Replacement of traditional components of the backfill mixture with man-made waste

The formation and storage of man-made waste of water-soluble ores creates a global environmental problem that entails changing landscapes in mining areas and environmental degradation. The involvement of man-made waste in a closed cycle of the production and technological chain makes it possible to reduce the impact of mining and processing on the environment. The use of non-waste (low-waste) technologies, in addition to reducing the environmental burden, allows you to expand the raw material base of the enterprise by replacing the traditional components of the backfill mixture with man-made waste from mining and processing enterprises. The possibility of replacing the traditional, specially extracted aggregate in the backfill mixture with industrial waste of water-soluble ores is experimentally proved. The possibility of creating a cementless backfill mixture is proved. The possibility of replacing the cement binder with magnesium-containing slags of the Chusovsky Metallurgical Plant was confirmed. It was found that the separate activation treatment of the components of the backfill mixture has a positive effect on its rheological properties and increases the strength of the joint mass. The use of lignosulfonate improves the quality of the mixture and the resulting mass. The development of a backfill composite from man-made waste makes it possible to implement the principle of organizing mining production, which provides for the use of intermediate products in cyclic production and excludes the formation of man-made waste of water-soluble ores.

Flecainide Paradoxically Activates Cardiac Ryanodine Receptor Channels under Low Activity Conditions: A Potential Pro-Arrhythmic Action

Cardiac ryanodine receptor (RyR2) mutations are implicated in the potentially fatal catecholaminergic polymorphic ventricular tachycardia (CPVT) and in atrial fibrillation. CPVT has been successfully treated with flecainide monotherapy, with occasional notable exceptions. Reported actions of flecainide on cardiac sodium currents from mice carrying the pro-arrhythmic homozygotic RyR2-P2328S mutation prompted our explorations of the effects of flecainide on their RyR2 channels. Lipid bilayer electrophysiology techniques demonstrated a novel, paradoxical increase in RyR2 activity. Preceding flecainide exposure, channels were mildly activated by 1 mM luminal Ca2+ and 1 µM cytoplasmic Ca2+, with open probabilities (Po) of 0.03 ± 0.01 (wild type, WT) or 0.096 ± 0.024 (P2328S). Open probability (Po) increased within 0.5 to 3 min of exposure to 0.5 to 5.0 µM cytoplasmic flecainide, then declined with higher concentrations of flecainide. There were no such increases in a subset of high Po channels with Po ≥ 0.08, although Po then declined with ≥5 µM (WT) or ≥50 µM flecainide (P2328S). On average, channels with Po < 0.08 were significantly activated by 0.5 to 10 µM of flecainide (WT) or 0.5 to 50 µM of flecainide (P2328S). These results suggest that flecainide can bind to separate activation and inhibition sites on RyR2, with activation dominating in lower activity channels and inhibition dominating in more active channels.

Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae

Gcr1, an important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently revealed to have two isoforms, Gcr1U and Gcr1S, produced from un-spliced and spliced transcripts, respectively. In this study, by generating strains expressing only Gcr1U or Gcr1S using the CRISPR/Cas9 system, we elucidate differential activation mechanisms of these two isoforms. The Gcr1U monomer forms an active complex with its coactivator Gcr2 homodimer, whereas Gcr1S acts as a homodimer without Gcr2. The USS domain, 55 residues at the N-terminus existing only in Gcr1U, inhibits dimerization of Gcr1U and even acts in trans to inhibit Gcr1S dimerization. The Gcr1S monomer inhibits the metabolic switch from fermentation to respiration by directly binding to the ALD4 promoter, which can be restored by overexpression of the ALD4 gene, encoding a mitochondrial aldehyde dehydrogenase required for ethanol utilization. Gcr1U and Gcr1S regulate almost the same target genes, but show unique activities depending on growth phase, suggesting that these isoforms play differential roles through separate activation mechanisms depending on environmental conditions.

Practical Application of the Aqueous ‘Sulfonyl-Azide-Free’ (SAFE) Diazo Transfer Protocol to Less α-C–H Acidic Ketones and Esters

The earlier described ‘sulfonyl-azide-free’ (‘SAFE’) protocol for diazo transfer to CH-acidic 1,3-dicarbonyl compounds (and their similarly activated congeners) has been extended to the less reactive monocarbonyl substrates, which previously required a separate activation step. Formylation in situ, followed by the addition of an optimized amount of the ‘SAFE cocktail’ (obtained by mixing sodium azide, potassium carbonate, and m-carboxybenzenesulfonyl chloride in water) led to the formation of the desired diazo compounds, which were isolated by extraction in moderate to excellent yields, and, in most cases, with no need for additional purification.

A wake-active locomotion circuit depolarizes a sleep-active neuron to switch on sleep

