase activity. FC and E. coli counts were highly correlated; an average E. coli/FC ratio equal to 0.77 was found, meaning that on average, 77% of FC were E. coli. Knowing the E. coli/FC ratio allows us to convert the historical microbiological quality data expressed in FC counts into E. coli abundance and thus to compare with present and future monitoring data that are (or will be) based on E. coli enumeration.

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