Bio-Crude Production through Recycling of Pretreated Aqueous Phase via Activated Carbon

The management and optimization of the aqueous phase are the major challenges that hinder the promotion of hydrothermal liquefaction (HTL) technology on a commercial scale. Recently, many studies reported about the accumulation of the N-content in the bio-crude with continuous recycling of the aqueous phase from high protein-containing biomass. In the present study, sewage sludge was processed at 350 °C in an autoclave. The produced aqueous phase was treated with activated carbon, and its subsequent recycling effect on the properties of the bio-crude and aqueous phase was investigated. By contacting the aqueous phase with activated carbon, 38–43% of the total nitrogen was removed from the aqueous phase. After applying the treated aqueous phase recycling, the energy recovery of the bio-crude increased from 50 to 61% after three rounds of recycling. From overall carbon/nitrogen recoveries, 50 to 56% of the carbon was transferred to the bio-crude phase and more than 50% of the nitrogen remained in the aqueous phase. The aqueous phase contained mostly of N&O-heterocyclic compounds, small chain organic acids, and amides. ICP-AES analysis showed that more than 80% of the inorganic elements were concentrated into the solid phase.

RNA-Seq Transcriptome Analysis of Potato with Differential Tolerance to Bentazone Herbicide

Potato (Solanum tuberosum), an important food crop worldwide, is threatened by broadleaf weeds. Bentazone is an effective herbicide for controlling weeds; however, as a photosynthesis inhibitor, it can also affect potato plants. Therefore, screening potato seedlings for bentazone resistance and determining the genes involved is essential. Herein, we selected potato varieties with tolerance and sensitivity to bentazone. The photosynthetic rate of sensitive plants was notably affected by bentazone application, whereas the tolerant plants showed a significantly higher photosynthetic rate. We observed 95.7% bentazone degradation within 24 d after application in the tolerant plants. Transcriptome sequencing revealed that the numbers of differentially expressed genes (DEGs) between the tolerant and sensitive potato seedlings were 2703 and 11,024 before and after bentazone application, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the majority of DEGs were enriched in metabolic pathways, biosynthesis of secondary metals, carbon metabolism, glutathione metabolism, and photosynthesis. Polyphenol oxidase (PPO), flavonoid 3’,5’-methyltransferase-like (AOMT3), ribulose bisphosphate carboxylase small chain C (RBCS-C), and chalcone synthase 2 (CHS2) were identified as candidates contributing to bentazone tolerance. These results provide a theoretical basis for selecting potato stress-resistant resources in the future.

PSIII-19 The Effect of Xylanase on Finisher Pig Performance

The breakdown of long chain arabinoxylans into smaller chain xylo-oligomers by the use of xylanase results in a shift towards fiber utilizing bacteria resulting in production of small chain fatty acids and improved pig performance. The objective of this study was to evaluate the effect of xylanase (Econase XT; AB Vista) on finisher pig performance. A total of 598 pigs; (37.4 ± 1.0 kg) were fed a single-phase diet for 60 days. Pigs were assigned to two treatments; 0 (CTL) or 16,000 BXU/kg of xylanase (XYL) with 12 pen replicates and 24/25 pigs (mixed sex; gilts and boars) per treatment. The diet was based on wheat, barley, soybean meal, rapeseed meal, and wheat midds; SID Lys: 0.88%, NDF: 14.7%, and NE: 2090 kcal/kg. Average daily gain (ADG), feed intake (ADFI) and feed conversion ratio (FCR) were evaluated in phase 1 (0-26d), phase 2 (26-60d) and overall (0-60d). Liveability was measured per treatment. Data were analysed using JMP 12 using the standard least square platform and ANOVA was performed to determine significance at P < 0.05. The model included initial BW as a covariant. The results indicated that there was no effect of xylanase on liveweight, ADG or ADFI at any phase of the finisher period. The use of xylanase improved FCR in phase 2 (2.53 v 2.60: P < 0.05) and overall (2.44 v 2.49 P=0.03). No differences were seen in liveability (CTL; 96%; XYL 98%; P = 0.29). It can be concluded that the use of xylanase can be used in finisher feeds to improve FCR which is linked with greater energy utilisation that has been associated with greater fiber breakdown through xylanase use.