Sleep-active neurons depolarize during sleep to suppress wakefulness circuits. Wake-active wake-promoting neurons in turn shut down sleep-active neurons, thus forming a bipartite flip-flop switch. However, how sleep is switched on is unclear because it is not known how wakefulness is translated into sleep-active neuron depolarization when the system is set to sleep. Using optogenetics in C. elegans, we solved the presynaptic circuit for depolarization of the sleep-active RIS neuron during developmentally-regulated sleep, also known as lethargus. Surprisingly, we found that RIS activation requires neurons that have known roles in wakefulness and locomotion behavior. The RIM interneurons, which are active during and can induce reverse locomotion, play a complex role and can act as inhibitors of RIS when they are strongly depolarized and as activators of RIS when they are modestly depolarized. The PVC command interneurons, which are known to promote forward locomotion during wakefulness, act as major activators of RIS. The properties of these locomotion neurons are modulated during lethargus. The RIMs become less excitable. The PVCs become resistant to inhibition and have an increased capacity to activate RIS. Separate activation of neither the PVCs nor the RIMs appears to be sufficient for sleep induction; instead, our data suggests that they act in concert to activate RIS. Forward and reverse circuit activity is normally mutually exclusive. Our data suggest that RIS may be activated at the transition between forward and reverse locomotion states, perhaps when both reverse (including RIM) and forward (PVC) circuit activity overlaps. While RIS is not strongly activated outside of lethargus, altered activity of the locomotion interneurons during lethargus favors strong RIS activation and thus sleep. The control of sleep-active neurons by locomotion circuits suggests that sleep may have evolved from locomotion quiescence. The flip-flop sleep switch in C. elegans thus requires an additional component, wake-active sleep-promoting neurons that translate wakefulness into the depolarization of a sleep-active neuron when the worm is sleepy. Wake-active sleep-promoting circuits may also be required for sleep state switching in other animals including in mammals.

A one-step self-sustained low temperature carbonization of coconut shell biomass produced a high specific surface area biochar-derived nano-adsorbent

A one-step self-sustained carbonization of coconut shell biomass, carried out in a brick reactor at a relatively low temperature of 300–500°C, successfully produced a biochar-derived adsorbent with 308 m2/g surface area, 2 nm pore diameter, and 0.15 cm3/g total pore volume. The coconut shell biochar qualifies as a nano-adsorbent, supported by scanning electron microscope images, which showed well-developed nano-pores on the surface of the biochar structure, even though there was no separate activation process. This is the first report whereby coconut shell can be converted to biochar-derived nano-adsorbent at a low carbonization temperature, without the need of the activation process. This is superior to previous reports on biochar produced from oil palm empty fruit bunch.

N-Acylation of Oxazolidinones via Aerobic Oxidative NHC Catalysis

<p>In the ongoing quest to find alternatives to atom un-economical and forcing conditions in acylation reactions, aerobic oxidative NHC catalysis has emerged as a method to convert aldehydes to potent acylating reagents. This strategy has been utilized in the esterification of alcohols but not yet been shown for densely polyfunctionalized <i>N</i>- heterocycles such as, oxazolidinones and pyrrolidinones. Conventional acylation of oxazolidinones are typically associated with forcing reaction conditions, requiring separate activation steps and strong bases, which does not adhere to the principles of green chemistry. For reasons of waste prevention, atom economy, less hazardous syntheses and reduction of derivatives finding alternative methods are desirable.</p><p> </p>In this manuscript, we demonstrate the synthesis of several <i>N</i>-acylated oxazolidinones and pyrrolidinones that are chemically relevant, both found as pharmaceuticals and natural products as well as auxiliaries for synthesis. The developed method operates at room temperature and can be performed in ethyl acetate with open reaction vessels. The substrate scope is broad, with products isolated in good to excellent yields. The functional group tolerance is exemplified with 22 entries, where different aldehydes, oxazolidinones and pyrrolidinones are systematically investigated. Moreover, the reaction is clean as water is generated as the only byproduct.

N-Acylation of Oxazolidinones via Aerobic Oxidative NHC Catalysis

<p>In the ongoing quest to find alternatives to atom un-economical and forcing conditions in acylation reactions, aerobic oxidative NHC catalysis has emerged as a method to convert aldehydes to potent acylating reagents. This strategy has been utilized in the esterification of alcohols but not yet been shown for densely polyfunctionalized <i>N</i>- heterocycles such as, oxazolidinones and pyrrolidinones. Conventional acylation of oxazolidinones are typically associated with forcing reaction conditions, requiring separate activation steps and strong bases, which does not adhere to the principles of green chemistry. For reasons of waste prevention, atom economy, less hazardous syntheses and reduction of derivatives finding alternative methods are desirable.</p><p> </p>In this manuscript, we demonstrate the synthesis of several <i>N</i>-acylated oxazolidinones and pyrrolidinones that are chemically relevant, both found as pharmaceuticals and natural products as well as auxiliaries for synthesis. The developed method operates at room temperature and can be performed in ethyl acetate with open reaction vessels. The substrate scope is broad, with products isolated in good to excellent yields. The functional group tolerance is exemplified with 22 entries, where different aldehydes, oxazolidinones and pyrrolidinones are systematically investigated. Moreover, the reaction is clean as water is generated as the only byproduct.

Emotional Labor Predicts Service Performance Depending on Activation and Inhibition Regulatory Fit

When service providers regulate their moods and expressions (i.e., deep acting and surface acting), are they better performers? Drawing on the framework of activation-inhibition regulatory systems and regulatory fit, we propose (a) that deep acting represents an activation-oriented regulation strategy and surface acting, an inhibition-oriented regulation strategy; (b) that these strategies have separate pathways to desirable performance (i.e., affective delivery) and counterproductive performance (i.e., service sabotage), respectively; and (c) that performance is optimized when momentary regulation strategies are aligned with activation- and inhibition-oriented traits. Empirically, across two studies, we employ a multilevel approach (i.e., within- and between-person), a multisource approach (i.e., self, coworker, customer), and a multicontext approach (i.e., banks and restaurants) to test regulatory fit as applied to emotional labor. In two studies, we support separate activation and inhibition pathways, plus regulatory fit, in that deep acting is beneficial to affective delivery for those higher in two activation traits—namely, extraversion and openness—and that surface acting predicts service sabotage for those lower in an inhibition trait: conscientiousness. We empirically rule out mood as the explanation for these effects, propose future research to apply regulatory fit to other outcomes and contexts, and suggest practical implications for services.