The organizational culture at the nation level is integrated by values, attitudes, workforce, loyalty, interpersonal relationships, etc. of its members. In the present investigation, the key to the business success of Mexico and the United States taking into consideration an element of analysis for its achievement is explained, making a comparison between both countries. There is a national culture with greater impact on organizations in the US than in Mexico. The methodology used was qualitative, exploratory, descriptive, and comparative. This research shows that the national culture affects the ways of acting of the members of the companies. The study was qualitative using only secondary sources for the investigation.