Background:
N’,N’-diethyl-m-toluamide (DEET) is the most widely used repellent substance worldwide. It is
formulated as aerosol, solution, lotion, gel and patches. However, the official compendia report monographs to analyze
only DEET drug substance and solution.
Objective:
In this study an isocratic HPLC method was validated to assay DEET in lotion, gel and solution, under the
same analytical conditions.
Method:
The method was validated according to ICH requirements and DEET detection was achieved at around 11 min,
using C-18 column, a mobile phase composed by methanol, acetonitrile and water pH 4.5 (45:10:45), flow rate at 1 mL
min-1 and detection at 270 nm.
Results:
A linear relationship was observed in the range of 2.5 to 100 µg mL-1, the method was precise (relative standard
deviation < 2%) and accuracy was demonstrated by DEET recovery values ranging from 99.5 to 100.2%. The specificity
was studied by a forced degradation test, where degradation products were observed after alkaline degradation and
ultraviolet radiation. Appropriate resolution between DEET, degradation products and excipient peaks indicated the
method specificity. Robustness was evaluated by a full factorial design, and no effect on DEET assay was observed under
simultaneous variation in analytical parameters. The method was applied to assay nine marketed formulations,
demonstrating its good applicability.
Conclusion:
The validated HPLC method was successfully applied to the quantitative analysis of DEET in lotion, gel and
solution, contributing to improve the quality control and the efficacy of these formulations.