Types of grocery stores and demographic and household factors in Japanese provincial area

Background Neighborhood grocery stores, such as supermarkets (SM) and convenience stores (CVS), have received attention as a determinant of a food purchase behavior. Previous studies reported that grocery store entry or closure was associated with regional populations. This study examines the association of types of SM (i.e. cross-regional, local, or small chain stores), CVS, and household demographics in the cities, towns and villages (having its own incorporated government) in Hiroshima and Okayama Prefectures, Japan. Methods We hypothesized that the entry and closure of SM, CVS are associated with the regional population and type of family household from interview surveys with supermarket and wholesale operators. We collected data on SM and CVS from Large-Scale Retail Store Data in Japan, while data on demographics and households were collected from the census. Data sets from 59 municipalities (city, town, ward, and village) and 49 SM chains (498 stores) and 8 CVS chains (1235 stores) were analyzed using multiple regression analysis. Results Some household factors showed significant associations with the number of SM and CVS at the municipality level. Those municipalities with a greater number of extended families and single households had significantly more “cross-regional” as well as “local chain SM” stores. Those municipalities with more nuclear families had significantly more “small chain SM” and fewer “local chain SM” stores. Those municipalities with more single households had significantly fewer SM stores. Conclusions In these prefectures, population declining and single household increasing are expected over the coming decades. If no political interventions are allowed on this issue, some of “food desert” may emerge for the hundreds of thousands people living in these areas; elderly people may not get fresh vegetables in their neighborhood, and young parents will have to drive for hours to “cross-regional SM” to buy fresh milk for their children. Key messages Supermarkets and convenience stores are determinant of a food purchase behavior. The association of the number of types of SM, CVS, and demographic and household factors.

What does informal access to misoprostol in Colombia look like? A mystery client methodology in Bogotá and the Coffee Axis

IntroductionIn 2006, abortion was decriminalised in Colombia under certain circumstances. Yet, women avail themselves of ways to terminate pregnancy outside of the formal health system. This study explored how drug sellers engage with women who attempt to purchase misoprostol from them.MethodsA mapping exercise was undertaken to list small-chain and independent drug stores in two regions in Colombia. A sample (n=558) of drug stores was selected from this list and visited by mystery clients between November and December 2017. Mystery clients sought to obtain a medication to bring back a delayed period, and described the experience, the information obtained and the medications proffered in exit interviews.ResultsMisoprostol was offered for purchase in 15% of the visits; in half of visits, only information about misoprostol was shared, while no information about misoprostol was provided on the remaining visits. Over half of sellers who refused to sell any medication provided referrals, most commonly to an abortion provider. Among visits which included discussion of misoprostol, two out of five sellers provided dosage instructions with most recommending the minimum adequate dosage. Mystery clients received little information on the physical effects to expect with the use of misoprostol and possible complications.ConclusionsAs misoprostol is being obtained from some drug sellers without a prescription, capacitating this cadre with at least a minimum of standardised information on dosage, routes of administration and expected effects and outcomes have the potential to improve reproductive health outcomes for women who choose to terminate pregnancies this way in Colombia.

Examining the relationships between market indicators and hotel pricing approaches

Hotels are employing revenue management (RM) to improve profitability by efficiently managing the effects of capacity, as well as the effects of performance factors to model consumer behavior. This empirical paper discusses the extent and use of different pricing approaches and their success in this multichannel environment. Do hotels consider the effect of different pricing strategies? We compare the impact of the RM factors on the main pricing techniques and by using a hotel chain scale. Based on a sample of revenue managers’ responses, the results of this study confirm that while traditional pricing techniques are an old applicable approach, they are still used extensively. The empirical results show an association among distribution channels and dynamic pricing (DP) strategies, albeit the relationship is not such robust concerning traditional pricing techniques. From a practical standpoint, hotels would advance consumer-centric strategies to bargain competitive rates in the market. The results indicate that more than half of the respondent properties transact business through a type of opaque mechanism, but not for the luxury category. Empirical analysis by chain scale illustrates that in practice, most companies are adopting different pricing techniques considering the period and the market hurdles. Small chain hotels mainly implemented some form of traditional pricing techniques or the opaque mechanism, while the DP approach is more consistent with large chain hotels. This implies that the current environment of available data on the pricing optimization left of any provision of the consumer’s willingness to pay is challenging and distresses the hotels’ promotion of product segmentation.

Measuring the Meta Efficiency and Its Determinants on Efficiency in the Korean Coffee Shop Franchise

Previous applications of DEA (data envelopment analysis) models in the field of franchising have been scarce. In particular, measurement and analysis of the operational efficiency of coffee shop franchisors in the explosively growing coffee market in Korea is required. In this study, we categorize 29 Korean coffee shop franchisors into three groups according to their number of franchisees and employ the metafrontier analysis to measure the efficiency of coffee shop franchisors from 2015 to 2018. Based on the results of metafrontier DEA, this study identifies sources of inefficiency within individual coffee shop franchisors to provide insights for coffee franchise operators and investigates efficiency differences between small-chain and medium-chain coffee shop franchisors. Furthermore, the results of bootstrapped truncated regression provide that the external environmental factors affecting the efficiency of each individual group are totally different patterns depending on the franchise group. Based on the analysis results, this study suggests strategic initiatives tailored to individual coffee shop groups to enhance their operating efficiency and identifies the principal operational drivers of the environmental variables to achieve sustainable growth.